Objective To evaluate the immunological effect of neonatal hepatitis B vaccine and to prevent the immunization effect of low and no responders. Methods A total of 1,200 infants were selected from Yantai City, Shandong Province. All the infants were immunized for the first time after birth, and were inoculated with brewer's yeast hepatitis B vaccine 5μg. The venous blood of the infant was taken to separate the serum and the geometric mean concentration of anti-HBs in serum by using chemiluminescent microparticle immunoassay. Of which 128 cases were low, no response were immunized again: 71 cases were treated with brewer's yeast hepatitis B vaccine 5μg and 57 cases were inoculated with 10%Hansenula hepatitis B vaccine again and again, three times. The results of the re-immune were evaluated . Results There were 80 cases (62.5%) wtith normal response, 23 cases (17.9%) with high response, 22 cases (17.2%) with low response, 3 cases (2.3%) with no response The immunization response rate of HBsAg positive in fathers and mothers was significantly lower than that in county, city and above, and the fathers' mother HBsAg was negative. There was no significant difference in the positive rate of antibody transfection between the first and the third of the above two yeasts, but the geometric mean concentration of anti-HBs was significantly lower for both vaccines, and the brewer's yeast vaccine was significantly lower. Conclusion The immune response rate of hepatitis B vaccine in children with HBsAg-negative mothers at the county, municipal and above hospitals is relatively high. Compared with the brewer's yeast vaccine, the effect of the hepatitis B vaccine is obviously better .

In TCM prevention and treatment of acute coronary syndrome(ACS),attention should be paid attention to the following:(1)modern pharmacology of TCM and pathology of unstable plaque;(2)microcosmic and objective indexes of TCM treatment based on syndrome differentiation for interfering unstable plaque of ACS;(3)treatment of ACS based on the theory of collateral diseases;(4)treatment of ACS based on the theory of toxicity;(5)treatment of ACS based on the theory of blood stasis;(6)clinical by-stage of pathological state of coronary atherosclerosis plaque and treatment based on syndrome differentiation.

? AlM: To observe the differences of central cornea thickness, anterior chamber depth, corneal anterior curvature and corneal posterior curvature between the Kazak and the Han nationality college emmetropia students, and analyze the relationship of corneal thickness and corneal curvature.?METHODS: More than 500 students in grade one in Xinjiang Medical University for screening, selected 55 emmetropia eyes in Han nationality students and 51 in Kazak students. Sirius corneal topography was applied to the measurement.? RESULTS: Kazak and the Han nationality college emmetropia students' central cornea thickness ( Kazak:0. 52± 0. 03mm, Han:0. 54 ± 0. 03mm), anterior chamber depth (Kazak:2. 97 ± 0. 31mm, Han: 3. 14 ± 0. 25mm) had significant difference (P<0. 05). Corneal anterior curvature and corneal posterior curvature were no significant difference (P>0. 05). Han had no significant relationship in corneal thickness and corneal anterior curvature (r<0), or in corneal posterior curvature ( r < 0. 1 ) . Kazak had no significant relationship in corneal thickness and corneal anterior curvature (r<0), or in corneal posterior curvature(r<0. 1).? CONCLUSlON: There are significant differences between the Kazak and the Han nationality college emmetropia students in cornea thickness, anterior chamber depth. There is no significant relationship between corneal thickness and corneal curvature (K1, K2) in Kazak and the Han nationality college emmetropia students.

Objective To establish content determination of hydroxyl safflower yellow A in Biyangqing.Methods Hhgh-performance liquid chromatography(HPLC)was used in the determination.A C18 column was used for the separation flow rate Was set at 1.0mL/min,the temperature of the column was set at 30℃,and wavelength of diction was set at 403 nm.with 100.08%average recovery and 0.98%RSD.Conclusion This detrmination method is specific and reproducible and can be used to control the quality of Biyangqing.

Objective To compare the clinical value of nerve stimulator-versus ultrasound-guided continuous femoral nerve block for analgesia after laparoscopic surgery.Methods Forty patients,aged 18-60 yr,with body mass index of 18-30 kg/m2,of ASA physical status Ⅰ or Ⅱ,scheduled for elective laparoscopic surgery,were randomly assigned into 2 groups (n =20 each) using a random number table:nerve stimulator group (group S) and ultrasound group (group U).Epidural anesthesia was performed with 1.73 ％ carbonated lidocaine in both groups.0.2％ ropivacaine 5 ml/h was infused continuously after surgery to perform femoral nerve block for analgesia.VAS score at rest was assessed at 2,6,24 and 48 h after surgery.At 24 and 48 h after surgery,VAS scores during active and passive movement were assessed.The time for catheter placement near the femoral nerve and development of subcutaneous hematoma at the puncture site,local anesthetic intoxication and nausea and vomiting were recorded.The postoperative requirement for analgesics was also recorded.Results There was no significant difference in the VAS scores and puncture for femoral nerve block-and local anesthetics-related adverse events between the two groups.The time for catheter placement near the femoral nerve was 8.0 ± 1.4 and (6.7 ± 0.9) min in S and U groups,respectively,and the time was significantly longer in group S than in group U.No patients required rescue analgesic after surgery in both groups.Conclusion Nerve stimulator-guided continuous femoral nerve block provides higher clinical value than ultrasound-guided continuous femoral nerve block for analgesia after laparoscopic surgery and it is more suitable for clinical application.

ObjectivesTo assess the cardiovascular function in obese children.MethodsA total of 30 obese children and 50 age- and sex-matched normal weight children were enrolled and treadmill test was performed. Exercise tolerance, the time to target heart rate and continuous T-wave alternans (TWA) during treadmill test were compared between two groups. ResultsCompared with normal weight children, the exercise tolerance was decreased and the time to target heart rate was shortened, while the incidence of continuous TWA was increased in obese children and there was signiifcant difference between two groups (P<0.01).ConclusionsObesity may lead to cardiovascular dysfunction in childhood which should be concerned.

Objective To examine the relationship between plasma soluble interleukin 2 receptor(sIL2R), bone metabolism markers, and bone mineral densities in female patients with Graves′ disease(GD), and to explore the role of sIL2R in bone metabolism of GD.Methods This study included 201 female GD patients.FT3, FT4, TSH, PTH, osteocalcin(OCN), C-terminal telopeptides of type Ⅰ collagen (CTX), procollagen type I N-terminal propeptide(PINP), and 25-(OH)-vitamin D(25OHD) were measured using electrochemical luminescence technique.sIL2R was measured using chemiluminescence method.All pations underwent examinations of bone mineral densities at lumbar spine, total hip, and femoral neck(LS-BMD, TH-BMD, and FN-BMD).The associations of sIL2R with bone metabolism markers and bone mineral densities were analyzed.Results OCN,CTX,PINP gradually decreased across the tertile groups of LS-BMD, TH-BMD, FN-BMD(all P<0.05).FT3, sIL2R gradually increased across the tertile groups of OCN, CTX, PINP(all P<0.05).Multiple linear regression analysis showed that sIL2R was independently associated with OCN and CTX(both P<0.05).Conclusion The increase of bone turnover leads to bone loss in female GD patients.The plasma level of sIL2R was positively correlated to bone turnover, which indicated that sIL2R may play an important role in bone metabolism of GD.

Objective To understand the typing and clinical significance of M-protein,to raise the understanding and diagnostic level through analysis of 639 patients with M-protein.Methods To analysis age,sex and disease of M-protein positive patients,charateristic and content difference of multiple myeloma(MM)and monoclonal gammopathy of undeterminal significance(MGUS).Results In 639 patients with M-proteinemia,there were 409 patients with IgG-type(64%),80 IgA-type(12.5%),79 IgM-type(12.4%),4 IgD-type(0.6%),9 ? light chain(1.4%),18 ? light chain(2.8%),27 double clone(4.2%),oligoclone(2.0%).There were 115 MM(18.0%),13 Waldenstrm's macroglobulinemia(2.0%),1 primary amyloidosis of kidney,19 non-Hodgkin's lymphoma(NHL)(3%),5 chronic lymphocytic leukemia(CLL)(0.8%),473 MGUS(74.0%).Conclusion M-proteinemia is a clinical phonomenon,mainly in MM and lymphocytic proliferative disease.There is an improving tendency in MGUS detectable rate.

0.05). ④ High correlations were also found between 2-DE, RT-3DE derived LVEDV and TV (r= 0.80- 0.88), but 2-DE and RT-3DE 2-plane methods underestimated TV significantly (P

Purpose:To observe the effect of thalidomide combined with chemotherapy in the treatment of newly diagnosed multiple myeloma. Methods:34 patients were divided into two groups at random. 14 cases in the treated group were initially administered thalidomide at doses ranging from 100mg~200 mg/d,and the dosage was escalated by 100mg~200 mg every two weeks until patients could not tolerate the treatment.The maximal dose did not exceed 600mg/d.MP or M 2 protocol was delivered to those patients in the treated group concomitantly.20 cases in the control group received chemotherapy consisting of MP or M 2 protocol only.Evaluation of 3-months effective(partial remission plus progress)rate,one-year event free survival and two-years overall survival was done in both groups. Results:3-months effective rate is 85.7% in the treated group and 60% in control group.One-year EFS is 71.4% in the treated group while that in the control group is 35%.Two-years OS are 57.1% and 25% respectively.The side effects of thalidomide are tolerable,and constipation is the most common. Conclusions:The combination of thalidomide and chemotherapy is an effective treatment for newly diagnosed MM,and it demonstrates an improvement in effective rate,one-year EFS and two-years OS.