Objective: To establish the determination method of adenosine in Compound Houtou Capsule. Methods: HPLC was performed to determine adenosine on DISCOVERY column, using methanol-0.05 mol/L potassium dihydrogen phosphate solution (13∶87,v∶v) as a mobile phase and detection wavelength at 259nm.Results: The linearity of this method was good, and the average recovery was 100.72%, RSD =0.22%. Conclusion: This method was simple and accurate, and available for the quality control of Compound Houtou Capsule.

AIM: To develop an HPLC method for analyzing the contents of paeoniflorin,baicalin,palmatine hydrochloride and berberine hydrochloride in Kuntai Capsules(Praeparata Radix Rehmannial,Rhizoma Coptidis,Radix Paeoniae,Radix Scutellariae,Colla Asini corii,Poria).METHODS: Four compounds were determirned with an Agilent ZORBAX SB C18 column(4.6 mm ? 250 mm,5 ?m).Acetonitrile-0.4% phosphoric acid was used as the mobile phase at a flow rate of 1.0 mL/min.The mobile phase was in linear gradient elution.The detection wavelength was 230 nm.RESULTS: The linear ranges were within 0.016 58-0.331 6 ?g(r = 0.999 9) for paeoniflorin,0.059 80-1.196 0 ?g(r = 0.999 9)for baicalin,0.012 91-0.258 2 ?g(r = 0.999 9)for palmatine hydrochloride,0.041 98-0.839 6 ?g(r = 0.999 9) for berberine hydrochloride.The average recoveries (n = 6) were 98.91% (RSD = 1.29% ),99.20% (RSD = 0.92% ),97.86% (RSD = 0.89% ),98.63% (RSD = 0.62% ),respectively.CONCLUSION: The method is simple,reliable,sensitive and can be applied to having controls over the preparation.

Objective To explore the effects of carbon monoxide(CO) on hydrogen sulfide(H_(2)S) / cystathionine-?-lyase(CSE) system in thoracic aorta of rats.Methods Twenty-seven thoracic aortae of 9 Wistar rats were divided into control group,hemin group(the final concentration of hemin was(10~(-4)mol/L)) and zinc protoporphyrin group(the final concentration of zinc protoporphyrin was(10~(-5)mol/L)).Sulfide electrode method was used to measure the content of hydrogen sulfide in supernatant and the production rate of hydrogen sulfide in homogenate.Results Compared with the control group,hydrogen sulfide in supernatant was significantly increased(P

Objective To discuss the methods of treating diabetes merged oral and maxillofacial space infec-tion and their effects.Methods Clinical data of 20 patients with diabetes merged oral and maxillofacial space infec-tion were retrosepectively analyzed.Results All 20 cases of patients with diabetes merged oral and maxillofacial space infection were cured.The mean length of treatment was (18.4 ±2.8)days.There were 20 cases of positive results of bacterial culture,the positive rate was 100%,and there were 15 cases of streptococcal infection,4 cases of klebsiella pneumoniae infection,1 case of candida albicans infection.20 cases of patients was given incision and drainage,4 cases given incision of trachea.Conclusion It was easier for patients with diabetes having maxillofacial space infection,and more serious illness,more difficult for treating.Local infection should be treated,and systemic conditions should be improved as well.The risks of occurrence of serious complications should be reduced.

Objective To evaluate the clinical efficacy and safety of valsartan and methylprednisolone com-bined with cyclophosphamide for treatment of IgA nephropathy with prevailing proteinuria.Methods 118 case of IgA nephropathy patients with prevailing proteinuria were divided into the research group(60 cases)and control group (58 cases)according to random number table.Patients in the control group were given valsartan capsules(80mg/d) and methylprednisolone tablets(1mg·kg -1 ·d -1 ),patients in the research group were given cyclophosphamide tab-lets on the basis of control group(0.5 ~1.0g/m2 ,1 time /month,a total of 10 times).All the patients were treated for 12 months.The clinical efficacy,24h urine protein quantitative and plasma -albumin(ALB)were observed before and after treatment.Results After treatment,the clinical efficacy of research group and control group were 79.33% and 43.10%,the data of research group was significantly higher than that of the control group(χ2 =19.401,P <0.01), the 24h urine protein quantitative of the research group was significantly decreased from (2.0 ±1.3)g/24h before treatment to (0.9 ±0.2)g/24h in the 12 month after treatment(t =7.184,P <0.01),the ALB of the research group was significantly increased from (32.2 ±4.2)g/L before treatment to (39.5 ±4.8)g/L in the 12 month after treat-ment(t =5.335,P <0.01),the 24h urine protein quantitative of the research group afer 4,6,8,10,12 month were significantly lower than those in the control group(t =3.461,3.854,4.021,3.976,3.365,all P <0.05),the ALB of research group afer 8,10,12 month were significantly higher than those in control group(t =3.850,5.020,5.560,all P <0.05).Conclusion Valsartan combined methylprednisolone can more effectively reduce urinary protein and pro-tect renal function for the treatment of IgA nephropathy.

Objective To explore the application effect of performance evaluation in bonus distribution in nurses working in otolaryngology department,and seek for the perfect solution of bonus distribution,motivate nurses' work enthusiasm,and therefore be helpful for the enhanced nursing quality andsafety.Methods Bonus coefficient was calculated according to technical title (make up 40％),position(make up 30％) and performance assessment(make up 30％).The occurrence of nursing defects,patients satisfaction degree,the scores of nursing performance,the scores of theoretical test and skill examination,and nurse satisfaction degree were compared between pre-and post-implementation of system.Results The above items showed significant differences before and after the practice of performance evaluation.Conclusions Bonus distribution according to performance evaluation can be effective in the improvement of nursing quality,patients' satisfaction degree and nurses' work enthusiasm,therefore be helpful for the enhanced nursing management level in otolaryngology department.

Objective To investigate the curative effect and mechanism of mosapride and Shugan Jieyu capsule in the treatment of functional dyspepsia(FD).Methods One hundred and ten cases patients with FD were divided into two groups according to the digital random table method,with 55 cases in each group.The control group took mosapride tablets orally(5 mg/times,3 times/d,30 min before meals),the treatment group took with Shugan Jieyu capsule orally(2 pellets/times,2 times/d,orally with warm water on morning and evening)on the basis of treatment of the control group.Two groups were treated continuously for 4 weeks.The symptoms score and total effective before and after treatment were compared,and plasma Leptin(Leptin),Ghrelin and gastric dynamic element(MTL)levels of the two groups before and after treatment were compared.The two groups were followed up for 6 months after treatment,the recurrence rate of the two groups was compared.Results The symptoms integral of the treatment group after treatment was lower than that of the control group((4.97±1.85)points vs.(7.35±2.28)points,t=6.011,P＜0.01),the total effective rate was higher than that of the control group(94.5％(52/55)vs.81.8％(45/55),x2=4.274,P=0.039),the improvement of plasma Leptin((13.10±2.07)μg/L vs.(14.66±2.11)μg/L,t=3.914,P=0.001),Ghrelin((3.52±0.70)ng/L vs.(2.95±0.67)ng/L,t=4.363,P＜0.01),MTL((281.47±61.09)ng/L vs.(242.31±65.28)ng/L,t=3.248,P=0.002)levels were better than those of the control group,the recurrence rate of 6 months was lower than that of the control group(7.5％(5/53)vs.24.4％(11/45),x2=4.124,P=0.041),the difference was statistically significant.Conclusion Mosapride tablets combined with Shugan Jieyu capsule can benefit the liver symptom,and also can reduce the recurrence rate,it has curative effect on FD,its mechanism may be related to the improvement of BGP levels disorder in patients with FD.

Objective To investigate the effects of puerarin on renal advanced glycosylation end products (AGEs), expression of transforming growth factor-?1 (TGF-?1) and pathomorphism in diabetic rats. Methods Rats were randomly divided into following groups:normal control rats (Group CN), diabetic rats (Group DM) and diabetic rats treated with puerarin (Group DP). After Puerarin was given to STZ-induced diabetic rats for 10 weeks, blood glucose, urine protein, glomerular basement membrance thickness (GBMT) and profile of kidney hypertrophy in diabetic rats were measured and analysed. The renal pathologic changes were observed by light microscopy and electron microscopy. Renal AGEs content was determined by expose time of fluorescence microscopy. The expression of TGF-?1 was examined by immunohistochemical methods. Results Urine protein, GBMT and profile of kidney hypertrophy were significantly increased in diabetic rats as compared with control animals (P

Objective To explore the protective effect of herbal medicine to tonify qi and strengthen the spleen on liver damage induced by antituberculotic according to the TCM theory "reinforce the earth to reduce the wood".Methods The 100 tuberculosis patients randomized into two groups,50 in each,all took antituberculosis drugs with the liver-protecting medicine,Tiopronin,and the herbal medicines to nourish qi and strengthen the spleen were administered to those in the treatment group.Incidence of liver damage and the changes of liver function including serum alanine aminotransferase(ALT),aspartate aminotransferase(AST) and total bilirubin(TBiL) were observed for four weeks.Results In the treatment group,the incidence of liver damage was significantly lower than that in the control group(P

Objective:To observe and investigate the effects of real-time dynamic glucose monitoring, continuous subcutaneous insulin infusion combined with Carelink management software analysis on blood glucose control in newly diagnosed type 2 diabetic patients.Methods: 64 cases of patients with newly diagnosed with type 2 diabetes were selected from our hospital, and randomly divided into observation group and control group. The observation group were treated by real-time dynamic blood sugar monitoring and continuous subcutaneous insulin infusion joint Carelink management software analysis system, while the control group by continuous subcutaneous insulin infusion in combination with the real-time dynamic blood sugar monitoring. We compared serum C peptide, the rise of serum insulin levels and the decrease of blood glucose after ten days of treatment. At the same time, insulin resistance index (HOMA - IRI) and hypoglycemic events were compared between the two groups.Results: After treatment, the decrease range of blood glucose, postprandial blood glucose and HOMA-IRI in the observation group were significantly better than those in the control group (t=4.362,t=3.254,t=4.289; P<0.05). At the same time, the increase range of elevated fasting insulin, postprandial insulin, fasting C peptide and sugar after C peptide in the observation group were significantly better than those in the control group (t=3.712,t=4.115,t=3.831,t=3.889;P<0.05). The standard number of days of the blood glucose in the control group were significantly higher than those in the observation group (t=4.583,P<0.05).Conclusion: For patients with type 2 diabetes treated by real-time continuous glucose monitoring and continuous treatment of infusion combined with subcutaneous insulin Carelink management software can effectively reduce blood glucose, improve the insulin resistance of patients, reduce hypoglycemia and increased insulin sensitivity, meanwhile also can better protect the islet beta cell function.