Adult ; Ear Canal ; pathology ; Ear Neoplasms ; Female ; Humans ; Neurofibromatoses

Aim To study the protective effect of total extract of astragalus(TEA) on primary rat hepatocytes injured by CCl4 or H2O2 and its mechanism.Methods The primary hepatocytes were isolated by Ⅳ collaganase and injured by CCl4 or H2O2.The contents of malondialdehyde(MDA) and glutathion(GSH),glutathion peroxidase(GSHpx) activity of hepatocytes and the AST and /or ALT level in cultural supernatant were determined by general methods. Results (1) The elevation of MDA content of hepatocytes and AST level in supernatant of cultural hepatocytes,and the loss of GSH content and GSHpx activity induced by CCl4 were restored remarkably by TEA(5～80 mg?L-1);(2)The elevation of ALT level in the supernatant of hepatocytes and MDA content of hepatocytes, and the loss of GSH content and GSHpx activity induced by H2O2 were improved significantly by TEA(5～80 mg?L-1) treatment.Conclusion The results suggest that TEA possess direct protective action on primary hepatocyte in vitro injured by CCl4 or H2O2. These might be associated with its anti-oxidative activity.

Objective To explore the blood level lead and the related high-risk factors in Uigur and Han children in Urumqi by answer tree. Methods Cluster sampling was used to investigate blood lead level of 988 Uigur and Han children aged 6-10 years,the related high-risk factors were investigated by using a standardized questionnaire,the related high-risk of blood lead level was analyzed by answer tree. Results The geometric mean of blood lead concentration of children was 54.46 ?g/L,in 23.28% of the children,the blood lead concentration was more than 100 ?g/L . No significant blood lead level difference was seen between Uigur and Han children. Such high-risk factors as parents occupation,bad habits and housing condition were associated with the blood lead level. Conclusion The blood lead level and lead poisoning rate of children in Urumqi are higher,many factors may influence the blood lead level of children in Urumqi.

ObjectiveThe present study aimed to determine whether or not dual paroxysm proliferator-activated receptor (PPAR) agonist,WY14643,improved the dysfunctioned vascular endothelium in hypertension by reducing endothelium-derived contracting factors ( EDCFs ),and to explore the molecular mechanism it was involved in.MethodsIsometric tension in isolated thoracic aortic rings of spontaneously hypertensive rats was recorded.Endothelium-dependent contractions evoked by acetylcholine in the presence of L NAME were reduced by fenofibrate.Cyclooxygenase 1 ( COX1 ) activities were determined by analyzing the peroxidase activity of cyclooxygenase colorimetrically by using ELISA kit.ResultsCompared to the control group,WY14643 significantly decreased the vasoconstriction in aorta of the SHR rats(P=0.014).PPARα antagonist MK866 enhanced the vascular contractility of SHR rats that were incubated with 10.0μmol/L WY14643( P=0.021 ).PPARΥ antagonist GW9662 did not significantly affect the vascular contractility of SHR rats that were incubated with 10.0 μmol/L WY14643( P=0.061 ).The levels of serum PGFlα(P=0.012),2α( P =0.019) and TXB2(P=0.023) in SHR rats incubated with 10.0 μmol/L WY14643 were significantly lower than the control group,respectively.Under the condition of the existence of vascular endothelium,the expression of COX-1 in SHR rats incubated with WY14643 was significantly lower than that in SHR rats incubated without WY14643 (P=0.017).ConclusionsThose data showed that WY14643 reduced the release of EDCFs,it suggests that WY14643 protects against vascular diseases through the PPAR activators in spontaneous hypertension.

Objectives To investigate fluoride in drinking waters and fluorosis status and evaluate the effectiveness of fluoride-reducing projects in Shaanxi Province from 2005 to 2007. Methods In the Shaanxi province-wide, the 10 endemic areas of fluorosis were chosen according to historical data as focusing areas for investigation. The village was considered as investigation spot, 5 water samples were collected from each village for investigating of fluoride content. Four water samples were collected from each fluoride-reducing project for evaluating its effectiveness. Fluoride concentrations in drinking water were measured by fluoride-selective electrode method or speetrophotometry. When fluoride content in drinking water was greater than 1.00 mg/L, the epidemical study wasd conducted to investigate fluorosis patients, focusing on investigating of dental fluorosis prevalence in 8 to 12-year-old children and skeletal fluorosis prevalence in adults. Dental fluorosis of children was diagnosed by using Dean's method, and adult skeletal fluorosis was diagnosed according to National Standard for Clinical Diagnosis of Endemic Skeletal Fiuorosis(GB 16396-1996). Results The fluoride content in drinking water from 6390 villages was measured. The fluoride content of drinking water of 2619 villages ranged from 1.0 to<2.0 mg/L, where 1 654 998 people exposed. Additionally, the fluoride content of water of 845 villages ranged from 2.0 to<4.0 mg/L, where 355 623 people exposed. Moreover, the fluoride contents of water of 272 villages exceeded 4.0 mg/L, where 111 466 people exposed. The median of fluoride content in drinking water was 1.15% in the whole province, and fluoride content in drinking water exceeded 1.00 mg/L in Weinan, Xianyang and Yulin where were concentrated distribution areas of high fluoride water. Among 3115 fluoride-reducing projects, the fluoride content of drinking water of 1269 projects ranged from 1.0 to<2.0 mg/L, where 1 415 877 people exposed. Additionally, the fluoride content of drinking water of 120 projects ranged from 2.0 to<4.0 mg/L, where 43 888 people exposed. Moreover, the fluoride content of drinking water of 14 projects exceeded 4.0 mg/L, where 5960 people exposed. The detectable rate of dental fluorosis of 8 to 12 year-old children and skeletal fluorosis of adults reached 37.4%(16 489/44 081) and 5.1%(15 877/310 993), respectively. Conclusions The widely distribution of high-fluoride in drinking water still contributes to the prevalence of fluorosis in Shaanxi Province. The quality of fluoride-reducing projects should be further improved.

Objective To observe the therapeutic efficacy and safety of acupuncture and epidural anesthesia during full-term labor. Method A total of 199 eligible subjects experiencing natural labor were randomized into four groups. The acupuncture group was intervened by acupuncture at acupoints; the epidural group was given epidural anesthesia;the combined analgesia group was given acupuncture at acupoints plus epidural anesthesia; the control group was not given any interventions for analgesia. The Visual Analogue Scale (VAS) was adopted to record and compare labor pain (cervix dilation 3 cm at 0 min, 30 min, and 60 min, and at full cervix dilation); meanwhile, the active period of the first stage of labor, cervix dilation speed, the second stage of labor, Caesarean section (C-section) rate, instrumental delivery rate, Apgar score, and post-labor bleeding amount (2 h) were also recorded. The contents of blood β-endorphin (β-EP) and 5-hydroxytryptamine (5-HT) were monitored during the first stage of labor. Result Compared with the control group, the VAS scores dropped in the other three groups, and the decreases were similar in the epidural group and combined analgesia group, while the decrease in the acupuncture group was the least significant. Regarding the labor-related indexes, the cervix dilation speed was higher in the acupuncture group than in the control group(P<0.05), while there was no significant difference between the acupuncture group and control group in comparing the duration of the second labor stage(P>0.05). There were no significant between-group differencesin comparing the post-labor bleeding amount and Apgar score of the new-born babies(P>0.05), but compared with the epidural group, there was no use of assisted delivery. The content of blood β-EP during the first stage of labor increased in the acupuncture group and combined analgesic group(P<0.05), but there were no significant changes in the epidural group and control group(P>0.05). Conclusion Acupuncture analgesia can reduce labor pain and shorten labor duration without affecting the second stage of labor and security-related indexes.

Objective To analyze the quality of Cortex Moudan which grew for four years from main planting parts in Anhui province.Methods According to Pharmacopoeias of People Republic of China(2005 edition),the water content,the total ash content,ash content of acid infusibility,the content of the thing soak out and paeonol content were measured to investigate the quality of Cortex Moudan.Results Paeonol content of Cortex Moudan collected from main planting parts in Anhui province was from 0.98% to 1.77%.Conclusion The quality of Cortex Moudan from Nanling is the best of all.

Objective Agaro-oligosaccharides have been proved to possess the antioxidative ability in vitro. This work focus on the preparation of agaro-oligosaccharides and their antioxidant effect in vivo. Method Agaro-oligosaccharides were hydrolytically obtained and the activated carbon column was used to purify the oligosaccharides. The antioxidative effects of the 10 % ethanol eluted fraction on tissue peroxidative damage induced by carbon tetrachloride (CCl 4) was also investigated in rat. Result Agarobiose of high purity was consequently eluted from chromatography in the fractions of 5 % and 8 % ethanol, agarotetraose and agarohexaose in 10 % to 15 % ethanol, as well as high degree of polymerization (DP) oligosaccharides in 25 % ethanol. The antioxidative results indicated that agaro-oligosaccharides could elevate the activities of SOD, GSH-Px and decrease the level of MDA, GPT, GOT significantly. At a dose of 400 mg?kg~ -1, MDA level was reduced 44 % and 21 % in liver and heart, the activities of SOD and GSH-Px would amount to the peak value in liver and serum, while GPT level was decreased 22 % in serum. Conclusion The purity of agaro-oligosaccharides with different range of DPs was improved by activated carbon column, which possesses the property of different absorbance ability towards oligosaccharides with various DPs and high isolating capability. The animal test also indicated that agaro-oligosaccharides could inhibit the oxidative damage in vivo.

Objective To evaluate the effectiveness of scanning with labeled autologous leukocytes for the detection of abdominal inflammation in equivocal patients. Methods From July 1998 to April 2000 sixteen patients with equivocal abdominal inflammation were recruited into this study. ResultsThere were 10 cases with positive scanning. The diagnosis of intraabdominal infection was consequently confirned by laparotomy and or good response to antibiotic therapy.The 6 cases with negative result were eventualy proven to have no intraabdominal infection. The average radioactivity of imaging and paired non imaging district was determined ( P

Objective To evaluate the effectiveness of multiple quantitative fluorescence PCR ( QF-PCR) as a rapid technique for prenatal diagnosis of common chromosome aneuploidies , in order to optimize the prenatal diagnosis and shorten the period of diagnosis.Methods Totally 731 amniotic fluid samples of pregnant subjects ,who were referred to the Women′s Hospital School of Medicine Zhejiang University during August 2013 and September 2015, were analyzed with conventional karyotype and the QF-PCR technique by short tandem repeat(STR) markers to detect chromosomes 13,18,21,X and Y aneuploidies.There were 558 samples detected by single blind method , 173 samples detected by double blind method.Results All of the 731 amniotic fluid samples were tested in this study by QF-PCR and the results were compared to the conventional cytogenetic analysis results of the same sample.Totally 558 samples with single blind method detected 5 trisomy 21, 2 trisomy 18, 1 trisomy 13, 1(45,X), 1(47,XXY), 1(47,XYY), 1(47,XXX) and 1(69,XXX), 173 samples with double blind method detected 1 trisomy 21 and 1 trisomy 18.The rapid QF-PCR assay was successful to detect all aneuploidies involving chromosomes 21, 18, 13, X and Y in prenatal diagnosis , which were verified by chromosome karyotype analysis.The results of QF-PCR method were compared with the results of chromosome karyotype analysis , the positive rate was 15/16, the negative rate was 100%(715/715).Non chimeric chromosome abnormality detection rate was 15/15.Conclusions The multiple QF-PCR was a reliable method of detecting common chromosome aneuploidies for rapid prenatal diagnosis.As an important supplement of karyotype analysis , it was of great significance to optimize and improve the prenatal diagnosis system , and might provide more appropriate diagnostic methods for pregnant women.