Objective To investigate The effect of TAM(2.5 uM)in the chemotherapy of HCC and its mechanism. Methods An adriamycin-resistant human HCC cell subline(Hep-3B/ADM)was established,through exposure to gradually increased concentration of ADM.Drug sensitivity was measured by MTT.Modulation of ADM-induced apop- tosis of Hep-3B/ADM cells was assessed by Flow cytometry(FCM).PKC activity was determined by applying compet- itive protein binding method.Results TAM(2.5uM )decreased the IC50 of ADM from 1.64?g/mL to 0.23?g/mL in the Hep-3B/ADM cells.TAM exhibited a 2-fold potentiation on apoptosis caused by ADM in Hep-3B/ADM cells.With TAM,PKC activity was lower than that in control groups from 30 seconds to 24 hours.But this effect of TAM could be reversed by the co-treatment of the cells with TPA.Conclusion TAM(2.5 uM)significantly en- hanced ADM-induced cytotoxicity.The mechanism is that TAM potentiates the apoptosis induced by ADM through pro- tein kinase C inhibition.

Objective To explore the application effect of quality control for medical records of clinical pathway .Methods The 127 clinical pathway patients with acute ST segment elevation myocardial infarction were randomly divided into two groups .69 patients with clinical pathway quality control were taken as test group , while 58 patients without clinical pathway quality control were taken as control group.The hospitalization duration, total hospitalization expenses and various events expenses were compared between these two groups.Results The average hospitalization duration in test group was (10.32 ±2.03) days, while that in control group was (12.10 ±2.96) days, with statistically significant differences between the groups (p<0.05).The average hospitalization ex-pense in the test group was (52,349.94 ±16,944.22) yuan, while that in control group was (59,522.81 ±22,783.01) yuan, with statistically significant differences between the groups (p<0.05).Among various events of hospitalization expense, the medicine cost and nursing fee in the test group were lower than those in control group, with statistically significant differences (p<0.05), and no statistically significant differences were found in the other items (p>0.05).Conclusion The quality control for medical records of clinical pathway can effectively control the hospitalization duration and hospitalization expense , standardizing the implementation of clinical pathway.

ve To assess the cytogenetic toxicological safety of fish raised in water containing effluent. Methods The mutagenecity of organic extracts of uncooked meat specimens of fish raised in fishpond water mixed with sewage water taken from South and North Sewer Drainage Canals of Tianjin was detected by mice bone marrow micronucleus test. Results The organic extracts of uncooked fish meat specimens showed positive result for all of the 3 observed groups of fish raised in water of fish ponds with sewage, and negative result for fish raised in clean fishpond water in micronucleus test. Conclusion The results indicated that some kinds of organic mutagenic compounds ex-isted in the uncooked meat of fish raised in fishpond water containing effluent, which might have some potentially hazardous effects on human health through the food chains.

Objective To study the mutagenicity of fish-raising water containing effluent. Methods The niuta-genieities of water samples from waste water canal, the fish-raising water(fish-pond water) treated by presedimenta-tion in oxidation tank were detected using micronucleus test of vicia faba root tip cells. The micronucleus test of peripheral red blood cells was carried out among fish raised in water containing effluent. The water samples and fish collected from reservoir as controls. Results The micronucleus test of vicia faba root tip cells showed that the micronucleus rates in treated fish-pond water and waste water were significantly higher than those of negative control group (distilled water) (P 0.05) . The frequencies of micronucleus in peripheral red blood cells of fish raised in treated fish-pond water were significantly higher than those of fish raised in reservior water (P

Objective To probe the diagnostic value of MRI in synovial hemangioma of the knee.Methods Seven patients(2 male,5 female,age ranged from 8 to 58 years with mean age 25.1 years) selected from 2025 cases with MRI examination of knee,which were proved to be intra-articular hemangiomas histologically or clinically.All of the patients had plain film before MRI examination.MR findings of synovial hemangioma of knee were analysed ultrasound.Results Four hemangiomas occurred in infrapatellar bursa,others 3 cases in both infrapatellar and suprapatellar bursa were involved.MR signal intensity of hemangiomas appeared three types:(1)nodular hypointensity on both T_1WI and T_2WI in 2 cases;(2)iso-hypointensity on T_1WI and hyper-hypointensity on T_2WI in 2 cases;(3)hypointensity mixed with isontensity on T_1WI and nodular of linear mixedintensity on T_2WI in 3 cases.The difference of synovial hemangioma of knee in histologic elements,the MR findings were different.Conclusion MRI features of synovial hemangioma are correlated with pathology histologic elements.

OBJECTIVE:To compare between the HPLC method and gravimetric analysis in the determination of the content of sodium new Houttuyfonte injection.METHODS:The content of sodium new Houttuyfonte injection was determined by HPLC and gravimetric analysis respectively.HPLC condition: Zorba extent-C18 column(250 mm?4.6 mm,5 ?m) detected by UV(286 nm),and the mobile phase consisted of methanol-water-tetrabutyl ammonium hydroxide(70:30:0.3).The procedure of gravimetric analysis was in line with state drug standard.RESULTS: The determination results were notably different between the two methods.CONCLUSION:Gravimetric analysis showed no specificity in the determination of sodium new Houttuyfonte injection.

Objective To study the morphological changes, human bitemarks were made on the living and dead canine skin. Method The changing patterns of the human bitemarks were recorded with morphological parameters measured. The relationships among the patterns of the bitemark, tooth area, time, ocdusal force, tooth width and thickness were analyzed by multiple progressive regression. Results The multiple progressive regression equations of the morphological changes of human bitemark were established: S=-6.96-1.68?10 2T-0.11F +2.21W+3.75H(Live dog test,T

Objective Digital analysis of human bitemarks by blind method was assessed for its accura-cy. Method Digital analysis was used in double-blind method for identification of human bitemarks with control samples from 8 suspects. Photoshop 5.5 generates an overlay from bitemarks scanned and various coefficients were submitted for analysis by AutoCAD R14 engineering software to compare bitemarks with denture of suspects. Results Digital analysis shows that bitemarks tally with cast of denture from "suspects" in every coefficient. Conclusion Digital analysis is feasible for identification of experimental bitemarks and promises sound prospects in future forensic practice.

Objective To investigate how to prevent deep-vein thrombosis(DVT) after total knee replace-ment(TKR).Methods Deep vein thrombosis in 87 patients after TKR from 2004 to 2007 in the Third Hospital Affiliated to Suzhou University was retrospectively analyzed.Results Amony the 87 patients,13 were found having deep vein thrombosis,5 of 38 using low-molecular-weight beparin after TKR having deep vein thrombosis,6 of 35 cases using aspirin after TKR having deep vein thrombosis,5 of the 10 eases using mechanical preventive measures having deep vein thrombosis.The difference between low-molecular-weight heparin and aspirin group was not statistically significant.Conclusion Using low-molecular-weight heparin before TKR can prevent the occurrence of deep-vein thrombosis.Using epidural anesthesia,and a low-molee-ular-weight heparin drugs or aspirin after TKR can better prevent the formation of deep-vein thrombosis.

Objective To investigate the mechanism of acute patella bone cartilage fracture,and further study its diagnosis and treatment of clinical significance.Methods Collecting 21 cases of the acute patella bone cartilage fracture from July 2005 to December 2007.Eleven patients were male and ten were female,with the mean age of 31.6 (16～46) years.All the patients were treated using the arthroseopic microfracture technique after MR examination.Results All patients were followed up 6 to 18 months,did not show patellofemoral joint pain. Evaluated by Tagner rating,excellent outcomes were found in 17 cases,good in 4 cases,satisfactory results in 100% cases.Conclusion The arthroscopic microfracture technique in treatment of the patella cartilage fracture method is simple,easy to operate,can significantly improve knee function and pain,as well as the quality of life.