Objective To summarize the experience of nursing cooperation in endoscopic sinus surgery for low temperature plasma radiofrequency ablation of skull base tumor, and to explore the safety and feasibility of this technique. Methods A total of 69 patients with skull base tumor were treated with low temperature plasma radio frequency technology to remove skull base tumor. Results Totally 62 cases of skull base tumors completed total resection in low temperature plasma radiofrequency ablation, 7 cases gained subtotal resection. The operation demonstrated superior advantage of less bleeding, short operation time, less postoperative pain and no adverse events. Conclusions The skull base tumor resection for patients with skull base tumors with low-temperature plasma radiofrequency possesses the advantages of short operation time, less bleeding and thermal damage to the surrounding tissues, besides it can reduce the postoperative discomfort, shorten hospitalization time and reduce the occurrence of nursing adverse events.

In recent years,the influencing factors of prognosis and effect after radiotherapy in breast cancer patients with radical mastectomy or modified radical mastectomy have gradually become a hot research,and it is closely related with the stages,subtypes,hormone levels and the body mass index (BMI).Otherwise,from three dimensional conformal radiation therapy to helical tomotherapy,the development of radiation technology has a breakthrough progress and makes the treatment of diseases more accurate and rational.

Acupuncture Points ; Acupuncture Therapy ; Humans ; Male ; Middle Aged ; Raynaud Disease ; therapy

Combined Modality Therapy ; Congresses as Topic ; Head and Neck Neoplasms ; therapy ; Humans

Acupuncture Therapy ; methods ; Adult ; Ankle ; Female ; Humans ; Male ; Middle Aged ; Stress Disorders, Traumatic, Acute ; therapy ; Wrist

Objective To investigate the effect and the underlying mechanism of miRNA (miR)-506 regulating transforming growth factor-β1 (TGF-β1)-induced endothelial-mesenchymal transition of human aortic endothelial cell lysates.Methods In this study,miR-506 and inhibitory members of the ASPP family (iASPP) were selected as the study objects.First,the expression of miR-506 in human aortic endothelial cell lysates (HAEC) lysate was detected under different concentrations of TGF-β1.The expression of platelet endothelial cell adhesion molecule-1 (CD31),endothelium-cadherin (VE-cadherin),fibroblastspecific protein-1 (FSP1),α-smooth muscle actin (α-SMA) and N-cadherin was determined after miR-506 mimics/mimic negative control (NC) transfection into HAEC treated with or without TGF-β1.Then the expression levels of iASPP in miRNA-506 mimics/mimic NC-transfected HAECs treated with or without TGF-β1 were determined.Finally,the protein expression of CD31,VE-cadherin,FSP1 and α-SMA in HAEC transfected with miR-506 mimics/mimic NC was determined after iASPP overexpression.Results TGF-β1 can induce human aortic endothelial cell mesenchymal transition,down-regulate CD31 and VE-cadherin expression while up-regulate FSP1,α-SMA and N-cadherin expression;TGF-β1 inhibited miR-506 expression,promoted iASPP expression;miR-506 transfection into HAECs up-regulated CD31 and VE-cadherin expression while up-regulate FSP1,α-SMA and N-cadherin expression;miR-506 could downregulate the expression of iASPP;the overexpression of iASPP increased the expression of FSP1,α-SMA and N-cadherin,down-regulated CD31 and VE-cadherin and promoted the transformation of human aortic endothelial cells.Conclusions The transfection of iASPP plasmid promoted the endothelial-mesenchymal transition of human aortic endothelial cell lysates.The transfection of miR-506 inhibited the expression of iASPP and inhibited the endothelial-mesenchymal transition of human aortic endothelial cell lysates induced by TGF-β1.

Animals ; Male ; Mice ; Mice, Inbred BALB C ; Myocarditis ; metabolism ; virology ; Myocardium ; metabolism ; Osteopontin ; metabolism ; Virus Diseases ; metabolism

Biosplint splinting ribbons and flowable restorative were used to stabilize the mobile anterior teeth and replace the missed teeth with in 30 patients severe periodontitis.1 and 2 years after treatment the effective ratio was 86.7% and 83.3% respectively(P >0.05), probing depth and attchment loss were decreased(P <0.05).

Objective To investigate the prognostic value of CD44V6, MMP-9, p57kip2 and hCG in diagnosis differential diagnosis and malignant transformation of hydatidiform mole. Methods The expressions of CD44V6 MMP-9 and p57kip2 was detected by SP method and the serum hCG was detected with CLIA method in 55 cases of hydatidiform mole, 20 cases of abortion villi and 10 cases of normal villi, and the value of CD44V6, MMP-9, p57kip2 and hCG in diagnosis differential diagnosis and malignant transformation of hydatidiform mole was analyzed. Results There were 9 cases malignant transforming hydatidiform mole and 44 cases of non-malignant transforming hydatidiform mole(20 cases of complete hydatidiform mole, 26 cases of partial hydatidiform mole). The expression of CD44V6 MMP-9 and p57kip2 of malignant transforming mole was significantly higher than that of non-malignant transforming group (77.8 % vs 30.4 %, 77.8 % vs 34.8 %, 11.1 % vs 58.7 %) (P <0.05) and the expression difference of p57kip2 in complete mole and partial mole group (5.0 % vs 100.0 %) was statistically significant (P <0.05). HCG remained positive in 4 cases of hydatidiform mole and dropping and then rised in 5 cases. The sensitivity of p57kip2 in the diagnosis of partial hydatidiform mole was 100.0 %, the specificity was 95.0 %, the negative prediction was 100.0 %. The sensitivity of CD44V6 in the diagnosis of malignant hydatidiform mole was 77.8 %, specificity was 69.6 %, negative prediction was 94.1 %;The sensitivity of MMP-9 was 77.8 %, specificity was 65.2 %, negative prediction was 93.8 %. The sensitivity of the two combined detection (CD44V6 and MMP-9) was 88.9 %, negative prediction was 96.3 %. The sensitivity of combined detection (CD44V6, MMP-9 and hCG) was higher than any of them. Conclusion p57kip2 is an important marker in differential diagnosis of hydatidiform mole. CD44V6, MMP-9 play important roles in the process of transformation of hydatidiform mole. The combined detection of CD44V6, MMP-9, p57kip2 and hCG can help to diagnosis and differential diagnosis and predict biological behaviors and prognosis of hydatidiform mole.

Objective To evaluate the efficacy and safety of pramipexole in treating restless legs syndrome (RLS).Methods A search for randomized,double-blind,and placebo-controlled clinical trials of pramipexole in treating moderate to severe RLS using CNKI,PubMed,Embase and Cochrane Library database was carried out. A meta-analysis of included clinical trials was performed with RevMan 5.0 software.The 2 outcomes that the weighted mean difference(WMD) of change from baseline in International RLS Study Group rating scale(IRLS) score and the relative risk (RR) of response based on the Clinical Global Impression-Improvement (CGI-I) scale score were calculated for efficacy.Safety was assessed with RR of the adverse event (AE).Results A total of 5 clinical trials were included in this meta-analysis,of which 1776 patients were randomly assigned (945 on pramipexole,831 on placebo).The records of patients were pooled.Overall WMD were - 6.34 ( Z =12.76,P ＜ 0.01 ) for the change from baseline in IRLS score,and RR of response based on CGI-I were 1.65 (Z =10.39,P ＜0.01).The overall RR of pramipexole versus placebo were 1.14 ( Z =1.87,P =0.06 ) for AE.Conclusion To treat RLS,pramipexole is an effective and safe drug.