BACKGROUND: Being a kind of anti-oxidizing agent, vitamin E has the function of eliminating free radical and reducing the free radical injury of antioxidant enzyme in sports, which can alleviate appearance of fatigue and improve sports ability. OBJECTIVE: Though the intervention of vitamin E upon exercise-induced muscle damage (EIMD), the study was designed to investigate the effect of vitamin E on the morphology of EIMD and to provide the experimental basis of research on morphology and mechanism of EIMD. DESIGN, TIME AND SETTING: A randomized block animal experiment was performed in the Key Laboratory of General Administration of Sport of China in Shenyang Sport University and the Microelectronic Laboratory of Chinese Medical University from May to October 2007. MATERIALS: A total of sixteen male Sprague Dawley rats were randomly divided into four groups: control, exercise, normal saline, and vitamin E groups. METHODS: The rats in the vitamin E group was administered (i.p.) 1-1.2 mg/kg vitamin E capsules once every eight hours four times one day before experiment, and the amount of injecting volume was 4 mL/kg. The rats in the normal saline group were administered (i.p.) normal saline with same volume and time. The rats in the exercise group only took some exercises without administering vitamin E or normal saline. The rats in the control group received not any intervention and treatment. The injury model was established by taking a bout of exhaustive downhill running. The triceps brachii muscles extracted from the right side of rats after exercise were prepared for electron microscope specimens. MAIN OUTCOME MEASURES: The arrangement of myofibrils and sarcomeres, the changes of Z-line, and the morphology changes of cell membranes, nucleus, mitochondria, sarcoplasmic reticulum, transverse tubule, and satellite cells were observed. RESULTS: In the vitamin E group, the muscular ultrastructures of EIMD were improved obviously. Though the sarcoplasmic reticulum showed edema, the regular arrangement of myofibrils was observed. The light and dark zone of Z-line and nucleus appeared clearly. CONCLUSION: Supplement of vitamin E could decrease the muscular morphological changes of EIMD after eccentric exercise. The arrangement of myofibrils was more regular and sarcomeres appeared more clearly. The function might relate to the antioxidation and promoting synthesis of proteins of vitamin E.

OBJECTIVE:To observe the hypotensive effect of Urapidil in operation on patients with fundus oculi disease accompanying hypertension.METHODS:Urapidil was injected iv first and then dripped.The SP,DP and heart rate were ob?served at given times and ECG was monitored.RESULTS:The total effective rate was95.1%,average SP decreased43.97mmHg(P

Objective To investigate on the students' network usage,addiction and influencing factors at a certain university and analyze its correlation with impulsive choice.Methods Multi-class sampling method was applied.1 777 students were selected from the School of Clinical Medicine,School of Nursing,School of Public Health,School of Mental Health,School of Medical Laboratory Science,Department of Stomatology,School of Pharmacy,School of Management and Department of Biology,including 1 534 medical majors,accounting for 86.3％ and 243 non-medical majors,accounting for 13.7％.Results 121 students were addicted to the internet,accounting for 6.8％ of the selected students,while 1 656 students were free from the internet addiction,accounting for 93.2％ of the selected students.Among all the addicts,96 were from medical majors,accounting for 79.3％ and 25 were from non-medical majors,accounting for 20.7％.As for the online activities,boys loved computer games while girls preferred to watch movies,listen to music and shop.At the same time,most of the junior students and non-medical majors played games online.As for the length of time,students spending 5 and more hours everyday on the internet accounted for 3.4％.As for the sites,medical majors usually used their cell-phones while non-medical majors mostly got on line at their dorms.Probably due to the boys' preference for computer games,a larger proportion of boys getting on line at school dorms and internet bars off the campus.Most of the girls chose to use their cell-phones.More junior students surferred the internet at dorms while more senior students used their cell-phones.As for the function of the medical knowledge on the prevention and treatment of internet addiction,9.8％ of the students held that medical knowledge had a great effect on the prevention and treatment of internet addiction,61.5％ believed in a general effect,while 28.6％ of the students thought it useless.The multi-factor analysis showed that grade,home address and the communication with parents all attribute to internet addiction.Internet addiction was significantly associated with impulsiveness,and 3 dimensionality of impulsive choice (P＜ 0.01).Conclusion There is higher incidence of internet addiction among the junior students,students from the urban areas and those who lack communication with parents.Grade,home address and situation of communicating with parents are the important factors for internet addiction.Internet addiction is close association with impulsive choice.

Objective To explore the relationship between osteoporosis knowledge and activities and fear of falling of elderly in the community.Method 420 elders in community were investigated with osteoporosis knowledge assessment tool (OKAT) and the modified survey of activities and fear of falling in the elderly (MSAFFE).Result The overall average score of MSAFFE was (25.36±4.58),and exercise capacity dimension scored higher (13.89±2.41).The overall average score of OKAT was (9.11±3.81).The correlation coefficient of MSAFFE and OKAT was-0.11～ 0.58(P＜0.05).The level of knowledge osteoporosis could explain 24％,18％,16％ variation of activities and fear of falling in each dimension.Conclusion The level of knowledge osteoporosis in the elderly is closely related to activity and fear of falling.Health education should be strengthened to improve the life quality of older people.

Objective To investigate the origin of prostatic blood-supply by digital subtraction angiography (DSA) and provide references for the arterial embolization of prostatic hyperplasia. Methods A total of 72 male patients with successful selective and super-selective prostatic arterial catheterization angiographies were observed and recorded the original arterial blood-supply of the prostate, number of the prostatic arteries and the predominant blood supply. Results The blood-supply of prostate is very complicated including the original prostatic arterial supply from the inferior vesicle arteries (69); the internal iliac arteries (63); the internal pudendal arteries (52); the inferior rectum arteries (29); the superior vesicle arteries (14). The predominant arterial supply include the internal iliac arteries (37); the inferior vesicle arteries (20); the internal pudendal arteries (6) and the inferior rectum arteries (2). Conclusion Arterial contrast medium angiography can correctly demonstrate and assess the original arterial supply of prostate, providing significant guidance for further interventional embolization.

Objective: To study the clinical manifestations of the head and neck angiosarcoma and the re-lationship of the staging with clinical treatment and prognosis. Methods: The clinical data of 21 patients with head and neck angiosarcoma treated in our hospital between January 1993 and January 2008 were retro-spectively analyzed. By studying the medical records of all patients and reviewing the related literatures, we discussed the clinical manifestations of these patients and the relationship of staging with clinical treatment and prognosis of head and neck angiosarcoma based on soft tissue sarcoma staging of AJCC2002' 6th. SPSS 17.0 software was used for statistical analysis. We calculated the survival rate using Kaplan-Meier method and compared the survival curves using log rank test. Results: Among these 21 cases, 10 were treat-ed with non-combined treatment, and the other 11 cases were treated with combined treatment. There were 6 stage Ⅰ cases, 3 stage Ⅱ cases, 1 stage Ⅲ case, and 11 stage Ⅳ cases. The 1-, 3-, 5- and 10-year total sur-vival rates were 72.2%, 41.3%, 27.5% and 13.8%, respectively. The average time of recurrence or metastasis was 4 months. Twelve cases had local recurrence (accounting for 57.1%), and 11 cases had distant metasta-sis (accounting for 52.4%). The metastatic sites included lymph node in 5 cases, lung in 4 cases, liver in 1 case, and bone in 1 case. Different treatment modalities (χ~2=8.31, P=0.004) and tumor staging (χ~2=9.74, P=0.002) were prognostic factors. Conclusion: There are no unified treatment strategies for angiosarcoma of the head and the neck. The overall prognosis is still not ideal. Biological treatment has been applied to clinical practice. Preoperative combined treatment has positive results. Tumor staging is an important factor affecting the prognosis. Surgery-based combined treatment can improve patient survival.

Cell Line, Tumor ; Humans ; Leukemia-Lymphoma, Adult T-Cell ; metabolism ; NF-kappa B ; metabolism ; Proto-Oncogene Proteins ; metabolism ; Transcription Factors ; metabolism

BACKGROUND:Studies have found that β-casomorphin 7 can lessen oxidation of renal tubular epithelial cels and lower blood sugar in a rat model of diabetes. There is a few studies concerning the effect of β-casomorphin-7 on diabetic nephropathy. OBJECTIVE:To explore the effects of β-casomorphin-7 on renal injury in a rat model of diabetic nephropathy. METHODS:The 30 rats were equaly and randomly divided into control group, model group andβ-casomorphin-7 group. Twenty rat models of diabetes were established. In the model and β-casomorphin-7 groups, rats were intraperitonealy injected with streptozotocin (60 mg/kg). In the control group, rats were intraperitonealy injectedwith an equal volume of citric acid. Rats in the β-casomorphin-7 group were intragastricaly administeredβ-casomorphin-7 for 30 days. Rats in the model and control groups were given an equal volume of saline by intragastric administration. RESULTS AND CONCLUSION: (1) Compared with the control group, insulin levels decreased, glucagon, blood glucose, urine glucose, urine protein, serum creatinine, serum urea nitrogen levels, mRNA and protein expression levels of type I colagen and type IV colagen in kidney increased in the model group. (2) Compared with the model group, above indexes were apparently recovered in the β-casomorphin-7 group. These findings indicate that β-casomorphin-7 has a protective effect on renal injury in diabetic nephropathy rats.

BACKGROUND: Safflower yel ow is known to treat diabetic nephropathy, can protect kidney function, reduce lesions, delay or prevent the development of diabetic nephropathy.
OBJECTIVE: To further verify the effects of safflower yel ow on the kidney in rats with diabetic nephropathy and the impact of safflower yel ow on the expression of renal cel receptor of angiotensin II1.
METHODS: Thirty rats were equally and randomly divided into three groups: normal control group, model group and experimental group. In the experimental group and model group, rat models of diabetic nephropathy were established. At 1 week after model establishment, rats were daily intraperitoneally given safflower yellow injection 27.8 mg/kg, once a day. In the model and control groups, rats were daily intraperitoneally given an equal volume of saline. All rats were administered for 23 weeks. The kidney-related biochemical indicators and pathological changes in kidney tissue and the expression of angiotensin II1 type receptor on kidney tissue of rats were observed.
RESULTS AND CONCLUSION: (1) Hypertrophy index of experimental group and model group was significantly higher than that of control group (P < 0.05). (2) 24-hour proteinuria, glycosylated hemoglobin, total cholesterol, triglycerides, creatinine and urea nitrogen of model group were higher than that of control group (P < 0.05). 24-hour proteinuria, glycated hemoglobin, total cholesterol and urea nitrogen in experimental group were higher than that of control group (P < 0.05). 24-hour proteinuria, total cholesterol, triglycerides, creatinine and urea nitrogen in the experimental group were lower than that of model group (P < 0.05). (3) Glomerular hypertrophy, thickening of capillary basement membrane, cell proliferation, and mesangial widening were detected in the model group. Abnormal changes in the kidney structure were found in the control group. The extent of damage of kidney of histopathology in the experimental group was between the control group and the model group. (4) The expression level of angiotensin receptor II1 of experimental group and model group was higher than that of control group (P < 0.05). The expression level of angiotensin receptor II1 of experimental group was lower than that of model group (P < 0.05). These results indicated that safflower yellow has a protective effect on diabetic nephropathy; the mechanism of action may be associated with blocking kidney partial renin-angiotensin system.

Objective To establish a method for detecting intestinal bacteria in Caenorhabditis elegans.Methods The gut flora number of 1, 20 or, 50 C.elegans was quantified and compared using real-time quantitative PCR (qPCR). Results The gut flora of a single C.elegans could be detected using qPCR method , which could also reflect the difference in the number of gut bacteria between different samples .Conclusion The qPCR method can be uased to accurately quantify intestinal bacteria even in only one C.elegans and has the advantages of low-cost, high-sensitivity and good-specificity.