Objective To investigate the effect of penehyclidine (PHCD) pretreatment on Toll-like receptor 4 (TLR4) mRNA expression in the lung following acute lung injury (ALI) induced by hemorrhagic shock in rats. Methods Forty healthy SD rats of both sexes weighing 200-250 g were randomly divided into 5 groups( n = 8 each): group Ⅰ sham operation (group S); group Ⅱ ALI (group ALI); group Ⅲ, Ⅴ, PHCD 0.3,1.0, 3.0 mg/kg were given iv respectively at 30 min before hemorrhagic shock (P1-3). Hemorrhagic shock was induced by exsanguinations. MAP was maintained at 35-45 mm Hg for 60 min. The animals were killed at 4 h after resuscitation. Their lungs were removed for microscopic examination, W/D lung weight ratio and determination of TLR4 mRNA expression in the lung tissue (by RT-PCR). NF-κB p65 protein expression in the lung tissue was determined (by immuno-histochemical staining). Results Hemorrhagic shock significantly increased TLR4 mRNA and NF-κB p65 protein expression in the lung tissue and W/D lung weight ratio. Pretreatment with PHCD 1.0 or 3.0 mg/kg significantly inhibited hemorrhagic shock-induced increase in TLR4 mRNA and NF-κB p65 protein expression in the lung and W/D lung weight ratio. The lung injury was significantly ameliorated in group P2,3 as compared to group ALI. Conclusion PHCD pretreatment can attenuate ALI induced by hemorrhagic shock through down-regulation of TLR4 mRNA expression and decreasing NF-κB activity in the lung.

Objective To observe the effect of Tangluotong Decoction(Decoctions of removing obstruction of meridians for treating diabetes)based on the principle of reinforcing kidney,activating blood and removing obstruction of meridians on the early diabetic nephropathy(DN)patients.Methods Totally 68 DN patients were randomized into two groups.In addition to the routine treatment for diabetes,the treatment group(38 patients)was administered Tangluotong Decoction while the control group(30 patients)was giv- en Lossartan Potassium Tablets.The clinical symptoms and signs and lab indices before and after treatment were observed.Results The comprehensive total effective rate of the treatment group was 89.47% while that of control group was 60.00%,the difference was significant(P

Medicine, Chinese Traditional

Objective To compare the effect of three types of lipid emulsions on the IL-1βand IL-6 expressions in acute lung injury induced by lipopolysaccharide (LPS) in rats. Methods One hundred young SD rats were divided into control,LPS,ω-6,ω-9 andω-3 group. Age 29 days, the control group were intravenously injected with saline,ω-6 group injected lipid emu-sion (C14-24),ω-9 group injected long chain fat emusion,ω-3 group injected fish oil fat emusion. Then the control group fol-lowed by intratracheal instillation of saline, and the reamining four groups were sprayed with LPS. Pathologic changes in lung tissue section were observed. The expression levels of IL-1βmRNA and IL-6 mRNA were determined by RT-PCR,and the con-centrations of IL-1βand IL-6 in bronchoalveolar lavage fluid (BALF) were measured by enzyme-linked immune-specific assay. Results Infiltration and bleeding were observed in lung tissue under light microscopy in ALI rats. The lung indexes and patho-logical scores of ALI model groups were significantly higher than those of control group (P<0.005). The expressions of IL-1βand IL-6 mRNA in groupsω-9 andω-3 were significantly higher than those in groups LPS andω-6 (P<0.005). The levels of IL-1βand IL-6 in BALF in groupsω-9 andω-3 were significantly higher than those in groups LPS andω-6 (P<0.005). There was no significant difference (P>0.005) of the expressions of IL-1βand IL-6 mRNA betweenω-9 andω-3 group. Conclusions ω-6 PUFAs can accentuate inflammation by up-regulating the levels of IL-1βand IL-6 whileω-9 PUFAs andω-3 PUFAs can relieve inflammation by down-regulating the levels of IL-1βand IL-6.

Objective To evaluate the clinical efficacy and safety of traditional Chinese medicine on internal and external treatment of acne.Methods 128 patients in Dermatology Department of Xiyuan Hospital CACMS from May 2012 to March 2013 were collected and randomly divided into Chinese medicine group and western medicine group.The patients of Chinese medicine group were divided into invasion of the Lung by Wind-Heat syndrome and damp-heat oflmg and stomach syndrome,respectively taking acne liquid 1 or 2 orally and using the herb mask once a week.The western medicine group took Roxithromycin Capsules orally and smeared 0.3％ Viaminate and Vitamin E Cream.The treatment period lasted for 6 weeks,and collected the photograph of the target lesion before and after research every 2 week and evaluated the efficacy.Result The total efficacy rate were 90.77％ (59/65) and 74.60％ (47/63) for Chinese medicine group and western medicine group respectively,with significant difference between them(x2=11.83,P＜0.05).The efficacy rates were 87.50％ and 93.94％ for invasion of the Lung by Wind-Heat syndrome and damp-heat of lung and stomach syndrome respectively,with no significant difference between them(x2=0.416,P＞0.05).The TCM symptom scores after treatment [Chinese medicine group (4.36 ± 2.45),western medicine group (8.62± 2.49)] had significant difference in two groups respectively compared with before treatment[Chinese medicine group(15.33±6.08),western medicine group(14.34±5.29),P＜0.05].The difference of TCM symptom scores after treatment between the two groups also had a significant difference (P＜0.05).The TCM symptom scores after treatment of invasion of the Lung by Wind-Heat syndrome group and damp-heat of lung and stomach syndrome group were (4.50±3.01) and (4.24±1.83),which had a significant difference compared with before treatment respectively[(15.37±7.03)and(15.29±5.13),P＜0.05].Conclusion The TCM internal and external treatment on acne could reduce the number of lesions and improve TCM symptom scores.The effect of treating invasion of the Lung by Wind-Heat syndrome and damp-heat of lung and stomach syndrome with TCM was almost equal.

Objective To observe the dynamic changes of TNF-α,IL-1β,IL-6,IL-10,and expressions of Notch-1,NF-κB mRNA and their protein levels in the brain tissue of septic mice and intervention effects of intrathecal injection of lentiviral vector of miR-34a gene for regulating Notch-1/NF-κB signaling pathway.Methods A total of 54 mice of clean grade were divided randomly (random number) into four groups,namely sham group (n =9),in which sham-operated laparotomy was performed;CLP group (n=15),in which the cecum ligation operation (CLP) was performed;NC group (n =15),in which intrathecal injection of lentivirus 5 μL (concentration 5 × 108 TU/mL),one time per day,for 3 days,then CLP was performed on the seventh day;intervention group (n =15),in wihch intrathecal injection of miR-34a lentivirus 5 μL (concentration 5 × 108 TU/mL),one time per day,for 3 days,then CLP was performed on the seventh day.The mice of four groups were sacrificed 24 h after modeling or operation.The changes of behavior of mice was observed and the neurological scores were assessed 24 h after CLP.The levels of TNF-α,IL-1β,IL-6 and IL-10 in the brain were measured by ELISA method.The mRNA expression and protein levels of Notch-1 and NF-κB in the brain tissue were measured by real-time PCR and Western blot method,respectively.Pathological changes of brain tissue were observed under light microscope.Results The neurological scores,the cerebral TNF-α,IL-6 levels,the mRNA expression and protein level of NF-κB (P＜0.01),and IL-1β levels (P ＜0.05) in CLP group 24 h after modeling were higher than those in sham group.The cerebral IL-10 level and the mRNA expression and protein level of Notch-1 (P ＜ 0.01) in CLP group 24 h after modeling were lower than those in sham group.The neurological scores,the cerebral TNF-α and IL-1β levels,and the protein level of NF-κB (P ＜0.01),and IL-6 (P ＜ 0.05) in NC group 24 h after modeling were higher than those in sham group.The cerebral IL-10 level,the mRNA expression and protein level of Notch1 in NC group 24 h after modeling were lower than those in sham group.There were no significant difference in neurological socres,IL-1β and IL-6 levels between intervention group and CLP group (P ＞ 0.05).The IL-10 level (P ＜ 0.05) and the mRNA expression (P ＜ 0.05) and protein level (P ＜ 0.01) of Notch-1 in intervention group 24 h after modeling were lower than those in CLP group.There were no significant difference in biomarkers between NC group and CLP group (P ＞ 0.05).The degree of cerebral damage found under light microscope was also ameliorated in intervention group compared with CLP group 24 h after modeling.Conclusions The effects of miR-34a via regulating Notch-1/NF-κB signaling pathway on brain function exerts cerebral protective effects in septic mice.

The paper described the necessity of the doctor-patient information exchange platform,and defects found in existing platforms. Such a platform is conducive to higher hospital informationization, better patient satisfaction, and better social and economic benefits of the hospital.The hospital may improve its quality of care and patient satisfaction by means of the platform

The transesterification reaction conditions of lard with methyl acetate with combined use of immobilized lipases as catalysts were conducted. Initially, according to single factorial experiments, the studies on Lipozyme TL IM and Novozym 435 respectively catalyzed transesterification of lard showed that the optimal parameters of transesterification reaction were: the molar ratio of methyl acetate to oil of 14∶1, 40% enzyme added based on oil weight, temperature 50℃. Combined use of Lipozyme TL IM and Novozym 435 was proposed further to improve the catalytic performance by the response surface method (RSM). Herein, a 5-level-3-factor central composite rotated design was employed to evaluate the effects of lipase loading, the proportion of the two lipases and amount of methyl acetate. The optimum conditions were as followings: 40% lipase loading based on oil weight, 50%/50% the proportion of lipases (Novozym 435/Lipozyme TL IM), and the molar ratio of methyl acetate to oil of 14∶1. And under the optimal conditions, the highest biodiesel yield of 97.6% could be attained, which was higher than the biodiesel yield with each single one of the two lipases. The results suggested that the technics of combined use of certain immobilized lipases catalyzed transesterification reaction of lard for biodiesel production with methyl acetate as the acyl acceptor could raise the FAME yield and save the production cost.

A bacterium that contains a chitin deacetylase has been isolated from ocean soil. It has been identified Baciluus .The best suitable condition of producing this enzyme is that pH is 4.0,35℃,and the incubate time is 80 hours in the present of Ca 2+ as well as having no chitosan. The best temperature of this enzyme when it acts is 40℃～50℃ and the best pH range is 4.5～5.0.

Objective To explore the risk factors for inguinal hernia in children.Methods A 1:1 matched case-control study which based on hospital group data was carried out.201 cases aged 0～6 years with inguinal hernia and controls were enrolled,and their parents were investigated with questionaire.Conditional logistic regression model was used for single factor and multivariate factoranalysistoestimateoddsratios(OR) and the 95% eonfidence interval(95% CI).Results Inguinal hernia was related to ehildren ' s constipation(0R=3.520,95%CI:1.238~10.015),children's cry(OR=6.532,95%CI:2.651~16.091),mother ate pickles(OR=2.529, 95%CI:1.271~5.031) and maternal anemia(OR=6.809,95%CI:2.530～18.322) during the period of one month before pregnancy and the first 3 months of gestation,the children's family history of inguinal hernia(OR=I 1.250,95%CI:4.766～26.553).Conclusion Children's constipation,children's cry,maternal anemia and eating pickles during the period of one month before pregnancy and the first 3 months of gestation,children ' s family history of inguinal hernia are the risk factors for children' s inguinal hernia.