Objective To evaluate prognostic effects and complications of interventional embolization at different time points on patients with ruptured intracranial aneurysms of Hunt -Hess Ⅳ-Ⅴlevels.Methods Through a retrospective analysis from 2008.1 to 2012.12, we collected 162 from 200 patients who underwent interventional embolization due to ruptured intracranial aneurysms according to the criteria.Patients with serious neurological disorders , coma, and end-stage diseases were excluded.Brain CT scan was performed to identify the spontaneous subarachnoid hemorrhage of the patients , followed by CTA or digital subtraction angiography ( DSA) to ensure the accurate diagnosis of intracranial aneurysms.All of the patients at Hunt-HessⅣ-Ⅴlevels were treated with interventional embolization , and were divided into two study groups:early stage therapy group of 79 patients ( embolization at the time <72 hours after the onset) and late stage therapy group of 83 patients ( embolization at the time ≥72 hours after the onset ).Factors including pre-and post-interventional Glasgow coma score ( GCS ) and Glasgow outcome score , ( GOS) as well as barthel index ( BI ) were statistically compared between the study groups.T-test was performed to compare the data between the two study groups.Chi-square test was taken to evaluate the enumeration data.And the correlation of therapeutic time and prognosis of patients was demonstrated by binary regression analysis.Results In the patients with ruptured intracranial aneurysms at Hunt-Hess Ⅳ-Ⅴlevels, early stage intervention (63.5 ±13.5) resulted in significantly better BI after three months , and demonstrated better recovery in comparison to the late stage intervention group (34.8 ±12.7) ( t=6.127, P<0.05).But no statistical significance of post-operative GCS (6.6 ±1.6 in early therapy group;6.1 ± 1.4 in late therapy group ) and discharging GOS ( 3.0 ±0.7 in early therapy group; 2.9 ±0.7 in late therapy group) was found between the two groups (t=1.822, 0.631 respectively; P>0.05).The early therapy group also presented a significant decrease of complications , such as hydrocephalus (χ2 =6.369, P<0.05 ) and venous thrombosis (χ2 =5.158, P <0.05 ), compared to the late therapy group.Furthermore, Statistical analysis revealed that therapeutic time closely correlated with the prognosis of patients with ruptured intracranial aneurysms at Hunt-Hess Ⅳ-Ⅴ levels ( P =0.009, OR =3.897).Conclusion The early stage therapy can improve the prognosis and decrease incidence of complications , which should benefit the efficient management of the patients with interventional embolization of ruptured intracranial aneurysms.

Objective To observe the effects of computer assisted training combined with the actual environment training on vascular cognitive impairment with no dementia (VCIND) after stoke.Methods Sixty elderly patients with VCIND after stroke were randomly divided into a research group and a control group,each of 30.All patients were given routine medication and rehabilitation training,while the research group was additionally provided with computer assisted training and the actual environment training lasting two months.All patients' cognitive function and activities of daily life (ADL) in both groups were assessed using the Montreal cognitive assessment scale(MoCA) and modified Barthel index(MBI) before and after treatment.Results After two months of treatment,the total score of MoCA (22.80 ± 4.63) and the scores of seven subprojects including visual space (4.00 ± 0.93),naming (2.67±0.62),attention(4.13 ±0.74),language(2.33 ±0.62),abstract thinking(1.60± 0.83),delayed memory(2.93 ±0.70) and orientation(5.13 ± 1.19) in the research group were significantly higher than those before treatment and those of the control group.However,in the control group,only the total score and the scores of naming,language and delayed memory in the control group significantly were higher than those before treatment.After treatment,the scores of MBI in both group were significantly promoted,reaching (61.53 ± 7.13) and (52.20 ± 4.93) for the research and control group respectively,with the former improving more significantly than the latter.Conclusion The computer assisted training combined with the actual environment training helps to improve cognitive function and ADL for patients with VCIND after stoke.Such combined therapy is worth of promoting in clinical practice.

Objective To research the effect of Dingxin capsule on the level of TXB2 and MMP-9 in rats, and explore the mechanism of treating and preventing atrial fibrillation (AF) by TCM. Method AF animal model was established by injecting the calcium chloride-acetylcholine mixed liquid in coccygeal vein of rats. The rats with typical ECG were divided randomly into 4 groups:AF model group (M-group), Dingxin capsule group (D-group), verapamil group (V-group), normal group (N-group). TXB2 and MMP-9 were measured 4 weeks later. Result Comparing with M-group, the level of TXB2 and MMP-9 of D-group decreased obviously (P

Objective To discuss and compare the anti-tumor effects of aspirin and celecoxib on breast cancer cell MCF-7 through investigating the effects of aspirin,celecoxib,and combined with anastrozole respectively on the growth of human breast cancer cell MCF-7.Methods The human breast cancer cell MCF-7 were treated with 2.5,5.0,and 10.0 mmol?L-1 aspirin and 30,60,and 120 ?mol?L-1 celecoxib for 24,48,and 72 h respectively,the MCF-7 without treatment with drug was used as negative control group,the MCF-7 treated by ADM was used as positive control group,the inhibitory effect was detected by MTT assay.Besides,the MCF-7 cells were treated by anastrozole(0.5 and 1.0 ?mol?L-1),anastrozole(0.5 and 1.0 ?mol?L-1)combined with aspirin(2.5,5.0,and 10.0 mmol?L-1)or celecoxib(30,60,and 120 ?mol?L-1)for 48 h,respectively,the inhibitory rate was detected by MTT assay.Results ① The inhibitory rate of the MCF-7 cell line treated with aspirin was reduced compared with controls,which was in time-dependent and dose-dependent manner(P

Objective To investigate the value of minimal invasion technique in treatment of breast benign disease.Methods 204 benign breast tumors of 74 women were completely excised by using Mammotome or VACORA.Results 108 masses were confirmed fibroadenoma,66 fibroadenosis,30 adenosis of breast.Postoperative followed up 3～12 months:there were 2 cases of local hematoma,5 cases of subcutaneously hematoma,1 case of recurrence,no infection.Conclusions There ale many advantages using uhrasound-guided minimal invasion technique to treat benign breast disease,including accurate localization,minimal invasion,less bleeding,easy performation,less complications and good cosmetology.

Objective To study CT feature of hypoxic-ischemic encephapathy in neonates and its value in predicting prognosis.Methods CT scanning was performed in 62 cases with HIE.CT feafure were analyzed.Results Of 62 cases during their neonatal period,brain edema was seen in 38 the lower density's lesions of subcortical with matter in 42,cerebral hemorrhage in 8,subarachnoid space hemorrhage in 16,SDH in 4,and intraventricular hemorrhage in 2.Of 8 cases during their infants period CT scanning at 6,12 months,dilaled ventricle was seen in 6,increased extracerebral space in 8,and periventricalar leukomalacia in 2.Conclusion CT scaning is helpful for evaluating brain injury and predicting prognosis in neonates with HIE.

Objective By using immunohistochemical staining of neuropeptide Y (NPY) and fluorescence of ethylaldehyde acid-induced biomonoamine mediators, to observe the distribution of sympathetic nerve terminals in human cervical capsule tissues, and to compare the two methods for further improvement. Methods Surgically removed joint capsule tissues from C 3~7 vertebrae of 17 cases were treated by paraffin imbedding with NPY immunohistochemical staining and frozen-section with fluorescence staining of ethylaldehyde acid-induced biomonoamine mediators. Slides were preconditioned by 1% KMnO4 and sections 15~20 ?m in thickness were made. The staining was conducted at 100 ℃ for 5 min firstly, and then at 80 ℃ for 2 min. We utilized adrenal glands of rats as positive control. The samples were observed under fluorescence microscope at 380~420 nm excitation wavelengths from a high-voltage mercury light source. Results NPY immunostaining findings indicated bulky positive materials in some arteriolar walls and nerve tracts of the joint capsules; biomonoamine mediators gave off fluorescence in green-yellow color under the induction of ethylaldehyde acid, which presented mostly as reticular or radial finely-broken fibers in vascular walls, basal laminae of the synovial membrane and dense connective tissues. The positive rates of NPY immunohistochemical staining were 70.6% (12/17) at C 3~4 intervertebral segment, 42.9% (6/14) at C 4~5 , 57.1% (8/14) at C 5~6 , and 50.0% (5/10) at C 6~7 , respectively, the total positive rate being 56.4% (31/55). When using the ethylaldehyde acid-induced biomonoamine fluorescence, the positive rates were 70.6% (12/17) at C 3~4 intervertebral segment, 93.8% (15/16) at C 4~5 , 66.7% (10/15) at C 5~6 , and 80.0% (8/10) at C 6~7 , respectively, the total positive rate being 77.6% (45/58). The positive rate was remarkably higher in ethylaldehyde acid-induced biomonoamine fluorescence than in NPY immunohistochemical staining, with statistically significant difference (?2=5.774,P=0016), especially at C 4~5 intervertebral segment (P=0.004). Conclusions Both the two methods can demonstrate the distribution of sympathetic nerve terminals, suggesting the presence of the terminals in human cervical capsule tissues. Modified ethylaldehyde acid-induced biomonoamine fluorescence offers a greater specificity.

Objective To investigate effect of tacrolimus on renal function, blood lipids, cytokines and peripheral HMGB-1 and NF-κB in nephrotic syndrome patients.Methods From January 2012 to January 2014 in the Fifth Affiliated Hospital of Zhengzhou University, 127 cases of patients with nephrotic syndrome were included based on random number table, and divided into two groups (n=65) and control group (n=62).Control group with conventional symptomatic treatment, observation group in combination with tacrolimus treatment on the basis of control group, which were treated for a total of two courses, eight weeks for a course of treatment.Serum creatinine and blood urea nitrogen levels, TG, TC, HDL-C, LDL-C levels, serum IL-1, TNF-αlevels and serum HMGB-1 and NF-κB levels were evaluated.Results The serum creatinine and blood urea nitrogen levels after treatment was significantly lower than that of control group, respectively ( P<0.05 ) .TG, TC and LDL-C levels after treatment in observation group was significantly lower than that of control group respectively (P<0.05).Serum IL-1 and TNF-αlevels after treatment in observation group was significantly lower than that of control group respectively (P<0.05).Serum HMGB-1 and NF-κB levels after treatment in observation group was significantly lower than that of control group respectively (P<0.05).Conclusion Tacrolimus can reduce blood lipid levels, inflammatory reaction and serum HMGB-1, NF-κB levels in nephrotic syndrome patients, and significantly improve renal function.

Objective To investigate effect of alprostadil on serum levels of inflammatory cytokines and nutritional biochemical indexes in patients with chronic renal failure.Methods According to the random number table, 129 patients with chronic renal failure patients were included in this group and randomly divided into observation groups (n=67) and control group (n=62).Control group were given conventional symptomatic treatment, and the observation group were given alprostadil treatment on the basis of control group with a course of four weeks of both groups.The Scr, BUN, IL-6, TNF-α, CRP, Ca2 +, ALB, P3 +, Hb, RBC, hemorheology changes were compared before and after treatment.Results The Scr, BUN levels after treatment was significantly lower than before treatment (P<0.05), and Scr, BUN levels in observation group after treatment was significantly lower than control group (P<0.05).Serum levels of IL-6, TNF-α, CRP after treatment significantly decreased than before treatment (P<0.05), serum IL-6, TNF-α, CRP after treatment was significantly lower than control group ( P<0.05 ).After treatment, Ca2 +, ALB levels of observation group was significantly higher than control group and P3 +was significantly lower than control group (P<0.05), while there were no significant differences of Hb, RBC.The hemorheology of observation group before treatment was significantly lower than control group ( P<0.05 ) .Conclusion Alprostadil can significantly improve renal function, and the possible mechanism may be associated with reducing serum levels of inflammatory factors and improving blood rheology, which delay progression of chronic renal failure effectively.

Objective The relationship between XbaI polymorphism of ApoB gene and cerebral infarction(CI)among Chinese population was assessed by Meta-analysis.Methods All related case-control studies were collected from all publications,the relevant studies were identified after eliminating those poor-qualified studies.Meta-analysis was conducted for investigating heterogeneity among individual studies,and summarizing the effects across studies.Results The combined data statistics revealed the frequencies of the X-X+/X+X+ genotypes showed no statistically difference(Z=1.72,P=0.08).Through the subgroup analysis,it was obviously increase the risk of CI in Chinese northern population(OR=2.66,95%CI:1.67~4.24),but no statistically difference in Chinese southern population(OR=1.26,95%CI:0.90~1.77).Conclusions ApoB gene XbaI polymorphism may be significantly associated with susceptibility of CI in Chinese northern population,but not a definite risk for Chinese southern population.