To explore The clinical value of Multimode ultrasound in evaluating cerebral hemorrhage with intracranial pressure（ICP）. Methods A total of 17 patients with cerebral hemorrhage who received lumbar puncture according to their medical necessity in the ICU of the Affiliated Hospital of Yanbian University from September 2019 to June 2021 were enrolled. The diameter of optic nerve sheath （ONSD） and transcranial Doppler ultrasound （TCD） were performed before lumbar puncture. The patients were divided into elevated intracranial pressure group （9 cases） and normal intracranial pressure group （8 cases），according to the results of lumbar puncture pressure （more than 200 mmH2O was defined as elevated intracranial pressure，and 80～200 mmH2O was defined as normal intracranial pressure）. The Systolic blood pressure，diastolic blood pressure，partial pressure of carbon dioxide，GCS，ONSD and TCD parameters （such as peak systolic velocity，end diastolic velocity，mean blood flow velocity and pulse index of bilateral middle cerebral artery） were compared between the two groups，and the correlation between ICP and ONSD，pulse index（PI） was analyzed. Results （1）The systolic blood pressure，diastolic blood pressure，partial pressure of carbon dioxide （P CO2） and GCS scores between the two groups were not significantly different （all P> 0.05）；（2）The ONSD was significantly higher in the elevated intracranial pressure group［（5.15±0.24） mm vs. （3.97±0.22） mm，t=10.69，P<0.001）］；（3）The systolic peak flow velocity （PSV），end diastolic flow velocity （EDV） and mean flow velocity （MV） between the two groups were not significantly different（all P> 0.05），while the PI was significantly higher in the elevated intracranial pressure group［Right（1.20±0.19） vs.（0.95±0.12），t=3.148，P=0.007）；Left（1.20±0.17） vs. （0.92±0.10），t=3.893，P=0.001）］.（4）ICP was significantly associated with PI （r=0.52，P<0.02） and ONSD（r=0.64，P<0.01）. Conclusion Combine with Ultrasonographic ONSD measurement and TCD can effectively assess intracranial hypertension in patients with intracerebral hemorrhage.

Objective:To discuss the clinical efficacy of Qingfei Xiegantang on chronic inflammation and endothelial function of people of Taiyin constitution with metabolic syndrome （MS）. Method:Patients （162 cases） were divided into control group （80 cases） and observation group （82 cases）. Both groups got lifestyle intervention and treatment with lipid regulation， blood pressure reduction and hypoglycemia according to MS. Patients in observation group got Qingfei Xiegantang， 1 dose/day. Patients in control group got placebo granules of Qingfei Xiegantang. The treatment lasted for 4 months. Before and after treatment， weight， height， waist （WC）， hip， body mass index （BMI） and waist hip ratio （WHR） were calculated. Levels of triglyceride （TG）， total cholesterol （TC）， high-density lipoprotein cholesterol （HDL-C）， low-density lipoprotein cholesterol （LDL-C）， fasting blood glucose （FBG）， 2-hour postprandial blood glucose （2 hPG）， glycosylated hemoglobin （HbA1c） and fasting insulin （FINS） were measured. Insulin resistance index （HOMA-IR）， insulin sensitivity index （ISI） and islet beta cell function index （HOMA-β）， systolic blood pressure （SBD）， diastolic blood pressure （DBP）， tumor necrosis factor-α （TNF-α）， interleukin-6 （IL-6）， leptin （LP）， adiponectin （ADP）， nitric oxide （NO）， endothelin-1 （ET-1）， endothelial nitric oxide synthase （eNOS） and inducible nitric oxide synthase （iNOS） were detected and recorded. Then the safety was evaluated. Result:Levels of body mass， BMI， WHR， TG， TC， LDL-C， FBG， 2 hPG， HbA1c， HOMA-IR， SBD， DBP， TNF-α， IL-6， LP， ET-1 and iNOS were all lower than those in control group. Levels of HDL-C， InISI， HOMA-β， ADP， NO and eNOS were higher than those in control group （P<0.01）. And score of syndrome differentiation of Taiyin people was lower than that in control group （P<0.01）. The compliance rate of BMI in observation group was 70.27% （52/74）， which was higher than 53.42% （39/73） in control group （χ²=4.421， P<0.05）. The compliance rate of blood pressure was 95.95% （71/74）， was higher than 84.93% （62/73） in control group （χ²=5.171， P<0.05）. The compliance rate of blood fat was 87.84% （65/74）， which was higher than 72.60% （53/73） （χ²=5.386， P<0.05）. Conclusion:Qingfei Xiegantang can regulate the obesity， blood pressure， blood glucose and blood lipid components of MS， relieve clinical symptoms， improve IR， insulin sensitivity and islet β cell function， reduce inflammatory reaction， and increase vascular endothelial function of people of taiyin constitution with metabolic Syndrome.

OBJECTIVE: To explore the protective effect of Radix Rhapontici water extract (RRWE) on the damage of vascular endothelial cells induced by tert-butyl hydroperoxide (TBHP) in vitro. METHODS: The cellular model was established by treating human umbilical vein endothelial cells with TBHP, and randomly assigned to 4 groups:the control, TBHP, low and high-dose RRWE groups. Cell viability was tested by MTT assay, the levels of reduced glutathione (GSH), malondialdehyde (MDA), and superoxide dismutase (SOD) were measured by colorimetric method, the reactive oxygen species (ROS), apoptosis, and mitochondrial membrane potentials were observed by fluorescent staining, and the protein expressions of NF-κB, JNK, Bax, Bcl-2 and caspase-3 were determined with Western blotting method. RESULTS: Pretreatment with RRWE significantly increased the cell viabilities, reduced ROS levels, decreased MDA formation, increased the GSH contents and SOD activities, elevated the mitochondrial membrane potentials, down-regulated the p-JNK and p-NF-κB levels, reduced Bax/Bcl-2 ratios, suppressed caspase-3 activation, and inhibited cell apoptosis of vascular endothelial cells. CONCLUSION: RRWE has a protective effect on the damage of vascular endothelial cells induced by TBHP in vitro, and suppresses the cell apoptosis maybe through inhibiting JNK and NF-κB activation.

In recent years, nanoparticle as drug carriers have been widely used in the pharmaceutical field. For some poorly soluble drugs or drugs with strong toxicity and side effects, the design of different nano drug carriers can help achieve improved solubility and targeted therapy. The nano targeted drug delivery system can deliver drug specifically to the target sites and realize controlled release of drugs, thereby increasing the bioavailability and efficacy of drugs and reducing the toxicity and side effects. In this paper, the recent progress in studies on the design of nanoparticles, their biological effects, and their application in the field of medicine are reviewed.

OBJECTIVE: To investigate the effects of Rhaponticum uniflorum extract (RUE) on angiogenesis and apoptosis of H22 transplanted tumor tissue in mice. METHODS: Mice bearing H22 hepatoma cells were randomly assigned into 5 groups: model, high, medium and low dose RUE, and 5-fluorouracil (5-Fu) group. The intervention was lasted for 10 d. The pathological changes were detected with hematoxylin & eosin (HE) staining, the apoptosis of hepatoma cells were determined by DNA laddering method, the microvessel densities (MVD) were detected using immunohistochemical assay, and the protein expression of vascular endothelial growth factor (VEGF), vascular endothelial growth factor receptor 2 (VEGFR2) and hypoxia-inducible factor-1α (HIF-1α) were detected by Western blot method. RESULTS: RUE treatment reduced the cell proliferation, aggravated the necrosis of transplanted tumor tissue, reduced DNA fragmentation of H22 hepatoma cells, decreased MVD of tumor tissue, and down-regulated the protein expression of VEGF, VEGFR2 and HIF-1α of the transplanted tumor tissue, as compared with the model group. CONCLUSION: RUE could exhibit anti-angiogenic and pro-apoptotic effects against H22 hepatoma cells in mice, and its anti-angiogenic mechanism is probably related to down-regulation of VEGF, VEGFR2 and HIF-1α proteins.

OBJECTIVE: To investigate the protective effect of different solvent fractions of Boschniakia rossica on acute liver injury induced by acetaminophen (APAP) in mice. METHODS: The mice were randomly assigned to the normal control, model control, bifendate (positive control), as well as groups of different solvent fractions of Boschniakia rossica. Animals were treated once daily for 7d. APAP were given intraperitoneally to the mice of groups, then the alanine aminotransferase (ALT), aspartate aminotransferase (AST), albumin (ALB), reactive oxygen species (ROS), lipid hydroperoxide (LPO), malondialdehyde (MDA), glutathione (GSH), catalase (CAT), glutathione peroxidase (GSH-Px), superoxide dismutase (SOD), Na+-K+-ATPase, Ca2+-Mg2+-ATPase nitric oxide (NO) and inducible nitric oxide synthase (iNOS) were detected by the colorimetric method. RESULTS: The administration with butanol soluble fraction and aqueous fraction oi Boschniakia rossica reduced the serum ALT and AST activities, increased the scrum ALB level, decreased the hepatic ROS, LPO and MDA levels, increased the CAT, GPx, SOD activities and GSH level, increased the Na+-K+-ATPase and Ca2+-Mg2+-ATPase activities of liver mitochondria, and decreased hepatic iNOS activity and NO level of liver in mice with acute liver injury. CONCLUSION: The different solvent fractions of Boschniakia rossica have protective effects on acute liver injury induced by APAP in mice, probably via anti-oxidative and anti-inflammatory activities.

OBJECTIVE: To study the chemical constituents in the stems of Acanthopanax senticosus from Changbai Mountain area.

OBJECTIVE: To investigate the effect of Rhaponticum uniflorum water extract (RUWE) on the oxidative stress and DNA damage of liver with an acute liver injury induced by carbon tetrachloride (CCl4) in mice. METHODS: Mice were randomly assigned to the normal control, model, bifendate (BFD), as well as high and low dose groups of RUWE. Animals were treated once daily for 7 d. At the end of the experiment, CCl4 was given intraperitoneally to the mice of groups, then the alanine aminotransferase (ALT), aspartate aminotransferase (AST), lipid hydroperoxide (LOOH), malondialdehyde (MDA), glutathione (GSH), catalase (CAT), glutathione peroxidase (GSH-Px), superoxide dismutase (SOD), Na+-K+-ATPase and Ca2+-Mg2+-ATPase were detected with the colorimetric method, and DNA damage was analyzed using the electrophoretic method. RESULTS: The administration with RUWE reduced the serum activities of ALT and AST, decreased the LOOH and MDA contents of liver, increased the CAT, GSH-Px, total SOD, Mn-SOD activities and GSH level of liver, increased the Na+-K+-ATPase and Ca2+-Mg2+-ATPase activities of liver mitochondria, and reduced the DNA damage of hepatocyte in mice with acute liver injury. CONCLUSION: RUWE has protective effect on acute liver injury induced by CCl4 in mice, probably via reduction of the oxidative stress and DNA damage of liver tissue.

Objective: To explore the active constituents from the stems of Orostachys malacophyllus in Changbai Mountain. Methods: The ethyl acetate fraction of the methanol extract from the stems of O. malacophyllus was separated by chromatographic methods, structure identification was by means of nuclear magnetic resonance and mass spectra, and the activity was determined by CCK-8 experiment. Results: Five compounds were isolated and identified as 1-ethyl-2-hydroxysuccinate (1), 1-ethyl-2-O-[(3,4-dihydroxybenzoyl) oxy]-succinate (2), 1,4-dimethy-2-O-[(3,4-dihydroxybenzoyl) oxy]-succinate (3), 1,4-dimethy-2-O-[(4-dihydroxybenzoyl) oxy]-succinate (4), and diisobutyl phthalate (5). Conclusion: Compounds 1-5 are isolated from the plants of this genus for the first time. Among them, compound 2 is a new compound named orostachysolic acid, and exhibits cytotoxicity against HeLa cell with IC50 value of 111.5 μmol/L.

Objective: To investigate the effect of cold stress on the three gene families (PgHMGR, PgSSs, and PgSEs) of the ginsenoside biosynthetic pathway, and to explore the mechanism of genes response to cold stress and find the key genes. Methods: The cold stress treatment was performed by selecting 3 weeks fresh callus which placed in the 5℃ refrigerator, and harvested after 0, 0.5, 1, 2, and 3 d treatment for further analysis, were recorded as CK, D1, D2, D3, and D4, respectively. Results: The expression of PgHMGR1 reached 1.3 times compared to the control group at D3 period, and the expression of PgHMGR2 reached the peak at D1 period, which was 3.8 times of the control group; the expression of PgSS1 was 1.7 times of the control group (D3 period); and the expressions of PgSE2 and PgSE1 were 6.9 and 6 times higher than those of the control group, respectively. There was no significant change of the expression of PgSS2, PgSE3, and PgHMGR3.Conclusion: The gene families of ginsenoside biosynthesis pathway positively response to cold stress treatment, and PgHMGR1, PgHMGR2, PgSS1, PgSE1, and PgSE2 may be the key family genes when Panax ginseng callus response to cold stress.