Antibodies, Antiphospholipid ; immunology ; therapeutic use ; Antiphospholipid Syndrome ; diagnosis ; immunology ; therapy ; Child ; Humans

OBJECTIVE To study the influence of various packing methods and controlling method toward the hidden wet pack after steam sterilization to ensure the sterilization quality and hygiene safety.METHODS The medical equipment was packed with three types of methods,then unloaded from the steam autoclave after being cooled at different time to observe their heating speed respectively.To choose two types of cotton fabric packaging methods,prolong the heating time,and cool in the steam autoclave for 30 minutes,then observe the heating speed of the inside pack and the dry qualification status after 12 hours respectively.RESULTS The heating speed was the fastest with the medical crinkly paper method;the heating speed of the inside pack didn't have any improvement after prolonging the heating time;the dry qualification rate had distinct difference between the two types of cotton fabric methods after 12 hours(P

Carbazoles ; therapeutic use ; Child, Preschool ; Heart Failure ; complications ; drug therapy ; Humans ; Male ; Propanolamines ; therapeutic use ; Takayasu Arteritis ; complications ; drug therapy ; Vasodilator Agents ; therapeutic use

Object To investigate the effect of total flavone in Sanguis Draconis (tFSD) on platelet aggregation, thrombus formation and myocardial ischemia in rat and rabbit. Methods Adopting the experimental models including in vitro platelet aggregation in rat and rabbit, in vivo thrombus formation in rat internal vein, and acute myocardial ischemia by ligating the coronary artery. Results In vitro, tFSD could, to a certain degree, inhibit the platelet aggregation induced by ADP and PAF. Moreover, tFSD could markedly restrain artificial thrombus formation in rat internal vein and the area of acute myocardial ischemia after ligating the coronary artery. Conclusion tFSD has the function of promoting blood circulation and removing blood stasis, anti thrombosis, together with platelet aggregation inhibition.

After the history of health-promoting Qigong was described, the collation and digitalization plan of Qigong in formulas or rhymes was put forward, then a Qigong in formulas or rhymes database was constructed based on their indexing, the health preserving and cultural value of Qigong in formulas or rhymes was mined.

Objective To explore the protective efficacy of antioxidants (vitamin E and Ginkgo biloba extract) on the peripheral nerve damages induced by antiepileptic drugs (AEDs). Methods Adult (2-month old) and infant SD rats (7-day old) were respectively treated with AEDs (phenytoin,or phenobarbital,or clonazepam) alone,or simultaneously with vitamin E or Ginkgo biloba extract for 4 weeks. All the rats were sacrificed,sciatic nerves and serum were collected. The sciatic nerves were analyzed by histologically for their pathological changes,and serum and homogenate of sciatic nerves were investigated for their total antioxidative capacity and antioxidant enzyme activity. Results Incidence of pathological abnormalities of teased fibers significantly reduced in all rats treated with AEDs and antioxidants (P

Objective To explore the clinical usage of allograft vessels for portal venous reconstruction during radical resection for pancreatic cancer.Methods We retrospectively analyzed clinical data of 14 patients who underwent pancreaticoduodenectomy with vascular resection (PDVR) and reconstruction by allograft vascular form June 2009 to May 2013.Results Patient's mean age was (59.1 ±10.4) years old.The mean diameter of tumors was (3.9 ± 1.3) cm,mean lcngth of resected vessels was (4.4 ±0.5)cm,and the R0 resection rate was 71.4％.The implanted vessels in 4 cases were allograft portal veins,and in the other 10 cases were allograft iliac arteries.In morphological classification,9 cases used straight vessels and 5 cases used Y shaped vessels.The overall median survival time of the group was (14.0 ± 3.0) months.The 1,2 year' s survival rate was 52.0％ and 40.0％,respectively.Conclusions Allograft vessels for portal venous reconstruction during PDVR have biological and structural advantages.

AlM: To investigate the effect of endocapsular phacoemulsification cataract extraction and intraocular lens (lOL) implantation with a 1. 8mm or 3. 0mm clear corneal incision on total root mean square ( RMS ) value of the cornea, corneal astigmatism, spherical aberration, coma, trefoil and tear film.
METHODS:ln a prospective study, 156 age- related patients ( 196 eyes ) were randomly distributed into two groups. 1. 8mm-group comprised 94 eyes that had a silicone lOL inserted through a 1. 8mm sutureless clear corneal incision, while, 3. 0mm- group comprised 102 eyes through a 3. 0mm clear corneal incision. Postoperatively, the changes in the total RMS value of the cornea, corneal astigmatism, spherical aberration, coma, trefoil and tear film at 1wk, 1 and 3mo were determined respectively.
RESULTS:ln both groups, postoperatively at 1wk,there were statistically significant differences ( P<0. 05 ) in the total RMS value of the cornea, corneal astigmatism, spherical aberration, coma, trefoil and tear film, while, there were statistically minimal differences ( P< 0. 05 ) between 1. 8mm-group and 3. 0mm-group at 1mo, but were not statistically significantly different ( P > 0. 05 ) between two groups at 3mo postoperative.
CONCLUSlON:This study confirms that incision size has strong impact on the corneal higher-order aberrations, especially, 3. 0mm incision caused significant differences in the total RMS value of cornea, corneal astigmatism, spherical aberration, coma, trefoil and tear film compared with 1. 8mm micro-incision, therefore, micro-incision is very beneficial for clinical use in phacoemulsification.

Objective To investigate the pathological features of sural nerve biopsy in patients with motor neuron disease （MND）. Methods 22 patients with amyotrophic lateral sclerosis (ALS) underwent sural nerve biopsy and routine electrophysiological examination. The transected images were captured and morphometrically analyzed.Results The myelinated fiber density decreased in ALS patients' sural nerves, and larger fibers were involved mostly. The thinly myelinated fibers increased. Conclusion The sural nerves of ALS patients shows mild but definite pathological changes.

Objective To investigate the clonal types of Staphylococcus aureus collected from 2004to 2010 in patients with blood stream infection from a Grade A tertiary care hospital in Shanghai as well as the dynamic changes and to detect the variation in antimicrobial resistance and virulence-gene content in different strain types.Methods A total of 103 nonduplicate S.aureus isolates were collected from 2004 to 2010 from inpatients with S.aureus blood stream infection from Shanghai Huashan hospital.Determination of oxacillin MICs and the type of SCCmec gene were used to screen MRSA.Typing of S.aureus isolates was identified by using multilocus sequence typing(MLST) and S.aureus-specific staphylococcal protein A typing(spa typing),PCR was used to detect the antimicrobial resistance and virulence-gene.Results Sixtysix isolates(64.1%) MRSA were detected in 103 nonduplicate S.aureus isolates,and 35 isolates were MRSA with SCCmec type Ⅱ ,Twenty-nine isolates were MRSA with SCCmec type Ⅱ,two isolates were MRSA with SCCmec type Ⅳ,Thirty-seven isolates(35.9%) were MSSA.Thirty-three MRSA isolates were ST5,Twenty-nine MRSA isolates were ST239,two MRSA isolates were ST59,one MRSA isolates was ST641 and one MRSA isolates was ST6.All of the other clones belonged to MSSA.The percentage of ST5 and ST239 were decreased significantly after 2009(ST5 was decreased from 52.9% to 15.4%; ST239 was decreased from 61.1% to 15.4%),and new clonal types MSSA increased significantly(in 2009,the percentage of ST7 was 41.7%; new clonal types such as ST188 and ST15 were detected in 2010).In 2010,it was shown that 84.6% of MSSA were isolated from S.aureus blood stream infection,nineteen isolates(18.4%) harbored mupA gene and 41 isolates(39.8%) harbored qacA/B gene in 103 nonduplicate S.aureus isolates.It was shown that 70.6% ST239 harbored qacA/B gene.Four isolates of ST398 and 1 isolates of ST9were detected which were originally from animal.There was no significant difference of the virulence gene presence in the same strain types except sasX、lukSF and arcA genes,but there were a lot of genes which were restricted to different genomic background.Conclusions The percentage of ST5 and ST239 were decreased and new clonal types of MSSA were increased significantly in S.aureus blood stream infection,antimicrobial resistance and virulence-gene were restricted to different clonal types.