2.Clinicopathological study of deep angiomyxoma
Yan ZHAO ; Hong YU ; Wei XIAO ; Bo SUN
Cancer Research and Clinic 2009;21(7):447-449
Objective To study the clinicopathologic features and differential diagnosis of deep angiomyxoma(DAM). Methods Seven cases of DAM were collected from 2000 to 2008. All the patients were examined by microscopy and immunohistochemistry. Results 6 patients with DAM were females and 1 was male, with median age of 48.5 years. Median maximum dimension was 5.9cm, with invasive growth pattern. The tumor cells of DAM were infantile, spindle or stellate,diffuse and nodular arrangement. A distinctive histologic feature of DAM was its vascularity. Non-arborizing, thin-wall, ectastic capillaries or more commonly, small thick-wall vessels were dispersed throughout the tumor. Mast cells and extravasated red blood cells were frequently found in the stroma, immunohistochemical study showed that 7 cases were positive for vimentin, desmin, ER and PR, 5 for CD34 and SMA, and negative for S-100 and CK. Conclusion DAM is a rare soft tumor that occurs principally in the vulval and vagina region of woman. Misdiagnosis has happened frequently. Immunohistochemical staining are helpful to diagnosis for DAM, but no significance to distinguish it.
3.Report of a case of sphenoid sinus meningoencephaloceles misdiagnosed as cysts.
Yan-hong XU ; Ding-qing HUANG ; Hai-bo YANG
Chinese Journal of Otorhinolaryngology Head and Neck Surgery 2010;45(5):430-430
Diagnostic Errors
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Encephalocele
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diagnosis
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Female
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Humans
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Meningocele
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diagnosis
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Middle Aged
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Mucocele
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diagnosis
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Sphenoid Sinus
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pathology
4.Pharmaceutical research progress of rhynchophylla based on chemical stability.
Bo HAO ; Xiu-Juan YANG ; Yi FENG ; Yan-Long HONG
China Journal of Chinese Materia Medica 2014;39(23):4532-4537
Rhynchophylla is a Chinese herb commonly used in clinical practice. It's also the primary herb of some famous Chinese herbal compound such as Tianma Gouteng decoction, and Lingyang Gouteng decoction. According the record from many previous materia medica literatures, rhynchophylla should be added later during decoction. Pharmaceutical research showed that rhynchophylla alkaloids were not stable. Which has resulted in many problems in the research and its application. For example, there was not a quantitative determination method in "Chinese Pharmacopoeia" of past and present versions, which seriously impacted its quality control and product application. Firstly, records from previous materia medica literatures and "Chinese Pharmacopoeia" were systematically sorted based on the chemical stability of rhynchophylla. Secondly, pharmaceutical research including chemical compositions and their stability, pharmacological effects, extraction process and quality analysis, was reviewed after reference of literatures published at home and abroad in recent decades. Positive reference and evidence for further research and development of rhynchophylla will be provided in the article.
Animals
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Drug Stability
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Drugs, Chinese Herbal
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chemistry
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pharmacology
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Humans
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Quality Control
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Uncaria
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chemistry
5.Human bone marrow derived mesenchymal stem cells regulate Th17 cells of peripheral blood in vitro
Simei REN ; Hong LU ; Ying YAN ; Haifei WANG ; Bo XIE
Journal of Leukemia & Lymphoma 2013;22(7):406-409
Objective To investigate the in vitro effects of human bone marrow derived mesenchymal stem cells (hBMSC) on Th17 cells of the human peripheral blood.Methods The density gradient centrifugation combined with lymphocyte separation medium was used to isolate hBMSC,which were then cultured.Cytokine IL-17 in the peripheral blood from a healthy person was measured by enzyme-linked immunosorbent assay (ELISA).Proportion of Th17 cells was evaluated by flow cytometry.Results The expression level of IL-17 in spent culture supernatant of the healthy person PBMC and AML hBMSC was (292.32±37.25) pg/ml,and was significantly higher than that of the healthy person PBMC and healthy hBMSC [(169.64±17.47) pg/ml,P < 0.01].There was no significant difference between the expression level of IL-17 in spent culture supernatant of the healthy person PBMC and ALL hBMSC [(159.89±23.71) pg/ml] and the level of the healthy person PBMC and hBMSC.The percentages of Th17 cells of co-culture systems from hBMSC,ALL-hBMSC,and AML-hBMSC and PBMC were (10.13±2.19) %,(13.77±4.04) % and (21.53±5.05) %,respectively,with the result between AML patients and healthy people being statistically different (P < 0.01).ALL patients and healthy people showed no difference (P > 0.05).Conclusion AML-hBMSC promotes the CD~ T cells to generate Th17 cells,which suggests that the MSC from AML marrow may play a role in the regulation of immune suppression.
6.Effects of estrogen on the expression of MMP-2, TIMP-2 and TGF-β1 in human corneal stromal cells
Yan, TIAN ; Hong-Bo, YIN ; Ying-Ping, DENG
International Eye Science 2017;17(7):1232-1236
AIM:To investigate the effects of estrogen on the expression of matrix metalloproteinases-2(MMP-2), tissue inhibitor of metalloproteinases-2(TIMP-2) and transforming growth factor-β1(TGF-β1) in cultured human corneal stromal cells.METHODS: Inflammatory environments of human corneal stromal cells were simulated by using 1.5ng/mL IL-1β.The cells were then treated with or without different concentrations of estrogen(0, 1×10-4, 1×10-6, 1×10-8, 1×10-10mol/L estradiol)in vitro.Cell viability was evaluated by MTT.Expression levels of MMP-2, TIMP-2 and TGF-β1 proteins were measured by enzyme-linked immunosorbent assay(ELISA).RESULTS:Estrogen did not affect the viability of human corneal stromal cells.Compared with the control group, expression levels of MMP-2 and TGF-β1 proteins in E2 treatment group significantly decreased after being treated with estrogen, while the expression level of TIMP-2 significantly increased.CONCLUSION: Estrogen could, to some extent, down-regulate the expression of MMP-2 and TGF-β1 and up-regulate the expression of TIMP-2, which might contribute to protecting human cornea.
7.To Strengthen Information Analysis and Evaluation Competency——On the Education of Information Literacy of Medical Students
Hong HU ; Wenlong ZHAO ; Chunli LI ; Bo LV ; Yan HE
Chinese Journal of Medical Education Research 2005;0(05):-
As society develops,it has become one of the important goals of higher medical personnel training to cultivate medical students' information literacy.After explaining the definition of information literacy,this paper discussed the importance of information analysis and evaluation in information literacy education.
9.Inhibitory Effect of Hydroxyapatite Particles with Different Size on Malignant Melanoma A375 Cells: A Preliminary Study.
Bo GUO ; Bo LI ; Yan WANG ; Youliang HONG ; Lingli ZHANG ; Xingdong ZHANG
Journal of Biomedical Engineering 2015;32(4):832-837
To explore the inhibitory effect of hydroxyapatite (HA) particles with different sizes on malignant melanoma A375 cells in vitro, we synthesized 4 short rod-like HA particles using TIPS. Their mean diameters were 998.0 nm (HA1), 511.0 nm (HA2), 244.0 nm (HA3), and 71.6 nm (HA4), respectively. Malignant melanoma A375 cells were co-cultured with HA particles in vitro. Results showed that HA particles smaller than 511.0 nm in mean diameter could always inhibit proliferation of A375 cells, and nanometer-HA particles (HA4) had the strongest inhibitory effect on A375 cell proliferation and the strongest inducing effect on apoptosis. HA particles were distributed in plasma of A375 cells. The ultrastructure changes of A375 cells were found most significant in nanometer-HA particles (HA4) group. We conclude that particle size is a very important influencing factor on anti-tumor effects of HA and that nanometer-HA particle has the strongest inhibitory effect on tumor cell proliferation.
Apoptosis
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Cell Line, Tumor
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Cell Proliferation
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Durapatite
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chemistry
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Humans
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Melanoma
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Nanotubes
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chemistry
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Particle Size
10.Studies on methods of in vitro evaluation of soft rot-resistance in jinxianlian.
Qing-Song SHAO ; Hong-Bo LIU ; Jie GUO ; Yan XU ; Run-Huai HU ; Ming-Yan LI
China Journal of Chinese Materia Medica 2014;39(1):44-47
Compared with living spray method, it focused on the investigation of different inoculation methods, various inoculation concentration and the influence of different seeding age on soft rot-resistance in Jinxianlian. The results showed that (1) Inoculated with dropping connection, the difference of disease index between A. roxburghii and A. formosanus was grate, so that the disease-resistance could be obviously distinguished. (2) When the inoculation concentration was 1.0 x 10(7) cfu x mL(-1), the difference of disease index was relatively obvious and the disease-resistance could be differentiated well. (3) At the moment of 4-month seeding inoculation, a certain difference of the disease index between A. roxburghii and A. formosanus was existed, so, relatively, it could accurately reflect the resistance difference between various species. With the inoculation of dropping connection, A. roxburghii and A. formosanus of 4-month seeding age was put in the bacteria suspension of inoculation concentration of 1.0 x 10(7) cfu x mL(-1). The identification was taken up after 5 days in the incubator under the condition of 14 h daylight and 28 degrees C. The identification result was conformed with that of the living spray method. To investigate the identification method of in vitro evaluation of soft rot-resistance of Jinxianlian so as to provide the foundation for germplasm utilization and excellent cultivars breeding.
Plant Diseases
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microbiology