The method of combining culture of embryonic chicken mesonephrons or heart fragments with some cells on double layer agar-agar medium is used in this experiment. The authors have investigated with this method invading capacity of malignant esophageal cancer cell line (ECa-109), malignant hepatoma cell line (BEL-7402) and normal diploid Chinese hamster ovary cell line (CHO) to embryonic chicken mesonephrons and heart fragments.The authors have found that both ECa-109 cell line and BEL-7402 cell line have invasive capacity in vitro. The invasive capacity of ECa-109 cells is higher than that of BEL-7402 cells. The normal diploid CHO cells can not invade target organs and show only slight adhesion to them. The capacity for ECa-109 cells or BEL-7402 cells to invade both the embryonic chicken mesonephrons and heart fragment is similar.

Objective To investigate the effect of nerve growth factor(NGF)injection solution on immunosuppression in mice.Methods The immunosuppressed mice were induced by cyclophosphamide(Cy,80mg/kg,sc).The immunomodulatory effects of the drug were measured by cleaning carbon particle method,simple diffusion method,delayed type hypersensitivity(DTH),3H-TdR entering method and indirect immunofluorescence assay.Results The k value;a value,the level of serum IgG and ear swelling were remarkably decreased by NGF injection [0.01,0.1 mg/(kg? d)?10d,im].In addition,the number of L3T+4 Cells of peripheral blood and the ratio of L3T+4/Ly-2+ cell increase.Conclusion NGF has an enhanced effect on experimental immunosuppression in mice,which provides a theoretical basis for NGF treating tumor patients with nervous injure by chemotherapy or radiotherapy.

Objective To explore the effect of dendritic cells (DC) primed by total RNA extracted from human colon cancer Lovo cell on specific cytotoxicity of cytokine-induced killer cells (CIK) in vitro.Methods Cord blood mononuclear cells extracted by Ficoll density gradient centrifuge were induced into CIK and DC cells separately, and their Immunophenotype was detected by Flow cytometer. Trizol harvested total RNA from colon cancer cell Lovo and the RNAs were loaded to DCs obtained from cord blood as tumor anti gens. Effectors were grouped accordingly as CIK cells co-cultured with DCs transfected with Lovo RNA, CIK cells co- cultured with unloaded DCs and CIK cells. Targets was Lovo cells. In vitro cytotoxicity of CHK cells was extured with DCs loaded with Lovo RNA(76.49%±4.21%), DC + CIK group was lower(53.84% ± 2.15%),and CIK cells group possessed the lowest cytotoxicity(32.20% ± 3.07%), showing statistic significance( P ＜0.05). Conclusion Extraction of total RNA from tumor cells is simple and easy for clinical implementation.Total RNAs acted as antigen to pulse DCs can strengthen the specific cytotoxicity of CIK cells, which will have good prospects for clinical application.

OBJECTIVETo observe the effect of Ginkgo biloba extract (GbE) against the bleomycin induced pulmonary fibrosis and to investigate its mechanisms.

METHODSD rats were randomly divided into 3 groups: Control group, BLM + NS group and BLM + GbE group (n = 6 in each time point of each group). Rats in each group were sacrificed on the 14th and 30th day after endotracheal injection of bleomycin A5. Lung injury through HE stain and pulmonary fibrosis through Masson stain were observed by light microscope. The content of collagen protein in lung tissue and malondialdehyde (MDA) in plasma were assayed by biochemical methods. The expressions of TGF-beta1 in tissue and bronchoalveolar lavage fluid (BALF) were detected by immunohistochemistry and immunocytochemistry respectively.

RESULTCompared with control group, every datum in each time point in BLM + NS group showed significant changes which indicated the success of the model. Compared with BLM + NS (14 d) group, MDA in serum and TGF-beta1 in alveolar macrophage were significantly reduced in BLM + GbE (14 d) group. The data in BLM + GbE (30 d) group were compared with those in BLM + NS (30 d) group as follows. The lung injury and fibrosis were significantly ameliorated, the content of collagen in lung tissue and MDA in plasma were significantly reduced, the expression of TGF-beta1 in lung tissue was significantly reduced, however the expression of TGF-beta1 in BALF cells was not significantly changed.

CONCLUSIONGbE inhibited bleomycin induced lung injury and fibrosis in rats. The possible mechanisms were that GbE could inhibit the expression of TGF-beta1 in alveolar macrophage in early stage of fibrosis (14 d) and in noninflammatory cells in proliferative stage (30 d), and GbE could also attenuate the oxidative stress induced by bleomycin.

Animals ; Bleomycin ; adverse effects ; analogs & derivatives ; Bronchoalveolar Lavage Fluid ; chemistry ; Disease Models, Animal ; Drugs, Chinese Herbal ; administration & dosage ; Ginkgo biloba ; chemistry ; Humans ; Macrophages, Alveolar ; drug effects ; metabolism ; Male ; Malondialdehyde ; metabolism ; Pulmonary Fibrosis ; chemically induced ; drug therapy ; genetics ; metabolism ; Random Allocation ; Rats ; Rats, Sprague-Dawley ; Transforming Growth Factor beta1 ; genetics ; metabolism

Objective:Grasp the status of Zhejiang entry standards, controls and calibrators in recent years and problems.Methods:Through descriptive statistics, cross-tab chi-square, and comparation of means method , we collected and sorted out relevant information about standard products, quality control products and calibrators of the Zhejiang Provincial Biomedical Special Articles Entry Centralized Supervision Platform, and analyzed the basics of standard products, quality control products and calibrators situation, degree of dependence and utilization.Results:The standard products and quality control products imported into Zhejiang province are mainly used for scientific research of biomedical device enterprises. 76.6% of imported standard products, quality control products and calibrators cannot be domestically produced. The main origin of imported standard products and quality control products is the United States, and the main origin of standard products is the United Kingdom.Conclusions:The standard products, quality control products and calibration products imported into Zhejiang province are currently lacking in domestic production capacity. It is recommended to support the research and development and production of domestic standard products, quality control products and calibration products.

Patients with severe trauma require an extremely timely treatment and transfusion plays an irreplaceable role in the emergency treatment of such patients. An increasing number of evidence-based medicinal evidences and clinical practices suggest that patients with severe traumatic bleeding benefit from early transfusion of low-titer group O whole blood or hemostatic resuscitation with red blood cells, plasma and platelet of a balanced ratio. However, the current domestic mode of blood supply cannot fully meet the requirements of timely and effective blood transfusion for emergency treatment of patients with severe trauma in clinical practice. In order to solve the key problems in blood supply and blood transfusion strategies for emergency treatment of severe trauma, Branch of Clinical Transfusion Medicine of Chinese Medical Association, Group for Trauma Emergency Care and Multiple Injuries of Trauma Branch of Chinese Medical Association, Young Scholar Group of Disaster Medicine Branch of Chinese Medical Association organized domestic experts of blood transfusion medicine and trauma treatment to jointly formulate Chinese expert consensus on blood support mode and blood transfusion strategies for emergency treatment of severe trauma patients ( version 2024). Based on the evidence-based medical evidence and Delphi method of expert consultation and voting, 10 recommendations were put forward from two aspects of blood support mode and transfusion strategies, aiming to provide a reference for transfusion resuscitation in the emergency treatment of severe trauma and further improve the success rate of treatment of patients with severe trauma.