Objective To investigate the effect of resveratrol (RES)on sevoflurane-induced nerve cell injury and its mechanism.Methods Fetal hippocampal tissues were taken from SD pregnant rats and primary nerve cells were isolated and cultured,then the cells were randomly divided into four groups:group control (group C),normal primary nerve cells;group 3% sevoflurane (group S),cells treated with 3% sevoflurane gas;group 3% sevoflurane+RES (group SR),cells treated with RES for 6 h,and then treated with 3% sevoflurane gas;group RES (group R),cells treated with RES.Flow cytometry assay was used to detect the apoptosis of nerve cell;CCK-8 Kit was used to detect cell proliferation;Western blot was used to detect the expression level of Caspase-3,Bax,Bcl-2 and Bace-1;ELISA method was used to detect the protein level of amyloid precursor protein (APP)andβ-amyloid peptide (Aβ).Results Compared with the group C,cell apoptosis was increased (P <0.05),cell proliferation was decreased (P <0.05),the expression levels of Caspase-3 and Bax were up-regulated (P <0.05),the expression level of Bcl-2 was down-regulated (P <0.05), and the protein levels of Bace-1,APP and Aβwere significantly decreased by 3% sevoflurane treat-ment (P <0.05),which were all significantly reversed by RES treatment.Compared with group S, cell apoptosis was decreased (P <0.05),cell proliferation was increased (P <0.05),the expression levels of Caspase-3 and Bax were down-regulated (P <0.05),the expression level of Bcl-2 was up-regulated (P <0.05),and the protein levels of Bace-1,APP and Aβwere increased in group SR (P <0.05).Conclusion Resveratrol alleviated sevoflurane-induced nerve cell injury in vitro by reducing cell apoptosis and promoting cell proliferation,which may be related with the reduction of Aβexpression and the reduced Aβ-induced neurotoxicity.

AIM: To explore the influence of clonidine on inflammatory response in lung injury mice and its possible mechanism.METHODS: Clonidine solution was intravenously injected into the mice with lung injury induced by LPS.The left upper lobe of the lung was collected to detect lung wet/dry weight ratio (W/D) and total lung water content (TLW).The concentrations of IL-6, IL-1β and TNF-α were measured by ELISA.The expression of α7 nicotinic acetylcholine receptor (α7nAChR) and high-mobility group box protein 1 (HMGB1) at mRNA and protein levels was determined by RT-PCR and Western blot.After importing α7nAChR siRNA lentiviral vector or injecting exogenous HMGB1 protein, the inflammatory cytokines were detected.RESULTS: Clonidine attenuated lung injury and inhibited inflammatory reaction.Clonidine promoted the activation of cholinergic anti-inflammatory pathway by promoting α7nAChR expression.Clonidine inhibited HMGB1 expression, which promoted the secretion of IL-6, IL-1β and TNF-α.HMGB1 was negatively regulated by α7nAChR.CONCLUSION: Clonidine functions as an anti-inflammatory reagent to the lung injury mice.The mechanism may be related to activating the cholinergic anti-inflammatory pathway and inhibiting the expression of HMGB1.

At the moment of its WTO accession, China also enters the stage of mass higher education. Mass higher education is an objective inevitability of the economic and social development. China's mass higher education lays a firm foundation for China to join the WTO and the WTO entry in turn brings considerable opportunities to it. It is expected that the WTO entry will promote the process of mass higher education of China.

Ambulatory blood pressure monitor (ABPM) was done on 103 hypertensive patients according to clinical stages and left ventricular measure (LVM) was randomly done on a portion of the patients. The results showed that the variability of stage-Ⅱ-group's 24 hour systolic pressure and stage-Ⅲ-group's systolic and diastolic pressures was significantly higher than that of stage-Ⅰ-group (P

Objective To determine the content of Ginsenoside Rb1 by RP-HPLC. Methods HPLC was employed with a column of Diamonsil ODS-C18 and a mobile phase of Acetonitrile-H2O (29∶71), the detection wavelength at 203 nm and the column temperature at 25 ℃. Results The linear correlation is available between 0.776 and 7.760 ?g/mL of Ginsenoside Rb1, and the correlation coefficient was 0.999 99, the average recovery was 99.69% and RSD was 2.04% (n=6). Conclusion The method is simple and accurate, and applicable to control the quality of Chanshu Granule.

Objective To assess the effects of three way catalytic converter on the health risk of benzene series.Methods After collecting the benzene series(benzene,toluene,xylene) in the air inlet and air outlet of the three way catalytic converser in 100% working loads,their health risk was assessed with the methods of health risk assessment.Results After catalysis,the hazard index of toluene and xylene decreased in all loads,the hazard index and carcinogenesis risk of benzene increased in 100% loads,the chronic long-term hazard index of benzene deceased in 100% loads.Conclusion The three way catalytic converter can change the health risk of benzene series emission by gasoline engine,the carcinogenesis risk of benzene series emission will be increased when the gasoline is treated with the three way catalytic converter.

Objective To investigate the imaging features of hypertrophic spinal pachymeningitis(HSP).Methods 2 patients with HSPproved pathologically received both CT and MRI scan. The literatures were reviewed, the clinical data and imaging features of HSP wereretrospectively analyzed.Results The characteristic HSP manifestation of HSP on imaging was the crescent-shaped thickening of spinaldura maters , the thickness of the dura was 5.2 mm and 13 mm respectively in this two cases . Conclusion HSP is of typical imaging characteristic. The diagnosis can be made correctly in combination with clinical data.

Objective To study the effect of zinc on lipid peroxidation, anti-oxidase and development of rats. Methods 64 SD rats weight was(109.7?10.4) g, 32 male, 32 female were randomly divided into 4 groups. Every group was fed on the fodder contained zinc of different content for 40 days to build animal model. The Zn, Cu, Fe and MDA contents, SOD and GSH-Px activity in tissue of rats were determined. The One-way ANOVA test was used for multiple related samples and simple correlation analysis was used. Results Compared with 30 mg/kg group, the development of thighbones and the body weight of rats in groups with zinc exposure doses of 9 and 400 mg/kg bw were significantly retarded, the consumption rate of fodder was lower in male rats (P

The development of gastrointestinal minimally invasive surgery in China is changing during the past decade.The Chinese laparoscopic gastrointestinal surgery study (CLASS) group as the earliest and most closely cooperated organization for surgical clinical researches,under the support of Chinese Medical Association,Chinese Anti-cancer Association and Chinese Medical Doctor Association,the clinical research capacity and minimally invasive technology of the CLASS group continue to be developed.With the launch of the prospective,randomized,controlled CLASS-01 trial for the treatment of locally advanced gastric cancer in 2012 and the publication of safety results of laparoscopic surgery for gastric cancer in 2016,the international academic community is increasingly focusing its attention on China.This article attempts to review the history and development of the CLASS group in the past 7 years,aiming to prospect the future and attempt to create trends.

Objective To investigate the clinical effect of three dimensional conformal radiation therapy combined with EP regimen for limited-stage of small cell lung cancer in elderly.Methods A total of 83 elder patients with limited-stage of small cell lung cancer were divided into treatment group and control group by random digits table method.In treatment group,42 patients received three dimensional conformal radiation therapy with synchronized EP regimen chemotherapy.In control group,41 patients received 2 cycles of EP regimen chemotherapy in advance,then received three dimensional conformal radiation therapy.Results The complete remission rate and objective response rate of treatment group and control group had significant differences [45.2％ (19/42) vs.17.1％ (7/41),81.0％ (34/42) vs.51.2％ (21/41)] (P =0.006,0.004).Follow-up for 2 years,there were 2 patients lost in treatment group,there was 1 patient lost in control group.The 1,2 years survival rate of treatment group and control group had significant difference [87.5％(35/40) vs.60.0％(24/40),65.0％(26/40) vs.32.5％(13/40)](P =0.005,0.004).The incidence of leukopenia,anemia,radiation pneumonitis and radiation esophagitis in treatment group were higher than those in control group,but there were no significant differences (P＞ 0.05).The 2-year local recurrence rate and distant metastasis rate of treatment group and control group had significant differences [17.5％(7/40) vs.42.5％ (17/40),32.5％ (13/40) vs.62.5％ (25/40)] (P =0.015,0.007).Conclusions Three dimensional conformal radiation therapy combined with EP regimen for limited-stage of small cell lung cancer in elderly can improve the short-term efficacy,long-term survival and reduce the rate of local recurrence and distant metastasis.It is safe and effective in the treatment of limited-stage of small cell lung cancer in elderly.