1.Study of right ventricular function by echocardiography after lung volume reduction surgery
Chinese Journal of Thoracic and Cardiovascular Surgery 2003;0(06):-
Objective To observe the function changes of right ventricular in chronic obstructive emphysema after lung volume reduction surgery. Methods Twenty-six patients with chronic obstructive emphysema undergoing lung volume reduction surgery were examined by color Doppler echocardiography to determine systolic function and diastolic function of right ventricle in preoperative days and postoperative days of 5~7; 12~14; 1 month and 3 months. Results Contraction of right ventricle: comparing with preoperative parameters, there were significant decrease (P0.05) of those above mentioned in postoperative days 12~14. It recovered at 3 month after operation. Relaxation of right ventricle: the swift filling peak flow-rate in earlier relax period (E) and E flow-rate integra (ETVI) decreased meanwhile swif filling peak flow-rate in late relax period (A) and A flow-rate integra (ATVI) increased in the postoperative days 5~7 (P
2.Prescription Review and Drug Use Rationality Analysis for Pregnant Patients in Outpatient Department of Our Hospital
Yan FANG ; Hengjing CUI ; Wanhua YANG
China Pharmacist 2016;19(3):527-529
Objective:To analyze the situation and problems of prescription medication for pregnant patients in outpatient depart-ment of our hospital in order to improve and ensure the safe and rational drug use. Methods: The prescriptions concerning pregnancy except normal pregnancy monitoring in our outpatient department in 2014 were selected and analyzed and reviewed according to classifi-cation, related regulation and medicine instruction issued by FDA. Results:Totally 882 prescriptions were selected with average varie-ties of (1. 3 ± 0. 1) and sum of (77. 7 ± 0. 2) yuan. Among them, 31 ones were irrational prescriptions accounting for 3. 5%. Con-clusion:In order to promote the rational and safe drug use in pregnant patients, it is necessary to strengthen relevant knowledge educa-tion and prescription review of clinical pharmacist.
3.Ethical Concerns of Psychological Service Work in the Army
Yan LI ; Yunli CHANG ; Fang YANG
Chinese Medical Ethics 2015;(3):466-468
Based on the development of our psychological service in amy , this paper pointed out that our psy-chological services that exist in the following ethical issues:some psychological services staff is competency inade-quacy and lack of professionalism;psychological services exist in the complicated ethical relationship and multiple roles;military secrecy of psychological service in consciousness does not reach the designated position The right of privacy infringement .And according to the above problem is pointed out that our army in the military psychological service work should be:stick to professional ethics , improve professionalism;clear professional role , reducing the recessive harm;improve secrecy consciousness , safeguard privacy rights .
4. Analysis and discrimination of integral structure of Paeoniae Radix Alba and Paeoniae Radix Rubra and their alcohol extracts by infrared spectroscopy
Chinese Traditional and Herbal Drugs 2016;47(19):3508-3512
Objective: The Fourier transform infrared spectroscopy (FT-IR) and two-dimension IR correlation infrared spectroscopy (2D-IR) were used for the identification of Paeoniae Radix Alba and Paeoniae Radix Rubraand their alcohol extracts. Methods: The FT-IR spectra method and 2D-IR correlation spectra method were used. Results: The structural information of samples indicated that Paeoniae Radix Alba and Paeoniae Radix Rubra contained a large amount of calcium oxalate and starch, since some characteristic absorption peaks of the calcium oxalate could be observed; And some characteristic absorption peaks in the range of 950-1 200 cm-1 of the starch can be observed, but their shape and location revealed minor differences. In the secondary derivative infrared spectra (SD-IR), both Paeoniae Radix Alba and Paeoniae Radix Rubra can be observed with the characteristic absorption peaks which in 518/517, 989 cm-1 and so on. Besides, these peaks at 989, 1 015, 1 052, 1 078, 1 105, and 1 161 cm-1 of Paeoniae Radix Rubra, almost of them which are single peak, were stronger than Paeoniae Radix Alba's and the same peaks in Paeoniae Radix Alba were jagged peaks, showing that the difference were related to the contents and structure of starch in Paeoniae Radix Alba and Paeoniae Radix Rubra. The characteristic absorption peaks of the peoniflorin which arouse at 1 716, 1 451, 1 347, 1 277, and 714 cm-1 in the FT-IR spectra of their alcohol extracts can be found. Moreover, the shape and intensity of the peaks were more distinct in the secondary derivative IR spectra of the different parts. For example, in the range of 900-980 cm-1, Paeoniae Radix Alba presented two groups of peaks: 935, 919 cm-1 (strong) and 962, 949 cm-1 (weak), while Paeoniae Radix Rubraonly presented one group of peaks: 941, 920 cm-1 (middle), showing that the difference was related to the content and structure of glucoside in Paeoniae Radix Alba and Paeoniae Radix Rubra. In the 2D-IR spectra, both had five auto-peaks at 887, 968, 1 008, 1 190, and 1 305 cm-1, which were the auto-peaks of glucoside, but the strongest auto-peak of Paeoniae Radix Alba was at 1 190 cm-1 and that of Paeoniae Radix Rubra's was at 968 cm-1. The spectra testified the glucoside compounds in Paeoniae Radix Alba and Paeoniae Radix Rubra were different. Conclusion: A lot of information of Paeoniae Radix Alba and Paeoniae Radix Rubra can be provided by FT-IR spectra method and 2D-IR correlation spectra method which can testify that the content and structure of substance in Paeoniae Radix Alba and Paeoniae Radix Rubra were different and can be used to analyze and distinguish Paeoniae Radix Alba and Paeoniae Radix Rubrapreliminarily which can make a good foundation for further research.
5.Establishment of Caco-2 cell monolayer model with collagen coating 6-well plates for study of traditional Chinese medicine prescription.
Yan-Fang YANG ; Ni WU ; Xiu-Wei YANG
China Journal of Chinese Materia Medica 2014;39(3):515-519
Caco-2 cell monolayer model is widely utilized in drug absorption study and 12-well transwellTM plates were commonly used to study the absorption of different kinds of natural products. To establish a stable method for the study of traditional Chinese medicine prescription, 6-well plates were chosen because of the larger well volumes than 12-well plates. To study the impacts of collagen kinds, coating density as well as coating time on the cell culture, the transepithelial electrical resistance of Caco-2 cell monolayers grown on different collagen coating transwells was determined, and the permeations of propranolol and atenolol as standard markers were detected with HPLC. The results showed that the kinds of collagen, the different coating densities and coating time of rat tail collagen had no significant influences on the Caco-2 cell monolayer integrality and absorption capacity. 6-well plates coated with 2 micro g Scm-2 rat tail collagen for 1 hour were enough reliable and suitable for the study of traditional Chinese medicine prescription in vitro.
Adsorption
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Animals
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Caco-2 Cells
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Cell Culture Techniques
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methods
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Collagen
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chemistry
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Drug Prescriptions
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Humans
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Medicine, Chinese Traditional
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Rats
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Time Factors
6.Comparison of 99Tcm-MIBI SPECT/18F-FDG PET imaging and cardiac MRI in assessment of myocardial injury cases with idiopathic dilated cardiomyopathy
Lei WANG ; Wei FANG ; Chaowu YAN ; Minfu YANG ; Shihua ZHAO
Chinese Journal of Nuclear Medicine and Molecular Imaging 2013;(2):141-145
Objective To evaluate the relationship of myocardial MR contrast delay-enhancement and nuclear perfusion-metabolism pattern in patients with idiopathic dilated cardiomyopathy (IDCM).Methods Forty-two consecutive patients (29 men and 13 women,age:(53 ±12) years) diagnosed clinically with IDCM were enrolled.All patients underwent 99Tcm-MIBI SPECT,18F-FDG PET imaging and MR contrast delay-enhancement imaging within 3-7 d.The myocardial perfusion-metabolism segment analysis was performed using a 17-segment model.Segmental 99Tcm-MIBI and 18F-FDG uptakes were scored visually using a 4-grade scoring system (0 =normal uptake,1 =mildly reduced uptake,2 =moderately reduced uptake,3 =severely reduced uptake).Patterns of perfusiorn/metabolism were classified as normal,mismatch,mild-to-moderate match and severe match.Myocardial MR contrast delay-enhancement was classified into 3 categories (non,mid-wall and transmural delay-enhancement).x2 test was used to analyze the differences of perfusion/metabolism patterns among non,mid-wall and transmural delay-enhancement groups and the myocardial MR contrast delay-enhancement incidence among four perfusion/metabolism groups.Resuits Among the 42 patients,myocardial delay-enhancement was present in 18 patients,of which 94.4% (17/18) showed abnormal myocardial perfusion/metabolism patterns and only 33.3 % (8/24) patients without abnormal myocardial delay-enhancement had abnormal myocardial perfusion/metabolism patterns (x2 =15.944,P < 0.001).Perfusion/metabolism patterns varied in three different categories of non,mid-wall and transmural delay-enhancement (x2 =14.276,P < 0.001).The normal peffusion/metabolism pattern proportions in the non,mid-wall and transmural delay-enhancement groups were 86.2% (526/610),71.0% (44/62) and 28.6% (12/42),respectively.The incidence of transmural delay-enhancement (44.4% (12/27)) was significantly higher in segments with severe match than that in the other 3 groups (normal:2.1% (12/582) ; mismatch:18.1% (15/83) ; mild-to-moderate match:13.6% (3/22) ; x2 =112.530,P < 0.001).Conclusions MR contrast delay-enhancement is much more sensitive in detecting moderate fibrosis,while nuclear perfusion-metabolism imaging can detect more impaired but viable myocardium.Combining the two imaging modalities is useful for providing comprehensive evaluations of myocardial injury in patients with IDCM.
7.Effects of intrathecal Ro25-6981 on focal cerebral ischemia-reperfusion injury in rats
Fang YAN ; Yanbing ZHANG ; Lina WANG ; Jianping YANG ; Ying ZHAN
Chinese Journal of Anesthesiology 2011;31(8):977-979
ObjectiveTo investigate the effect of intrathecal (IT) Ro25-6981 (a selective NR2B receptor antagonist) on focal cerebral ischemia-reperfusion (I/R) injury in rats.MethodsSixty healthy male SD rats weighing 280-320 g in which IT catheter was successfully implanted without complication were randomly divided into 4 groups ( n = 15 each):group sham operation (group S) ; group focal cerebral I/R(group I/R) ; group I/R + Ro25-6981 (group Ro) and group I/R+ normal saline (group NS).The right middle cerebral artery was occluded for 2 h with a nylon thread with rounded tip which was inserted into right internal carotid artery and advanced cranially until resistance was met in groups I/R,Ro and NS.In group Ro Ro25-6981 100 μg/10 μl was injected IT at 0,2,23 h of reperfusion,while in group NS NS was injected IT instead of Ro25-6981.Neurological dificit was assessed and scored (0 = no deficit,3 = severe deficit) at 24 h of reperfusion.The infarct size was determined by 2,3,5-triphenyl tetrazolium chloride staining.ResultsIT administration of Ro25-6981 significantly reduced cerebral I/R-induced infarct sized and neurological deficit score in group Ro as compared with groups I/R and NS.ConclusionRo25-6981 injected IT can protect the brain against local cerebral I/R injury.
8.Analysis of microsatellite instability at BAT-26 locus in esophageal cancer
Kun YAN ; Mingrong WANG ; Dianchun FANG ; Kang YANG ;
Journal of Third Military Medical University 2003;0(23):-
Objective To investigate the microsatellite instability(MSI) and monomorphism at microsatellite DNA BAT 26 locus in esophageal mucosa. Methods Genomic DNA extracted from tissues was amplified by PCR. PCR products were run on 9% denaturing polyacrylamidegel and stained with silver. Then MSI and monomorphism of microsatellite at BAT 26 locus in normal tissues at the incised edge and esophageal cancer tissues in 45 cases of esophageal caner were analyzed. Results All normal specimens showed no change in the length of MS DNA at BAT 26 locus. MSI was detected in 3 out of 45(6 7%) cases of esophageal cancer. Conclusion BAT 26 has complete monomorphism in genome of normal esophageal tissues, but is not sensitive to the identification of MSI.
9.Study of the instability of microsatellite DNA and its relationship with clinical pathological parameters in esophageal cancer
Kun YAN ; Mingrong WANG ; Dianchun FANG ; Kang YANG ;
Journal of Third Military Medical University 2003;0(17):-
Objective To detect the state of microsatellite instability (MSI) and investigate the relationship between MSI and clinical pathological parameters in esophageal cancer. Methods MSI was analyzed by PCR SSCP method. Results MSI was detected (22 22%) in 45 cases of esophageal cancer. Effects of MSI on clinical stage, pathological classification, lymph node metastasis, and invasion were not found. Conclusion MSI may be an early molecular pathway and play a certain role in the development of the esophageal cancer.
10.Effect of Jiawei Xiaoyaosan on monoamine neurotransmitter in patients with psychological stress insomnia
Biyun XU ; Yan LI ; Fang XIAO ; Biyang OU ; Zhimin YANG
China Journal of Traditional Chinese Medicine and Pharmacy 2005;0(06):-
0.05).Conclusions:The integrated medicine can regulate monoamine neurotransmitter to some extent.