1.Primary pulmonary invasive carcinoma ex pleomorphic adenoma: report of a case.
Bai-zhou LI ; Yan-biao FU ; Guo-feng WANG
Chinese Journal of Pathology 2012;41(1):54-54
Adenoma, Pleomorphic
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metabolism
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pathology
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surgery
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Aged
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Carcinoma
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metabolism
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pathology
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surgery
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Cell Transformation, Neoplastic
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Humans
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Lung Neoplasms
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metabolism
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pathology
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surgery
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Male
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Mucin-1
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metabolism
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S100 Proteins
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metabolism
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Vimentin
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metabolism
2.Research of resisted function of L-arginine on BP in stress rats.
Yan-Ru YIN ; Jin-Feng HAN ; Lin BAI
Chinese Journal of Applied Physiology 2002;18(2):172-185
Animals
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Arginine
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pharmacology
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Blood Pressure
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drug effects
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Hypertension
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etiology
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metabolism
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physiopathology
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Male
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Oxidative Stress
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drug effects
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Rats
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Rats, Sprague-Dawley
3.Treatment of severe hepatitis by plasma exchange
Yu LI ; Xuefan BAI ; Zhihua FENG ; Yan ZHANG
Journal of Medical Postgraduates 2003;0(05):-
Objective:To observe the therapeutic effects of plasma exchange in severe hepatitis. Methods:53 cases served as treatment group and 49 cases as control group. Both groups were similar in basic medical treatment, and an additional treatment of plasma exchange was carried in the treatment group. 186 times of plasmas exchange were performed in the treatment group, to observe and compare the changes in patients of the 2 groups in symptoms、liver functions and survival rates. Results:The clinical symptoms of patients in the treatment group were obviously improved, the liver functions were also obviously improved in the treatment group as compared with the control group, and the survival rate of treatment group was higher than that of control group(73.58% vs 46.94%, P
4.Synovial sarcoma of tongue: report of a case.
Yan-biao FU ; Guo-feng WANG ; Bai-zhou LI
Chinese Journal of Pathology 2010;39(8):557-558
Glossectomy
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methods
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Humans
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In Situ Hybridization, Fluorescence
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Keratins
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metabolism
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Male
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Middle Aged
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Oncogene Proteins, Fusion
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genetics
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Sarcoma, Synovial
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genetics
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metabolism
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pathology
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surgery
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Tongue Neoplasms
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genetics
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metabolism
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pathology
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surgery
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Translocation, Genetic
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Vimentin
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metabolism
5.Relationship between Human Papillomavirus Infection and Expression of p16 in the Lesions of Condyloma Acuminata,Bowenoid Papulosis and Bowen's Disease
Shuping GUO ; Liying CHEN ; Li BAI ; Yan FENG ;
Chinese Journal of Dermatology 1995;0(03):-
ObjectiveToinvestigatetheeffectofhigh-andlow-riskhumanpapillomavirus(HPVs)ontheexpressionofp16proteininthelesionsofcondylomaacuminata(CA),Bowenoidpapulosis(BP)andBowen'sdisease(BD).MethodsHPV6/11,HPV16/18DNAinthelesionswereexaminedwithFQ-PCRmethodin30casesofCAandinnormalskinormucosaof12cases.HPV16DNAweredetectedwithinsituhybridizationmethodin30casesofBPand15casesofBD.P16,Ki-67proteinswereexaminedwithimmunohistochemicalSPmethodinthesamespecimens.ResultsBothp16proteinandKi-67expressionwereincreasedinHPV-infectedlesions(P
6.Processing Technology Research of Fermentation and Purification of SUMO Protease UlP1
Xiu-Ping FENG ; Bai-Rong DU ; Dong-Mei YAN ; Xiang-Feng ZHAO ; Xun ZHU ;
China Biotechnology 2006;0(02):-
Nowadays,small peptides are always expressed in the form of fusion protein.The expression product contains many superfluous amino acids which can affect the biological functions of small peptides even expressed by GST fusion protein expression system.SUMO protease can cut SUMO fusion protein expressed by fusion expression system without any amino acid residues left on target protein thus become a hot topic in this field.Recombinant His-UlP1/pET3c/BL21(DE3)engineering strain was constructed by genetic engineering technology and the expression conditions were optimized in shake flaks.The process of high density fermentation was explored and different purification conditions were detected by chromatography.The results showed that SUMO protease could be expressed well after inducing the engineering strain by IPTG of 1.0mmol/L at 30℃ for 6 hours.The expression level of the strain in fermentation pots could reach 24.3% analyzed by SDS-PAGE.The purity of SUMO protease was more than 98% after further purification by cation exchange chromatography.The yield was 355mg SUMO protease per liter fermentation liquid.Western blot analysis demonstrated that there were immune reactions between IlP1 and 6?His antibodies,so it has established a good foundation for large-scale industralazation in the future.
8.Serum Myeloperoxidase Level in Systemic Lupus Erythematosus.
Ming-jian BAI ; Jing FENG ; Feng YU ; Cun-ling YAN ; Chan-juan CUI ; Lei HUANG ; Zhen-ru FENG
Chinese Medical Sciences Journal 2015;30(3):199-202
Adult
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Female
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Humans
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Lupus Erythematosus, Systemic
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enzymology
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Male
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Middle Aged
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Peroxidase
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blood
9.Huge pneumatocele in a child.
He-Cheng ZHANG ; Yan BAI ; Rong-Feng WANG ; Zheng-Guang CHEN
Chinese Journal of Contemporary Pediatrics 2010;12(2):156-157
10.Effects of aluminum on the intracellular calcium concentration and the expression of calcium channels in the hippocampus of rats
Rui, REN ; Yang, ZHANG ; Xiao-feng, ZHANG ; Yan-ping, WU ; Bai-xiang, LI
Chinese Journal of Endemiology 2009;28(5):501-504
Objective To dynamically investigate the effects of aluminum on the concentration of free intracellular Ca2+([Ca2+]i) and the expression of calcium channels in the hippocampus of rats. Methods Healthy 64 Wistar rats were taken as the experimental objects. And these rats were randomly divided into 16 groups according to their weights, and were instilled with AlCl3 at 0(control),37.3,74.7 and 248.7 mg/kg respectively. The experimental time exposed to AlCl3 was 45,75,120 d, among which the rats were given AlCl3 for 120 d fed normally for 30 d. The hippoeampus were segregated on day 45,75,120 and 150 d and the[Ca2+]i of hippocampus of rats were detected by fluorospectrophotometer. The expression of Ryanodine receptor 2 (RyR2) mRNA and α1C ubunit of L-type calcium ehannels(L-Ca2+α1C) mRNA were detected by RT-PCR analysis. Results [Ca2+]i was increased by AlCl3 in a dose-and time-dependant manner(F=23.136 and 19.089, P<0.01). There was a synergistic effect between the dose and time in [Ca2+]i (F=2.270, P<0.05). In time of 120,150 days, the [Ca2+]i of rats hippocampus in 37.3[(299.3±48.7), (342.7±35.3)nmol/L], 74.7[(391.2±47.9), (408.1±42.8)nmol/L] and 248.7 mg/kg group[(397.9±55.8), (405.2±22.7)nmol/L] significantly increased compared with control group [(195.1±29.9), (209.1±30.6)nmol/L; P<0.01]. The expression of RyR2 mRNA and L-Ca2+α1C mRNA were increased by AlCl3(F=23.301 and 60.812, P<0.01). The experimental time could lower the expression of L-Ca2+ α1C mRNA (F=6.088, P<0.01), but had no influences on the expression of RyR2 mR NA (F=1.361, P>0.05). There was interaction between the dose of AlCl3 and the time in the expression of L-Ca2+α1C mRNA (F=5.876,P< 0.01). On day 75,120 and 150 of the experiment, the expression of L-Ca2+α1C mRNA in rat hippocampus of 74.7 (1.03±0.16,1.18±0.18,0.92±0.11) and 248.7 mg/kg group(1.89±0.26, 1.25±0.10, 1.07±0.14) also increased compared with control group(0.63±0.09,0.78±0.16,0.69±0.11; P<0.05 or <0.01). On day 45,75, 120 and 150 of the experiment, the expression of RyR2 mRNA in 74.7(0.49±0.06,0.51±0.07,0.57±0.11, 0.47±0.11), 248.7(0.47±0.03,0.52±0.09, 0.70±0.10, 0.78±0.09)mg/kg AlCl3 groups was highly increased compared with control group (0.24±0.07, 0.32±0.04, 0.30±0.06, 0.27±0.06; P<0.05 or<0.01). Conclusion Al increases [Ca2+]i by increasing the expression of the RyR2 mRNA and L-Ca2+α1C mRNA, thus exerts an irreversible neuronal toxicity.