Objective:To study the surface enhanced Raman scattering ( SERS) of safflower and identify safflower injections by SERS quickly and effectively. Methods:Through comparative analysis of the Raman spectroscopy of safflower injections and the corre-sponding control herbs, the rapid identification of safflower injections was realized. Results:The results showed that several character-istic peaks of safflower were enhanced obviously in SERS, which could be used to identify safflower injections. Conclusion:The meth-od is reliable, rapid, accurate and specific, which can be applied as a method to identify safflower and its injections.

Venous thrombotic disease is a serious disease,which impact on health and life-threatening.Pulmonary embolism and deep-vein thrombosis are the two components of a single disease called venous thromboembolism in obstetrics and gynecology.but it can be difficult to diagnose because clinical symptoms and signs are non-specific or absent in early venous thrombus embolism (VTE).It has great value that how to use the most economic,simple,efficient method for screened high-risk groups,timely and accurate laboratory diagnosis of VTE.

ObjectiveTo develop the military acute stress scale and examine the reliability and validity of this scale.MethodsBased upon the previous acute stress researches and the rule of psychometrics,the military acute stress scale was compiled according to open-ended inventory survey.A total of 630 personnel were chosen by random cluster sampling,and tested with this scale,50 of them were tested by Chinese Military psychosomatic health Scale simultaneously,and 50 of them were retested after two week.The reliability and validity were examined by exploratory factor analysis,confirmatory factor analysis and correlation analysis.ResultsThe military acute stress scale was comprised of 9 factors and one lie sub-scale ; Main fit indexes of model were the following CMIN =1219.476,DF =593,P =0.000,CMIN/DF =2.056,IFI =0.927,TLI =0.902,CFI =0.924,RMSEA =0.059; The conrelation coefficients between subscales and subscales were 0.284-0.572.The correlation coefficients between subscales and total scale were 0.700 ～ 0.846 (P ＜ 0.05 or 0.01 ) ; The correlation coefficients was significant between this scale and Chinese military psychosomatic health scale.The correlation coefficients were 0.338-0.962 (P ＜ 0.01 ).The test-retest correlation coefficients of total scale and subscales were 0.379-0.909 (P ＜ 0.01 ).The Cronbaeh's α coefficients were 0.606-0.926 ; and the split-half correlation coefficients were 0.466-0.855.ConclusionThe military acute stress scale has acceptable psychometrics properties on reliability and validity.

Objective:To study the application value of Belinson biopsy forceps and traditional biopsy forceps in cervical intraepithelial neoplasia. Methods:Two hundred and fourty cases of cervical intraepithelial neoplasia patients received cervical biopsy under colposcopy from 2011 August to 2014 March were enrolled and randomly divided into observation group received Belinson biopsy forceps biopsy and control group received conventional biopsy forcep. Then coincidence rate between cervical biopsy and final pathological diagnosis, biopsy condition and stress levels of two groups were compared. Results:Coincidence rate between cervical biopsy and final pathological diagnosis of observation group was higher than that of control group;biopsy tissue volume, vaginal bleeding amount were less than those of the control group; pain scores after biopsy was lower than control group and cervical repair time was shorter than control group. Serum ACTH, Cor, NE levels were lower than control group. Conclusion:Belinson biopsy forceps biopsy is helpful to improve the accuracy of diagnosis, control the degree of tissue trauma and stress reaction degree, promote cervical repair and reduce local bleeding after biopsy;it’s an ideal method in biopsy of cervical intraepithelial neoplasia.

Objective To detect the effects of chemotherapy on the immune function of patients with ovarian cancer.Methods Thirty-four patients with ovarian cancer(ovarian cancer group)who accepted chemotherapy were collected.Thirty healthy women were used as control group.The lymphocyte sub-cluster CD3+,CD4+,CD8+ and CD25+of peripheral blood were detected by flow cytometry.Results The peripheral blood CD3+,CD4+ and CD4+/CD8+ in ovarian cancer group were lower than those in control group(0.5524±0.1121 vs.0.6685±0.0869,0.3183±0.0864 vs.0.4139±0.0524,1.06±0.29 vs.1.43±0.21),CD4+ CD25+was higher than that in control group(0.1026±0.0418 vs.0.0287±0.0132),there was significant difference between two groups(P<0.05).There was no significant changes observed 1 week after chemotherapy(P>0.05).Three weeks after chemotherapy,the CD3+,CD4+ and CD4+/CD8+ in ovarian cancer group were higher and CD4+ CD25+ was lower,there were significant difference between two groups(P<0.05).Conclusions The ratio of lymphocyte sub-cluster is imbalance in the patients with ovarian cancer.Chemotherapy can improve the immunosuppression condition and recovery the ratio of lymphocyte sub-cluster in the patients with ovarian cancer.

Objective To observe the effect and safety of different doses of sufentanil plus subarachnoid block during labor. Methods One hundred parturients (single birth) were divided into 5groups by random digits table with 20 cases in each, which sufentanil 3,4,5,6,7 μ g in group A, B, C, D, E plus ropivacaine 3 mg were injected respectively into subarachnoid space. The onset and duration of subarachnoid analgesia were observed; visual analogue scale (VAS) were recorded respectively before injection and 5,15,30,60 min after injection; the vital sign,labor stage,labor type,adverse reactions of parturients,fetal heart rate (FHR) and the Apgar score at 1,5 min of neonate were recorded respectively.Results The onset of subarachnoid analgesia in group A was higher than that in group B,C, D,E;the duration of subarachnoid analgesia in group A,B was shorter than that in group C,D,E.VAS declined gradually 5,15,30,60 min after injection compared with before injection in 5 groups (P ＜ 0.01 );VAS in group B, C, D, E was lower than that in group A at 5 min after injection (P ＜ 0.05 ), and VAS in group C, D, E was lower than that in group A,B at 60 min after injection (P＜ 0.05). There was no significant difference in vital sign,labor stage,labor type,FHR and the Apgar score of neonate among 5 groups (P ＞0.05). The occurrence of itch of skin and nausea in group D,E [25%(5/20), 15%(3/20) in group D,and 40%(8/20),20%(4/20) in group E] was higher than that in group A, B, C [5%(1/20), 0 in group A, 10%(2/20), 0 in group B, 10% (2/20), 0 in group C] (P ＜ 0.05 ). Conclusion Subarachnoid block (sufentanil 4-6 μ g +ropivacaine 3 mg) can offer safety and efficacy of labor analgesia; sufentanil 5 μg is the best dose with subarachnoid block during labor at present.

Objective To investigate the self-efficacy and other factors of the internship and discuss the effect of the self-efficacy in medical education.Methods Using the Face Scale,Trait Coping Style Questionnaire,General Self-efficacy Scale to measure the level of self-efficacy and other factors.Results Correlation was found between self-efficacy and other factors such as subjective well-being,positive coping style and professional stress.Conclusion The self-efficacy and other factors impact each other and reduce the professional satisfaction.

Objective:To observe the effect of intrapulpal injection in the pulpectomy of primary molars.Methods:22 petients with 44 diseased primary molars were divided into 2 groups(n =22 teeth).The intrapulpal injection with a computer-controlled local anesthetic delivery system STATM was performed in the experimental Group,and traditional infiltration anesthesia technique was conducted in the control group.Results:During the anesthesia process,the VAS value of the experimental group was lower than the control group(P ＜ 0.05).The pain control in the experimental group was better during pulp removal within 1 min.There was no occurrence of postoperative soft tissue numbness and factitial ulcer in the experinental group.Conclusion:The intrapulpal injection,administered with STATM,is more effective than traditional infiltration anesthesia in pulpectomy of primary molar teeth.

Objective: To establish an HPLC method for the determination of gentiopicroside,gardenia and baicalin in Xiegan Anshen pills.Methods: The analysis was carried out on a Phenomenex Luna C18(2) column(250 mm×60 mm, 5 μm).The mobile phase consisted of methanol-0.2% phosphoric acid solution with gradient elution and the flow rate was 1.0 ml·min-1.The column temperature was 30℃, the detection wavelength was 254nm, and the sample size was 10 μl.Results: The linear range of gentiopicroside was 10.55-168.80 μg·ml-1 (r=0.999 9),and the average recovery was 98.68%(RSD=1.2%, n=6).The linear range of gardenia was 29.85-477.60 μg·ml-1 (r=0.999 8), and the average recovery was 98.76%(RSD=1.1%, n=6).The linear range of baicalin was 43.05-688.8 μg·ml-1 (r=0.999 7), and the average recovery was 98.36%(RSD=1.4%, n=6).Conclusion: The method is simple and accurate, and can determine the contents of the three ingredients simultaneously, which can be used for the quality control of Xiegan Anshen pills.

Objective To evaluate the effect of pelvic adhesiolysis to prevent readhesion in the second look operation.Methods 30 rats underwent laparotomy incision of uterus to create intraperitoneal adhesions models.The animals were randomly divided into three groups:the first group:isotonic Na chloride (n =10);the second group:medical Sodium Hyaluronate (n =10),the third group:Oxidized regenerated cellulose.After 2-week recovery period,the animals were operated andintraperitoneal adhesions were assessed,then,all adhesions were released and set isotonic Na chloride,medical Sodium Hyaluronate and Oxidized regenerated cellulose.Again,After 2-week recovery period,the animals were killed and the intraperitoneal adhesions were assessed.Results After the first operation the three groups had significant difference (F=7.114,P＜0.05),After the second operation the three groups had no significant difference(F=0.280,P＞0.05).Conclusion Medical sodium hyaluronate and oxidized regenerated cellulose are effective methods to prevent postoperative adhesions.However,they are ineffective to prevent readhesion after adhesiolysis.