Objective To present the CT and MRI findings of the fibrous dysplasia(FD) of the spine.Methods CT and MRI findings were retrospectively evaluated in 19 cases of FD of spine,all of which were confirmed pathologically.Nineteen patients underwent CT plain scanning(19/19) and 5 had enhanced CT scanning(5/19).Patients were also examined by MRI plain scan(11/11) or MRI enhanced scan (6/11).The location of tumors,the type of bone destruction,the boundary of lesions,internal intensity or signal,the enhancement pattern of lesions,and presence of compression fractures,spinal deformity were observed.Result Nine cases had monostotic FD,while 10 had polyostotic FD.In all the 49 lesions of 19 cases,13 lesions were located in the cervical vertebrae,23 lesions in the thoracic vertebrae,11 lesions in lumbar vertebrae,and 2 lesions in sacral vertebrae.Thirty-three lesions involved both vertebral body and appendix.Pure osteolysis were found in 26 lesions on CT examinations.Peripheral osteosclerosis rims (41/49) and expansive lesions(32/49) were seen.Residual bone crest(28/49) and ground-glass opacity(23/49)were noted.Different degrees of vertebral compression were found in 19 lesions.Five patients had spinal deformity.On T1WI,14 lesions showed intermediate or low signal,and 10 lesions presented as heterogeneous signal.On T2WI,6 lesions had low signal intensity,4 lesions were noted as hyperintensive,and 14 lesions presented as heterogeneous signal.Multiple fluid-fluid levels were found in 1 lesion.Low signal rims were seen in 14 lesions.Twenty lesions of 11 patients had significant enhancement.Conclusion Expansive pattern,ground-glass opacity,peripheral osteosclerosis rims and significant enhancement were helpful findings for the diagnosis of spinal FD.

Objective To investigate various sex hormones and the their relation in patients with polycystic ovary syndrome (PCOS).Methods 40 patients with PCOS were matched with 40 age matched healthy women,study group was divided into the obese group and non-obese groups based on body mass index,insulin resistance and non-insulin-resistance based on insulin sensitivity index. The level of follicle stimulating hormone (FSH), luteinizing hormone (LH), prolactin (PRL), estradiol (E2), testosterone (T), androstene dione (A4), sulfal-dehydroepiandrosterone (DHEAS), sex hormone-binding globulin (SHBG), fasting glucose(FG), fasting insulin (FIN) was measured in both group. Homeostasis model assessment insulin resistance (HOMA-IR), insulin sensitivity index (ISI) and free androgen index (FAI) were caulculated. Results ①In the PCOS group,LH, LH/FSH, T, A4, FAI, FIN and Homa-IR were significantly higher compared to the control,while FSH, SHBG, ISI were significantly lower (P<0.05),PRL, FG, E2, DHEAS level did not show difference (P>0.05). ②There were significant differences in T, FAI, A4, but not in DHEAS between the PCOS group and the control one. ③There were significant differences between the hirsute group and the non-hirsute group in T, FAI, A4 (P<0.05). ④In the obese PCOS group compared to the non-obese PCOS group, T, A4, DHEAS were not significantly different, but FAI and Homa-IR were significantly higher, SHBG, ISI, LH/FSH were significantly lower. ⑤The quantity of insulin resistance in the increased T group was significantly higher compared to the common T group. ⑥In the insulin resistance group compared to the non insulin resistance group, there were not significant differences of A4、DHEAS,T and FAI was significantly higher.⑦In PCOS group,there were significant positive correlation between FAI useful parameter compared to T, A4, DHEAS for the diagnosis of PCOS. Obese PCOS women have more severe and BMI,FAI and Homa-IR;BMI and LH/FSH were significant inverse correlation. Conclusions FAI is more endocrine secretion and metabolic disturbance than non PCOS women. Hyperandrogenism and insulin resistance are consanguineous correlation.

Objective To isolate and culture meshenchymal stem cells from murine fetal liver.Methods flMSCs from mouse fetuses were isolated by adhering to plastic surface.Growth kinetics was determined by growth curve.Cell cycle and phenotype were analyzed by FACSan flow cytometry.Differentiation of adhering cells was induced and identified.Results Homogenous fibroblast-like cells were predominated in culture.The counting of flMSCs increased 2 fold after 24 hours and 83.76%?2.88% of flMSCs were in G0/G1 phases.flMSCs were CD44,CD29 positive but negative for the markers of hematopoietic cells such as CD45,CD11b.flMSCs were able to differentiate along adipogenic,chondrogenic and osteogenic pathways even after being passaged several times.ConclusionflMSCs can be isolated by their plastic-attachable property and can expand without losing their multiple differentiation potential in vitro.flMSCs may offer an appropriate cell source for stem cell therapy.

ObjectiveIn order to confirm the relationship of pathogenic factors and clinical manifestation of cerebral palsy. Methods185 children were divided into the prematurity group(91 cases) and maturity group(94 cases). The μ-test was applied to analyze the incidence of clinical manifestation of 185 children with cerebral palsy for different pathogenic factors. ResultsThe sever symptoms occurred more frequently in prematurity group than in maturity group. ConclusionThe earlier the careful follow-up for children with prematurity was performed, the earlier the diagnosis and treatment of cerebral palsy were achiened.

BACKGROUND: Reproductive activity of hepatocytes is limited. There are numerous studies concerning stem cells differentiation into hepatocytes, including embryonic stem cells, bone marrow cells, pancreas stem cells, neural stem cells, various sources of mesenchymal stem cells.OBJECTIVE: To explore the possibility of induction of mesenchymal stem cells (MSCs) from umbilical cord blood (UCB) into hepatocyte-like cells in vitro culture.DESIGN, TIME AND SETTING: A cytological in vitro study was performed at the Institute of Hematology, Medical College of Jinan University from October 2005 to April 2006.MATERIALS: Fetus cord blood was obtained from spontaneous delivery and caesarean delivered healthy pregnant women at the Department of Obstetrics and Gynecology, First Affiliated Hospital of Jinan University. The parturients signed the informed consents.METHODS: UCB-MSCs were incubated in vitro, and digested in trypsin-EDTA. The third passage of cells at 5 × 10~4 cells/cm~2 wereinoculated. Original medium was removed 48 hours later. Cells were washed in phosphate-buffered saline. In the first phase, cells were incubated in F12 medium supplemented with dexamethasone, hepatocyte growth factor, epidermal growth factor and 1 ×its for 2 weeks. In the second phase, cells were incubated in F12 medium containing dexamethasone, hepatocyte growth factor,oncostatin M and 1 × ITS for two weeks.MAIN OUTCOME MEASURES: The following parameters were measured: expression of surface marker of UCB-MSCs using flow cytometry, expression of related gene and protein of hepatocytes following induction respectively using RT-PCR and immunofluorescence staining.RESULTS: No CD34/CD45/CD14 of hematopoietic markers were detected, either no the CD54, CD49f, HLA-DR were found. The low expression of CD106 and high expression of CD29, CD44, CD13 were found. The gene expression of a-fetoprotein, albumin,CK-18 and TAT were discovered, and three kind of protein a-fetoprotein, albumin, CK-18 were positively observed in cytoplasm after 4 weeks of induction using immunofluorescence staining.CONCLUSION: UCB-MSCs are able to differentiate into hepatocyte-like cells in vitro culture following combination with many growth differentiation factors, such as dexamethasone, hepatocyte growth factor, epidermal growth factor, tumorigenesis M and ITS.

AIM: To explore the possibility of differentiating functional insulin-producing cells from human BM-derived stem cells. METHODS: Mesenchymal stem cells were isolated from human bone marrow. Then these cells were induced with epidermal growth factor, β-mercaptoethanol and high concentration of glucose. The gene expression related to islet β cells was detected by RT-PCR. Insulin in the treated cells was examined by immunocytochemistry. In addition, the levels of insulin secretion and glucose-stimulated insulin release were examined by microparticle enzyme immunoassay. Finally, the induced cells were implanted into the right renal subcapsular space of diabetic mice. Blood glucose levels were monitored 16 d after implantation. The right kidneys of the treated mice were harvested for immunohistochemistry. RESULTS: The key genes related to pancreatic β cells had been confirmed to express by PCR and insulin was detected by immunocytochemistry in differentiated human BM-derived stem cells induced by high glucose, which responded to glucose challenge. Furthermore, implantation of the cells in renal subcapsular space was able to lower the glucose levels in hyperglycemic mice. After 16 days, the implanted cells were determined still to be insulin positive cells by immunohistochemistry. CONCLUSION: These results indicate human BM-derived stem cells are capable of differentiating into functional insulin-producing cells and may represent a pool of cells for the treatment of diabetes.

Objective To investigate the effects of controlled hypotension induced with sodium nitroprusside (SNP) or nicardipine on hemodynamics during hip operation and postoperative hepatic and renal function.Methods Twenty ASA Ⅰ or Ⅱ patients aged 20-70 yrs weighing 50-80 kg scheduled for total hip replacement or open reduction and internal fixation of fracture of acetabulum were randomized to receive either SNP (group Ⅰ ) or nicardipine (group Ⅱ ) for induced hypotension during operation. Each group included 10 patients. Radial artery and right internal jugular vein were cannulated for BP and CVP monitoring. The probe of Hemo-Sonic ?100 esophageal supersonic hemodynamic monitor (Arrow U.S.A) was placed in the esophagus and fixed when best signal was obtained. ECG, hemodynamics including MAP, HR, CVP, CO and other parameters, SpO2 and PetCO2 were continuously monitored. Anesthesia was induced with propofol 2 mg?kg-1 , fentanyl 2-3 ?g? kg-1 and vecuronium 0.1 mg?kg-1 and maintained with 1.5% isoflurane and continuous infusion of propofol ( at 4-6 mg?kg-1 ?h-1 ) and intermittent i.v. boluses of vecuronium after tracheal intubation. MAP was reduced to 55-65 mm Hg with SNP (at a rate of 0.5-8.0 ?g?kg-1?min-1 ) or nicardipine (at 1.0-8.0 ?g?kg-1 ?min-1 ) . Intraoperative blood loss and blood transfusion and postoperative drainage from the wound were measured and recorded.Hemodynamic parameters were recorded before hypotension and 15, 30 and 60 min after start of induced hypotension and 15 and 30 min after recovery from hypotension. Hepatic and renal functions were measured before and after operation using total bilirubin, AST, ALT, BUN and creatinine. Results The two groups were comparable with respect to sex ratio (M/F) , age, height, body weight, duration of induced hypotension and operation. MAP and systemic vascular resistance (SVR) were significantly reduced and tachycardia developed during hypotension in both groups. The left ventricular ejection time was significantly prolonged and aortic blood flow (ABF) and cardiac output (CO) were significantly increased and CVP was lowered during hypotension as compared to the baseline values in SNP group. BUN was significantly reduced after operation ( P

OBJECTIVE:To promote the improvement of the quality of pharmaceutical care.METHODS:Patients’com_ plaints in2003～2004in the pharmacy department of our hospital were analyzed and the related information was considered in the formulation of countermeasures.RESULTS&CONCLUSIONS:Giving consideration of patients'complaints that served as information resources in pharmaceutical management can promote a steady improvement of the quality of pharmaceutical care.

Objective:To explore the methods for drug therapy regimen formulation and pharmaceutical service of clinical pharma-cists for the infection induced by peripherally inserted central catheter ( PICC) complicated with deep venous thrombosis in cancer pa-tients. Methods:Referring to the related guide, clinical pharmacists put forward concrete opinions on how to choose anti-infective drugs and anti-thrombotic drugs for a cancer patient with PICC infection complicated with deep venous thrombosis. Clinical pharmacists sug-gested that vancomycin be used for the infection and low molecular weight heparin sodium for prophylactic anticoagulation. Meanwhile, pharmaceutical care for blood clotting function should be strengthened. Results:The proposals of clinical pharmacist were partly adopt-ed by clinicians. After the therapy, the temperature of the patient returned to normal, and the deep venous thrombosis was well con-trolled. The patient was out of hospital smoothly. Conclusion:Through the participation in clinical practice, clinical pharmacists can assist physicians in optimizing treatment plan and summarize the pharmaceutical care mode for the PICC infection and deep venous thrombosis in cancer patients, which can provide instructions for pharmaceutical care in the future.

Objective In this study, we try to understand the effects of microenvironment on the differentiation of mesenchymal stem cells (MSCs) by coculturing MSCs with mature cardiomyocytes or culturing MSCs in cardiomyocyte-lysate, in this study. Methods MSCs isolated from mature rats were either cocultured with cardiomyocytes isolated from new born rats with the ratio of 1 to 4, or cultured in the medium containing 4-fold cardiomyocyte-lysate obtained by repeated freezing and defrosting of rat myocardial cells. The morphology of MSCs under light microscopy were observed daily for 7 days and immunostaining against cTnT and CD31 was performed on the 7~ th day. MSCs cultured in ordinary medium were observed as the control. Results Both MSCs cocultured with cardiomyocytes and cultured in cardiomyocyte-lysate were differentiated into myogenic cells and expressed cTnT and CD31 at the 7th day of cultivation. The MSCs in the control group did not change in morphology and express cTnT or CD31. Conclusion Both myocardial cell coculturing system and cardiomyocyte-lysate system can be used to induce bone marrow MSCs to differentiate into cardiomyocyte-like cells and endotheliocyte-like cells.