1.The Effect of Pravastatin on Impaired Endothelium-dependent Relaxation of Isolated Rabbit Aortic Rings Induced by Homocysteine
Journal of Chinese Physician 2000;0(12):-
Objective To explore whether pravastatin protects endothelium against the damage induced by homocysteine (Hcy) in isolated rabbit aorta. Methods Endothelium-dependent relaxation responses to acetylcholine (ACh) of thoracic aortic rings were measured by isometric tension recording before and after aortic rings exposed to Hcy in the absence or presence of pravastatin (PT) to estimate the injury effect of Hcy and the protective effect of pravatatin on rabbit aortic endothelium, respectively. Results Incubation of aortic rings with 1~10mmol/L Hcy for 30min significantly inhibited endothelium-dependent relaxation response to ACh of aortic rings in a concentration-dependent manner. Pre-incubation of aortic rings with 0.3~3mmol/L PT for 15min and co-incubation of aortic rings with 3mmol/L Hcy for another 30min markedly attenuated the inhibition of endothelium-dependent relaxation induced by Hcy in a concentration-dependent manner. Conclusion Pravastatin can improve the impairment of endothelium-dependent relaxation induced by Hcy in isolated rabbit aorta.
3.Clinical observation of diclofenac sodium eye drops for postoperative recurrence of pterygium
Zhi-Rong, XU ; Hao, YAN ; Mei, FENG
International Eye Science 2014;(9):1712-1714
To observe the clinical efficacy of diclofenac sodium eye drops for postoperative recurrence of pterygium, and to provide safer drug regimens to prevent the recurrence of pterygium.
● METHODS: From November 2011 to September 2013, 124 patients 134 eyes who underwent surgery because of pterygium in our hospital were randomly divided into trial group ( diclofenac sodium eye drops and sodium hyaluronate eye drops) and control group ( tobramycin dexamethasone eye drops and sodium hyaluronate eye drops), all patients were followed up for 3mo ( once a week ) and the cornea, intraocular pressure and recurrence of pterygium were observed and compared between the two groups.
●RESULTS: Followed up for 3mo, 43 cases (45 eyes) in the trial group and 38 cases (39 eyes) in the control group had done the follow up as planned, in which 14 cases (15 eyes) showed the signs of recurrence, 2 cases (3 eyes) recurred eventually in the trial group, and in the meantime there were 12 cases (13 eyes) with signs of recurrence and 2 cases ( 2 eyes ) with a recurrence eventually in the control group, but no statistical difference was found between the two groups (P>0. 05);there were 6 cases (9 eyes) dropped out of the study with a higher intraocular pressure in the control group, compared to trial group (0 cases of 0 eye), the difference was statistically significant ( P < 0. 05); cornea conditions between the two groups had no statistical significance (P>0. 05).
●CONCLUSlON:Diclofenac sodium eye drops is effective and safe in preventing the recurrence of pterygium.
4.Relationship between alexithymia and event-related potential P300 in patients with somatoform disorders
Feng YI ; Jia MEI ; Jingyu MAO ; Xujiang SU ; Yan WANG
Chinese Journal of Physical Medicine and Rehabilitation 2013;35(10):810-813
Objective To investigate the relationship between alexithymia and event-related potential P300 in patients with somatoform disorders.Methods Forty-two patients aged 18 to 65 years fulfilling ICD-10 diagnosis criteria for somatoform disorders were recruited as experiment group.Forty normal healthy persons were selected as control group.The Toronto alexthymia scale-20 (TAS-20) was employed to investigate the alexithymia of the subjects.P300 potentials was employed to investigate the cognitive ability of the subjects.A comparison was made between the patients and the healthy subjects.Results The latencies of N1,P2,N2 and P3 were significantly longer in patients with somatoform disorder than those in the normal controls (P < 0.05 or 0.01),the amplitudes of N2 and P3 were significantly lower in the patients (P < 0.05 or 0.01).The three factors scores and the total score of TAS-20 test in patients with somatoform disorder were significantly lower than those in the normal controls (P < 0.05 or 0.01).There was significant correlation between the latency and the three factors scores and the total score of TAS-20 as well as between the amplitude of P300 and the three factors scores and the total score of TAS-20:the latencies of N1,P2,N2 and P3 recorded at Pz was positively correlated with the factors scores (r =0.32 ~ 0.48,P < 0.05 or 0.01),and the amplitudes of N1,P2,N2 and P3 recorded at Pz was negatively correlated with theTAS-20 scores (r=-0.31 ~-0.51,P<0.05 or0.01).Conclusions There existed significant correlation between the alexithymia and cognitive impairment in patients with somatoform disorders.Both alexithymia and event-related potential P300 can be used as a clinical evaluation index for patients with somatoform disorder.
7.Advances in lymphangiogenesis and metastasis of tumor.
Xiao-chu YAN ; Dong-mei YU ; Feng-xuan LIU
Chinese Journal of Pathology 2005;34(6):370-372
Animals
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Humans
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Lymph Nodes
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pathology
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Lymphangiogenesis
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physiology
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Lymphatic Metastasis
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Lymphatic Vessels
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metabolism
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pathology
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Neoplasms
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metabolism
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pathology
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Vascular Endothelial Growth Factor C
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metabolism
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physiology
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Vascular Endothelial Growth Factor D
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metabolism
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physiology
8.Simultaneous determination of four anthraquinones in rat plasma by HPLC-FLD method and its pharmacokinetic study.
Jin-Qiu HUANG ; Xue-Mei YAN ; Fang FENG
China Journal of Chinese Materia Medica 2014;39(19):3850-3854
This paper aims to develop a method for the determination of aloe-emodin, rhein, chrysophanol and physcion and study the pharmacokinetic properties of four anthraquinones in rat plasma after oral administration of gardenia and rhubarb decoction. The plasma concentrations at different time points of four anthraquinones were determined by HPLC-FLD method. Plasma samples were extracted with liquid-liquid extraction procedure. Plasma samples were separated on a C18 column (4.6 mm x 150 mm, 5 μm), using 0.2% acetic acid and methanol as mobile phase at a flow rate of 1.0 mL min(-1) with gradient elution. The excitation and emission wavelengths were set at 430, 525 nm, respectively. DAS 2.0 software was applied to calculate the pharmacokinetic parameters. The results showed four anthraquinones can be absorbed. The main parameters of aloe-emodin, rhein, chrysophanol and physcion were as follows: C(max) for aloe-emodin was (0.085 ± 0.058), (3.772 ± 1.152), (0.464 ± 0.267), (0.028 ± 0.008) mg x L(-1) respectively; t(max) for rhein was (1.042 ± 0.510), (0.805 ± 0.307), (1.167 ± 0.283), (0.616 ± 0.162) h respectively; t½ for chrysophanol was (3.557 ± 1.250), (6.879 ± 1.126), (5.196 ± 2.032), (4.337 ± 1.816) h; AUC(0-t) for physcion was (0.504 ± 0.130), (9.558 ± 1.106), (2.545 ± 1.554), (0.052 ± 0.018) mg x h x L(-1). This paper developed a selective, accurate and sensitive HPLC-FLD method for the simultaneous determination of four anthraquiones in rat plasma.
Animals
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Anthraquinones
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blood
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pharmacokinetics
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Chromatography, High Pressure Liquid
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instrumentation
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methods
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Drugs, Chinese Herbal
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analysis
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pharmacokinetics
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Male
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Rats
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Rats, Sprague-Dawley
9.Serotonin system and post-stroke depression
Feng MEI ; Shuyin SUN ; Zuoji ZHANG ; Zhongrui YAN
Chinese Journal of Behavioral Medicine and Brain Science 2015;24(4):360-363
Objective To investigate the effect of the 5-hydroxytryptamine (5-HT) on the occurrence,development of post-stroke depression (PSD).Methods The literatures on the relationship between 5-HT and PSD were selected from databases such as Wanfang Data,CNKI and PubMed before in 2010-2014.According to absorption and exclusion criteria of the literature,collect literatures,and summary,induce and analyze on them.Results Search 258 literatures and accept 44 literatures accorded with the criteria,including the relationship between 5-HT and PSD,the relationship between 5-hydroxytryptamine receptor (5-HTR) gene polymorphism and PSD,the relationship between 5-hydroxytryptamine transporter linked promoter region (5-HTTLPR) and PSD,and the treatment of selective serotonin reuptake inhibitors (SSRIs) in PSD.Conclusion Reduced levels of 5-HT lead to the occurrence of PSD,and they had a negative correlation;5-HTR 1 A,5-HTR2A are related genes of PSD;S allele and S/S genotype is susceptible factors of PSD,while the L allele is the protective factors of PSD;SSRIs can increase the level of 5-HT in the synaptic gap so as to improve the depressive symptoms of PSD.In the future,it is necessary to increase the sample size to confirm the relationship between 5-HT and PSD,and combine with other factors to explore the relationship between them.
10.Effects of patient-related and stroke-related factors on post-stroke aphasia recovery
Yanlong ZHAO ; Zhongrui YAN ; Zuoji ZHANG ; Feng MEI ; Shuyin SUN
Chinese Journal of Behavioral Medicine and Brain Science 2016;25(7):667-670
Post-stroke aphasia is an acquired language disorder caused by stroke.It impaired the quality of life of patients and brought a heavy burden to family and the society.Unfortunately,the highly variable predictive factors make the prognosis of aphasia recovery difficult.Much of the researches indicated that the post-stroke aphasia recovery is associated with the patient-related and stroke-related factors.We searched the recent advances on the influence of patient-related factors and stroke-related factors on aphasia recovery.It showed that patient-related factors have no obvious effect on predicting aphasia recovery while the lesion (stroke)-related factors appeared close correlation with post-stroke aphasia recovery.The clinicians should pay more attention on lesion (stroke)-related factors when evaluate the outcome and give the intervention measures.