Objective: To investigate the influence of ischemic and pinacidil preconditioning on the activity of NF-?B and its myocardial protective effects.Methods: Eighty isolated rabbit hearts were randomized into five groups(Group C?K?I?P?G).Hearts were removed and mounted in a Langendorff apparatus perfused with 37℃ oxygenated Krebs-Henselet buffer.All groups were subjected to gobal myocardial ischemia for 40 min and reperfusion for 60 min or 120 min.Cardiac functions were monitored at 10 min equilibration ?15 min?30 min?60 min and 120 min reperfusion;Coronary flow(CF) was measured at 10 min equilibration and 30 min reperfusion;Expression of NF-?B P65 was determined at 10 min equilibration?60 min and 120min reperfusion.Results:(1)After ischemic reperfusion,the recovery of cardiac function and CF in Group I and Group P was significantly superior to that in Group C and Group K(P

This paper reports the situation of the treatment for stroke and introduces stroke unit (SU) in overseas hospitals : its important role in the emergency treatment and rehabilitation, the characteristics, advantages and managing modes. Moreover,it discusses the possibility and necessity of establishing SU with characteristics of traditional Chinese medicine.

[Objective]To establish a scoring standard of operative risk assessment for the orthopaedic surgery and evaluate the reliability of it.[Method]Anivariable analysis was performed for the preoperative and intraoperative clinical data of 768 patients undergoing orthopaedic surgery from Jan.2001 to Dec.2008,the risk factors associated with orthopaedic surgery were selected.Then multivariable analysis with logistic regression was performed for the selected risk factors.The scoring standard was made through these data.Reliability of the standard was evaluated by predicting of 160 patients in spinal surgery from 2006 to 2008,comparing with the real situation of each operation.[Result]Anivariable analysis showed age,heart function,blood pressure,electrocardiogram,sternum,liver function,renal function,albumin,blood sugar,number of operative variables within four weeks,magnitude,blood loss preoperation,timing of operation were risk factors.[Conclusion]The ORAOS could appropriately predict the occurrence of patients' postoperative complication before operation.

To discuss long-term changes of refractive status in children after congenital cataract intraocular lens ( lOL) implantation and analyze its related influence factors.METHODS: Seventy cases ( 110 eyes ) with congenital cataract from January 2013 to January 2014 in our hospital were selected, according to the age of patients, they were divided into group A (<2 years old), group B (2~6 years old) , group C ( 7 ~ 12 years old ) . They were all given cataract extraction combined with lOL implantation in congenital cataract. Corneal curvature, axial length, refractive status of three groups after operation were compared, the influence factors ocular axis changes were analyzed. RESULTS: There were no statistically significant differences in corneal curvature of groups A, B, and C after operation ( P>0. 05 );The differences had statistical significances of the corneal curvature of group A after surgery and 3mo after operation (P<0. 05), there were no significant differences of the corneal curvature of group B, group C after surgery and 3mo after operation (P>0. 05);the corneal curvature 3mo after operation of group A was significantly higher than that of groups B and C (P<0. 05), there were no significant differences in the corneal curvature between group B, and group C after 3mo ( P>0. 05 ); There were no statistical significances on axial length after operation in groups A, B, and C (P>0. 05);Ocular axial length difference of group A 3mo after surgery was significantly higher than that of groups B and C ( P < 0. 05 ), ocular axial length had no statistical difference of group B, group c 3mo after surgery ( P>0. 05). Refractive status of three groups after operation showed myopia moving trend, refractive change rate and refractive diopter of group A after operation was significantly higher than that of group B, group C, the difference was statistically significant (P<0. 05). CONCLUSlON: After congenital cataract extraction combined with lOL implantation, patients present myopia refractive state trend. Performance of children below 2 years old is significant. The main factor of myopia is axial growth.

With the intense development of neoadjuvant chemotherapy for breast cancer research in recent years, this approach has been used widely in clinical treatment. Neoadjuvant chemotherapy can increase the chance of operation for patients with local y advanced breast cancer. Moreover, it can improve breast-conserving rate in larger-tumor patients, thereby improving the quality of life of these patients. However, some controversies regarding the use of neoadjuvant chemotherapy for breast cancer patients remain to be resolved. This review focuses on clinical studies and re-recognition of neoadjuvant chemotherapy as a viable treatment for breast cancer.

Objective Central nervous system trauma, ischemia, hemorrhagic stroke were all experienced the same pathological damage, in related animal experiments,histone deacetylase inhibitors have a wide range of neuroprotective effect, it is expected to become a new class of potential therapeutic agents.However,the specific mechanism is not clear.In this paper, the research progress of neuroprotective effect of histone deacetylase inhibitors is reviewed,in order to improve recognition of histone deacetylase inhibitors and provide foundation for futher study.

Objective To analyze the diagnose and treatment of drug-induced hypersensitivity syndrome (DIHS) in children.MethodsThe clinical data from one case of highly suspected DIHS were retrospectively analyzed. The related literatures were reviewed.ResultsA 22-month-old male child with severe pneumonia, after treated with vancomycin, suffered with high fever and skin rash combined with hepatic lesion and hematological system disorder. After withdrawal of vancomycin and then treatment with the combination of high-dose methylprednisolone and intravenous immunoglobulin, the clinical symptoms were gradually relieved in 24 hours. The child was improved and discharged on 17th day.ConclusionThe child has the characteristics of DIHS which is highly suspected to be caused by vancomycin.

Objective To explore the effect of creatine phosphate combined with salvia miltiorrhiza polyphe-nols acid salt in treatment of coronary heart disease heart failure.Methods 114 cases of patients with coronary heart disease heart failure were selected as research objectives,who were treated in our hospital from August 2012 to August 2014.And they were randomly divided into control group(55 cases)and research group(59 cases)according to the number table method.Routine therapy of heart failure were given in both groups and the research group were added creatine phosphate combined with salvia miltiorrhiza polyphenols acid salt.The clinical efficacy of two groups of patients were observed and compared.Results The total effective rate in research group was 96.61%(57 /59), significantly higher than 85.45%(47 /55)in control group,the difference was statistically significant(χ2 =4.427, P =0.035).Before treatment,the left ventricular ejection fraction(LVEF),left ventricular end diastolic diameter (LVEDD),left ventricular end systolic diameter(LVESD)and 6min walking distance between two groups had no sig-nificant difference(P >0.05).After treatment,the LVEDD and LVESD of research group were decreased and LVEF and 6 minutes walking distance of two groups were increased,the difference was statistically significant(P <0.05), the LVEDD and LVESD of research group were lower than that of the control group and LVEF and 6 minutes walking distance were higher than that of control group,the difference was statistically significant(P <0.05).No complications or side effects related to the treatment were found in both groups.Conclusion On the basis of routine therapy of heart failure,the effect of creatine phosphate sodium combined with salvia miltiorrhiza polyphenols acid salt in treat-ment of coronary heart disease heart failure can improve cardiac function of the patients with the indicators,and the clinical effect is obvious,and side effects is rarely observed.

BACKGROUND:Bone morphogenetic protein 9 is proved to promote the osteogenic differentiation of various kinds of stem cel s, but whether it can induce the osteogenic differentiation of dental fol icle cel s in vitro is yet unclear. OBJECTIVE:To investigate whether bone morphogenetic protein 9 can induce the osteogenic differentiation of rat dental fol icle cel s in vitro. METHODS:Purified rat dental fol icle cel s at passage 3 were transfected with bone morphogenetic protein 9 adenovirus. Then, alkaline phosphatase activity, calcium deposition and expression of osteogenesis-related factors at mRNA and protein levels were detected in the dental fol icle cel s. RESULTS AND CONCLUSION:After transfection with bone morphogenetic protein 9, the dental fol icle cel s showed continuously enhanced alkaline phosphatase activities and obviously enhanced calcium deposition. Real-time PCR results demonstrated that the mRNA expressions of alkaline phosphatase, osteocalcin, bone sialoprotein, osteopontin and core binding factor were increased significantly. The western blot assay showed that the expression of osteopontin enhanced in the dental fol icle cel s after transfection with bone morphogenetic protein 9. In summary, bone morphogenetic protein 9 can induce the osteogenic differentiation of dental fol icle cel s.

Objective To observe the clinical efficacy of auricular point sticking plus moxibustion at Zusanli (ST 36) in preventing spleen-stomach disharmony due to intravenous analgesia pump.Method Totally 120 post-operation patients with intravenous analgesia pump were randomized into a treatment group and a control group, 60 in each group. Patients in the control group were asked to take a semireclining position, and to turn over the body and do morning exercise under assistance. In addition to the intervention given to the control group, the treatment group received auricular point sticking plus moxibustion at Zusanli (ST 36). The occurrence rate of post-operation nausea, vomiting and abdominal bloating was observed.Result The occurrence rate of nausea, vomiting and abdominal bloating was 11.7% in the treatment group, versus 33.3% in the control group, and the difference was statistically significant (P<0.05).Conclusion Auricular point sticking plus moxibustion at Zusanli (ST 36) is effective in preventing spleen-stomach disharmony due to intravenous analgesia pump, and can reduce pain and complications.