1.Inheritance of academic idea and experience about using traditional Chinese medicine from JIN Shi-yuan.
Yan JIN ; Rong LUO ; Lu-Qi HUANG
China Journal of Chinese Materia Medica 2014;39(16):3200-3202
Professor Jin Shi-yuan has been worked in traditional Chinese medicine (TCM) over 70 years. He made prominent contributions in identification, processing, dispensing of TCM and reasonable use proprietary Chinese medicine. In over 70 years, he has mastered herbal medicine and traditional Chinese Medicine. It is also professor JIN's academic characteristic. Professor JIN's practical experiences were summarized according to the current situation about clinical medication, change of species of Juhong and Chenpi has been different from species of medical history. The quality is lower than before. Medicinal parts of Danggui, Gancao, Huangqin and Wuyao has been changed. So the actions of these herbal medicines have been changed also. Fresh herbal Qianchangpu has disappeared but it should be used clinically. Medical history, change of species, change of medicinal part, and change of preparing process in professor JIN's academic idea were be summarized periodically. The result is hoped to be referred by administration, manufacture, medical treatment of TCM.
Chemistry, Pharmaceutical
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education
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history
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standards
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Drugs, Chinese Herbal
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chemistry
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standards
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Herbals as Topic
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history
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standards
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History, 20th Century
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History, 21st Century
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Humans
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Medicine, Chinese Traditional
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history
2.Expressions and clinical significances of Hepcidin, BMP6 and HJV in gastric cancer
Rong LI ; Ye LU ; Xu CHENG ; Min YAN ; Xiangtao PAN
Journal of International Oncology 2016;43(3):180-183
Objective To study the expressions of Hepcidin,bone morphogenetic protein 6 (BMP6) and hemojuvelin (HJV) in gastric cancer,and to explore their relationships with clinical pathological characteristics.Methods Hepcidin,BMP6 and HJV were detected by immunohistochemistry in 62 cases of gastric cancer patients,and the relationships among them and clinical pathological features were analyzed.Results Compared with normal gastric mucosa tissues,Hepcidin was highly expressed in gastric cancer tissues (13.3% vs.56.5%,x2 =8.99,P < 0.01),while the differences of the expression of BMP6 and HJV in the two groups were not statistically significant (60.0% vs.40.3 %,x2 =1.89,P > 0.05;93.3% vs.83.9%,x2 =0.88,P > 0.05).The expression of Hepcidin was related to T stage (x2 =6.98,P =0.02),but it was not related to age,sex,lymph node metastasis,distant metastasis and anemia.The expressions of BMP6 and HJV were not related to T stage,age,sex,lymph node metastasis,distant metastasis and anemia.Hepcidin was not related to the expressions of BMP6 and HJV (r=0.13,P>0.05;r=0.15,P>0.05).Conclusion Hepcidin is highly expressed in gastric cancer tissues,which is related to the T staging of gastric cancer,and can be used as an objective indicator of the biological behavior of gastric cancer.There were no differences in the expression of BMP6 and HJV between normal gastric mucosa and gastric cancer tissues.
3.Investigation on occupational manganese poisoning hazard in Guang Xi.
You-rong LU ; Yan-ning LI ; Hou-jian LI
Chinese Journal of Industrial Hygiene and Occupational Diseases 2009;27(10):616-617
Adult
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Aged
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Cohort Studies
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Dust
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analysis
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Female
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Humans
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Male
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Manganese
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analysis
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Manganese Poisoning
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diagnosis
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Middle Aged
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Occupational Exposure
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analysis
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Retrospective Studies
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Young Adult
4.Levels of interleukin-23 in asthmatic children before and after glucocorticosteroid treatment.
Yan-chun LI ; Ji-rong LU ; Shan-yu LI
Chinese Journal of Pediatrics 2007;45(3):218-219
Adolescent
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Anti-Asthmatic Agents
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therapeutic use
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Asthma
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blood
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drug therapy
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Child
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Child, Preschool
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Female
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Glucocorticoids
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therapeutic use
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Humans
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Infant
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Interleukin-23
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blood
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Male
5.Inhibition of invasive and chemotactic abilities of SKOV3 cells bv human epithelial growth receptor-2 small interfering RNA
Shu-Lan ZHANG ; Yan-Ming LU ; Li-Rong MENG ; Yan-Yan ZHAO ;
Chinese Journal of Obstetrics and Gynecology 2001;0(01):-
Objective To investigate the effects of RNA interference(RNAi)targeting human epithelial growth receptor-2(HER-2)gene on the invasive and chemotactic ability of SKOV3 cells. Methods Glyeeraldehyde-3-phosphate dehydrogenase(GAPDH)was used as the positive control. Lipofectamine 2000 mediated transient transfection was conducted to transmit the siRNA into SKOV3 cells. Three pairs of specifically targeted(HER-2 siRNA Ⅰ,HER-2 siRNA Ⅱ,HER-2 siRNA Ⅲ)sequence were selected in the coding region of HER-2 mRNA.Transfection of HER-2 siRNA was conducted with lipofeetamine 2000 in ovarian carcinoma cell line SKOV3.The HER-2 gene expression was assessed by real- time PCR and western blot assays.Changes of invasive and chemotaetie capacity of SKOV3 cells were measured by polycarbonates coated with or without matrigal.Results Western blot results showed that the expression of GAPDH protein was decreased in specifically transfected cells and with the increase of siRNA dose,the expression of GAPDH protein was decreased.GAPDH protein gray value in control group, different doses(0.5,1.0,1.5,2.0 ?g)GAPDH siRNA interference groups were 0.6855?0.0259,0.5698 ?0.0275,0.4542?0.0296,0.3341?0.0178 and 0.1816+0.0180,respectively.There was a significant difference in each group(F=198.126,P
6.Levels of main platelet thrombin receptors in older chronic haemodialysis patients.
Yan LI ; Lin SHEN ; Rui CHEN ; Fu-rong LU ; Jing LI ; Jian-guo LIU
Chinese Medical Journal 2010;123(17):2495-2496
Aged
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Aged, 80 and over
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Blood Platelets
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chemistry
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Humans
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Receptor, PAR-1
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blood
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Receptors, Thrombin
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blood
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Renal Dialysis
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Uremia
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blood
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therapy
7.Research advances in value of flow cytometric immunophenotyping in diagnosis and prognostic evaluation of myelodysplastic syndrome.
Journal of Experimental Hematology 2013;21(3):785-790
Myelodysplastic syndromes (MDS) are myeloid neoplasms characterized by dysplasia in one or more linages of cells and increased risk of development of acute myeloid leukemia (AML). Along with the deeply understanding of myelodysplastic syndrome, the diagnosis standards of this disease experienced a leap in essence: from a single standard of morphological test in FAB to multiple detecting means in WHO standard of 2008, flow cytometry has been proposed as an adjunctive diagnostic test in the 2007 Vienna standards and the 2008 WHO standards. Recently, A heterogeneous spectrum of immunophenotypic abnormalities have been reported in MDS, and some of which are of great significance to the diagnosis, classification, prognosis assessment, and treatment of the disease. In the year of 2003, a flow cytometric scoring system (FCSS) was built to evaluate the prognosis of MDS patients, which was able to qualify the phenotypic aberrancies in the myelomonocytic, erythroid, and megakaryocytic lineage. It filled the gap of the international prognostic scoring system (IPSS) and the WHO classification-based prognostic scoring system (WPSS), and was of great value to the clinical diagnosis and treatment of MDS. In this article, the value of MDS immunophenotyping in diagnosis and prognosis evaluation of MDS is reviewed in term of MDS immunophenotypic abnormalities and flow cytometric scoring system.
Flow Cytometry
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Humans
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Immunophenotyping
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Myelodysplastic Syndromes
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classification
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diagnosis
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immunology
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Prognosis
8.Cloning and prokaryotic expression analysis of squalene synthase 2 (SQS2) from Salvia miltiorrhiza f. alba.
Qi-xian RONG ; Dan JIANG ; Liang-ping ZHA ; Ye SHEN ; Yan ZHANG ; Lu-qi HUANG
China Journal of Chinese Materia Medica 2015;40(7):1259-1265
According to the designed specific primers of gene fragment based on the Salvia miltiorrhiza transcriptome data, a full-length cDNA sequence of SQS2 from S. miltiorrhiza f. alba was cloned by the method of reverse transcription polymerase chain reaction (RT-PCR). The SmSQS2 cDNA sequence was obtained, this sequence is named SmSQS2 and its GenBank registration number is KM244731. The full length of SmSQS2 cDNA was 1245 bp, encoding 414 amino acids including 5'UTR 115 bp and 3'UTR 237 bp. Sequence alignment and phylogenetic analysis demonstrated that SmSQS2 had relative close relationship to the SQS2 of S. miltiorrhiza. The induction of E. coli [pET28-SQS2] in different temperature, induction time, IPTG concentrations and density of inducing host bacterium (A600) were performed, Shaking the culture at 30 degrees C until the A600 is approximately 0.6 and add IPTG to final concentration of 0.2 mmol x L(-1), and then the optimal expression of SmSQS2 recombinant protein were accumulated after the induction time of 20 h. The research provided important base for the study of sterol and terpene biosynthesis of SQS2 in S. miltiorrhiza f. alba.
Cloning, Molecular
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Farnesyl-Diphosphate Farnesyltransferase
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chemistry
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genetics
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metabolism
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Models, Molecular
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Molecular Sequence Data
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Phylogeny
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Plant Proteins
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chemistry
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genetics
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metabolism
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Salvia miltiorrhiza
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chemistry
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classification
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enzymology
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genetics
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Sequence Alignment
9.Sleep structure and cognitive function in stoke combined with obstructive sleep apnea hypopnea syndrome
Qiaoli LU ; Rong XUE ; Lixia DONG ; Li REN ; Haiyan CAO ; Nan ZHANG ; Yan CHENG
Chinese Journal of Neurology 2012;45(6):400-403
Objective To explore characteristics of sleep structure and the correlation with cognitive function in cerebral infarction combined with obstructive sleep apnea hypopnea syndrome (CI-OSAHS).Methods The patients with CI-OSAHS and OSAHS in Department of Neurology and Breathing Sleep Monitoring Room of Tianjin Medical University General Hospital from December 2009 till March 2011 were collected All the patients completed polysomography(PSG).Sixty patients were selected and divided into 3 groups based on PSG.These 3 groups were combined group 20 persons (CI-OSAHS),OSAHS group 20persons (OSAHS) and control group 20 persons (without cerebral infarction obstructive sleep apnea hypopnea syndrome).All the patients completed image examinations ( CT and MRI ) evaluation of the cognitive function by Mini-Mental State Examination(MMSE) and Montreal Cognitive Assessment(MoCA).Results Sleep structure:the awake time,non-rapid eye movement sleep (NREM) 1,NREM 2 and NREM periods in combined group and OSAHS group were significantly longer,the NREM3 + 4 and rapid eye movement(REM) periods were shorter than the control group.The NREM and NREM 1 periods in combined group were longer,the NREM 3 +4 and REM periods were shorter than the OSAHS group.The correlation analysis of cognitive function and breathing disorders and low oxygen related index:there was negative correlation between the total scores of cognitive function (MMSE and MoCA)and apnea hyponea index,oxygen desaturation index (ODI) ( MMSE r =-0.450,-0.671,MoCA r =-0.486,- 0.494,all P <0.05) while,was positive correlation between them and noctumal average hypoxemia and minimum hypoxemia ( MMSE r =0.477,0.485,MoCA r =0.507,0.482,all P <0.05) in the OSAHS group.There was negative correlation between ODI,arousal index and the total scores of MoCA in the combined group (MoCA r=-0.463,0.480,both P<0.05),there was correlation between the total scores of MMSE and the other sleep parameters,but,there was no difference in statistics.The correlation analysis of cognitive function and sleep stages:There was positive correlation between the total scores of cognitive function ( MMSE and MoCA) and the NREM 3 + 4 periods ( r =0.521,0.474,both P < 0.05 ) while,there was negative correlation between the total scores of MMSE and the N REM 1 + 2 periods (r =-0.458,P < 0.05 )in the OSAHS group.There was positive correlation between the REM period and the total scores of MoCA (r =0.472,P < 0.05 ).There was correlation between the total scores of MMSE and the sleep structure,but,there was no difference in statistics in combined group.Conclusions Patients with OSAHS have obvious sleep structure disorder.The awake time and light sleep periods are significantly longer than the control group,while,the deep sleep and REM periods are significantly shorter than the control group.The NREM 1 of the patients with CI-OSAHS is longer than the patients with OSAHS.The higher the AHI,the lower the night blood oxygen,the more obvious cognitive dysfunction The longer the awake time,the longer the light sleep,the shorter the deep sleep and REM periods,the more serious cognitive dysfunction.The correlation between the cognitive impairment and low oxygen is more apparent than sleep structure.There is apparent correlation among the total scores of MoCA,the degree of hypoxia and sleep structure in the patients with CI-OSAHS.The total scores of MoCA are more sensitivity than MMSE in mild vascular cognitive impairment.
10.Twenty cases of cerebral venous sinus thrombosis presented as isolated intracranial hypertension
Yang LU ; Shilei CUI ; Rong YAN ; Houliang SUN ; Zhenchang WANG ; Xiaojun ZHANG
Chinese Journal of Neurology 2013;(2):112-116
Objective To investigate the clinical and image features of cerebral venous sinus thrombosis (CVST) presented as isolated intracranial hypertension.Methods The medical records of patients with diagnosis of CVST presented as isolated intracranial hypertension were reviewed.Clinical features and imaging data were retrospectively analyzed.Results Twenty cases of CVST were included,all these patients were clinically presented as isolated intracranial hypertension.The male to female ratio was 13:7,and the average age was (38.3 ± 11.7) years old.None of the patients was obesity.The visual acuity was lower than 0.1 in 42.5% (17/40)of the eyes.Possible risk factors relevant to CVST were found in 11 cases (55%),including head trauma for 4 cases,autoimmune disease for 2 cases,and other causes of single case including spontaneous abortion,phlebitis,otitis media postoperative,trigeminal nerve microvascular decompression surgery and obstructive sleep apnea syndrome.Image analysis showed that lateral sinus thrombosis was involved in 85% (17/20) of the patients,while superior sagittal sinus was involved in 35% (7/20),and 65% (13/20) of the patients were isolated lateral sinus thrombosis.Conclusions Young male predominance is found in CVST patients which presented as isolated intracranial hypertension but severe visual function loss.Risk factors such as head trauma are commonly found in these patients.Most of the patients are isolated lateral sinus thrombosis,with lateral sinus narrowing as the most common abnormal findings in magnetic resonance venogram.