In recent years,food intolerance has gradually become the focus of public attention.Food intolerance is a complicated allergic disease,which is different from food allergy.It may lead to systemic diseases.Because of its hidden and no specific symptoms,the diagnosis is often difficult.Through the detection of food specific IgG antibody,no intake or less intolerance food intake,can effectively improve the patient's symptoms,control and prevention of disease.This article reviews food intolerance from six aspects including its pathogenesis,the difference of food allergy,morbidity,the relationship with pediatric headache,the evaluation of diagnostic technique and nutrient adjustment.

Background and purpose:ERp29 belongs to endoplasmic reticulum stress proteins and might play roles in neoplasm metastasis. This study aimed to investigate the expression of ERp29 in Hca-F and Hca-P cells and to elucidate its role in lymphatic metastasis.Methods:Immunohistochemistry, Western blot and lfow cytometry analysis were used to detect the expression of ERp29 in Hca-F and Hca-P.Results:The results of immunohistochemistry suggested that ERp29 protein was located at cytoplasm of hepatic cells and some were also detected in the nucleus. The results of western blot suggested that ERp29 had positive expression in Hca-F and Hca-P cells. Its expression in Hca-F cells was apparently lower than that in Hca-P cells. And there was statically different between Hca-F and Hca-P cells (P<0.01). The relative lfuorescence intensity of ERp29 protein was signiifcantly lower in Hca-F cells (375.27±47.33) than that in Hca-F cells (623.91±46.80) by lfow cytometry (P<0.01).Conclusion:The different expression of ERp29 may be related to the potential ability of tumor lymphatic metastasis in Hca-F and Hca-P cells.

Health insurance is an important part of social security, and many problems have arisen in the con-struction of its legal system, such as legislative lag, lack of coordination among law enforcement, and serious disloca-tion of judicial relief. Therefore, under the vital time of performing the strategy of “rule by law”, the development and reform of the legal system for health insurance in China should be promoted. On the basis of summarizing the his-torical development of the legal system for health insurance in China, this paper proposes solutions such as centrali-zing legislative upgrades and expediting the process of unitary and special laws from the perspective of“rule by law”. The system of personal credit should be established and judicial transparency should be promoted to improve the legal system for health insurance.

Cardiovascular disease in peri-menopausal period is a kind of climacteric syndrome which based on the symptoms of the cardiovascular system as the main manifestations. It is manifested as hypertension,pseudo-angina,arrhythmia. Hormone replacement therapy or symptomatic treatment is the common used method of modern medicine,although a certain degree of efficacy,but there are also significant disadvantages. In the treatment of cardiovascular disease in peri-menopausal period,TCM has certain advantages. In this paper,we treat cardiovascular disease in peri-menopausal period from the theory of 'yin deficiency,blood stasis,heat-toxin'and propose the method of 'nourishing yin,promoting blood circulation and detoxification'.

Objective Culture,expansion and characterization of pseudomyxoma peritonei cells to support foundation for the study of pseudomyxoma peritonei and drug screening.Methods Tumors from 5 cases of human pseudomyxoma peritonei were cultured by collagenase digestion.The cultured cells were then identified as tumor cells by chromosome karyotyping.The secretion of neutral mucopolysaccharide was detected by PAS staining.To simulate the tumor growth condition in vivo,3D culture was applied and the morphology of the cultured organoid was observed with HE staining,xenografts were used to test the tumorigenicity of PMP primary cells.Results The primary culture of pseudomyxoma peritonei cells was successful in all of the 5 cases.2D cultured cells were adherent,polygonal pebble-like arranged,and passed on up to 18 generations.Chromosome karyotype analysis showed that most of the cells were subdiploid karyotype tumor cells.Positive PAS staining suggests that these cells secrete mucus.The morphology of 3D cultured organoid was similar to that of tumor tissues.The tumor formation rate of xenografts was low,and the tumoris not similar to patient's PMP.Conclusions Pseudomyxoma peritonei tumor cells can be cultured and expanded in vitro.

Objective:To explore the effect of psychological training on self consistency and congruence of army men. Methods:532 army men were tested by Self Consistency and Congruence Scale(SCCS)、EPQ、simplified coping style questionnaire(SCSQ)and SCL-90. Results:There were significant difference in score of SCCS between army men group and norm.There were significant correlation between factor scores of SCCS and scores of EPQ,SCSQ and SCL-90. After psychological training,the study group had higher score in flexibility than that of norm. The study group had higher score in flexibility and positive coping style than control group,and lower scores in P and N of EPQ,total score,obsessive-compulsive,interpersonal sensitivity,anxiety,hostility,paranoid ideation and psychoticism of SCL-90 than control. Conclusion:Self consistency and congruence level of army men were associated with personality, coping style and mental health. The psychological training has effect on balancing personality,facilitating self consistency,improving coping style and the psychological status of army men.

Objective: To investigate the effect of psychological intervention on mental health status in women who accepted artificial abortion.Methods: 576 cases of women accepted artificial abortion were randomly divided into psychological intervention group and control group,and evaluated their psychological state before artificial abortion and at first visit with SCL-90.Results: Compared with control group,psychological intervention group had obvious improvement in mental health states(P

Objective To compare the efficacy of carbon nanoparticles suspension and methylene blue for the detection of axillary sentinel lymph nodes in rabbits with breast cancer,to provide a method for the clinical application of treatment of breast cancer.Methods The model of breast cancer was established in 40 rabbits by intramammary injection of VX2 tumor tissue suspension 1ml.The successful model was divided into two groups.Carbon nanoparticles suspension and methylene blue were used in staining sentinel lymph node.The staining time,stain fading time of SLN,number of stained SLN,total number of the stained lymph node and the staining effect were observed.Results The staining time of SLN and number of stained SLN had no significant difference between the 2 groups,total number of the stained lymph nodes was 3.25?0.56 and 2.3?0.21 respectively in carbon nanoparticles suspension group and methylene blue group(t=6.99,P

OBJECTIVE:To determine the contents of chloromycetin and dexamethasone acetate in Jiefu cream.METHODS:A reversed-phase HPLC was established for the determination with C18 column as stationary phase.The mobile phase consisted of acetonitrile and 0.05%mol/L phosphate buffer solution(40∶60),the detection wavelength was 240nm.RESULTS:Chlorom-ycetin had a good linearity (r=0.9 998) in the range of 0.1～0.3mg/ml,the mean recovery was 100.6% with a RSD of 1.3%.Dexamethasone acetate had a good linearity(r=0.9 998) in the range of 0.02～0.08mg/ml,the mean recovery was 101.1% with a RSD of 1.7%.CONCLUSION:The method is simple,sensitive and accurate.

Objective To examine whether electroacupuncture at the Wangu acupoint (GB 12 ) , whose position is similar to the cerebellar fastigial nucleus ,can reduce inflammatory cytokines in the hippocampus of rats with vascular dementia (VD) to provide theoretical evidence for treating VD with electroacupuncture .Methods Healthy Sprague‐Dawley rats ( n=54 ,300 -450 g) were randomly divided into three groups :sham surgery group ,VD group ,and electroacupuncture group .The ethologic scores of VD rats were evaluated and the mRNA expressions of inflammatory cytokines (TNF‐α,IL‐6 and IL‐1β) in the hippocampus were assessed and the hippocampal tissues were observed by hematoxylin‐eosin (HE) staining .Results Compared with VD group ,in electroacupuncture group the rats' learning ability improved significantly and the mRNA expressions of TNF‐α, IL‐6 and IL‐1β decreased . Simultaneously ,the damage extent of nerve cells in the hippocampal tissues decreased , and their morphology recovered to nearly normal .Conclusion Electroacupuncture at the Wangu acupoint can decrease the level of inflammatory cytokines in the hippocampus and reduce the damage extent of nerve cells in the hippocampus ,thus providing a new neuroprotective method for VD .