Adult ; Case-Control Studies ; Humans ; Male ; Middle Aged ; Pneumoconiosis ; blood ; immunology ; T-Lymphocyte Subsets

Objective:To investigate the effects of the psychological stress on peripheral blood lymphocytes apoptosis and Bax,Bcl-2 gene expression in rats after being given different intensity of noise stress.Methods:All 24 SD rats were divided into three groups:control,high intensity of the noise stress,middle intensity of the noise stress. The apoptosis in peripheral blood lymphocytes(PBL)of rats was demonstrated with the flow cytometer to determine the percentages of apoptosis in rat PBL,and the expression changes of related Bcl-2,Bax genes were observed by us- ing immunohistochemical method.Results:1)After high intensity of the noise stress for three weeks,the apoptosis in rat PBL increased significantly compared with the control.2)After high intensity of the noise stress for three weeks, Bcl-2 expression as down-regulation in PBL,but the B ax expression remained obviously high.Conclusion:It indi- cates that apoptosis exists in the immunocytes of rats in the course of high intensity of the psychological stress and it consists with the expression of the related regulatory genes.

Hemoperfusion ; Humans ; Metabolic Clearance Rate ; Paraquat ; blood ; pharmacokinetics

Blood Cells ; immunology ; pathology ; Bone Marrow Cells ; immunology ; pathology ; Child, Preschool ; Diagnosis, Differential ; Female ; Humans ; Immunophenotyping ; Leukemoid Reaction ; diagnosis

AIM: To observe the clinical effect of phacoemulsification combined with intraocular lens implantation for high myopia ( axis oculi ≥ 30mm ) cataract, and to explore the causes and treatment methods of operation complications.
METHODS: Selected 64 cases ( 86 eyes ) of cataract with high myopia patients (axis oculi ≥30mm), vision correction≤ 0. 1. Phacoemulsification combined with intraocular lens implantation was used. During 1a follow-up, the improvement of visual acuity and complications were observed.
RESULTS: Postoperative best corrected visual acuity was ≥0. 4 in 35 eyes (41%), 0. 2-0. 3 in 23 eyes (27%), 0. 1-0. 15 in 25 eyes (29%), <0. 1 in 3 eyes (3%). Posterior capsular rupture, vitreous prolapsed occurred in 2 eyes ( 2%) . Through the anterior vitrectomy, intraocular lens was implanted to ciliary sulcus. Postoperative corneal edema in 6 eyes (7%), which was subsided after treatment; Posterior capsular opacity in 9 eyes (10%), visual acuity was recovered after incision of posterior capsule by YAG laser. Retinal detachment in 1 eyes (1%), which was cured after sclera buckling.
CONCLUSION: Phacoemulsification combined with intraocular lens implantation is a safe and ideal operation method for high myopia cataract, can obtain good effects, and the postoperative complications can be controlled. Because of the particularity of high myopia, operation should be gentle, technical parameters should be adjusted as appropriate, to achieve the best postoperative effects.

Objective To investigate the effects of Leptin on the base of anthropometry,densitometry,and biochemistry and reveal the role of Leptin in bone metabolism in postmenopausal women with osteoporosis (OP).Methods From January 2008 to January 2014,data of 82 postmenopausal females with or without OP were analyzed.They were randomized into two groups:OP (n=40) and conditional control group (CON) (n=42).The expression of Leptin receptors in osteoblasts was observed by immunohistochemistry.1 × 10 ng/ml,1 × 102 ng/ml,1 × 103 ng/ml,1× 104 ng/ml of Leptin were added respectively.Then the proliferation,differentiation and mineralization of osteoblasts were measure at 24 h,48 h and 96 h.The gene expressions of bone marrow mesenchymal stem cells (BMSCs) derived osteoblasts and adipocytes treated with Leptin were examined by quantitative analysis of Real time-PCR.Results The expression of Leptin receptors in osteoblasts was observed on plasma membranes and cytoplasm in the normal group and osteoporosis group.In osteoporosis group,different concentrations of Leptin enhanced cell proliferation,cell differentiation and cell mineralization.The growth rate of MTT and the concentration of ALP in the serum of were significantly increased with the effects of 1×10 ng/ml,1×102 ng/ml and 1×103 ng/ml of Leptin,and the action of 1×102 ng/ml Leptin was the most powerful with time dependence.Within 96 hours,the growth rate of osteoblasts increased gradually,and the concentration of ALP increased gradually in 3 weeks.The expression of RANKL/OPG in bone marrow BMSCs derived osteoblasts treated with Leptin were significantly increased.Conclusion Leptin receptor is present in osteoblasts,and Leptin affects biological behaviors of osteoblasts through receptors.The direct effect of Leptin may relate the expression of RANKL/OPG.Then,the balance between bone resorption and bone formation was broken and finally osteoporosis occurred.Lepin may promote osteogenesis and inhibit bone resorption in the differentiation of BMSCs.

OBJECTIVE:To inverstigate the inhibitory effects of 14 kinds of traditional Chinese medicines on tyrosinase activity METHODS:The effective components of traditional Chinese medicines were extracted by 50% ethanol The speed of enzymic catalysis of tyrosinase was determined by ultraviolet spectrophotometry to evaluate the inhibitory effects of extracts of traditional Chinese medicines RESULTS:14 reactive time curves of enzymic catalysis of tyrosinase were obtained CONCLUS_ION:13 kinds of traditional Chinese medicines in vitro showed a better inhibitive effect on tyrosinase activity(P

Objective To explore the change of serum level of neuron specific enolase (NSE) in neonates with asphyxia before and after head mild hypothermia.Methods Eighty-two asphyxial neonates were selected,including 39 mild asphyxial neonates and 43 severe asphy-xial neonates,and 29 healthy neonates were selected as control group.Forty-three severe asphyxial neonates were randomly assigned into mild hypothermia treatment group and traditional treatment group.Neonates in traditional treatment group were just given traditional treatment.While neonates in mild hypothermia treatment group received head mild hypothermia therapy and their nasopharyngeal temperature were maintained at(34.0 ? 0.5) ℃ for 72 h.Before treatment and 72 h after treatment,2 mL blood was collected,and the serum NSE was determined by radio immunoassay.Results NSE levels in mild asphyxial neonates group[(34.83?6.17) ?g/L] and severe asphyxial group[(59.58?8.87) ?g/L] were significantly higher than that of control group[(30.57?4.88) ?g/L](t=3.07 P0.05).The level of NSE at 72 h in severe asphyxial neonates with head mild hypothermia therapy[(40.97?6.55) ?g/L] was significantly lower than that of traditional treatment group [(48.15?5.57) ?g/L](t=3.86 P

Objective To compare the effects of three different training patterns of MOTOmed training on the somatosensory evoked potentials (SEPs) of healthy youths. Methods Ten healthy young volunteers received training in patterned sequences of passive movement, active movement with no resistance and active movement with resistance. Each pattern lasted for 30 minutes and SEPs were examined before and after 90 minutes of training. The amplitude and latency of N9 and P40 were recorded. Results All three training patterns heightened SEP amplitude and lengthened SEP latency, but active training had the most obvious effect on amplitude. The rates of change of am-plitude after each training pattern had significant differences, which was most obvious after training the resistance training pattern. Conclusions MOTOmed motor training can excite the cerebral cortex and up-regulate SEP ampli-tude. Active movement with resistance is the most effective among the three patterns tested.

Objective To analysis curative effect of triple therapy and sequential therapy for eradication of Helicobacter pylori in patients with long-term aspirin use.Methods 52 patients with long-term aspirin use who were diagnosed with helicobacter pylori infection were collected.All patients were divided into sequential group and triple group according to different drugs classified, each 26 cases in each group were given corresponding drug treatment, after the end of treatment, the serum pepsinogen, Hp-IgG antibody levels and Helicobacter pylori clearance rate were detected in all patients. Results After treatment, compared with the triple Group, in the sequential group, the levels of serum PG I and PG II were lower, and PGⅠ/PGⅡwas higher, and the differences were statistically significant (P<0.05);the serum Hp-IgG antibody levels were lower in the sequential group (P<0.05);the Hp clearance rate of patients in the sequential group was higher (P<0.05).Conclusion Compared with triple therapy, sequential therapy can lower serum PG, PG II and Hp-IgG levels, and improve the Hp clearance rate and clinical efficacy, have guiding significance to clinical.