1.CT Diagnosis of Lumbar Posterior Marginal Intraosseous Cartilaginous Nodes
Journal of Practical Radiology 2010;26(4):547-548,570
Objective To discuss the CT diagnosis and differential diagnosis of lumbar posterior marginal intraosseous cartilaginous nodes(LPMN)and the possible pathogenesis.Methods CT manifestations of LPMN in 29 cases hospitalized in the last three years were analysed retrospectively.Results The mainly CT features of LPMN included:(1)Osseous defect with sclerotic margin in the posterior-superior or posterior-inferior margins of centrum;(2)Behind the defect area,bone fragments protrude into the spinal canal,totally dislocated or partially joined with centrum;(3)The dural sac and nerve root compressed accompanied by lumbar disc herniation and spinal canal stenosis.Conclusion LPMN can be definitively diagnosed by CT scan,which provides reliable basis for the treatment project.
4.The study of the washing methods for the piping instruments of laparoscope for prevention form biological membrane
Longjian GUAN ; Yan CHEN ; Xiaochun WU
Chongqing Medicine 2013;(33):4024-4025
Objective To study the washing methods for the piping instruments of laparoscope and prevention form biological membrane .Methods 300 pieces of the piping instruments of laparoscope select from the center of sterilization supply were random classified into 3 groups(100 pieces in each group) according to washing methods :washing by hand(group A) ,full-automation wash-ing machine(group B) and Tri-band pressurized ultrasonic washing machine(group C) .Comparing the cleanliness and ATP detec-tion fluorescence value on different methods .Results Cleanliness of group A was 85% ,ATP fluorescence detection rate was 27% ;Cleanliness of group B was 82% ,ATP fluorescence detection positive rate was 31% ;Cleanliness of group C was 99% ,ATP fluores-cence detection positive rate was 5% .The test data of group C was significantly compare with the data of group A and B . Conclusion The washing method of using tri-band pressurized ultrasonic washing machine can effectively improve laparoscopic tube cavity equipment cleaning effect and prevent form biological membrane .
5.Role and bioinformatics analysis of UCP2 in cervical cancer
Jing JIANG ; Yan CHEN ; Hong WU
The Journal of Practical Medicine 2017;33(1):91-94
Objective To investigate the effect of UCP2 on cell proliferation, apoptosis, metastasis in cervical squamous carcinoma. Methods Plasmid?mediated downregulation of UCP2 was obtained in cervical cancer cell lines. MTT, flow cytometry and transwell chamber assay were conducted to detect the ability of proliferation, apoptosis and metastasis. Characteristics of UCP2 protein was analyzed by bioinformatics methods. Results (1)UCP2 was verified to be downregulated by qRT?PCR and Western blotting assay.(2)MTT, apoptosis assay and transwell chamber assay showed that the proliferation of SiHa cell was significantly inhibited, and the apoptosis rate was significantly increased, and metastasis was markedly deduced (P < 0.05). (3) Bioinformatic analysis showed that UCP2 was located in mitochondria with several phosphorylation sites, and UCP2 interacted with other proteins to produce biological effects. Conclusion UCP2 may play an important role in the occurrence and development of cervical cancer, and it is expected to be a new target for the early diagnosis and treatment of cervical cancer.
6.Effect of selective cyclooxygenase-2 inhibitor NS-398 on cell apoptosis in K562 cells and its underlying mechanism
Chun ZHANG ; Qing WU ; Yan CHEN
Chinese Pharmacological Bulletin 2003;0(07):-
Aim To investigate the effect of selective cyclooxygenase-2 inhibitor, NS-398, on cell apoptosis in leukemia cell line K562 cells and its underlying mechanism. Methods Apoptotic cell percentage was examined by flow cytometry(FCM). The protein expression of Bcl-2 and Caspase-3 was detected by Western blot, and the activity of Caspase-3 was checked by FCM. Results NS-398 induced the cell apoptosis in K562 cells in a dose-dependent manner. After NS-398 treatment, the expression of Bcl-2 protein was downregulated, whereas the expression of Caspase-3 protein was upregulated. Moreover,the activity of Caspase-3 was increased in a dose-dependent way after NS-398 treatment. Conclusion Selective cyclooxygenase-2 inhibitor,NS-398,significantly induced apoptosis in K562 cells. The underlying mechanism might be related to the downregulation of Bcl-2 and the activation of Caspase-3.
7.The clinical efficacy of treatment of diabetic nephropathy with Shuxuening injection combined with benazepril
Guangyu WU ; Sishi YAN ; Xuebo CHEN
Chinese Journal of Biochemical Pharmaceutics 2017;37(4):180-182
Objective To observe the clinical efficacy of treatment of diabetic nephropathy with Shuxuening injection combined with benazepril.Methods 60 cases from January 2014 to January 2015,were randomly divided into observation group and control group of 30 patients.the control group were given benazepril treatment,observation group were based on Shuxuening injection treatment,patients were followed up and record changes related indicators.Results The urinary β2-MG and hs-CRP were after treatment(0.38±0.08,6.29±1.40)mg/L,than the control group(0.48±0.10,7.74±1.36)mg/L,and the difference was significant sex(P<0.05); after treatment observation group 24H urine protein quantitative detection of(0.69±0.50)g/24h,than the control group(1.04±0.63)g/24h,and the difference was statistically significant(P<0.05); After the observation group patients,low-density lipoprotein,serum creatinine value were(359.2±50.3mg/L,2.40±0.63mmol/L,95.6±22.3mol/L),were better than the control group(379.8±48.3mg/L,3.44±0.76mmol/L,108.5±34.2mol/L),and the difference was statistically significant(P<0.05); observation group total effective rate was 90.0%,higher than 73.0 percent,and the difference was statistically significant(P<0.05).Conclusion Shuxuening injection combined with benazepril clinical treatment of diabetic nephropathy exact,no significant side effects,is worthy of further research and application.
8.Non operative and simple operative managements of calcaneal fractures
Wei WU ; Hesheng CHEN ; Yan LIN ;
Chinese Journal of Orthopaedic Trauma 2004;0(10):-
Objective To evaluate non operative and simple operative managements of the calcaneal fractures. Methods 97 patients with calcaneal fractures were treated by non operative management or percutaneous reduction with wires or screw fixation from April 1997 to December 2001 in our department. They were followed up for more than 2 years. 64 patients were treated with non operation, 27 were fixed with wires and 6 with screws. Results 23 patients had excellent results, 67 good, 5 fair and 2 poor. The total good and excellent rate was 92.7%. 2 patients experienced secondary arthrodesis 1 year after injury. Conclusion Although the calcaneal fractures can be treated satisfactorily with non operative management or percutaneous reduction with wires or screws fixation, further prospective and comparative studies should be done in clinic to determine whether the calcaneal fractures should be treated with open reduction and fixation or with simple operative management.
9.Ultrasound diagnosis of amnniotic bands syndrome-clinical analysis
Min QIAN ; Qingqing WU ; Yan CHEN ;
Chinese Journal of Perinatal Medicine 1998;0(01):-
Objective To improve the diagnosis of amniotic bands syndrome with ultrasound. Methods All pregnant women underwent ultrasonographic examination to observe the intra amniotic band like structures. Results Among 8771 women undergone ultrasound examination, 60 were found with intra amniotic band structure. Seven cases were diagnosed as amniotic bands syndrome and 53 intra amniotic band like structures included extra amniotic pregnancy(7), amniotic sheets(4), incomplete uterine septum(24), fetal nuchal cystic hygroma(2), fetal nuchal and trunk edema(11) and disclosed extra embryo sac(5). Conclusions Not all the intra amniotic band like structures is amniotic band. But any abnormal structures detected in early stage should be closely followed up.
10.Microsurgical treatment of the thenal wounds
Zhi CHEN ; Chunhui WU ; Zhijian YAN
Chinese Journal of Microsurgery 2000;0(04):-
Objective To explore the methods in repairing the thenar wounds by microsurgical technique Methods Since 1989, thenar wounds were restored with transfer of superficial or deep palmar arch, recombine of thenar muscle flap Patch of nerve and transplantation of flap on 21 cases Results The thumb and flap were survived The two point discrimination was 5 to 10 mm The thenar muscle streugth reached MRC 4 with satisfactory oppones fuction Conclusion The thenal wounds cna be obtained good effect being repaired by microsurgical technique