2.Protective effects of total extract of astragalus(TEA) onprimary rats hepatocytes injured by CCl4 or H2O2
Yan YANG ; Min-Zhu CHEN ;
Chinese Journal of Clinical Pharmacology and Therapeutics 1999;0(04):-
Aim To study the protective effect of total extract of astragalus(TEA) on primary rat hepatocytes injured by CCl4 or H2O2 and its mechanism.Methods The primary hepatocytes were isolated by Ⅳ collaganase and injured by CCl4 or H2O2.The contents of malondialdehyde(MDA) and glutathion(GSH),glutathion peroxidase(GSHpx) activity of hepatocytes and the AST and /or ALT level in cultural supernatant were determined by general methods. Results (1) The elevation of MDA content of hepatocytes and AST level in supernatant of cultural hepatocytes,and the loss of GSH content and GSHpx activity induced by CCl4 were restored remarkably by TEA(5~80 mg?L-1);(2)The elevation of ALT level in the supernatant of hepatocytes and MDA content of hepatocytes, and the loss of GSH content and GSHpx activity induced by H2O2 were improved significantly by TEA(5~80 mg?L-1) treatment.Conclusion The results suggest that TEA possess direct protective action on primary hepatocyte in vitro injured by CCl4 or H2O2. These might be associated with its anti-oxidative activity.
3.Study on Blood Lead Level Related High-risk Factors by Answer Tree in Uigur and Han Children in Urumqi
Yan CHEN ; Jiangxia ZHAO ; Yan ZHU
Journal of Environment and Health 2007;0(07):-
Objective To explore the blood level lead and the related high-risk factors in Uigur and Han children in Urumqi by answer tree. Methods Cluster sampling was used to investigate blood lead level of 988 Uigur and Han children aged 6-10 years,the related high-risk factors were investigated by using a standardized questionnaire,the related high-risk of blood lead level was analyzed by answer tree. Results The geometric mean of blood lead concentration of children was 54.46 ?g/L,in 23.28% of the children,the blood lead concentration was more than 100 ?g/L . No significant blood lead level difference was seen between Uigur and Han children. Such high-risk factors as parents occupation,bad habits and housing condition were associated with the blood lead level. Conclusion The blood lead level and lead poisoning rate of children in Urumqi are higher,many factors may influence the blood lead level of children in Urumqi.
4.Autologous leukocyte′s labeling for the diagnosis of patients with suspected intraabdominal infection
Xinguo ZHANG ; Xiaodan ZHU ; Yan CHEN
Chinese Journal of General Surgery 1997;0(06):-
Objective To evaluate the effectiveness of scanning with labeled autologous leukocytes for the detection of abdominal inflammation in equivocal patients. Methods From July 1998 to April 2000 sixteen patients with equivocal abdominal inflammation were recruited into this study. ResultsThere were 10 cases with positive scanning. The diagnosis of intraabdominal infection was consequently confirned by laparotomy and or good response to antibiotic therapy.The 6 cases with negative result were eventualy proven to have no intraabdominal infection. The average radioactivity of imaging and paired non imaging district was determined ( P
5.Quality Analysis of Cortex Moudan from Different Parts of Anhui Province
Qingjun ZHU ; Yan CHEN ; Wenxiu SUN
Chinese Journal of Information on Traditional Chinese Medicine 2006;0(06):-
Objective To analyze the quality of Cortex Moudan which grew for four years from main planting parts in Anhui province.Methods According to Pharmacopoeias of People Republic of China(2005 edition),the water content,the total ash content,ash content of acid infusibility,the content of the thing soak out and paeonol content were measured to investigate the quality of Cortex Moudan.Results Paeonol content of Cortex Moudan collected from main planting parts in Anhui province was from 0.98% to 1.77%.Conclusion The quality of Cortex Moudan from Nanling is the best of all.
6.Study on the beneficial effect of dual peroxisome proliferator-activated receptor agonist WY14643 on vascular endothelium
Chen QU ; Liang TANG ; Yan ZHU
Journal of Chinese Physician 2012;14(6):746-749
ObjectiveThe present study aimed to determine whether or not dual paroxysm proliferator-activated receptor (PPAR) agonist,WY14643,improved the dysfunctioned vascular endothelium in hypertension by reducing endothelium-derived contracting factors ( EDCFs ),and to explore the molecular mechanism it was involved in.MethodsIsometric tension in isolated thoracic aortic rings of spontaneously hypertensive rats was recorded.Endothelium-dependent contractions evoked by acetylcholine in the presence of L NAME were reduced by fenofibrate.Cyclooxygenase 1 ( COX1 ) activities were determined by analyzing the peroxidase activity of cyclooxygenase colorimetrically by using ELISA kit.ResultsCompared to the control group,WY14643 significantly decreased the vasoconstriction in aorta of the SHR rats(P=0.014).PPARα antagonist MK866 enhanced the vascular contractility of SHR rats that were incubated with 10.0μmol/L WY14643( P=0.021 ).PPARΥ antagonist GW9662 did not significantly affect the vascular contractility of SHR rats that were incubated with 10.0 μmol/L WY14643( P=0.061 ).The levels of serum PGFlα(P=0.012),2α( P =0.019) and TXB2(P=0.023) in SHR rats incubated with 10.0 μmol/L WY14643 were significantly lower than the control group,respectively.Under the condition of the existence of vascular endothelium,the expression of COX-1 in SHR rats incubated with WY14643 was significantly lower than that in SHR rats incubated without WY14643 (P=0.017).ConclusionsThose data showed that WY14643 reduced the release of EDCFs,it suggests that WY14643 protects against vascular diseases through the PPAR activators in spontaneous hypertension.
7.Paroxysmal hemicrania:clinical analysis of 8 cases
Kaiyun ZHU ; Yan HUANG ; Hui CHEN
Chinese Journal of Neurology 2008;41(6):397-399
Objective To clinically analyze the feature of paroxysmal hemicrania in order to improve our cognition toward it.Methods Eight patients,3 men,5 women,aging 17 to 74 years old,were prospectively analyzed over the past 2 years in our hospital.Results Their age of onset was from 9 to 60years old(mean 42.5±16.3).Seven of the 8 cases were treated with indomethacin,out of whom 5 got an immediate and complete response and one of them remitted partially.Another stopped taking indomethacin because of gastroenteric side effects.She was treated with verapamil and prednisone and partial relief was gained.Conclusions Paroxysmal hemicranial is a rare benign disorder.which needs our improved understanding.The patient who is diagnosed with paroxysmal hemicranial should firsfly receive indomethacin.and standard anti-cluster headache medications or other non-steroid anti-inflammatory drugs is used if she/he can not get relief and (or)tolerate the adverse effects.
8.Application and Security Observation of Acupuncture in Labor Analgesia
Yan ZHAO ; Cuiping ZHU ; Xiaohong CHEN
Shanghai Journal of Acupuncture and Moxibustion 2017;36(8):905-909
Objective To observe the therapeutic efficacy and safety of acupuncture and epidural anesthesia during full-term labor. Method A total of 199 eligible subjects experiencing natural labor were randomized into four groups. The acupuncture group was intervened by acupuncture at acupoints; the epidural group was given epidural anesthesia;the combined analgesia group was given acupuncture at acupoints plus epidural anesthesia; the control group was not given any interventions for analgesia. The Visual Analogue Scale (VAS) was adopted to record and compare labor pain (cervix dilation 3 cm at 0 min, 30 min, and 60 min, and at full cervix dilation); meanwhile, the active period of the first stage of labor, cervix dilation speed, the second stage of labor, Caesarean section (C-section) rate, instrumental delivery rate, Apgar score, and post-labor bleeding amount (2 h) were also recorded. The contents of blood β-endorphin (β-EP) and 5-hydroxytryptamine (5-HT) were monitored during the first stage of labor. Result Compared with the control group, the VAS scores dropped in the other three groups, and the decreases were similar in the epidural group and combined analgesia group, while the decrease in the acupuncture group was the least significant. Regarding the labor-related indexes, the cervix dilation speed was higher in the acupuncture group than in the control group(P<0.05), while there was no significant difference between the acupuncture group and control group in comparing the duration of the second labor stage(P>0.05). There were no significant between-group differencesin comparing the post-labor bleeding amount and Apgar score of the new-born babies(P>0.05), but compared with the epidural group, there was no use of assisted delivery. The content of blood β-EP during the first stage of labor increased in the acupuncture group and combined analgesic group(P<0.05), but there were no significant changes in the epidural group and control group(P>0.05). Conclusion Acupuncture analgesia can reduce labor pain and shorten labor duration without affecting the second stage of labor and security-related indexes.
9.Clone of apoptosis-related genes in prostate cancer cell by means of an improved subtractive hybridization
Chen SHAO ; Feng ZHU ; Wei YAN
Chinese Journal of Urology 2001;0(08):-
Objective To set up an apoptosis model of prostate cancer cell line and to clone and study the apoptosis related genes. Methods An apoptosis model of prostate cancer cell line DU-145 has been set up through induction by all transretinoic acid (ATRA).During the process of cell apoptosis the apoptosis-related gene was cloned by means of improved PCR-based subtractive hybridization from the apoptosis prostate cancer cell line DU-145 prostate cancer cells. Results During the process of DU-145 cell apoptosis,c-erb B-2 expression,TNF genes and some unknown apoptosis-related gene were observed.This has been accepted by Genebank,the accession number being AF174394. Conclusions ATRA-induced apoptosis of DU-145 cells is a complex process with multiple genes involved,some of which being unknown yet.
10.Updates on microRNA in body fluids.
Chinese Journal of Pathology 2010;39(6):424-427