Objective To explore the effect of osteopontin(OPN) as marker in colon cancer tissue and analyze the relationship between osteopontin and recurrence.Methods ELISA method was used to determine the serum levels of osteopontin in 58 patients with colon cancer(observation group) and 22 cases of normal subjects(control group);semi-quantity RT-PCR was used to detect the expression of osteopontin in tissues.The expression level of OPN mRNA in colon cancer tissue and serum OPN level in 23 recurrence cases and 35 non-recurrence cases one year after operation were observed.Results The OPN mRNA expression in colon cancer tissue and the serum osteopontin level in patients in observation group were higher than those in control group(P

Background and purpose:To investigate the clinico- pathological characteristics, prognosis and diagnosis of primary renal sarcoma that is a rare, highly malignant tumor with poor prognosis and survival. Methods:9 cases of renal sarcoma in our hospital were reviewed and analyzed. Nephrectomy was the main treatment for the patients. All the specimens were stained with histochemicial and immunohistochemical method, correlated literatures were also reviewed.Results:The distributions of pathology were malignant fibrous histiocytoma leiomyosarcoma, liposarcoma, myxosarcoma synoviosarcoma. Immunohistiochemical test was essential for the diagnosis of renal sarcoma.Conclusions:Pain and palpable mass were the common symptoms. Early radical surgery remains the optimal treatment, the role of chemotherapy and radiotherapy in the treatment modalities of the disease is still controversial.

Background and purpose:Fatty acid synthase(FAS)is a new tumor marker,the high expression of which has been reported to be associated with poor prognosis of cancer patients.This study investigated the expression of FAS in transitional cell carcinoma(TCC) of bladder and its clinical significance.Methods:The expressions of FAS were detected by streptavidin-peroxidase immunohistochemical method in the paraffin embedded sections of 65 TCC patients and the clinical data had been reviewed.15 normal bladder tissues were used as control group.Results:The positive rate of the expression of FAS in TCC was 58.5%(38/65),while only 2 of 15 cases in control group expressed FAS protein.The difference was statistically significant,the expression of FAS was related to the histological grades and recurrence but not different pathological stages.Survival analysis revealed that the 5-yr survival rate was lower in FAS positive patients than in negative patients(18% vs.44%,P

Objective To analyze the significance of global end-diastolic volume index (GEDVI) in acute kidney injury (AKI) after septic shock.Methods A retrospective analysis of 61 patients was performed.The patients were diagnosed of septic shock in emergency ward of Shenyang Military Hospital from 2012 March to 2013 May and were monitored by pulse indicator continuous cardiac output (PiCCO).The patients were divided into two groups:low GEDVI group (GEDVI ＜ 700 ml/m2,29 cases) and high GEDVI group (GEDVI≥700 ml/m2,32 cases) by evaluating GEDVI of 24 hour after PiCCO.Several physiologic and biochemical indexes were recorded,including the hemodynamic parameters at the beginning and the 24 h of PiCCO monitoring,Scr,BUN,lactic acid,incidence and mortality of AKI,baseline glomerular filtration rate,baseline Scr,APACHE Ⅱ scores,mortality during the period of emergency ward or within 28 d after the diagnosis.Results A total of 26 cases in high GEDVI group (81.3％) were attacked with AKI,while 16 cases in low GEDVI group (55.2％) were attacked with AKI,the incidence of AKI in high GEDVI group was significantly higher than that in the low GEDVI group.A COX regression analysis of mortality was performed between the patients staying at emergency ward and during 28 d after diagnosis.The results indicated that AKI and GEDVI had no relation with patients' death.Therefore,AKI and GEDVI could not be considered as the risk factors for the prognosis.Conclusions High GEDVI can significantly increase the incidence of AKI after septic shock,therefore high GEDVI should be avoided as much as possible in the course of clinical treatment.

Objective To investigate the changing patterns of Staphylococcus aureus (SA) and its resistance from a paediatric clinic in Beijing Children's Hospital Affiliated to Capital University of Medical Sciences.Methods SA isolated from hospitalized patients in Beijing Children's Hospital Affiliated to Capital University of Medical Sciences from Jan.2003 to Dec.2012 were analyzed.Antimierobial susceptibility was determined by disk diffusion method and Phoenix 100 microbiological system.Results were analyzed according to the 2012 guidelines of Clinical and Laboratory Standard Institute.WHONET 5.6 software was used to analyze the data.Results The clinical isolates of SA from inpatient were 2 843.The positive rates of SA were 10.0％,8.7％,9.0％,11.6％,12.7％,11.7％,10.5％,10.8％,11.9％ and 11.7％ form 2003 to 2012,respectively.The positive rate of Methicillin-resistant Staphylococcus aureus (MRSA) from inpatients was 10.9％.The positive rates of MRSA were 3.1％,5.4％,6.4％,4.1％,6.0％,10.6％,11.2％,12.7％,14.1％ aid 20.1％ form 2003 to 2012,respectively.The resistant rate of Methicillin-susceptible Staphylococcus aueus and MRSA to Penicillin were 91.4％ and 100.0％,Oxacillin were 0 and 100.0％,Cefuroxime were 0.5％ and 87.7％,Ceftriaxone were 0.1％ and 90.3％,Amoxicillin-clavulanic acid were 0.9％ and 86.7％,Erythromycin were 72.4％ and 92.2％,Clindamycin were 50.6％ and 80.3％,Ciprofloxacin were 2.2％ and 30.1％,Trimethoprim-sulfamthhoxazol were 12.2％ and 13.9％.Vancomycin-intermediate and Vancormycin-resistant Staphylococcus aureus were not found.Conclusions The prevalence of MRSA is increasing in paediatric inpatients.And MRSA isolates exhibit multidrug resistance.The sequential surveillance about SA is very important for guiding rational antimicrobial therapy and effective control of infections in paediatric patients.

Objective To observe the effect of electroacupuncture(EA) on the expression of glucose transporter-1(GLUT-1) of hippocampal microvascular endothelial cells in focal cerebral ischemia(CI) rats.Methods Sixty male Wistar rats were randomly divided into sham-operation,model and electroacupuncture(EA) groups.CI model was established by middle cerebral artery occlusion with a piece of thread embolus.EA(4 Hz/20 Hz,1 mA) was applied to bilateral "Neiguan"(PC 6) for 30 min,once daily for 15 days.GLUT-1 expression in the hippocampus was detected with immunohistochemistry and Western blot,respectively.Results After the treatment,compared with the sham-operation group,the expression of GLUT-1 in microvascular endothelial cells of the hippocampus in model group shown by both histochemical and Western blot techniques decreased apparently(P

Objective:To investigate the high flux hemodialysis (HFD) on the application effect of severe renal failure patients the level of inflammatory factors, blood lipid and protein. Methods:In 2012 January to 2013 December was performed in 80 patients with severe renal failure in the treatment of MHD, were randomly divided into two groups, study group 40 cases by high-throughput MHD treatment, 40 cases in the control group using conventional MHD treatment. Results: There was no significant difference in two groups before treatment of various inflammatory factors difference (P>0.05); after the treatment, two groups of patients with TNF- α, IL-6 and hs-CRP levels were significantly lower(t=14.138, t=5.891, t=11.093;P<0.01), and lower levels of TNF-α, IL-6 and hs-CRP levels than the control group after treatment, the study group, with statistical significance correlation between data comparison of difference (t=17.913, P<0.05). Comparison of the plasma protein levels had no significant difference between the two groups before treatment (P>0.05);after the treatment, patients in study group were TP, ALB andβ2-M compared with those before treatment were significantly improved, ALB of control group had obvious improvement than before treatment (P<0.05); improve study group for plasma proteins relevant indexes are the control group was significant (t=2.515, t=59.923, t=3.474, P<0.01). No significant differences between the level of each lipid index (P>0.05);after the treatment, patients in study group TC and TG compared with those before treatment were significantly improved after treatment, and compared with the control group index improved significantly (t=2.963, t=6.914; P<0.01). Conclusion:High throughput MHD therapy based on conventional MHD filtration of small molecular toxin traits on patients with severe renal failure, increase the filtration range, clear promote inflammatory molecules, plasma proteins, blood lipid molecules, such as in large molecules, so as to achieve a better therapeutic effect.

0.05;q=8.055,P0.05;q=9.163,P

OBJECTIVE To explore the canonical prevention and management method to improve the control quality safety of hospital infection in operating room,and the medical care quality.METHODS The key points included infection institution,nosocomial control,operating room management,effective infection disinfection,isolation and other measures.RESULTS According to canonical scientific management available to quality safety of infection prevention,it could prevent infection in operating room.CONCLUSIONS In order to prevent nosocomial infection the accomplishment of effective disinfection and isolation measures during contaminated operation in hospital operating room is an important guarantee to the medical care quality.

Objective To explore the relationship between plasma cholesterol ester transfer protein(CETP) and TaqIB gene polymorphism and type 2 diabetes mellitus(DM).Methods The genotypes of the first intron of CETP TaqIB gene in 102 patients with type 2 DM and 103 healthy controls were analyzed by polymerase chain reaction-restricted fragment length polymorphism(PCR-RFLP),and plasma CETP were measured by enzyme-linked immunosorbent assay(ELISA).The association of gene polymorphism with plasma lipids,lipoproteins and apolipoproteins was also analyzed.Results No significant differences in both of the alleles frequencies and genotypes in type 2 DM were noted in comparison with controls.There were no relationships between CETP TaqIB gene polymorphism and gender,family history,smoking and body mass index(BMI).No significant differences of plasma lipid levels were observed in healthy controls with different genotypes.However,significant differences in level of HDL-C or apoAI among different CETP TaqIB genotype groups were observed,and significant correlation between low high-density lipoproteinemia and B1 allele in type 2 DM was observed.Conclusion CETP TaqIB gene polymorphisms associated with lipid metabolism,which may be an important genetic factor for abnormal lipid metabolism in type 2 DM.