BACKGROUND:Repair of large-area burns and severe post-traumatic skin defects has always been urgent clinical breakthrough technology bottleneck. With the development of tissue engineering, epidermal stem cel s are increasingly being used in tissue engineering, cel replacement therapy and genetic engineering. Therefore, the isolation and identification of epidermal stem cel s is becoming the research focus of concern.
OBJECTIVE:To review the research progress in biological markers of epidermal stem cel s.
METHODS:The Chinese Biomedical Literature database, CNKI database, China Academic Journals Ful-text database, PubMed database and EMbase database were retrieved for articles about specific markers of epidermal stem cel s using the keywords of“epidermal stem cel s, integrin, keratin, P63, CD71, telomerase, ACE, cx43, hoechst”in Chinese and English. Older theoretical perspectives and repetitive research were excluded.
RESULTS AND CONCLUSION:Final y, only 40 articles were included in result analysis. Epidermal stem cel s bring a new source for skin tissue repair. Epidermal stem cel s distribute in the fol icle eminence and basal layer of the epidermis. About 4%cel s in the basal layer, however, are stem cel s. Therefore, it is critical to correctly isolate, culture and identify skin stem cel s. As a reason, specific markers of epidermal stem cel s become a hotspot. Currently, a great progress in the biological markers of epidermal stem cel s has been made, but there is stil no absolute and proven marker for epidermal stem cel s. Most studies are focusing on integrin, keratin, P63, CD71, connexin 43, and telomerase. In addition, hoechst, CD90, CD98, CD200 have been reported recently. Each marker has its own shortcomings, and there are stil many problems that need to be solved.

OBJECTIVEMedicinal properties are the basic attribute of traditional Chinese medicines (TCM), while the medicinal property theory is the core theoretical foundation of TCM formula combination. In this particle, authors studied the characteristics of pharmacological effects of property combination of traditional Chinese medicines distributing along meridians, with the aim to introduce the medicinal property combination regularity into the design and optimization process of compound TCMs, and bring the medicinal property theory into full play in guiding the formula combination.

METHODIn this paper, TCMs distributing along "the lung meridian" was taken for example. The medicinal property combinations of TCMs distributing along "the lung meridian" recorded in Pharmacopeia (2010) was collected and processed. Besides, Chinese journal full-text database (CNKI) was used to collect all of pharmacological study literatures concerning the above TCMs that have been published since 1980. The pharmacological information was also supplemented by reference to Science of Chinese Materia Medica and Clinical Science of Chinese Materia Medica.

RESULTTCMs distributing along the lung meridian with different properties and tastes showed significant differences in pharmacological effects. For example, mild-sweet-lung medicines could lower blood sugar levels, decrease anoxia and enhance immunity; Mild-bitter-lung medicines showed anti-bacterial, anti-hypertension, anti-oxidation effects; Hot-sweet-lung medicines showed antibechic and anti-bacterial effects. And Hot-bitter-lung medicines showed phlegm eliminating and anti-inflammatory effects. Meanwhile, TCMs distributing along the lung meridian had similar pharmacological characteristics, such as anti-bacterial and anti-inflammatory effects, which is consistent with lung's feature in susceptibility to exogenous pathogenic factors.

CONCLUSIONIn this study, authors discovered pharmacological characteristics of different TCMs distributing along the lung meridian, which links TCM theory with modern study achievements and lays a foundation for establishing a TCM property formula combination system.

Databases, Factual ; Drugs, Chinese Herbal ; administration & dosage ; chemistry ; Humans ; Lung ; drug effects ; Lung Diseases ; drug therapy ; Meridians

Objective To assess the clinical efficacy of Shenmai injection for the treatment of anaphylactic shock patients and to observe the changes of microcirculation, blood pressure, and volume of urine. Methods Sixty cases of anaphylactic shock were treated with Shenmai injection plus Western medicine (treatment group) and 36 cases of anaphylactic shock treated with Western medicine only(control group). Hemorheological changes, consciousness, temperature, pulse and NSPO 2 were compared. Results The blood pressure recoverable rate of anaphylactic shock in the treatment group was significantly higher than that in the control group. The blood pressure recoverable rates at 4 h after the therapy were 68.3% and 22.2% in the treatment group and the control group, respectively. The average urine volume of treatment group at 2, 3, 4, 5 and 6 h after the therapy was significantly higher than that of the control group ( P

OBJECTIVETo establish a characterization system of traditional Chinese medicinal properties in line with modern scientific cognition regularity, in order to reveal properties of traditional Chinese medicines distributed along liver meridian and relations of effects of medicinal properties.

METHODBy collecting data about traditional Chinese medicinal properties recorded in the Pharmacopoeia of the People's Republic of China (2005 Edition), literature and data about pharmacological effects of traditional Chinese medicines recorded in the Chinese Materia Medica, by using the method of association rules, the authors dug pharmacological effect rules corresponds to relevant medicinal property combinations, with the medicinal property combination of traditional Chinese medicines distributed along liver meridian as the target.

RESULTIt was found that either obvious different pharmacological effects or identical pharmacological characteristics existed in traditional Chinese medicines distributed along liver meridian.

CONCLUSIONWith the aim to explore the correlations between traditional Chinese medicine medicinal properties and pharmacological effects, the authors linked the traditional Chinese medicine theory with modern research achievements, in order to provide the ideas and methods for interpreting mechanisms of medicinal properties.

Databases, Factual ; Drugs, Chinese Herbal ; chemistry ; therapeutic use ; Humans ; Liver ; drug effects ; Liver Diseases ; drug therapy ; Meridians

Medicinal properties are specific attributes of traditional Chinese medicines(TCM). The medicinal property theory is an important principle for the compatibility of traditional Chinese medicines. In this paper, medicinal properties, flavors and meridian tropism were combined to represent TCM medicinal properties; and multiple medicinal properties were further combined into medicinal property combination modes. TCMs and medicinal property combination modes were divided according to their efficacies, which were regarded as the concept of inductive logic programming and finally got medicinal property combination and compatibility rules with different efficacies. These medicinal property combination and compatibility rules were used to form the theoretical model through the entity grammar system, realize the automatic reasoning process from the medicinal property combination and compatibility to the efficacies, verify the reasoning result and analyze their rationality and limitations, in order to provide new ideas for revealing the relations between the TCM compatibility rules and efficacies.
Drug Interactions ; Drug Therapy, Combination ; methods ; standards ; Drugs, Chinese Herbal ; chemistry ; therapeutic use ; Humans ; Meridians ; Models, Theoretical

Objective To investigate the necessity of dilution in samples with high concentrations of D-Dimer and optimum dilution multiple.Methods Quality control products and calibration were detected by using Sysmex CS5100 for precision evaluation,including within-batch and between-run precision.Calibration were detected for validation of linear range and clinical reportable.Samples with D-Dimer<5 mg/L and fibrinogen degradation products(FDP)<20 μg/mL were serially diluted and detected to calculate recovery rate.Samples with D-Dimer>5 mg/L and FDP>20 μg/mL were also serially diluted and detected to calculated recovery rate.Results Within-batch and between-run coefficients of variation were both less than 3%.Within the scope of 0.207-5.170 mg/L,the linear distribution was fine.The clinical reportable range was 0.207-165.440 mg/L.For samples with D-Dimer<5 mg/L and FDP<20 μg/mL,no antigen excess phenomenon was found,and gradient dilution was not necessary.For samples with D-Dimer>5 mg/L and FDP>20 μg/mL,there was obvious antigen excess phenomenon,and gradient dilution was required.Conclusion For samples with D-Dimer>5 mg/L and FDP>20 μg/mL,dilution should be performed to ensure the accuracy of detected results.

Objective To evaluate the value of peripheral blood neutrophil CD_(64) level for early diagnosis of neonatal septicemia.Methods Thirty-six cases of neonatal septicemia were recruited into the study according to diagnostic criteria for neonatal septicemia:white blood cell count,C-reactive protein(CRP),platelet count,micro-erythrocyte sedimentatio rate(mESR) and blood culture;26 cases of hospitalized neonates with non-infectious diseases were enrolled as controls.The level of peripheral blood neutrophil CD_(64) was measured by flow cytometry.Results The level of peripheral blood neutrophil CD_(64) in septicemia patients were(60.37?22.70)mean fluorescence intensity(MFI),which were significantly higher than that of control group(23.14?5.10)MFI(P

Objective To evaluate the method and result of individual deglutition training on dysphagia after Stroke. Methods 36 patients with dysphagia after stroke were randomized into observation group (20 patients receiving individual deglutition training and routine therapy of neurology) and control group (16 patients receiving basal deglutition training and routine therapy of neurology).Results After treatment for 4 weeks, the score of dysphagia in observation group were higher than in control group (P<0.05). Conclusion Individual deglutition training could significantly improve the swallowing function of the patients with dysphagia after stroke.

OBJECTIVEThe efficiency network is a complicated network for revealing the efficient mechanism of traditional Chinese medicines (TCMs) and relations among efficiencies. The efficiency-property relations were used to establish a warm-pungent-liver efficiency network to explain the principle of treating hepatoma with medicines invigorating blood circulation and eliminating blood stasis. Safflower, a warm-pungent medicine distributing along the live meridian, was taken for example to discuss the efficiency network' s application in the identification of active ingredients of TCMs and the combination.

METHODIn the early stage of this study, combined warm-pungent-liver medicines distributed along the liver meridian and invigorating blood circulation and eliminating blood stasis were taken as the study objects to collect the pharmacological effect data of warm-pungent-liver medicines and obtain the pharmacological effect combinations with the highest blood circulation-invigorating association by the association rules and the chi-square test. The pharmacological target data recorded in the DrugBank database is used to establish the warm-pungent-liver efficiency network according to the principle line of "efficiency-property-pharmacology-target-protein interaction" under the background of the protein interaction network.

RESULTThe blood circulation-invigorating medicines could directly treat hepatoma by impacting protooncogene, cancer suppressor gene, cell apoptosis and anti-inflammation, and indirectly treat hepatoma by resisting coagulation and adhesion, regulating local blood circulation, preventing cancer cell metastasis and enhancing the tissues' sensitivity to the anticancer drugs. Among the active ingredients of safflower screened based on the blood circulation-invigorating network targets, carthamin yellow, quercetin and luteolin have been proved to have the anti-hepatoma effect in literatures, which indicated the reliability of this study's results and the purpose of the efficiency network.

CONCLUSIONThe efficiency network is an effective method for revealing the TCM's mechanism, and lays a foundation for discovering key active ingredients of TCMs for treating specific diseases.

Antineoplastic Agents, Phytogenic ; chemistry ; therapeutic use ; Blood Circulation ; drug effects ; Carcinoma, Hepatocellular ; drug therapy ; genetics ; metabolism ; physiopathology ; Drugs, Chinese Herbal ; chemistry ; therapeutic use ; Gene Regulatory Networks ; drug effects ; Humans ; Liver ; blood supply ; drug effects ; Liver Neoplasms ; drug therapy ; genetics ; metabolism ; physiopathology

OBJECTIVETo study topiramate's new functions according to the medicinal property combinations, in order to apply the traditional Chinese medicinal theory in discovering new purposes of old drugs.

METHODAccording to New Traditional Chinese Medicinal Families--Chemical Traditional Chinese Medicines, the authors found out topiramate's property. Then based on the therapeutic principle of diabetes, hypertension, epilepsy and lung cancer, as well as the relations of efficacies and medicinal property combinations, they summarized the corresponding medicinal property combination modes, compared topiramate's medicinal property combination mode with corresponding medicinal property combination modes of these diseases, and predict topiramate's new functions.

RESULTAccording to the comparison, the corresponding medicinal property combinations were consistent with topiramate's medicinal property combinations as evidenced by corresponding literatures, whereas other medicinal property combinations were not.

CONCLUSIONBased on medicinal property combination modes, the authors screened topiramate's new functions according to e of TCM clinical experience, discovered topiramate's therapeutic effects on diabetes, hypertension and lung cancer in addition to epilepsy, and explore new drug function according to medicinal property combination modes, which could help greatly shorten the new drug R&D period.

Diabetes Mellitus ; drug therapy ; Drug Therapy, Combination ; Drugs, Chinese Herbal ; chemistry ; therapeutic use ; Fructose ; analogs & derivatives ; chemistry ; therapeutic use ; Humans ; Hypertension ; drug therapy ; Lung Neoplasms ; drug therapy ; Phytotherapy