1.Research of resisted function of L-arginine on BP in stress rats.
Yan-Ru YIN ; Jin-Feng HAN ; Lin BAI
Chinese Journal of Applied Physiology 2002;18(2):172-185
Animals
;
Arginine
;
pharmacology
;
Blood Pressure
;
drug effects
;
Hypertension
;
etiology
;
metabolism
;
physiopathology
;
Male
;
Oxidative Stress
;
drug effects
;
Rats
;
Rats, Sprague-Dawley
2.Identification of a mutation in the arginine vasopressin receptor 2 gene in a Chinese pedigree with congenital nephrogenic diabetes insipidus
Xue-Ru CHEN ; Yan DONG ; Jun YIN ; Hui-Li XING ; Qing SU ;
Chinese Journal of Endocrinology and Metabolism 1985;0(02):-
Genomic DNA was extracted from the blood samples of 3 patients from 1 pedigree with congenital nephrogenie diabetes insipidus (NDI) and their 12 family members.The whole coding region of the arginine vasopressin receptor 2 (AVPR2) gene was amplified by PCR and then directly sequenced,A mutation of AVPR2 gene [g1236T→C (L292P)]was found in 3 patients.The patients' mothers were found to have both mutant and normal alleles.
3.Association between angiotensin converting enzyme gene polymorphism and cerebral infarction
Yu FU ; Yin-hua WANG ; Ru-ping XIE ; Dafang CHEN ; Yan ZHANG ; Dongsheng FAN ; Xiping XU
Chinese Journal of Rehabilitation Theory and Practice 2004;10(3):158-160
ObjectiveTo explore the association between angiotensin converting enzyme (ACE) gene insertion/deletion(I/D) polymorphism and cerebral infarction among Chinese people. MethodsThe ACE gene polymorphism was determined by PCR-RFLP in 242 patients with cerebral infarction and 283 controls. Multiple logistic regression was performed to explore the risk factors for cerebral infarction. ResultsAfter adjusting age, gender, alcohol drinking, smoking, education,history of diabetes mellitus and the primary hypertension, there was no significant association between ACE I/D polymorphism and cerebral infarction, either was hypertension and diabetes mellitus. The primary hypertension significanlly increased risk of cerebral infarction (OR=7.28,P =0.000). Both ACE ID/DD genotype and the primary hypertension showed a significant gene-environment interaction(r=1.62,OR=7.29), something as super multiplicative type 2 interaction. ConclusionAlthough ACE gene polymorphism is not risk factors of cerebral infarction, but ID/DD genotype had shown significant gene-environment interaction with primary hypertension in occurrence of cerebral infarcion.
4.Farnesoid X receptor in the study of fibrosis and its treatments.
Cong ZHAO ; Yu-ling CONG ; Yi-jun XU ; Yan-ru YIN
Chinese Medical Journal 2013;126(19):3775-3781
Animals
;
Cholestasis
;
complications
;
Fibrosis
;
etiology
;
Gallbladder
;
pathology
;
Humans
;
Kidney
;
pathology
;
Lipid Metabolism
;
Liver Cirrhosis
;
etiology
;
therapy
;
Myocardium
;
pathology
;
Receptors, Cytoplasmic and Nuclear
;
physiology
5.Progress on Correlation between the Expression of CDK5 and Brain Injury Time.
Shi-yu MA ; Ru-bo LI ; LUO YU-JIA ; Meng-yan LÜ ; Han-zhi WANG ; Zheng-yin WANG
Journal of Forensic Medicine 2016;32(1):58-60
Cyclin-dependent kinase 5 (CDK5) is a member of cyclin-dependent kinase family, which does not directly regulate cell cycle. Through phosphorylation of target protein, CDK5 plays an irreplaceable role in the development, reparation and degeneration of neurons. Brain injury refers to the organic injury of brain tissue caused by external force hit on the head. Owing to the stress and repair system activated by our body itself after injury, various proteins and enzymes of the brain tissues are changed quantitatively, which can be used as indicators for estimating the time of injury. This review summarizes the progress on the distribution, the activity mechanism and the physiological effects of CDK5 after brain injury and its corresponding potential served as a marker for brain injury determination.
Brain/physiopathology*
;
Brain Injuries/physiopathology*
;
Cyclin-Dependent Kinase 5/metabolism*
;
Nerve Tissue Proteins/metabolism*
;
Neurons
;
Neuroprotective Agents/pharmacology*
;
Phosphorylation/drug effects*
;
Time Factors
6.Bioactive compounds from Paecilomyces tenuipes regulating the function of the hypothalamo-hypophyseal system axis in chronic unpredictable stress rats.
Yan-Yan YIN ; Liang MING ; Li-Fang ZHENG ; Hong-Wei KAN ; Chun-Ru LI ; Wei-Ping LI
Chinese Medical Journal 2007;120(12):1088-1092
BACKGROUNDA bioactive compound from Paecilomyces tenuipes (BCPT) has an inhibitory effect on monoamine oxidase A (MAO-A) and monoamine oxidase B (MAO-B) in vitro and in vivo, which indicates BCPT may be a potential antidepressant. In this study we aimed to study the antidepressant effects of BCPT in the chronic unpredictable stress (CUS) model in rats and explore underlying mechanisms in the hypothalamic-pituitary-adrenal (HPA) axis.
METHODSThe antidepressant effects of BCPT were studied in the chronic unpredictable stress model in rats. Animals were housed isolated, except the control group. Rats were exposed daily to different random stressors from day 1 to 21. Awarding response was detected by calculating the 24-hour consumption of sucrose water. Cortisol (CORT) and adrenocorticotropic hormone (ATCH) contents in serum and arginine vasopressin (AVP) contents in the pituitary body were detected by radio immunoassays. Total RNA of hippocampus or hypothalamus was extracted and subjected to reverse transcription-polymerase chain reaction (RT-PCR) for the measurement of corticotrophin releasing hormone (CRH) mRNA or mineralocorticoid receptor (MR) mRNA and glucocorticoid receptor (GR) mRNA levels. Statistical analyses were performed using one way analysis of variance (ANOVA) followed by Student-Newman-Keuls (SNK) test.
RESULTSChronic unpredictable stress resulted in reduction of sensitivity to reward and abnormality in the HPA axis in the animal model. BCPT improved the reward reaction as measured by increasing sucrose consumption, remarkably reduced serum CORT and ACTH levels and the AVP content in the pituitary body in the CUS-treated rats, decreased the expression of CRH mRNA, enhanced the expression of hippocampus MR mRNA, GR mRNA and decreased the ratio of MR/GR.
CONCLUSIONSBCPT has potentially antidepressant-like activity and normalized the HPA axis hyperactivity in a CUS model of depression in rats. This may be an important mechanism of its antidepressant effect.
Animals ; Antidepressive Agents ; pharmacology ; Chronic Disease ; Corticotropin-Releasing Hormone ; genetics ; Hydrocortisone ; blood ; Hypothalamo-Hypophyseal System ; drug effects ; physiology ; Male ; Monoamine Oxidase Inhibitors ; pharmacology ; Paecilomyces ; chemistry ; RNA, Messenger ; analysis ; Rats ; Rats, Sprague-Dawley ; Receptors, Glucocorticoid ; genetics ; Receptors, Mineralocorticoid ; genetics ; Stress, Psychological ; physiopathology ; Sucrose ; administration & dosage
7.Incidence of pocket hematoma after electrophysiological device placement:dual antiplatelet therapy versus low-molecular-weight heparin regimen
Yan CHEN ; Yuntao LI ; Mingdong GAO ; Zechun ZENG ; Jinrong ZHANG ; Hongliang CONG ; Yin LIU ; Ru ZHAO ; Lefeng WANG ; Xincun YANG ; Kang MENG
Journal of Geriatric Cardiology 2014;(3):200-205
Background Given the increasing number of patients who require dual antiplatelet (DAP) therapy and electrophysiological device (EPD) placement, perioperative antiplatelet management is a current challenge. In this study, we investigated the incidence of pocket hema-toma formation after EPD placement in patients undergoing DAP therapy or an alternative low-molecular-weight heparin (LMWH) regimen. Methods This clinical observational study was performed from July 2010 to July 2012. In total, 171 patients were enrolled in the analysis after meeting the inclusion criteria. These patients were divided into two groups: 86 patients were treated with DAP therapy at the time of device implantation, and the DAP therapy was discontinued for 5 to 7 days and replaced with enoxaparin before device implantation in the other 85 patients. Adenosine phosphate (ADP)-mediated platelet aggregation and arachidonic acid-induced platelet aggregation were tested preoperatively. We compared the incidence of pocket hematoma between the two groups and the association of pocket hematoma develop-ment with ADP-mediated platelet aggregation and arachidonic acid-induced platelet aggregation.Results The incidence of pocket hema-toma in the patients who continued DAP was lower than that in the patients who replaced the dual antiplatelet regimen with LMWH (3.49%vs. 16.47%, respectively;X2 = 6.66,P < 0.01). Among the patients who continued DAP therapies, the rate of ADP-mediated platelet aggre-gation inhibition in patients with pocket hematomas was higher than that in patients without pocket hematomas. None of the patients under-going DAP or enoxaparin therapy developed pocket infection, thromboembolic events, or other serious complications. Multiple logistic re-gression analysis revealed that LMWH therapy was an independent risk factor for the development of pocket hematoma (RR = 0.054, 95%CI = 0.012-0.251). Furthermore, patients undergoing LMWH therapy were 5.1-fold more likely to develop pocket hematomas than were DAP-treated individuals.Conclusion Continuance of DAP therapy does not increase the risk of pocket hematoma formation after EPD placement.
8.Effect of semaphorin 4D on the proliferation, migration and angiogenic of human pancreatic carcinoma cells
jiang Ru JIA ; yan Li HOU ; chen Qing YIN ; fang Li LI ; wu Geng YANG
Journal of Xinxiang Medical College 2017;34(12):1063-1067
Objective To investigate the effects of semaphorin 4D(Sema4D) on the proliferation,migration and angiogenic of human pancreatic carcinoma cells.Methods Sema4D-siRNA was designed and synthesized and transfected into human pancreatic carcinoma cells.After 48 hours of transient infection,the changes of expression of Sema4D mRNA before and after transfection were detected by reverse transcription-polymeruse chain reaction method.And after 72 hours of transient infection,the changes of expression of Sema4D protein before and after transfection were detected by Western blot method.The changes of growth of the transfected cells were observed by methyl thiazolyl terazolium assay.Using transwell migration test and scratch repair test to detect the changes of migration ability of human pancreatic carcinoma cells after transfection.Using tubule formation assay to observe the effect of supernatant of pancreatic carcinoma cell cultures on angiogenesis after transfection.Results Compared with the negative control group and blank control group,the expression of Sema4D mRNA and Sema4D protein and the growth rate of pancreatic carcinoma cells decreased significantly (P < 0.05).In transwell migration test and scratch repair test,it was observed that Pancreatic cancer cells penetrating cell number and scratch repair rate were significantly lower than that in negative control group and blank control group (P < 0.05).Tubule formation assay showed that there were significant differences in angiogenesis numbers among siRNA transfection group(0.5 ± 0.02),negative control group(1.45 ± 0.60) and blank control group (1.37 ± 0.52) (P < 0.05).Conclusion Sema4D-siRNA can induce RNA interference in pancreatic carcinoma cells and down-regulate the expression of Sema4D gene,which can inhibit the proliferation of pancreatic carcinoma cells,significantly reduce the migration ability of pancreatic carcinoma ceils and inhibit angiogenesis.
10.Effects of using hand-held vibration tool on work-related musculoskeletal disorders in automobile factory workers
RAN Yan li LING Rui jie MEI Yong ZHENG Jian ru WU Jia bing ZHANG Kang kang LI Qin YIN Hong
China Occupational Medicine 2022;49(05):498-
Objective -
To analyze the effect of using vibration tools on the prevalence of work related musculoskeletal disorders
( ) Methods , -
WMSDs in automobile factory workers. By judgment sampling method front line workers with more than one year
of working experience in an automobile factory were selected as the research subjects. Musculoskeletal Disorders Questionnaire
was used for investigation. The workers were divided into the control group and the vibration tool group. The propensity score
∶ ,
matching method was used to balance the confounding factors of the two groups of workers by 1 1 and 568 people were
Results
included in each group. The prevalence of WMSDs was compared between the two groups after matching. After
, , , , , ,
matching the prevalence of WMSDs in the shoulder elbow hand/wrist upper back waist hip/buttock and knee of workers in
, ( P )
the vibration tool group was higher than that in the control group and the differences were statistically significant all <0.05 .,
The prevalence of WMSDs in different body parts of workers in the vibration tool group ranking from high to low was waist
, , , , , , , , , ,
neck shoulder hand/wrist upper back knee ankle/foot elbow and hip/buttock with the rate of 74.3% 61.3% 54.2%
, , , , , (P ) Conclusions
54.0% 50.9% 39.4% 35.2% 31.0% and 27.1% respectively <0.01 . The use of vibration tools can
, , , , ,
increase the risk of WMSDs in shoulder elbow hand/wrist upper back waist hip/buttock and knee of automobile factory
workers. Corresponding measures should be taken to reduce vibration intensity and reduce contact time to protect workers'