Objective To investigate the clinical significance of the detection of the levels of serum uric acid (UA) and serum eytokine tunor necrosis factor-oα (TNF-α),soluble intercellular adhesion molecule-1 (sICAM-1),interleukin (IL)-6 and IL-8 in patients with chronic obstructive pulmonary disease (COPD).Methods UA of 40 healthy people (control group) and 158 COPD patients (COPD group) in acute exacerbation and remission stage was detected respectively by automatic biochemical analyzer,and the levels of serum TNF-α,sICAM-1,IL-6 and IL-8 were detected by ELISA.The percentage of first second forced expiratory volume in expected value (FEV1％) of COPD patients in acute exacerbation and remission stage were detected.Results TNF-α,sICAM-1,IL-6,IL-8 and UA of COPD group in acute exacerbation were significantly higher than those of control group (P＜ 0.01 ),and FEV1％ was significantly lower than that of control group (P＜ 0.01 ).TNF-α,sICAM-1 and UA of COPD group in remission stage were significantly higher than those of control group (P ＜0.01 or ＜0.05),IL-6 and IL-8 had no statistical significance compared with those of control group (P ＞ 0.05),and FEV1％ was significantly lower than that of control group (P ＜ 0.01 ).Correlation analysis showed that TNF- α,sICAM-1,IL-6,IL-8,UA of COPD patients in acute exacerbation were negatively conrelated with FEV1％ (r =-0.681,-0.579,-0.545,-0.652,-0.357,respectively,P ＜ 0.01 or ＜ 0.05 ).In remission stage,TNF- α,sICAM-1,IL-6 and IL-8 of COPD patients were negatively correlated with FEV1％ (r =-0.623,-0.335,-0.349,-0.623,P＜0.01 or ＜0.05),and UA had no significant correlation with FEV1％ (r =-0.092,P ＞0.05).Conclusions The serum UA and cytokines TNF-α,sICAM-1,IL-6,IL-8 are involved in the progression of COPD and correlated with lung function.They can be used as the judge indexes of COPD.

ObjectiveTo explore the clinical application of pleural mesothelial cell (PMC) determination in the diagnosis of pleural effusion.MethodsPMC of 36 patients with malignant pleural effusion (malignant group) and 24 patients with benign pleural effusion (benign group) were counted to determine the clinical value of PMC in benign and malignant pleural effusion.ResultsIn malignant group,there was no case with PMC disappear,1 case(2.78％) with the percentage of PMC between 0 and 1％,1case(2.78％) between 1％ and 5％,34 cases (94.44％) more than 5％.In benign group,there were 11 cases (45.83％) with PMC disappear,8 cases (33.33％) with the percentage of PMC between 0 and 1％,2 cases (8.33％) between 1％ and 5％,3 cases( 12.50％ ) more than 5％.The percentage of PMC between two groups had significant difference (x2 =43.4069,P ＜ 0.01).95％ confidence intervals of PMC in benign and malignant group respectively were 0.28％ -5.10％ and 5.13％ -10.91％,which did not overlap.The percentage of PMC more than 5％ was defined as the diagnosis standard of identifying benign and malignant pleural effusion,the sensitivity of the diagnosis was 94.44％(34/36),the specificity was 87.50％(21/24) and the accuracy was 91.67％(55/60).ConclusionsPMC determination is a kind of easy,economical and fast way to diagnose benignand malignant pleural effusion with high sensitivity,specificity and accuracy.Therefore,it has reference value in clinical diagnosis.

Since a number of genes have been discovered,which are associated with the effect of drug therapy,the development of personalized medicine is enhanced.Meanwhile,personalized test played an important role in choosing proper medicine.Herein,the status of detection of V-ki-ras2 Kirsten rat sarcoma viral oncogene homolog(Kras),epidermal growth factor receptor(EGFR),B-Raf and v-Raf murine sarcoma viral oncogene homolog B1(BRAF) mutation is concluded,moreover,the association between the mutation test and the effect of cancer therapy is summarized.

Objective To evaluate the values of combined detection of C-reactive protein ,white blood cell count and neutrophil ratio in infection .Methods 158 patients diagnosed with infection were divided into bacterial infection group and non-bacterial infec-tion group .The concentrations of C-reactive protein ,white blood cell count and neutrophil ratio were detected ,which were compared with cases in the control group .Results The concentrations and abnormal rates of C-reactive protein ,white blood cell count ,neu-trophil ratio in bacterial infection group were significantly higher than those in non-bacterial infection group and control group(P<0 .01) .The concentration and abnormal rate of C-reactive in non-bacterial infection group were significantly higher than those in control group(P<0 .05) .Conclusion Determination of C-reactive protein can supplement the lack of white blood cell count and neutrophil ratio ,determine the degree of inflammation or injury .The combined detection of C-reactive protein ,white blood cell count and neutrophil ratio in infection has important clinical significance .

BACKGROUND:Many researches on the development of cochlea have been made, but mainly depend on the pathological conditions and developmental deformity, while the researches on the development process of normal embryonic cochlea and expression of related factors are rare.
OBJECTIVE:To investigate the expression of transforming growth factorβ1 in rat embryonic cochlea.
METHODS:Fifty-six Sprague Dawley rats were selected and the rats were pregnancy, and the embryos were obtained from the rats, trimmed the inner ear specimens under the anatomical microscope, and then the specimens were treated with dehydration, decalcification, directly paraffin embedding and slicing processing. The morphology evolution of inner cochlea was observed under light microscope after hematoxylin-eosin staining. The expression of transforming growth factorβ1 in rat embryonic cochlea was observed by immunohistochemical streptavidin biotin-peroxidase complex method.
RESULTS AND CONCLUSION:The development of rat inner ear began in ectoderm thickening area of E8 phase, otocyst and the emergency of the mesenchymal could be seen in E9 phase, otocyst and ossicular chain began to develop in E9.5 phase, formation of cochlear duct anlage could be seen in E12.5 phase, cochlea tube development was completed in E18.5 phase and Corti’s formation could be seen in E16 phase. The structure and function of the inner ear could be ful y developed after birth. The expression of transforming growth factorβ1 could be seen in E12.5-E19 phases, and the expression was changed from weak to strong and then weakened further, and strongest in E14.5 phase. This suggested that transforming growth factorβ1 may be involved during the development of rat cochlear epithelium.

Objective To explore the effect of simvastatin and atorvastatin on the level of p 27 protein in mesangial cells of rat under high glucose .Methods Cultured rat mesangial cells in vitro were divided into four groups :normal glucose(5 .6 mmol/L glu-cose) ,high glucose(30 mmol/L glucose) ,high glucose(30 mmol/L glucose) plus simvastatin(10 μmol/L) ,high glucose(30mmol/L glucose) plus atorvastatin(10 μmol/L) .After treatment for 24 ,48 ,72 120 hours ,the cells were collected .The protein level of p27 was detected by Western blotting and the mRNA level of p27 was detected by RT-PCR .Results Compared to the normal glucose group ,the levels of p27 protein and mRNA in the high glucose group had a significant increase(P<0 .05) .Compared to the high glucose group ,the level of p27 protein and mRNA in the high glucose plus simvastatin or atorvastatin group both had a significant decrease(P<0 .05) .Conclusion By modulating the expression of P27 in mesangial cells ,statins could become the independent lipid-decreasing drug to prevent kidney .

Objective To discuss oral cavity nursing in senile diabetic patients with oral disease.Methods 80 senile diabetic patients were offered diet control and treatment for lowering blood glucose with simultaneous oral nursing for 4 weeks.The blood glucose,blood lipid before and after nursing were observed.Results The total effective rate reached 91.3% after oral nursing,blood glucose and blood lipid greatly lowered after nursing.Conclusions Prevention of oral disease is important for preventing diabetic complications,appropriate oral nursing contributes to prevention and treatment of diabetes mellitus complicated with oral disease.

Based on the randon investigation of medical students in the library through the questionnaire designed independently,the paper takes the library of Jining Medical University as an example to understand the situation of the use of library website by medical students in new media environment,and points out that the library should understand the information requirements of medical students in new media environment,be closely related to course teaching,give play to subjective initiative of the librarian,and make full use of the new media platform.

There exists different view in the goal of investigative study.Based on the self-fulfilling cycles of self-regulated learning,the author of the article puts forward that developing the ability of self-regulated learning by doing investigative study according to each phase characteristic of self-regulated learning is one of the important goals of investigative study.

Objective To study the relationship between the number of myeloid-derived suppressor cells (MDSCs) in peripheral blood and prognosis in patients with peripheral T-cell lymphoma (PTCL). Methods Twenty-three patients with PTCL were selected in this study and 14 healthy persons were used as the normal control. The proportion of MDSCs/mononu?clear in peripheral blood was detected by flow cytometry. The correlation between the number of MDSCs and the clinical character and prognosis in patients with PTCL was analyzed. Results The proportion of MDSCs in peripheral blood was sig?nificantly higher in patients with PTCL than that of healthy subjects [(4.59±1.41)%vs (1.36±0.59)%, P<0.01]. The number of MDSCs in peripheral blood was significantly decreased after chemotherapy compared with that before treatment [( 1.29 ± 2.95)%, P<0.05)]. There were significant differences in MDSCs expressions in patients between different extranodal involve?ment, bone marrow involvement, clinical stage and IPI score (P<0.05). Conclusion The higher expression of MDSCs may be related to the progression in patients with PTCL.