Objective To analyze the clinical distribution and antimicrobial resistance of Acinetobacter baumannii (A.baumannii)from a hospital,so as to guide the rational use of antimicrobial agents and provide scientific basis for the prevention and control of healthcare-associated infection(HAI).Methods Distribution and antimicrobial susceptibility testing results of A.baumannii isolated from patients between January 2011 and December 2013 were analyzed retrospectively.Results A total of 339 A.baumannii isolates were isolated within three years,291 iso-lates (85.84%)were from sputum,followed by urine (n=29,8.55%),secretion(n=12,3.54%)and blood (n=3,0.89%).The main distribution departments were intensive care unit(ICU,n=149,43.95%),department of re-spiratory medicine (n=46,13.57%),department of pediatrics (n =32,9.44%)and oncology (n =22,6.49%). The resistant rate of A.baumannii to tobramycin and imipenem were 11 .11 %-49.47%,the resistant rates to the other antimicrobial agents were relatively high;antimicrobial resistant rate of 2012 and 2013 were obviously lower than 2011 .Conclusion A.baumannii from this hospital are mainly from sputum specimens,and mainly distribute in ICU and department of respiratory medicine,antimicrobial resistant rate is high,but there is a decreasing tenden-cy in recent years.

Objective To investigate the inhibitory effects of hepatitis B virus (HBV) S gene-specific antisense locked nucleic acid (LNA) on HBV replication and expression in HepG_22.2.15 cells,and to screen the effective short sequence of LNA.Methods Four different lengths of short sequence of antisense locked nucleic acid which were complementary to the initiator of HBV S gene were designed,synthesized and transfected by cationic liposomes into HepG_22.2.15 cells.The HBsAg and HBV DNA of supematant was tested by enzyme linked immunoadsorbent assay(ELISA) and real-time fluorescent quantitative PCR(FQ-PCR) at 24,48 and 72 hours after treatment.LNA's cyto-toxicity on cell was evaluated by MTT method.Results Four different lengths of short sequence of LNA inhibi-ted the expression of HBsAg and the replication of HBV DNA with the inhibition rates of 46.58%,54.38%,72.43% ,69.92% and 27.09% ,28.77% ,34.71% ,32.68% respectively after 72 hours.There's no obvious tox-icity on cell.Conclusion Antisense LNA that targeting at HBV S gene has strong inhibition on HBV in vitro,and the optimal length of LNA sequence might be in the range of 15 base to 25 base.It has a therapeutic potential in the treatment of patients infected with HBV.

To study the effects of salidroside-pretreatment on changes of neuroethology in rats with global cerebral ischemia-reperfusion injury so as to investigate its probable mechanism.

OBJECTIVE: To determine the expression of MTA1 and RECK gene in nasopharyngeal carcinoma (NPC) and its correlation with clinicopathological features. METHOD: In situ hybridization was used to detect the expression of MTA1 and RECK mRNA in 60 cases of primary NPC (30 cases with, 30 cases without cervical lymph node metastasis), 10 cases of metastatic lymph node (MLN) and 20 cases of chronic nasopharyngitis tissue (CNT). RESULT: 1) Positive rates of MTA1 mRNA expression in CNT, NPC and MLN were 30.0% (6/20), 71.7% (43/60) and 80.0% (8/10), respectively. The positive expressions of MTA1 in NPC and MLN were significantly higher than in CNT (P<0.05). The MTA1 mRNA expression in NPC with lymph node metastasis was significantly higher than in NPC without lymph node metastasis (83.3% vs. 60.0%, P<0.05). 2) Positive rates of RECK mRNA expression in CNT, NPC and MLN were 85.0% (17/20), 26.7% (16/60) and 30.0% (3/10), respectively. The positive expression of RECK in NPC and MLN were significantly lower than in CNT (P< 0.05). The RECK mRNA expression in NPC with lymph node metastasis was significantly lower than in NPC without lymph node metastasis (13.3% vs. 40.0%, P<0.05). 3) Abnormal expression of MTA1 and RECK mRNA in NPC had no correlation with gender, age, T-stage and clinical stage (P>0.05), while they had positive relationship with lymph node metastasis, tumor recurrence and the post-treatment 5-year survival periods of NPC (P<0.05). The expression of RECK mRNA was negatively correlated with the expression of MTA1 mRNA in NPC (r = - 0.541, P<0.05). CONCLUSION These data suggest that the aberrant expression of MTA1 and RECK gene may be involved in the invasion and metastasis of NPC. They may be good biomarkers for evaluating the cervical lymph node metastasis, recurrence and prognosis of NPC.
Adult ; Aged ; Carcinoma ; genetics ; pathology ; Female ; GPI-Linked Proteins ; genetics ; Histone Deacetylases ; genetics ; Humans ; In Situ Hybridization ; Lymphatic Metastasis ; Male ; Middle Aged ; Nasopharyngeal Carcinoma ; Nasopharyngeal Neoplasms ; genetics ; pathology ; Neoplasm Staging ; Repressor Proteins ; genetics

OBJECTIVE: To investigate the expression and the relativity of RECK,RAGE and MMP-9 in nasopharyngeal carcinoma (NPC) tissues. METHOD: RECK, RAGE and MMP-9 were detected with immunohistochemical methods in 64 NPC specimens(30 cases with cervical lymph nodes metastasis, while the other 34 cases are not) and 30 specimens with chronic nasopharyngitis. RESULT: RECK hardly expressed in chronic nasopharyngitis and NPC tissues, However, strong expression of RECK could be seen in the surrounding inflammatory cells and matrix of cancer nests. The positive rates of RECK in NPC with and without cervical lymph nodes metastasis were 3.3% (1/30) and 11.8% (4/34), separately, There was no statistical significance between the groups (P > 0.05). The positive rates of RAGE in chronic nasopharyngitis and NPC tissues were 100% (30/30) and 70.3% (45/64) respectively, while MMP-9 were 46.7% (14/30) and 78.1% (50/64). The positive rates of RAGE in NPC with and without cervical lymph nodes metastasis were 86.7% (26/30) and 55.9% (19/34) separately, whereas MMP-9 were 90.0% (27/30) and 67.7% (23/34) respectively. They all showed statistical significance between the groups. MMP-9 had a negative correlation with RECK (r = -0.369, P < 0.05) and a positive correlation with RAGE in NPC (r = 0.471, P < 0.05). CONCLUSION The expression of RECK and RAGE in NPC were down-regulated, the expression of MMP-9 was up-regulated. While the expression of RECK in NPC with cervical lymph nodes metastasis was down-regulated, while RAGE and MMP-9 were up-regulated. The abnormal expression of the three genes may be related to the progression of NPC. RECK and RAGE may be involved in the invasion and metastasis of NPC by regulating the expression of MMP-9.
Adolescent ; Adult ; Aged ; Female ; GPI-Linked Proteins ; metabolism ; Glycation End Products, Advanced ; metabolism ; Humans ; Male ; Matrix Metalloproteinase 9 ; metabolism ; Middle Aged ; Nasopharyngeal Neoplasms ; metabolism ; pathology ; Neoplasm Invasiveness ; Neoplasm Metastasis ; RNA, Messenger ; genetics ; Receptor for Advanced Glycation End Products ; metabolism ; Receptors, Cell Surface ; biosynthesis ; Young Adult

Objective To compare two different methods to detect the differences of gene mutation rate, sensitivity, specificity and coincidence rate of epidermal growth factor receptor (EGFR) in non-small-cell lung cancer (NSCLC) so as to assess the clinical value of qRT-PCR method and its environmental-friendly technologyplatforms.One uses environmental fixative poly hydroxyl acrylic acid and green transparent liquid dewaxing Van-clear alone or in combination to replace the traditional fixative 4％ (volume fraction) neutral buffered formalin and the traditional transparent dewaxing liquid xylene in application of quantitative real-time polymerase chain reaction (qRT-PCR).The other uses traditional reagents in direct sequencing.Methods We selected 91 cases of primary NSCLC specimens resected between May 2013 and March 2016 in Zhongshan Bo`ai Hospital and Zhongshan Hospital of Traditional Chinese Medicine.Five samples were taken from the same tumor lesion.We used a random number table to randomly divide these samples into Groups A, B , C, D, and E.Group A received direct sequencing method in detection of EGFR gene mutations.Besides, during the experiment, 4％ neutral buffered formalin was used for fixing, and xylene transparent dewaxing was used to make slices for DNA extraction dewaxing.Group B received qRT-PCR method to detect EGFR gene mutations.Meanwhile, during the experiment, 4％ neutral buffered formalin was used for fixing, and xylene transparent dewaxing was used to make slices for DNA extraction dewaxing.Group C received qRT-PCR method in detection of EGFR gene mutations.At the same time, during the experiment, polyhydroxy acrylic acid was used for fixing, and xylene transparent dewaxing was used to make slices for DNA extraction dewaxing.Group D received qRT-PCR method to detect EGFR gene mutations.In the meantime, 4％ neutral buffered formalin was used for fixing, Van-clear transparent dewaxing was used to make slices for DNA extraction dewaxing.Group E received qRT-PCR method in detection of EGFR gene mutations.In addition, during the experiment, polyhydroxy acrylic acid was used for fixing, and Van-clear transparent dewaxing was used to make slices for DNA extraction dewaxing.In addition, during the experiment, polyhydroxy acrylic acid was used for fixing, and Van-clear transparent dewaxing was used to make slices for DNA extraction dewaxing.The mutations of Exons 18, 19, 20, and 21 in EGFR genes were respectively determined in the five groups of NSCLC.Results ① Groups B, C, D, E and A did not significantly differ in the percentage of people with mutations or target site mutation rates of EGFR genes in NSCLC (P> 0.05).② The detection results of EGFR target site mutation in Groups B, C, D, E and A had good sensitivity, strong specificity, and high compliance rate.Conclusion The green transparent liquid dewaxing Van-clear alone or in combination to replace the traditional fixative 4％ neutral buffered formalin and the traditional transparent dewaxing liquid xylene in the application of qRT-PCR so as to detect EGFR gene mutations in NSCLC has good consistent results compared with the method that uses traditional reagents in direct sequencing.It has the significance and value in clinical application.

Whether the localized flow acceleration occurs in the resting stenotic left anterior descending coronary artery was explored and its value for detection of coronary stenosis estimated.Blood flow in the left anterior descending coronary arteries in 45 patients was detected by transthoracic color Doppler echocardiograph and multipoint pulse Doppler spectrums were recorded in the same segment. The ratio of the maximal peak diastolic velocity to the minimal peak diastolic velocity was calculated. The ratio ≥1.5 was the cutoff value for the presence of localized acceleration flow.There were 23 patients with localized acceleration flow examined by echocardiography. Twenty of them were found to have luminal diameter stenosis (60 %- 98 %) in the left anterior descending coronary arteries by coronary angiography and 3 patients were normal. There were 22 patients without localized acceleration flow examined by echocardiography. Eighteen of them had no or ＜60% stenosis. Four patients had serious stenosis (≥95 %) or occluded segments in the left anterior descending coronary arteries on coronary angiography. The ratio of the maximal peak diastolic velocity to the minimal peak diastolic velocity was significantly higher in patients with left anterior descending coronary artery stenosis than that in those without stenosis (1.9±0.3 vs 1.3±0.2, P＜0.01) and it correlated significantly with left anterior descending coronary artery stenosis (r=0.77, P＜0.01). The specificity by using the ratio≥1.5 for stenosis detection was 85.7 % (18/21), and the sensitivity was 83.3 % (20/24). This study demonstrated that local blood flow velocity was increased in the resting stenotic left anterior descending coronary artery. Transthoracic color Doppler echocardiography is a reliable noninvasive method to detect localized acceleration flow in the left anterior descending coronary artery stenosis and it is useful in the noninvasive diagnosis of stenosis in the left anterior descending coronary artery.

Objective:To develop a quantitative HPLC-MS/MS method for the determination of astragulin and kaempferol in Bairui granule. Methods:A column of Ultimate XB-C C18(100 mm×2.1 mm,5 μm)was used. The mobile phase was composed of 0.1% formic acid-water(A) and 0.1% formic acid-acetonitrile(B) with gradient elution. The flow rate was 0.3 ml·min-1. The detection was performed with multiple teactions monitoring (MRM) using electrospray ionization (ESI). The precursor/product ion transitions were monitored at m/z 447.2→m/z 284.0 (anion mode) for astragulin and m/z 285.1→m/z 187.1 (anion mode) for kaempferol. The temperature of column was set at 40℃. Results:A good linearity was obtained with the concentration ranging from 9.86 to 1 970 ng·ml-1(r=0.999 5) for astragulin and 8.73 to 437 ng·ml-1(r=0.999 6) for kaempferol. The average recovery of the two inves-tigated compounds at low, medium and high concentrations was 99.23%, 99.65% and 99.23%, and 98.24%, 99.13% and 99.69% with the RSD of 1.84%,1.37% and 1.21%,and 1.38%,0.96% and 1.47%, respectively (n=6). Conclusion: The method is rapid,accurate and reliable,which can be used to control the quality of Bairui granule.

Objective:To study the evolution of Traditional Chinese Medicine (TCM) syndromes of 171 cases of Kawasaki disease (KD) under the intervention of gamma globulin therapy based on factor analysis.Methods:A cross-sectional study. 171 cases of KD children hospitalized in the Department of Cardiology of Guangzhou Women's and Children's Medical Center from July 2019 to December 2020 were collected. All patients were treated with intravenous gamma globulin (2 g/kg) for 1 week. According to the results of the treatment with C-ball, 171 children with C-ball sensitive KD were selected to collect the four diagnostic data, and the representative syndromes of defensive level, qi level, yin level, and nutritive level were observed. Factor analysis was used to analyze the evolution of syndrome in 171 children with KD c-ball sensitivity.Results:The result of factor analysis showed that the KMO statistics of 171 children with c-ball sensitivity before treatment was 0.792, and Bartley test was significant ( P<0.01). 16 common factors were extracted, and 23 syndromes were screened, mainly including defensive level disorder, qi level disorder,nutritive level disorder,yin level disorder, heat stagnation and blood stasis syndrome. One week after treatment, the statistic of KMO test was 0.787, and Bartley test was significant ( P<0.01). 9 common factors were extracted, and 10 syndromes were screened, mainly including qi deficiency syndrome, yin deficiency syndrome and blood stasis syndrome. Conclusion:Before treatment, the TCM syndromes in KD C-cell sensitive children are mainly nutritive level disorder, defensive level disorder, qi level disorder yin level disorder, and heat stagnation and blood stasis syndrome; after treatment, the main TCM syndromes are mainly qi deficiency syndrome, yin deficiency syndrome and blood stasis syndrome.

Objective:To explore the HIV-1 drug resistance in patients with HIV/AIDS in Guilin city following the failure of antiretroviral treatment (ART).Methods:Plasma samples were collected from patients in Guilin who had received ART for more than 1 year and had a HIV viral load greater than or equal to 1 000 copies/ml from January 2019 to December 2023, and demographic information was also collected for HIV-1 genotype subtype analysis and drug resistance testing to determine the resistance mutation loci and the susceptibility of the strains to drugs.Results:A total of 766 patient samples with failed ART collection and successful amplification were collected, of which 536 (69.97%, 536/766) were male, with an average age of 53 years; a total of 8 HIV-1 subtypes were detected, with CRF01_AE (80.55%, 617/766), CRF07_BC (11.10%, 85/766) and CRF08_BC (6.92%, 53/766) predominated. The drug resistance analysis showed that the HIV-1 drug resistance rate was 34.86% (267/766), including nucleoside reverse transcriptase inhibitors (NRTIs), non-nucleoside reverse transcriptase inhibitors (NNRTIs) and protease inhibitor (PI), with dual resistance to NRTIs/NNRTIs (48.31%, 129/267) and NNRTIs resistance (43.07%, 115/267) predominantly. A total of 37 resistance mutation sites were detected, 14 NRTIs-associated mutation sites mainly included M184V/I (47.57%, 127/267), K65R (18.73%, 50/267), K70E/N/T/G/R (13.11%, 35/267), etc., and 18 NNRTIs-associated mutation sites mainly included K103 N/R (56.93%, 152/267), V179 D/E/T (21.72%, 58/267), G190C/S/Q (17.23%, 46/267), and V106I/M (16.85%, 45/267), etc.; and 5 PIs-associated mutation sites was the highest with L10V/I mutation rate (3.00%, 8/267).Conclusions:HIV/AIDS patients in Guilin have shown favorable outcomes in antiviral therapy, with a relatively low overall incidence of drug resistance. However, it is essential to enhance surveillance to reduce the spread of drug-resistant strains in the future.