Objective:To evaluate the psychosomatic effects of relaxation and the correlation between the effects and the personality and behavior pattern of the subject.Methods:68 medical students were involved in the study. All the subjects received relaxation training for 2 months. They were tested with Self-Rating Anxiety Scale (SAS) and Pittsburgh Sleep Quality Index (PSQI) before and at the end of the study. They were also tested electroencephalogram (EEG), electromyography (EMG) and skin temperature at the above two time-points. The correlation between the personality and the extents of alteration in index of EEG were also studied.Results:After training, the scores of SAS and PSQI in the subjects were significantly lower than those before training. The indexes of EEG, EMG and skin temperature were improved. The extents of alteration in index of EEG were correlated positively with the N-scores of Eysenck Personality Questionnaire (EPQ), the total scores and TH-scores of Type A Behavior Pattern (TABP).Conclusion:Relaxation can improve psychosomatic states of undergraduates. The extents of alteration in index of EEG were correlated with the personality and behavior pattern of the subject.

BACKGROUND: The curative effect is satisfactory for adult patients with spasticity of lower limbs treated with selective posterior rhizotomy and peripheral nerve micro diminution. But how to improve the strength of relevant muscle is the key factor to accelerate recovery of motor function during rehabilitation training.OBJECTIVE: To observe the effect of muscle stimulating instrument on the recovery of muscle strength and the improvement of motor function of adults with spasticity of lower limbs during rehabilitation training.DESIGN: Case analysis.SETTING: Department of Neurosurgery, China-Japan Friendship Hospital of Beijing Ministry of Public Health.PARTICIPANTS: Totally 49 adults with spasticity of lower limbs were selected from Department of Neurosurgery, China-Japan Friendship Hospital of Beijing Ministry of Public Health from January 2000 to May 2002.There were 37 males and 12 females aged from 19-48 years. Totally 21patients treated with muscle stimulating instrument were determined as treatment group and other 28 patients were determined as control group during rehabilitation training.METHODS: One day after operation, conventional rehabilitation training was performed on patients in the treatment group and the control group.Patients in the treatment group were also treated with muscle stimulating instrument three times a day with each for 30 minutes for totally 7 days as a course. There was a three-day interval between treating courses and the rehabilitative time lasted for 6 months. Before rehabilitation training, indexes of patients in the two groups, such as ankle extension, knee flexion and muscle strength of thigh adductor, were recorded and the improvement of muscle strength after 3 and 6 months was followed up.MAIN OUTCOME MEASURES: Average strength of relevant muscle before and after 3-month and 6-month rehabilitation training.RESULTS:Totally 49 patients entered the final analysis.①After 3-month and 6-month treatment, indexes of ankle extension, knee flexion and muscle strength of thigh adductor were increased at various degrees.②During 3-month and 6-month treatment,indexes of ankle extension,knee flexion and muscle strength of thigh adductor in the treatment group were obviously higher than those in the control group [3-month treatment:(4.2±0.8), (3.7±0.7) degrees; (4.3±0.7), (3.8±0.7) degrees; (4.0±0.7), (3.5±0.5)degrees; 6-month treatment: (4.5±0.6), (3.9±0.7) degrees; (4.6±0.7), (4.0±0.5)degrees; (4.4±0.7), (4.0±0.6) degrees, (P ＜ 0.05 or P ＜ 0.01)].CONCLUSION: The combination of rehabilitation training and muscle stimulating instrument can accelerate the recovery of muscle strength and motor function in adults with spasticity of lower limbs after microsurgical treatment.

ObjectivesTo evaluate the therapeutic result of various management for severe radiation injury of the bowels.MethodsThe clinical presentation, diagnostic approach, managements and therapeutic effects were analysed retrospectively in 28 severe radiation therapy caused enteric injury patients. In all intestinal stricture cases, partial ileal resection was performed in 14 patients, right hemicolectomy in 1 patient, rectosigmoid resection in 3 patients. Instestinal fistulae was treated by diseased segment resection and reanastomosis in 7 patients, sigmoidostomy was undertaken in 2 patients.ResultsThere was no inhospital mortality. One case suffered from low colonic fistula,4 died of recurrence of the primary tumor.Postoperative bowel function and nutrition status significantly improved in all patients.ConclusionSurgical management combined with nutrition support is safe and effective for the treatment of severe radiation caused enteric injury.

Objective To observe the effect of Huahongjiangxiantang on hemorheology and plasma fibrinogen (FIB).Methods 100 patients with hypertension were randomly divided into two groups:the treatment group ( n=50) which were treated with Huahongjiangxian-tang,and the control group( n=50)were treated with Aspirin.Hemorheology and plasma fibrin were detected before and after treatment for 8 weeks.Results After 8 weeks treatment,there were significant depressions in whole blood viscosity(low shear rate),RBC deformability index and RBC aggregation index( P＜0.01 ),and better than the control group( P ＜0.05 ).There were significant changes in whole blood viscosity ( high shear rate),plasma viscosity,RBC rigidity index and plasma fibrin ( P ＜ 0.05 ).A number of indicators declined significantly in control group ( P ＜ 0.05 );The rate of platelet aggregation to improve the situation in the control group was better than that of the treatment group,but there was no statistical significance.Conclusion Huahongjiangxiantang can effectively reduce blood viscosity in patients with hypertension and improve the body's red blood cell function and fibrinolysis,benefit to the prevention and treatment of atherosclerosis and reduce the occurrence of thrombotic events.

Objective To investigate the effect of ketamine on the expression of HSP 70 in myocardium in severely burned rats for its possible mechanism of myocardial protection. Methods Seventy-two male Wistar rats were randomly divided into 3 groups: normal control group (group C, n=8), burn injury group (group BI, n= 32) and ketamine group (group K,n=32). 30% Wtal body surface area of Ⅲ degree burn model was developed in group BI and group K. Ketamine 20 mg/kg was injected IM in group K 15 min after the burn model was made. Equal volume of normal saline was given instead of ketamine in group BI. Group C received no treatment. The rats were sacrificed at 3, 6, 12 and 24 h after administration in group BI and group K respectively(8 rats at each time point). Myocardial samples were obtained for determination of the expression of HSP 70 by Western blot analysis. The myocardial ultrastructure was observed at 3 h after administration with electron microscope. Results The myocardial damage was milder in group K than in group BI. The expression of HSP 70 was significantly higher at 3, 6, 12 and 24 h after administration in group K and group BI than in group C(P<0.05).The HSP 70 expression was significantly higher at 3 and 6 h after administration in group K than in group BI ( P<0.05). Conclusion Ketamine can reduce the myocardial injury induced by severe burn through up-regulating the expression of HSP 70 in cardiocytes.

OBJECTIVE：Probe into the feasibility of preparation of diclofenac sodium with β-cyclodextrins in 1∶1 and 2∶1 inclusion compounds ,and find out the affection in solubility of diclofenac sodium. METHODS:The 1∶1 and 2∶1 melusion compounds were prepared by liquid-phase an alysis the content and determination them solubility.RESULT:The 1 ∶1 and 2∶1 inclusion compounds were prepared by different ratio of reactants and etermined them mp were 299～300.5℃ and 302～303℃ respectively. CONCLUSION：Of us is that solubilits of the 1∶1 and 2∶1 inclusion compounds increase 0.65 and 1 times respetively comparing with declofenac sodium.

Objective: To establish the standard for the quality control of Dougenguanshitong granule.Methods: The TCL method was used to identify the Radix Ootoginseng and Dioscorea Bulbifera in the granule and the content of matrine in the granule was determined by the HPLC method.Results: The linear relationship of matrine was the range of 0.68-10.2?g(r=0.9997,n=6).The average recovery was 100.23% and RSD was 1.54%(n=6).Conclusion:The method is convenient,rapid,accurate and suitable for the quality control of Dougenguanshitong granule.

In recent years, the Gut Microbiota-Brain Axis theory has pointed out that there is an interaction between gut microbiota and central nervous system diseases.A number of studies have shown that there is a link between gut microbiota and depression, Parkinson's disease, and Alzheimer's disease.The association between gut microbiota and stroke has also attracted wide attention.This article reviews the association between gut microbiota and stroke.

To identity the pathogen that causes the mosaic and yellowing symptoms on Atractylodes macrocephala Koidz in Jiangxian, Shanxi province, biological inoculation, sequence-independent amplification (SIA),RT-PCR and other identification methods were used. The results showed that the chlorotic and necrosis symptoms occurred in the indicator plant Chenopodium quinoa after it was infected with the pathogen,and the same symptoms appeared after the reinoculation of healthy Atractylodes macrocephala Koidz; this reflected that the disease was likely to be caused by a virus. The results of SIA and sequencing showed that Broad bean wilt virus 2 (BBWV2) was present in severely mosaic Atractylodes macrocephala Koidz leaves. To further characterize the BBWV2 isolate from Atractylodes macrocephala (BBWV2-Am), the polyprotein partial gene encoded by BBWV2-Am RNA2 was cloned and sequenced. Sequence alignments showed that the nucleotide sequence identity of BBWV2-Am SCP and LCP genes ranged from 79.3% to 87.2% and from 80.1% to 89.2% compared to other BBWV2 strains,respectively; the deduced amino acid sequence similarities of the two gene products ranged from 91.2% to 95.7% and from 89.44 to 95.5%, respectively,compared to those of other BBWV2 strains. Phylogenetic comparisons showed that BBWV2-Am was most likely to be related to BBWV2-Rg,but formed an independent branch. This is the first report of BBWV2 in Atractylodes macrocephala Koidz.
Amino Acid Sequence ; Atractylodes ; virology ; Fabavirus ; chemistry ; classification ; genetics ; isolation & purification ; Molecular Sequence Data ; Phylogeny ; Plant Diseases ; virology ; Sequence Analysis ; Viral Proteins ; chemistry ; genetics

Objective To explore the establishment method of brain ischemia model with blood stasis. Methods The rat models of blood stasis were established by intramuscular injection of dexamethasone 0.2 mg?kg-1?d-1 inside the thigh for successive 10 d. When the blood stasis models were successfully established, the rats were anaesthetized and common carotid arteries in both sides were separated and ligated to make brain ischemia model. Meanwhile the blank control group and Sham-operation to one of the model groups were conducted. Results Compared with the blank control group, the level of whole blood viscosity were obviously increased and TT、PT、APTT markedly decreased in the operation group and pseudo-operation group. Compared with the blank control group and the operation group, the changes of ATP activity,the LD ,LDH ,NO,NOS, SOD,MDA, Glu, T-AA, ET and CGRP levels were significant in the pseudo operation group(P