1.Effects of 1,25-dihydroxyvitamin D_3 on regulatory T cells and airway inflammation in asthmatic rats
yan, ZHOU ; xin, ZHOU ; xiao-qiu, WANG
Journal of Shanghai Jiaotong University(Medical Science) 2006;0(07):-
Objective To investigate the effects of 1,25-dihydroxyvitamin D3[1,25(OH)2D3] on regulatory T cells(CD4+CD25+ Foxp3+ T cell)and airway inflammation in asthmatic rats. Methods Asthmatic models were established in 28 rats with ovalbumin as sensitinogen,and four groups were randomly divided(n=7).1,25(OH)2D3 was orally administered 1 h before challenge in treatment group 1,dexamethasone was subcutaneously injected 1 h before challenge in treatment group 2,both 1,25(OH)2D3 and dexamethasone were given 1 h before challenge in treatment group 3,while no medicine was administered 1 h before challenge in group 4(asthmatic control group).The levels of IL-4 and IL-10 in bronchoalveolar lavage fluid(BALF) were detected,the total cell numbers were obtained and the eosinophil count was performed.The ratios of Treg to CD4+ T cells in peripheral blood and spleen mononuclear cells were measured with flow cytometry,and the mRNA expression of IL-10 and Foxp3 in lung tissues was detected.Wistar rats without asthma were served as normal controls(n=7). Results There were significant differences between asthmatic control group and normal control group in each parameter.Compared with asthmatic control group,the total cell number and eosinophil count decreased,the level of IL-4 decreased and the level of IL-10 increased in BALF in treatment groups.The ratios of Treg to CD4+ T cells in peripheral blood and spleen mononuclear cells significantly increased,and the mRNA expression of IL-10 and Foxp3 in lung tissues increased in treatment groups compared with asthmatic control group.There was no significant difference between treatment groups and normal control group in each parameter. Conclusion Administration of 1,25(OH)2D3 increases the proportion of Treg and expression of Foxp3 mRNA in peripheral blood and spleen in asthmatic rats.1,25(OH)2D3 has a regulatory effect on Treg,and may serve as a potential therapeutic approach against asthma.
2.Association of serum 25-hydroxyvitamin D3 and urine albumin-to-creatinine ratio in type 2 diabetic patients
Chinese Journal of Endocrinology and Metabolism 2012;28(4):298-300
To study the association of serum 25-hydroxyvitamin D3 level with urine albumin-to-ereatinine ratio in 193 type 2 diabetic patients.Multivariate linear regression demonstrated that serum 25-hydroxyvitamin D3 level was an independent risk factor in predicting urine albumin excretion level in type 2 diabetic patients(β =-0.02,95%CI -0.04 - -0.01,P<0.01 ).
3.Relationship between homocysteine,C-reactive protein and type 2 diabetes peripheral vascular disease
Hong YIN ; Yan XIN ; Biao ZHOU
Chinese Journal of Postgraduates of Medicine 2014;37(1):4-6
Objective To observe the relationship between homocysteine (Hcy),C-reactive protein (CRP) and type 2 diabetes peripheral vascular disease (PVD).Methods Fifty-eight patients with type 2 diabetes according to the condition of PVD were divided into simple type 2 diabetes mellitus group (DM group,30 cases) and PVD group (28 cases).Twenty-five healthy people were chosen at the same period as control group.The fasting blood glucose (FBG),glycosylated hemoglobin (HbA1c),total cholesterol(TC),triglyceride (TG),high-density lipoprotein cholesterol (HDL-C) and low-density lipoprotein cholesterol (LDL-C),Hcy and CRP levels were detected among 3 groups,and the body mass index (BMI),ankle brachial index(ABI) was calculated.Results The BMI,TG,TC and HDL-C levels among 3 groups had no statistical significance (P >0.05).The FBG,HbA1c and LDL-C in DM group and PVD group were significantly higher than those in control group [(7.54 ± 1.25),(8.12 ± 1.57) mmol/L vs.(4.68 ±0.54)mmol/L; (6.92 ± 0.89)%,(7.54 ± 0.97)% vs.(4.80 ± 0.54)% ; (3.79 ± 0.73),(4.41 ± 0.93) mmol/L vs.(2.68 ± 0.54) mmol/L],and the difference was statistically significant (P < 0.05).But the difference between PVD group and DM group had no statistical significance (P > 0.05).Hcy and CRP in DM group and PVD group were higher than those in control group [(10.26 ± 3.62),(19.83 ± 5.54) μ mol/L vs.(8.92 ± 2.41)μ mol/L; (4.12 ±0.57),(5.47 ±0.85) mg/L vs.(2.73 ±0.54) mg/L],ABI was lower than that in control group(0.92 ± 0.18,0.81 ± 0.16 vs.1.02 ± 0.23),and the difference was statistically significant (P < 0.05);Hcy,CRP in PVD group were obviously higher than those in DM group,ABI was lower than that in DM group,and the differences were statistically significant (P< 0.05).Spearman analysis showed that Hcy was negatively correlated with ABI (r =-0.635,P < 0.05),CRP was negatively correlated with ABI (r =-0.529,P < 0.05),and Hcy was positively correlated with CRP (r =0.528,P < 0.05).The partial correlation analysis results showed that Hcy and ABI still showed a negative correlation (r =-0.315,P < 0.05),CRP and ABI still showed a negative correlation (r =-0.243,P < 0.05).Conclusion Hcy and CRP participating in the occurrence and development of type 2 diabetes PVD,can be used as a predictor of type 2 diabetes PVD.
4.Study on quality control before application and after maintenance of the infantincubator
Qiyun YAN ; Yuwen ZHOU ; Xin PENG
China Medical Equipment 2017;14(5):126-128
Objective:To implement quality control in whole life cycle for infant incubator, and ensure it working in safe and efficient situation.Methods:The parameter detection cases of infant incubator was analyzed and researched according to relative standards and requirements of technique for medical equipment.Results:The detection data and frequent failures of clinical cases revealed that there were parameter deviation existing in infant incubators. These deviations should be adjusted in time according to relative standards and requirements of technique for medical equipment.Conclusion:The quality control of whole life cycle for infant incubator is the necessary condition for ensuring medical quality and safety of patients. To pay attention to the quality control before and after application and after maintenance of the infant medical incubator, in order to ensure the medical equipment can achieve to meet the performance requirement of treatment and provide safe and efficient quality service for patients.
5.Differentiation of neural stem cells from the anterior subventricular zone into dopaminergic neurons induced by astrocyte-conditioned medium in vitro
Zhicheng XIN ; Zheng ZHOU ; Jiwen YAN
Chinese Journal of Tissue Engineering Research 2007;0(06):-
BACKGROUND: Effective proliferation in vitro of neural stem cells and directional induction and differentiation of dopaminergic neurons play a key role in neural stem cell transplantation for treatment of degeneration of the nervous system and repair of injured nervous system. OBJECTIVE: To explore the differentiation of neural stem cells from anterior subventricular zone into dopaminergic neurons in astrocyte-conditioned medium. DESIGN, TIME AND SETTING: The in vitro controlled cytology experiment was performed at the Experimental Center of Xinqiao Hospital of Third Military Medical University of Chinese PLA from December 2006 to August 2007. MATERIALS: A total of 15 neonatal KM mice aged 2 days and 40 pregnant 16 days Wistar rats were provided by the Experiment Animal Center of Third Military Medical University of Chinese PLA. METHODS: Astrocytcs of KM mice were isolated and purified by a standard shaking method and differential adhesion. Astrocyte-conditioned medium was collected when astrocytcs at passage 3 were cultured for 5 days maintained at –20 ℃ for use. Neural stem cells from the anterior subventricular zone of Wistar rats were isolated and cultured in serum-free DMEM/F12, supplemented with B27 and basic fibroblast growth factor for primary culture. Neural stem cells at passage 3 were collected and incubated at 0.5?108/L. Cells in the control group were incubated in the DMEM/F12 medium containing fetal bovine serum of 0.1 volume fraction. Cells in the experimental group were incubated in the astrocyte-conditioned medium. MAIN OUTCOME MEASURES: Neural stem cells in the anterior subventricular zone were determined by the immunocytochemical technique. Positive rate of the neural stem cell differentiation into dopaminergic neurons in the anterior subventricular zone was detected by the flow cytometry. RESULTS: Cells in neurospheres expressed nestin and differentiated into neuro-specific enolase and glial fibrillary acidic protein. Tyroxine hydroxylase positive cells were found in the experimental group, showing round or ellipse. Tyroxine hydroxylase was seen in cytoplasm and cluster-shape processes. Tyroxine hydroxylase positive cells in control group were different from that in the experimental group in amount and appearance. Positive cell rate of tyroxine hydroxylase was significantly higher in the experimental group than in the control group (t =35.296, P
6.Interaction among peroxisome proliferators-activated receptor alpha, cytochrome P450 oxysterol 7α-hydroxylase and estrogen receptor and its association with intrahepatic cholestasis in pregnant rats
Qingyun SHI ; Yugeng LIN ; Xin ZHOU ; Yingqi LIN ; Shi YAN
Chinese Journal of Obstetrics and Gynecology 2010;45(6):440-444
Objective To investigate the relationship between interaction of peroxisome proliferators-activated receptor alpha (PPARα), cytochrome P450 oxysterol 7α-hydroxylase (CYP7B1) and estrogen receptor (ER) and intrahepatic cholestasis in pregnant rats. Methods Eighty clean SD pregnant rats were selected and divided into four groups randomly with 20 in each. Since the 13th day of pregnancy,rats in the control group was injected subcutaneously with refined vegetable oil 2.0 ml · kg-1 · d -1 , those in the low-dose, moderate-dose and high-dose groups received 17-α-ethynylestradiol (EE) 1.0 mg · kg-1 · d-1,1.25 mg · kg-1 · d-1 and 1.5 mg · kg-1 · d-1, respectively. All rats were sacrificed at the 21at day of pregnancy and maternal hepatic tissues were collected. The serum levels of alanine aminotransferase(ALT), aspartate transaminase (AST), total bile acid (TBA) and bilirubin (BIL) were determined by enzyme linked immunosorbent assay (ELISA). The mRNA expressions of PPARα, CYP7B1, Erα and Erβ in maternal rat livers were examined by real-time PCR. Results (1) Biochemical indicators: the serum levels of ALT,AST, TBA and BIL were significantly lower in the control group than in the rest 3 groups,respectively [ control group: (41.1 ± 2.8 ) U/L, (44.4 ± 3.6) U/L, (26.4 ± 5.6 ) μmol/L and( 2.8 ± 0.2)U/L;low-dose group: (48.2 ±3.4) U/L,(47.9 ±3.7) U/L,(36.4 ±4.2) μmol/L and (4.2 ±0.2) U/L;moderate-dose group: (70.4 ± 5.3 ) U/L, (68.4 ± 5.6) U/L, (64.3 ± 3.8 ) μmol/L and ( 6.2 ± 1.2)U/L; high-dose group: (72.4 ±7.6) U/L, (70.2 ±3.8) U/L, (72.4 ±7.8) μmol/L and (8.2 ±2.2)U/L, P<0.05], and those in the moderate or high-dose groups were higher than in the low-dose group (P<0.05). (2) mRNA expression of Erα and Erβ: the mRNA expression of Erα in pregnant rat livers increased in a dose-dependent manner, which were all significantly higher than that in the control group,respectively ( low-dose group: 0.76 ± 0.02 ); moderate -dose group: ( 0.99 ± 0.04; high-dose group:1.21 ±0.01 ;control group:0.65 ±0.01, P <0.05), but no difference was found among the 4 groups in the mRNA expression of Erβ ( P > 0.05 ). (3) mRNA expression of CYP7B1 and PPARα: the mRNA expression of CYP7B1 in pregnant rat livers increased from the low-dose group to the high-dose group, and were all higher than that of the control group ( low-dose group: 0.93 ± 0.01; moderate-dose group: 0.99 ±0.06; high-dose group: 1.22 ± 0.04; control group: 0.75 ± 0.02, P < 0.05 ). However, the mRNA expression of PPARα decreased from the low-dose group to the high-dose group, and were all lower than that of the control group (low-dose group: 0.83 ± 0.05; moderate-dose group: 0.71 ± 0.02; high-dose group:0.64 ± 0.03; control group: 1.35 ± 0. 05; P < 0.05 ) . Conclusions The down regulated mRNA expression of PPARα, caused by higher dose of estrogen, may increase the expression of CYP7B1 due to the ineffectiveness of the inhibition of PPARα on CYP7B1, which may further stimulate the Erα activity and then induce intrahepatic cholestasis. Abnormal expression of PPARα, CYP7B1 and ER may play a role in the pathogenesis of estrogen-induced intrahepatic cholestasis.
7.Current situation,outcome analysis and trends of the health reform of Changchun city
Guohua QI ; Qinglong LI ; Yan HUANG ; Fen ZHOU ; Xin LI
Chinese Journal of Hospital Administration 2015;31(6):414-417
Furthering of the health reform calls for better health care system,greater public financing,and deepened reform of public hospitals.Introduced in this paper are the actions taken and outcomes of the health reform in Changchun city,including the essential medicines system to minimize drug prices,improvement of the primary healthcare system to minimize people's financial burden,greater public spending to enhance public welfare nature of medical institutions,enhanced competencies of primary medical institutions to introduce rational classification of patient flow,and enhanced equity of public services to promote people's health.On such basis,further reform proposals were made for Changchun's health reform.
8.Effect of Fosinopril on oxidative stress of pancreatic tissue in diabetic rats
Jiqiang YAN ; Dongfang LIU ; Houdi ZHOU ; Xin XU
Journal of Third Military Medical University 1984;0(02):-
Objective To study the oxidative stress in diabetic rat pancreas,and the changes of islet function after intervention of Fosinopril.Methods Wistar rats were randomly divided into control group,diabetes group and Fosinopril treated group.The diabetes was induced by feeding the rats from diabetes group and Fosinopril group with high fat diet for 3 weeks and then once intraperitoneal injection of STZ(35 mg/kg),while additionally Fosinopril(5 mg/kg) was given to the diabetic rats in Fosinopril treated group.Blood glucose,body weight,nitrotyrosine levels in pancreatic tissues were detected and the histopathologic changes of pancreatic tissues were observed.Results Nitrotyrosine content was significantly higher in diabetes group than that in control group(P
9.Determination of Nitroglycerin Ointment by HPLC
Guixiang ZHONG ; Jia YAN ; Xin ZHOU ; Aiwen HUANG ; Hongtao SONG
China Pharmacist 2017;20(1):182-183,184
Objective:To optimize and improve the content determination method for nitroglycerin ointment. Methods:An HPLC method was used,the column was Hypersil ODS(150 mm × 4. 6 mm,5 μm),the mobile phase was acetonitrile ∶water(50 ∶50),the detection wavelength was set at 220 nm,the flow rate was 1 ml·min-1 ,the column temperature was 30℃,and the injection volume was 20 μl. Results: The results showed a good linear relationship within the concentration range of 0. 020 3-0. 203 3 mg · ml-1 ( r =0. 999 9),and the average recovery was 99. 51%(RSD=1. 06%,n=9). Conclusion: The method is rapid,accurate and reproduci-ble,and can be used to determine the content of nitroglycerin in nitroglycerin ointment.
10.Evaluation of research quality for medical graduates
Danfei XU ; Xing CHANG ; Yuqing PEI ; Yan LIU ; Xin ZHOU
Chinese Journal of Medical Education Research 2017;16(8):777-779
Objective To investigate the research quality of present medical graduates and propose suggestions for improvement.Method A self-designed questionnaire entitled Investigating Questionnaire for Research Quality Analysis of Medical Graduates in Peking Union Medical College Hospital was distributed to the clinical and academic medical graduates in the hospital from July to August 2015.Among 276 collected questionnaires,270 were validated as effective.SPSS 18.0 software was used to statistically describe the result and to perform t test on different subgroups.Results The research quality average of the surveyed hospital was 10.28.Results revealed that in regard of the three aspects of research quality,research consciousness ranked first,followed by the research ability,while scientific spirit was the weakest.Academic medical graduates showed significantly higher scores than clinical graduates in terms of total research quality and every single aspect (P values less than 0.05).Conclusions To better cultivate the academic leaders in medical research,it is necessary to strengthen the research training for the graduates,practice their scientific thinking especially the clinical graduates,reinforce tutors' guidance,and promote communication and collaboration.