BACKGROUND:Several studies have demonstrated that the physiological process of the ovary, including folicle development, maturity, ovulation, corpus luteum formation and regression, is related to the formation of extracelular matrix. However, matrix metaloproteinase 9 and connective tissue growth factors had a close relationship with the formation of extracelular matrix. OBJECTIVE:To investigate the relationship between the expression of connective tissue growth factor and matrix metaloproteinase 9 and polycystic ovary syndrome. METHODS:The female SD rats with regular estrous cycle were randomly divided into control and model groups. The rats in the model group were intragastricaly with letrozole to establish the model of polycystic ovary syndrome, while the rats in the control group were intragastricaly with the same amount of carboxymethyl celulose. When the natural law of estrous cycle of rats in the model group did not exist and consecutive interval appeared, rat serum and ovarian tissues were colected. Rats in the control group were detected by colecting specimen in the interval. RESULTS AND CONCLUSION:Radioimmunoassay and immunohistochemical staining results showed that compared with the control group, the expression of serum luteinizing hormone, serum prolactin levels, connective tissue growth factor in preantral folicles theca cytoplasm, connective tissue growth factors in folicular basement membrane cytoplasm, connective tissue growth factors in albuginea and matrix metaloproteinase 9 in corpus luteum significantly increased (P < 0.05). The contents of folicle stimulating hormone and serum estradiol, and expression of matrix metaloproteinase 9 in folicular basement membrane significantly decreased (P < 0.05). The results indicate that connective tissue growth factors may be related to the excessive growth of smal folicles and ovulation disorders in polycystic ovary syndrome, while matrix metaloproteinase 9 may be related to ovulation disorders in polycystic ovary syndrome.

Objective:To study the effect of insulin like growth factor 1 and insulin on the promoter activity of human ?1(Ⅰ) procollagen (COL1A1) gene and the influence of tumor necrosis factor ?(TNF?), interferon ? (IFN?) and interferon ?(IFN?) on the effect Methods:Two deletion constructs,pCOLH0 27 and pCOLH2 5 ,containing portions of 5' flanking region of the gene ( 268～+42 bp and 2 483～+42 bp respectively) ligated to the chloramphenicol acetyltransferase (CAT) reporter gene ,were transiently transfected into human skin fibroblasts The cells were subsequently treated with IGF 1 or insulin, and TNF? or IFN? or IFN? The CAT activity was assessed 24 h after the cytokines added Results:The results showed that 13 nmol/L IGF 1 caused 3 68 fold and 4 04 fold increase in the CAT activity of pCOLH0 27 and pCOLH2 5 respectively and that 2 5 ?mol/L insulin caused 3 69 fold and 3 93 fold increase in the activity of the two plasmids respectively TNF?,IFN? and IFN? reduced IGF 1 or insulin induced promoter activity of pCOLH2 5 Conclusion:IGF 1 and insulin could increase the promoter activity of the human (COL1A1) gene TNF?,IFN? and IFN? could suppress the promoter activity induced by IGF 1 and insulin

Hyperphenylalaninemia is a common group of inherited metabolic diseases.It is characterized by the increased concentration of plasma phenylalanine.The metabolism of phenylalanine requires phenylalanine hydroxylase and coenzyme tetrahydrobiopterin.These enzymes cannot function normally if there is any mutation in their encoding genes.Children suffering from hyperphenylalaninemia without promptly treatment may present mental development delay and other serious nervous system sequelae.With the technical improvement of molecular genetics,there have been many progresses in the study of genetic diagnosis,genotype-phenotype correlation and gene therapy of hyperphenylalaninemia.This article reviews the history and classification of hyperphenylalaninemia,the characteristics of gene mutation,the methods of genetic diagnosis,the genotype-phenotype correlation,and the progress of new therapy.

Objective:To explore application value and therapeutic effect of self‐efficacy scale in postoperative nurs‐ing of patients undergoing coronary artery bypass grafting (CABG) .Methods :A total of 92 patients after CABG treated in our hospital from Mar 2013 to Mar 2014 were selected .According to random number table ,patients were randomly and equally divided into routine nursing group (received routine nursing) and self‐efficacy group (received self‐efficacy management ) .Scores of the medical outcomes study 36‐item short‐form health survey (SF‐36) and gen‐eral self‐efficacy scale (GSES) were compared between two groups in 24h after hospitalization ,at discharge ,and one ,three ,six months after discharge .Results :In 24h after hospitalization ,there were no significant difference in scores of SF‐36 and GSES between two groups ,P>0.05 both .Compared with routine nursing group at discharge , and one ,three ,six months after discharge ,there were significant rise in scores of GSES [one month:(2.50 ± 0.49) scores vs .(2.90 ± 0.41) scores ,six months:(2.72 ± 0.47) scores vs .(3.32 ± 0.33) scores] and SF‐36 [one month :(73.22 ± 6.88) scores vs .(79.45 ± 7.32) scores ,six months:(83.62 ± 4.77) scores vs .(93.46 ± 3.65) scores] in self‐efficacy group ,P<0.01 all .Conclusion:Self‐efficacy management can facilitate improvement of patient's confi‐dence ,their behavior changes or adjustment ,and their efforts on rehabilitation training after CABG .It shows good prospects and social benefits ,which is worth extending .

Sonic Hedgehog (SHH) signaling pathway not only plays key roles in embryonic development,but also functions in postnatal development and maintenance of tissue/organ integrity and function.In recent years,it has been found that the SHH signaling pathway was abnormally activated in some lung diseases,which suggested that the SHH signaling pathway may play a part in the development of some lung diseases.This article reviewed the potential role of SHH signaling pathway on the embryonic lung development and lung diseases.

In recent years,food intolerance has gradually become the focus of public attention.Food intolerance is a complicated allergic disease,which is different from food allergy.It may lead to systemic diseases.Because of its hidden and no specific symptoms,the diagnosis is often difficult.Through the detection of food specific IgG antibody,no intake or less intolerance food intake,can effectively improve the patient's symptoms,control and prevention of disease.This article reviews food intolerance from six aspects including its pathogenesis,the difference of food allergy,morbidity,the relationship with pediatric headache,the evaluation of diagnostic technique and nutrient adjustment.

Objective:To study the effect of synthetic antimicrobial peptides on Escherichia coli biofilm.Methods:The test tube method was used to establish the model of Escherichia coli biofilm.The samples were randomly divided into four groups:group A,by adding 4?MIC antimicrobial peptides to the samples,group B,by adding 1?MIC gentamicin to the samples,group C,by adding 4?MIC antimicrobial peptides and 1?MIC gentamicin to the samples,and group D,by adding phosphate buffered saline to the samples as a control.After 6 h the viable bacteria in the biofilm were counted following the method of colony count.The different changes in the biofilm were compared between the treated groups and the control group with scanning electron microscope.Results:The viable bacteria in group C were significantly decreased compared with the other three groups(P≤0.05).While compared with group D,the viable bacteria were decreased in group A and group B(P≤0.05).From the graph of scanning electron microscope the integrity of the Escherichia coli cell membrane was disrupted in group A and C.Conclusion:The results suggest that the killing effect of combination of antimicrobial peptides with gentamicin is significantly increased.

316 patients in the department of obstetrics and gynecology were treated with microwave radiotherapy. intrauterine microwave radiotherapy was used in 110 patients with uterine hemorrhage (35 dysfunctional bleeding. 54 hysteromyoma, 21 other menorrhagia). The date showed that the effective rate was 96.4% In 206 patients with inflammatory diseases and other perineal diseases (107 inflammation in incisional wound, 16 breast and pelvic inflammatory mass, 23 induration in incisional wound, 34 perineal edema, and 6 perineovaginal hematoma), the effective rate was 98.5%. No side effect was found in these patients. It is considered that the microwave radiotherapy is convenient, ecnomical, painlees, and worth to be recommended in the field of obstetrics and gynecology. The mechanism of microwave action on the tissue and evaluation of microwave radiotherapy are discussed

Objective To screen empowerment psychological care scale items for critically ill patients through Delphi method.Methods Totally 27 experts were invited to conduct two rounds consultation by using Delphi method,and the results were summarized.Results The effective response rate of the experts' questionnaire for the first round and the second round was 90％ and 100％.The specialist authority coefficient was 0.895.The coordination coefficients for 2 rounds were 0.432 and 0.448 respectively.After two-round consultation,empowerment psychological care scale items for critically ill patients was finally formulated,containing 4 dimensions and 24 evaluation items.Conclusions The selected experts were highly representative,and they were enthusiastically involved in the consultation.And the scale has remarkable authority and coordination;it can reflect the theme of the study,provide instructive function for clinical medical personnel in implementation and evaluation of empowerment psychological care.

Objective To establish the method for detecting human Bocavirus (HBoV) by a real-time fluorescent quantitative PCR (FQ-PCR) and to assay HBoV from nasopharyngeal samples of children with acute respiratory tract infections.Methods The specific primers and Taqman probes of targeted HBoV NP1 gene were designed.The aimed fragment of NP1 gene was amplified with PCR and ligated into a pCEM-T Easy vector to set up the standards.The method for detection of NP1 gene using FQ-PCR was established.The sensitivity and specificity of the method had been tested.Then 195 clinical specimens were subsequently tested.Results The specificity of this method was excellent.The linear amplification of the assay ranges from 10 copies/μl to 10~8 copies/μl.Twelve specimens were tested positive for HBoV in 195 clinical specimens (6.2%).Conclusions The method for detection of HBoV NP1 geneby real-time fluorescent quantitative PCR was established successfully.It can offer fast and reliable diagnosis for HBoV infection.