Objective To determine the status of self-efficacy and self-care agency of the empty nest elderly patients in the community,and analyze their correlation. Methods A total of 320 empty nest elderly with chronic disease in Fushun, Liaoning Province. The General Information Questionnaire, Exercise of Self-Care Agency scale (ESCA) and the Chronic Disease Self-efficacy Scale were used to investigate the empty nest elderly with chronic disease in the community and analyze the present situation level of the self-care agency and the self-efficacy and the correlation between them. Results General data showed no significant difference between solitary empty-nest elderly and connubial empty-nest elderly (P>0.05). Solitary empty-nest elderly got (93.36 ± 13.49) points and (5.22 ± 0.62) points in ESCA and the Chronic Disease Self-efficacy Scale,which were lower than (98.10±11.34) points and (5.32±0.30) points of connubial empty-nest elderly, the difference was statistically significant (t=-0.866,-0.467, P<0.05). There was significantly positive correlation between self-efficacy and self-care agency (r=0.318, P<0.01). Conclusions There is positive correlation between self-efficacy and self-care agency. It is suggested to implement community-based nursing interventions to enhance their self-efficacy and to promote their self-care agency,there by to improve their quality of life .

Echocardiography, Three-Dimensional ; Heart Defects, Congenital ; diagnostic imaging ; therapy ; Humans

Establishment of bioassay methods is the technical issues to be faced with in the bioassay of Chinese materia medica. Taking the bioassay of Coptis chinensis Franch. as an example, the establishment process and application of the bioassay methods (including bio-potency and bio-activity fingerprint) were explained from the aspects of methodology, principle of selection, experimental design, method confirmation and data analysis. The common technologies were extracted and formed with the above aspects, so as to provide technical support for constructing pattern and method of the quality control for Chinese materia medica based on the dao-di herbs and bioassay.

Objective　To study the alterations of surfactant protein A (SP-A) and B (SP-B) in rats in the early stage of acute lung injury induced by oleic acid. Methods　An acute lung injury model was established by injecting oleic acid into the superior vena cava of rats and the levels of SP-A and SP-B in branchoalveolar lavage fluid (BALF) were determined with Western Blot and ELISA. Results　The levels of SP-A and SP-B in BALF were significantly decreased in the injured group 1 h after acute lung injury (P<0.01) and remained low in the next 6 h. Conclusion　The levels of SP-A and SP-B were decreased in the early stage of acute lung injury. The methods used for detecting SP-A and SP-B we adopted are specific and sensitive, and might be helpful for other experimental study.

Epilepsy, Absence ; Humans

Objective To study the effect and mechanism of gene PBP2 and gene PBP3 in drug resistance of Klebsiella pneumoniae(KP) to different antibacterial agents.Methods A total of 203 strains of KP which isolated from clinical samples in each department in our hospital during the period from April 2014 to April 2015.Strains were identified and antibiotic susceptibility were determined with automatic bacteria identification system,then extracted DNA genome from bacteria,proceeded with electro-phoresis after the product of PBP2 and PBP3 were amplified by PCR,examined genetic expression with RT-PCR,and study their connection to drug-fast of the KP combining results of antibiotic susceptibility.Results In 203 strains of KP,105 strains resistant to β-lactam drug (51.7%),88 strains were sensitive,resistant rate to imipenem and meropenem were 5.4%,3.9% respectively.The PCR amplification products of the electrophoresis bands of PBP2,PBP3 gene,18 strains were negative,185 strains were positive,which was not parallel,there were respectively negative and positive strains among them.There was no significant correlation between PBP2,PBP3 and multiple drug resistance of KP(P=0.295,0.628).PBP2 was not related to the resistance of carbapenem(P=0.637).PBP3 had correlation with carbapenem resistance,but the correlation coefficient was not high(P=0.041,r=0.148).After reverse transcription of PBP2,PBP3,statistically analyze electrophoresis bands of amplification products of carbapenem-resistant KP and 16SrRNA amplification bands of gray gradient ratio,there was no statistical significance(PBP2:P=0.331,PBP3:P=0.383).Conclusion Gene PBP2,PBP3 might not be the necessary reasons of KP being multiple drugs resistant.

Objective To investigate the association between systemic lupus erythematosus(SLE) and polymorphism of cytotoxic T-lymphocyte antigen 4 gene(CTLA-4) and the effect on ANA and anti-dsDNA antibody.Methods The A/G substitution was investigated in the exon 1 position 49 in 92 SLE patients and 60 controls by PCR restriction fragment length polymorphism(PCR-RFLP).ANA and anti-dsDNA antibody were examined by indirect fluorescence immunoassay.Results SLE patients had significantly higher frequencies of the CTLA-4+49GG allele than controls(P

Principles of constructing medical information technology curriculum system were pro-posed revolving round the core problem of how to combine needs of developing life science with culturing talents who can independently apply information technology knowledge in studying and researching. De-tailed implementing measures for constructing medical information technology curriculum system were summarized after repeated practical teaching. Longitudinal classification based on discipline and trans-verse classification based on teaching level were combined to construct the whole standard course. Finally, courses were appropriately distributed according to detailed culturing objectives for students in medical universities and colleges,thus a suitable medical information technology curriculum system in medical universities and colleges was formed.

Objective To understand the types and risk factors of human papillomavirus (HPV ) infection among the patients with cervical intraepithelial neoplasia in Chongqing to provide a scientific basis for prevention and treatment of cervical cancer . Methods The patients with TCT screening in many hospital of Chongqing from June 2012 to December 2013 were selected and performed the HPV typing ,9 328 cases succeeded in HPV typing .The main types of HPV infection in different grades of cervical intraepithelial neoplasia were statistically analyzed for determining the risk factors .Results HPV16 was the most common infection type among patients with cervical intraepithelial neoplasia in Chongqing area ,followed by HPV52 ,58 ,33 ,18 .Some factors such as multiple sex partners ,young female with sexual debut and induced abortion were the risk factors of HPV infection .Conclusion Single and high-risk type is the main feature of HPV infection among the patients with cervical intraepithelial neoplasia in Chongqing .The infection rate is higher with the higher grade of cervical intraepithelial neoplasia .Publicity and education for risk factors of HPV infection should be strengthened ,which will help to prevent the occurrence of cervical cancer .