5srRNA was isolated and purified from the reticulocytes of rabbit. It labeled with ~(125)I, then incubated with mouse myeloma cells (SP2/0). By autoradiography it was observed that ~(125)I-SsrRNA could pass into the nuclei of the cells. In a separate experiment, it showed that the incorporation rate of ~3H-TdR into nuclei of SP2/0 after incubation with 5srRNA was decreased as compared with that f control group, hence the result indicates that 5srRNA inhibits DNA synthesis of the SP2/0 cells and it seems to play a role in the regulation of gene expression through its hybridization with DNA sequences of the SP2/0 cells. Thus it is likely that 5srRNA might act as "erythroid denucleation factor".

Administration, Inhalation ; Animals ; Erythrocyte Count ; Erythrocytes ; drug effects ; Female ; Formaldehyde ; toxicity ; Hemoglobins ; analysis ; Male ; Mice

Objective: To investigate the relationship between the expression of Raf-1 and tumor angiogenesis,and to discuss its clinical significance.Methods: Tissue microarray technique was used to detect the expression of Raf-1 in 87 specimens of human colon carcinoma,their corresponding adjacent tissues,and incision margins.The patients were from the Department of Pathology of Xijing Hospital between 2005 and 2006.Microvessel density(MVD) was detected using immunohistochemistry with CD34 labeling.The correlation between Raf-1 expression and MVD with the tumor size,metastasis,and differentiation was analyzed.Results: The positive rates of Raf-1 in colon carcinoma tissues,adjacent tissues and incision margins were 86.47%,37.34% and 11.03%,respectively;there were significant difference among the 3 values(P

Objective To study the genetic relationship among Dioscorea zingiberensis with different diosgenin content at DNA level.Methods DNA of all types was amplified by 12 random primers from 40 random primers,the polymorphic bands of RAPD were counted.And the results of types were analysed by POPGENE 1.31(Tool for Population Genetic Analysis 1.31) software.Results A total of 73 bands was obtained,64 of total bands(87.67%) was polymorphic.The results indicated that there were apparent and abundant genetic variations in D.zingiberensis. Conclusion The formation and accumulation of diosgenin could be affected significantly by the genetic basis of D.zingiberensis.

OBJECTIVE: To study the antimicrobial effect of 11 kinds of oral Chinese patent medicines and to establish microbial limit test method for them. METHODS: Common method and culture medium dilution method and sticked filter membrane method were used to do the recovery test in order to validate the scientificity and feasibility of established method. RESULTS: Danqi piantan capsule showed strong inhibition effect on Staphylococcus aureus and Bacillus subtilis. Xinshuning tablet, Fuzheng mixture, Shenkangning mixture, Qingshen xiaodu oral liquid had antimicrobial effect on Staphylococcus aureus to some extent. Banlangen granule had antimicrobial effect on Bacillus subtilis. The effect can be eliminated by culture medium dilution method. CONCLUSION: The common method can be used for the microbial limit test of Jiaoou capsule, Zhike ningsou capsule, Rongdanshi granule, Sanjie mingmu capsule, Jiangzhi ruanmai tablet. Dilution method can be used for the microbial limit test of Xinshuning tablet, Fuzheng mixture, Shenkangning mixture, Qingshen xiaodu oral liquid, Banlangen granule, Danqi piantan capsule. The common method is suitable for all kinds of specified microorganisms.

Objective The double-cavity balloon oppressor surgery for rhegmatogenous retinal detachment (RRD) has been utilized in past decades. But we have not yet a domestic device available. Present study was to investigate the effectiveness of double-cavity balloon oppressor using a domestic device in primary RRD surgery. Methods This clinical trial was a prospective study. Total 74 cases of patients with rhegmatogenous retinal detachment(RRD) (74 eyes) were enrolled. Double-cavity balloon oppressor surgery was performed in 40 eyes of 40 patients, and conventional segmental scleral buckling surgery was used in 34 eyes of 34 patients. The mean operative time, retinal attachment rate, vision and astigmatism change after operation between two different operative fashions were compared. The intra- and post-operative complication was discussed. The mean follow-up period was 4. 5 months. Written informed consent was obtained from all the patients before clinical trial. Results The retinal anatomic attachment rate was 92. 5% (37/40) in balloon oppressor surgery group and 91. 1% (31/34) in conventional surgery group, and there were no significant difference in retinal attachment rate among PVR grade A, B and C1 (P ＞0. 05) . The postoperative best corrected visual acuity (operative 3 months) was improved in 29 eyes(72. 5%) after balloon oppressor surgery and 25 eyes(73. 5%) after conventional surgery, showing insignificant difference between them (P ＞ 0. 05) . The mean surgical time was about (27. 00 ±8. 60) minutes in balloon oppressor surgery group and (47. 00 ± 14. 30) minutes in conventional surgery group(t =7. 41, P ＜0. 01) . Cylinder diopter change after surgery was insignificantly different between before and after operation in balloon oppressor surgery group (0. 52 ± 0. 30 D vs 0. 81 ± 0. 41 D, P ＞ 0. 05), however, there was a significant difference in cylinder diopter change between preoperation and postoperation (0. 65 ± 0. 32 D vs 1. 83 ± 0. 69 D, P ＜ 0. 05) . Conclusion Double-cavity balloon oppressor surgery for RRD is accurate and reliable. This operation procedure offer an economic, efficient and convenient choice for treatment of RRD.

Abstract: Objective ， To explore the effects of lead exposure on copper level copper transporter protein expression and Methods oxidative stress in mouse cerebral cortex. The specific pathogen free adult male C57BL/6 mice were randomly ， - - - divided into control group low lead exposure group and high lead exposure group with 10 mice in each group. The mice in low - ， and high lead exposure groups were respectively given 250 and 500 mg/L lead acetate in drinking water every day and the mice - ， in the control group were given double distilled water for 12 weeks. Twenty four hours after exposure Morris water maze and ， elevated cross maze were used to test the neurobehavioral function of mice. The cerebral cortex of mice was isolated and the levels of lead and copper were detected by inductively coupled plasma mass spectrometry. The activities of glutathione （ - ）， （ ） （ ） peroxidase GSH Px catalase CAT and malondialdehyde MDA were detected by histochemical method. The relative （ ） ， ， expression levels of copper transporter such as synthesis of cytochrome C oxidase SCO 1 SCO 2 and cytochrome C oxidase （ ） Results - - assembly protein 11 COX11 were detected by western blot. The escape latencies of mice in the low and high lead （ P ）， ， - exposure groups were prolonged all <0.05 while the number of crossing the platform the percentage of open arm entry - （ P ） times and the percentage of open arm retention time decreased all <0.05 compared with the control group. Mice in both the - - （ P ）， - low and high lead exposure groups increased levels of lead and copper in the cerebral cortex all <0.05 decreased GSH Px （ P ）， （ P ） and CAT activity all <0.05 and increased SCO1 relative expression all <0.05 compared with the control group. Mice in - （P ）， - the high lead exposure group showed prolonged escape latency <0.05 reduced GSH Px and CAT activities in the cerebral （ P ）， （ P ） - cortex all <0.05 increased MDA level and relative expression of SCO1 and SCO2 all <0.05 compared to mice in the low Conclusion - lead exposure group. Lead exposure increased the expression of copper and copper transport related proteins in mouse cerebral cortex and induced oxidative stress leading to central nervous system damage resulting in neurobehavioral abnormalities in mice.

Animals ; Drugs, Chinese Herbal ; pharmacology ; Medicine, Chinese Traditional ; Mice ; Microscopy, Electron ; Sputum ; drug effects

Action Potentials ; Animals ; Female ; Heart ; physiology ; Male ; Membrane Transport Proteins ; genetics ; Mice ; Mice, Knockout ; Myocardium ; metabolism ; Patch-Clamp Techniques ; Sodium Channels ; physiology

Aged ; Anthracosis ; complications ; metabolism ; pathology ; Cyclin-Dependent Kinase Inhibitor p16 ; metabolism ; Humans ; Lung ; metabolism ; pathology ; Lung Neoplasms ; complications ; metabolism ; pathology ; Male ; Middle Aged ; Retinoblastoma Protein ; metabolism