Objective To establish a method for the determination of Ca2 + cations in the nutritive food by ion chromatography.Methods After being digested by the mixture of nitric acid and perchloric acid,the Ca2+ cations in the nutritive food were determined by ion chromatography(IC).Results The optimum chromatographic conditions were studied thoroughly,the linear range was 0.10-500 mg/L,r=0.9998.The lowest detection limit of Ca2+ cations in nutritive food was 0.02 mg/L,RSDs were 0.50%-4.00%,the rates of recovery tested by the standard addition method were in the range of 95.0%-103.0%.Conclusion This method is simple,fast,sensitive and accurate.

BACKGROUND: Balance disorder of the two neurotransmitters-dopamine and glutamic acid in striatum, participates in the pathogenic process of Parkinson disease. Whether or not electroacupuncutre can regulate the content of glutamic acid in striatum in the treatment of Parkinson disease so as to recover the balance of the two neurotransmitters is needed further investigation.OBJECTIVE: To observe the changes of content of excitatory amino acid (glutamic acid) in striatum of rat with Parkinson disease and the effects of electroacupuncture intervention on these changes.DESIGN: Randomized controlled trial.SETTING: Teaching and Research Section of Acupuncture and Moxibustion, Hubei University of Traditional Chinese Medicine.MATERIALS: The experiment was completed in Laboratory for Important Subject (Acupuncture and Moxibustion) of Hubei University of Traditional Chinese Medicine from February to August 2002. A total of 40 healthy SD rats were selected and randomly divided into 4 groups, namely, normal group, model group, sham operation group and electroacupuncutre group with 10 in each group.METHODS: Model group: Rotation model of rat with Parkinson disease was established by method of chemical damage on right substantia nigra.Sham operation group: Method of sham operation was the same as model group, and 3.4 μL physiological saline was injected. Electroacupuncture group: Electroacupuncture treatment was given after model establishment;took Atlas of Experimental Animal Acupoints as reference, selected the acupoints of Taichong and Fengfu, pierced with needles which were connected with G6805 electroacupuncture therapeutic apparatus under the condition of continuous waves and frequency of 2 Hz for 30 minutes, once a day for consecutive 7 days, then detected the content of glutamic acid.MAIN OUTCOME MEASURES: Content of glutamic acid in bilateral striatum of rat in normal group, sham operation group, model group and electroacupuncture group, which was detected by automatic amino acid analyzer.RESULTS: The content of glutamic acid in right striatum of rat in model group was significantly higher than that in contralateral striatum, normal group and sham operation group [the contents of glutamic acid in model group, contralateral striatum, normal group and sham operation group were (101.6±7.49), (71.4±5.65), (61.8±4.38), (62.6±5.21) μg/g respectively,P ＜ 0.01]. The content of glutamic acid in right striatum in electroacupuncture group was significantly lower than that in model group [the contents of glutamic acid in electroacupuncture group and model group were (83.7±5.68), (101.6±7.49) μg/g respectively, P ＜ 0.05], but still higher than that in normal group and sham operation group (P ＜ 0.05). There was no significant difference between the contents of glutamic acid in left striatum in each group (P ＞ 0.05).CONCLUSION: Excitatory amino acid participates in the pathogenic process of Parkinson disease. Therefore, one of the approaches of electroacupuncture treatnent for Parkinsonism is reducing the release of excitatory amino acid.

OBJECTIVE: To investigate laryngopharyngeal reflux effect on expression of COX-2mRNA in glottis carcinoma lesion mucosa. METHOD: Forty cases with glottic laryngeal cancer were examined by electronic naspharyngeal laryngoscope and scored by the reflux symptom index (RSI) and the reflux finding score(RFS). Based on the scores, they were divided into two groups:glottic laryngeal cancer with positive reflux(20 cases) and glottic laryn geal cancer with negative reflux (20 cases). Ten cases with adjacent normal membrane were used as control group. The mRNA expression of CoX-2 from 40 patients was examined by reverse transcription polymerase chain reaction (RT-PCR). RESULT: The expression of COX 2mRNA in tumor samples was significantly higher than that in normal tissues (P < 0.05); the expression of COX-2mRNA in glottic laryngeal cancer with positive reflux was significantly higher than that in glottic laryngeal cancer with negative reflux (P < 0.05). CONCLUSION Laryngopharyngeal reflux factors may increased expression glottic carcinoma of COX-2mRNA by tissue injury, inflammation and cell malig-
Cyclooxygenase 2 ; genetics ; metabolism ; Female ; Glottis ; Humans ; Laryngeal Neoplasms ; complications ; metabolism ; Laryngopharyngeal Reflux ; complications ; metabolism ; Male ; Mucous Membrane ; metabolism ; RNA, Messenger ; metabolism ; Reverse Transcriptase Polymerase Chain Reaction

Objective:To investigate spinal cord neurons fos,heat shock protein 70(HSP70)change and influence of Danshen Injection after the rats’limb ischemia reperfusion.Methods:Through temporarily interdict the rat’s one side iliac artery and femoral artery to establish the model of the limb ischemia reperfusion injury.Using the immunohistochemical method of ABC to observe the expressions about neurons fos and HSP70 positive neurons and the character about their distributions,as well as their change after being intervened by Danshen Injection n.Results:The rats’spinal cord neurons fos and HSP70 expressions increased in four hours later the limb ischemia(P

Objective To investigate the changes in atrial tissue and atrial myocytes as a result of chronic atrial fibrillation (AF) in humans. Methods Twenty patients with rheumatic heart disease were divided into chronic atrial fibrillation group (n=10) and non-fibrillation group (n=10). Tissue specimens harvested from the atrial appendage were studied with light and electron microscopy. Results The content of atrial connective tissue in the fibrillation group (26.7?7.2) was significantly higher than that in non-fibrillation group (12.4?5.9) (P

Objective To study the relationship between intracellular calcium concentration of atrial myocytes and atrial fibrillation (AF) in humans. Methods Atrial myocytes were isolated from right atrial appendages of rheumatic heart disease (RHD) patients with or without AF, and also isolated from that of congenital heart disease (CHD) patients with sinus rhythm. Intracellular Ca 2+ concentration was measured with the fluoresent Ca 2+ indicator Fluo-3 and laser scanning confocal microscopy. Results Intracellular Ca 2+ concentration of RHD patients with AF was significantly higher than that of non-AF RHD patients [(517?98) nmol/L vs (262?65) nmol/L, P

AIM: To investigate the anti-DIC effect of the domestic recombinant hirudin (rH) in order to provide preclinical pharmacological basis for its new drug application. METHODS: DIC was induced by constant iv infusion of thrombin (100 U?kg -1 ?h -1 ?1 h) plus PAMBA (50 mg?kg -1 ?h -1 ?1 h). Platelet count (PLT), PT and fibrinogen (FIB) determination as well as plasmaprotamine paracoagulation (3P) test served as diagnostic indexes of DIC, while pathlogical examination of microthrombosis of lungs and kidneys was followed. RESULTS: rH 125,62.5,31.25 and 15.625 ?g?kg -1 iv. gtt demonstrated a dose-dependant inhibitory effect on DIC. The inhibitory effects on DIC of rH(%) were 89.38%, 52.60%, 49.71% and 25.58% for PT, and 100.01% , 61.86%, 58.40% and 32.68% for PLT, and 85.22%, 73.01%, 33.60% and 14.86% for FIB respectively at the descending order of rH doses. CONCLUSION: Domestic rH being tested has powerful anti-DIC effect which is comparable with refludan—an r-hirudin product approved by European Community for marketing.

Objective To observe the effect of electroacupuncture (EA) on dopaminergic neurons in the mesencephalic substantia nigra(MSN) of rats with Parkinson′s disease (PD). Methods A model of PD was induced in rats by injecting 6-hydroxydopamine (6-OHDA) into the MSN. Acupuncture was then used to treat the model rats. To examine the apoptosis of dopaminergic neurons, the number of tyrosine hydroxylase (TH) positive neurons and the concentration of dopamine (DA) were measured in a normal control group、a sham-operation group、the EA group and the PD group which received no EA. Results In the EA group, markedly less apoptosis of dopaminergic neurons was observed, and the number of TH positive neurons and the concentration of DA were significantly higher. Conclusion Electroacupuncture may mitigate injury to the substantial nigra in Parkinson′s disease.

Objective To investigate the possible mechanism and re-ablation of recurrent atrial arrhythmias after atrial fibrillation(AF) ablation by utilizing the Carto system and double LASSO technique.Methods A total of 77 AF patients were enrolled and among them,56 with paroxysmal AF and 21 with persistent AF.Eight-F Swartz long sheaths were positioned in the left atrium by transseptal technique.One to two LASSO catheters were placed in the ipsilateral pulmonary veins(PV).The anatomy of the left atrium and PV was established using the Carto system.PV selective venography was carried out to identify the PV ostium.Continuous circular lesions(CCL) were made at the left atrium at 0.5 to 1.0 cm outside the PV ostium.Irrigated radiofrequecy energy was delivered in all the patients.The end point of the CCL was defined as absence of all PV spikes documented with LASSO catheters within the ipsilateral PVs.Results A repeat ablation was performed in 14 patients who experienced highly symptomatic atrial tachyarrhythmia.During the repeat procedures,conduction gaps in the previous CCL were found in 13 out of the 14 patients,and a typical atrial flutter was found in the other patient which was eliminated by ablation of the tricuspid isthmus.During the second procedure,pulmonary vein tachycardia was demonstrated in 7 patients.The pulmonary vein tachycardia activated the LA via the conduction gaps.All conduction gaps were successfully ablated by irrigated RF applications.After the second procedure,12 out of the 14 patients were free of AF during 3 to 30 months of follow-up.Conclusion Continuous circular lesions(CCL) in the left atrium utilizing the Carto system and double LASSO technique had a high success.In patients with recurrent atrial tachyarrhythmia after CCLs,recovered PV conduction is a main cause for the recurrence.

Objective To investigate the effect of taurine(Tau) on the myocardial fibrosis and its mechanism.Methods The rat model of myocardial fibrosis was built by subcutaneous injection of isoprel(Iso).And the rats were divided into control group,model group and taurine group(80,160,320 mg?kg-1?d-1).Hydroxyproline measurment was carried out for detecting collagen quantification in myocardium tissue.Histopathology changes were detected by HE staining.In vitro,the cultured neonatal rat cardiac fibroblasts(CFb) were divided into control group,model group and taurine group(40,80,160mmol?L-1).The proliferation of CFb was induced by angiotensin Ⅱ(AngⅡ) and detected by the thiazoleblue(MTT)colorimetric assay.TGF-?1 level was determined by ELISA technique.The expression of cyclin dependent kinase inhibitor p27 was assessed with flow cytometry.Results In vivo,the contents of hydroxyproline were decreased in Tau(160,320 mg?kg-1?d-1) groups as compared with model group(P