Objective To observe the relation between content of type Ⅲ collagen and expression of decorin in paraurethral connective tissue in patients with stress urinary incontinence(SUI). Methods Transvaginal biopsies were obtained from the paraurethral connective tissue in 43 SUI and non-SUI control women. The concentration of type Ⅲ collagen was determined by immunohistochemical technique, electrophoretic separation and quantification. RT-PCR was used to verify the mRNA level of decorin. Results The mean concentration of type Ⅲ collagen was significantly reduced in patients with SUI(23?4), compared to the control group(34?6, P

Traditional sequences of magnetic resonance imaging (MRI) such as T1-weighed imaging, T2-weighed imaging, and fluid-attenuated inversion recovery (FLAIR) imaging can sensitively detect the subacute and chronic intracranial hemorrhage; gradient echo sequence can detect various stages of intracranial hemorrhage, but there is no correlation between the signal intensity of the hematoma and the time of the presence of hematoma. Diffusion-weighted imaging and apparent diffusion coefficient may provide more information about the center and surrounding areas of hematoma, as well as the hemorrhagic tendency after infarction.

Objective To investigate the prevalence of lower urinary tract symptoms in women in a community in Fuzhou. Methods We designed a patient-completed questionnaire according to Bristol Female Lower Urinary Tract Symptoms (BFLUTS) questionnaire.A few parts of the questionnaire were adjusted based on living habits and social backgrounds in our country.The health care workers distributed and collected the questionnaires and instructed the women to fill in them one by one. Results A total of 6066 questionnaires were distributed and 5587 were collected. Of the responders 4745 women were more than 18 years. The prevalence of stress incontinence, urgency incontinence,urgency,frequency and nocturia were 9.0% (425/4745),2.4% (113/4745),10.3% (489/4745),1.5% (72/4745) and 9.8% (446/4745),respectively.The prevalence of storage symptoms increased with age,showing a 20.5%(104/507) in 18~27 years’ group,19.1% (332/1740) in 28~37,22.5%(338/1503) in 38~47,32.2%(202/628) in 48~57,41.2%(61/148) in 58~64,58.0% (127/219) in ≥65 years’ group. The overall prevalence was 24.5%.The prevalence of delayed urinary,intermittent stream,increased abdominal urination, incomplete emptying and voiding pain were 7.0%(330 cases),4.1%(194),4.6% (217),4.9% (233) and 4.8%(229),respectively.The prevalence of general voiding dysfunction increased with age,showing a 13.0% (66/507) in 18~27 years’ group,9.3% (162/1740) in 28~37,10.7%(161/1503) in 38~47,13.2%(83/628) in 48~57,16.2% (24/148) in 58~64 and 26.9%(59/219) in ≥65 years’ group.The overall prevalence was 11.7%. Conclusions The prevalence of lower urinary tract symptoms are high in community-dwelling women,and the prevalence of storage symptoms are more common than that of voiding dysfunction in these women.

[Objective]To evaluate the surgical techniques,rehabilitation and the results of internal fixation performed for ankle joint fracture.[Method]One hundred and twenty-six patients with ankle joint fractures were treated with open reduction and internal fracture. According to the Danis-Weber classification,there were 12 cases of type A, 86 cases of type B, 28 cases of type C.The follow-up interview period varied from one year to five years,with an average of eighteen months.[Result]The number of patients whose results was better, good, fair and poor was 65, 44, 11 and 6.The total percent-age of good to better clinical results was 86.51%.[Conclusion]Operative treatment and rehabilitation may provide satisfactory fracture reduction and clinical results for ankle joint fracture.The opportunity selection,correct fracture pattern estimation, proper internal fixation and rehabilitation are important to achieve better long term results.

[Objective]To investigate the clinical use of spiral CT with 3D Reconstruction for fractures of the tri-ankle joint.[Method]Fifty-six cases of tri-ankle joint fractures treated in our hospital from 1999 to 2004 were studied respectively.The ankle joint X-ray and spiral CT with 3D reconstruction were taken for all the cases preoperatively.The results were analyzed and compared with finding in operation .Scanning parameters of spiral CT were: 2 to 5mm collimation,3 to 5mm table speed and 1.5 to 3mm reconstruction interval.[Result]Fractures were demonstrated definitely by spiral CT in all the cases.Analyses of the results showed the superiority of spiral CT with 3D reconstruction in the fracture classification.Exact assessment of fragments and total agreement of the preoperative results were compared with findings in operation.[Conclusion]Operative treatment and rehabilitation may provide satisfactory fracture reduction and clinical results for ankle joint fracture.Well-timed treatment,correct fracture pattern estimation,proper internal fixation and rehabilitation are important to achieve better long term results.

0 05) While patients′ satisfactory rate was significantly higher in electrical stimulation group than in medical group( P

Aim To investigate the effect of orientin on proliferation and differentiation of 3T3-L1 pre-adipocytes and on insulin resistance(IR) in 3T3-L1 adipocytes and the possible mechanisms.Methods MTT assay and oil red O staining were applied to investigate the proliferation and the differentiation of 3T3-L1 pre-adipocytes, respectively.The intracellular triglyceride(TG) contents were detected by enzymatic analysis.IR model was induced with dexamethasone.A fluorescent glucose analogue, 2-NBDG, was used to measure the rate of glucose uptake.Western blot was used to detect the protein level of GLUT4 and phosphorylation of AMPK and ACC.The GLUT4 translocation was measured by fluorescent-immunohistochemistry.Results Orientin decreased the formation of lipid droplets and intracellular TG contents(P<0.01) in a concentration-dependent manner(P<0.05), but it had no obvious effects on the cell vitality.Under the IR state, orientin significantly increased 3T3-L1 adipocytes glucose uptake(P<0.05).Meanwhile, orientin up-regulated the protein expression of p-AMPK, p-ACC, and enhanced GLUT4 translocation and its expression.Conclusion Orientin can effectively inhibit the differentiation of 3T3-L1 pre-adipocytes and increase insulin sensitivity due to the activation of AMPK/GLUT4 signal pathway.

Objective To analyze re-treatments of recurrence after the pelvic floor repair surgery.Methods The protocol and the effect of re-treatments were investigated by reviewing and analyzing the clinical data of 81 recurrent patients (grade Ⅱ and above),who had received the pelvic floor repair surgery from January 2011 to January 2016.Pelvic organ prolapse quantitation system (POP-Q) and two questionnaires about quality of life [pelvic floor distress inventory-short form 20 (PFDI-20) and pelvic floor impact questionnaire short form (PFIQ-7)] were used to evaluate objective and subjective efficacy,respectively.Results Among 81 recurrent patients who were followed up for a median of 35 months (10-69 months),78 cases (with prolapse up to grade Ⅲ or Ⅳ) were treated by surgical operation with both objective cure rate and subjective satisfaction being 100％ (78/78);3 cases (with grade Ⅱ prolapse) were treated by pelvic floor electrical stimulation biofeedback,and 1 case among the three cases had the vaginal foreign body sensation,the subjective satisfaction was 2/3.The methods of surgical operation for the 78 recurrent patients included:total pelvic floor reconstructive surgery (55 cases;3 of which involve trachelectomy),anterior pelvic reconstructive surgery (2 cases),posterior pelvic reconstructive surgery (3 cases),Y-mesh sacral colpopexy (2 cases),colpocleisis (11 cases),vaginal hysterectomy combined posterior fornix forming (3 cases),and vaginal hysterectomy combined posterior pelvic reconstructive surgery (2 cases).Conclusion The extent of recurrence,the recurrent site and complications must be carefully considered and evaluated for re-treatments of recurrence after pelvic floor repair surgery,and then an appropriately individualized re-treatment protocol could be designed for each of the patients.

Lipase from Burkholderia cepacia complex is one of the most versatile biocatalyst and is used widely in many biotechnological application fields including detergent additives, the resolution of racemic compounds, etc. Based on the known whole genomic information of B. cepacia, both ampicillin and kanamycin were added to the TB-T media, the traditional selective media, to screen B. cepacia complex strains from rhizosphere soil samples. The single colonies on the plates with the modified TB-T media were then qualitatively determined the ability to produced the extracellular lipase in the rhodamine B-olive oil agar plates. Thirty-five strains of lipolytic pseudo-B. cepacia complex were isolated and the positive rate of lipolytic bacteria was 65%. Among them, 15 pseudo-B. cepacia complex strains with the tolerance to benzene, n-hexane and n-heptane at the concentration of 10% (V/V) were selected and identified by the recA gene sequence. All of the 15 lipolytic bacteria belonged to the B. cepacia complex.

Object To investigate the berberine in Coptis chinensis Franch. processed with various quantity of Evodia rutaecarpa (Juss.) Benth. and the components absorbed from E. rutaecarpa. Methods Taking berberine, evodiamine and rutaecarpine as targets, HPLC method was used to determine the components of C. chinensis, E. rutaecarpa, C. chinensis processed with 10%, 20%, 30%, 40%, 50% E. rutaecarpa. Results The content of berberine in C. chinensis processed with E. rutaecarpa decreased, and that of C. chinensis processed with 20% and 30% E. rutaecarpa was higher than the rest. C. chinensis processed with E. rutaecarpa absorbed the components of E. rutaecarpa really. Conclusion The suitable quantity of E. rutaecarpa is 20% when processing for C. chinensis.