Objective To investigate the cytocompatibility of the liching liquids of Co-Cr alloy and Ti alloy on human gingival fibroblasts (HGF). Methods The HGF were treated in vitro with leaching liquids of Co-Cr alloy and Ti alloy, respectively. The DMEM cell medium containing 10% fetal bovine serum was served as a negative control. The viability of HGF treated by two dental alloys were evaluated by means of MTT, and the contents of intracellular reduced glutathione (rGSH) were assayed by kits. The tumor necrosis factor-α(TNF-α) contents were determined in the culture supernatant by ELISA in two groups. The effects of these alloys on the expression of caspase-3 were examined by real time-PCR method. Results Compared with the control group, HGF treated with Co-Cr alloy leaching liquids showed a lower viability ( P ＜0.05), while Ti alloy leaching liquid promoted the proliferation of HGF. In Co-Cr alloy group, the rGSH content was significantly decreased (P＜0.05), while TNF-α content was significantly increased (P＜0.05) compared with control group. There were no significant differences in rGSH and TNF-α contents between the Ti alloy group and control group (P＞0.05). The expression of caspase-3 was significantly higher in Co-Cr alloy group than that of control group (P＜0.05), while there was no significant difference in the expression of caspase-3 between Ti alloy group and control group. Conclusion Results suggest that Co-Cr alloy possesses cytotoxicity, while there is better cell compatibility for Ti alloy.

The bystander effect(BE) plays an important role in the gene therapy of cancer by the herpes thymidine kinase/ganciclovir(HSV-TK/GCV) system.It enhances the therapeutic efficacy of this system.Up to now,the exact underlying mechanism of the bystander effect remains unclear.A large body of evidence has indicated a close correlation of the connexin expression and gap junction in the targeted cells to the bystander effect.Here the publications concerning the relationship of gap junction with the bystander effect in the HSV-TK/GCV treatment have been reviewed.The possible cell death signals that can be transferred through gap junction to induce the bystander effect are also discussed.

Objective To evaluate influence of benzo(a)pyrene on cell cycle of the human embryonic lung fibroblast at different synchronous stage. Methods Cell cycle distribution were detected by flow cytometry(FCM). Cells were synchronized at cycle G0 stage through 48 hours serum starvation and reenter cell cycle together at a synchronous state after resupplied with whole growth medium contain good serum. Benzo(a)pyrene with a series concentrations were directly used or metabolized active by traditional and modified metabolize active methods through preincubation of S9 and were treated as regent to HELF cells mostly synchronized at G1, S or G2-M stage respectively. Then cell cycle distribution changes were detected 12 h after treatment. Results Serum starvation(48 hours) could synchronize HELF at G0-G1 stage effectively and 10-12 h, 16-18 h, 22-24 h were cycle phase change distinctly time after serum restimulated. Cells were synchronized at G1, S and G2-M stage mostly. Benzo(a)pyrene influence cycle distribution little without metabolize active, while modified methods could metabolize active benzo(a)pyrene well and avoided disturbing effect of S9 on cell cycle in traditional method. Except 2 ?mol/L benzo(a)pyrene treated at 22 h after restimulated caused the percentage of cells at S stage increase, most treatment induced the percentage of cells at S stage decrease which was associated obviously with the increasing dosages. The percentage of cell at S stage decrease at 16 h were more remarkable than other times and the percentage of cell at G2-M increase correspondingly. While the percentage of cell at G1 increase and the percentage of cell at G2-M decrease were obsered when benzo(a)pyrene treated at 10 h and 22 h after restimulated. Conclusion Serum starvation 48 hours and restimulate can synchronize HELF at different stage effectively. Modified metabolize active method is suitable for cell cycle research. Primary influence of benzo(a)pyrene on cell cycle is the decreased percentage of cells at S stage, G1 arrest, G2-M arrest and G1 arrest were occurred when benzo(a)pyrene were treated at G1, S and G2 stage respectively.

In order to probe into the mechanism of low-dose chemotherapeutic agents in the treatment of leukemia, we studied the effects of low concentrations (l-40ng/ml) of daunorubicin (DNR) on proliferation and differentiation of human promyelocytic leukemia cell line (HL-60). 1 ng/ml DNR could inhibit the proliferation of the cells, but no induction of differentiation was found. DNR at more than 5 ng/ml had both cytotoxicity and induction of differentiation. Through the count of survival cells, [3H]TdR and [3H]UR incorporation, cloning efficiency, cell morphology and NBT reduction test, it is concluded that low-dose DNR could have inhibitory and cytotoxic effects on the cells, and its inducing differentiation was not important.

The effects of 1, 25 (OH)2D3 in combination with low-dose chemotherapeutant on the human promyelocytic leukemic cells (HL-60) were investigated. In 4-day-cultured cells, 1, 25 (OH)2D3 and low-dose harringtonine (HH) or daunorubicine (DNR) had synergic effect in inducing differentiation of HL-60 cells, and HH or DNR significantly potentiated the effect of 1, 25 (OH)2D3 in inhibiting the cell proliferation, which were demonstrated by NBT reduction test and [3H]-TdR incorporation, respectively. Cell cycle analysis by flow cytometery revealed that the cells in S phase decreased remarkably and most cells stopped at G0/G1 phase. The results provide a new way for the inducers in treatment of leukemia.

Objective To investigate the significance of detecting the level of CRP,TNF-α,IL-6 and IGF-1 in the patients with type 2 diabetes mellitus(T2DM).Methods Sixty patients with T2DM in the Pingliang Municipal People′s Hospital from February 2013 to March 2015 were selected as the observation group And contemporaneous 60 individuals undergoing healthy physical exami-nation were selected as the control group.The levels of FBG,2 hBG,FINS,HbA1c,CRP,TNF-α,IL-6 and IGF-1 were detected in these two groups.The correlation between CRP,TNF-α,IL-6 and IGF-1 with FBG,FINS and HbA1c were analyzed.Results The levels of FBG,2 hBG and HbA1c in the observation group were significantly higher than those in the control group,the difference was statistically significant (P<0.05).The FINS level in the observation group was significantly lower than that in the control group,the difference has statistically significant (P<0.05).The levels of CRP,TNF-α,IL-6 and IGF-1 in the observation group were (8.46±2.45)mg/L,(18.56±4.23)ng/L,(13.53±3.24)pg/L and (125.33±23.14)ng/L respectively,which were signifi-cantly higher than (3.23±1.02)mg/L,(11.25±3.01)ng/L,(7.24±1.78)pg/L and (98.45±18.56)ng/L in the control group, the difference was statistically significant(P<0.05).CRP,TNF-αand IL-6 were positively correlated with FBG,FINS and HbA1c;IGF-1 was negatively correlated with FBG and HbA1c.Conclusion Detecting the CRP,TNF-α,IL-6 and IGF-1 levels can provide a basis for the diagnosis of T2DM and thinking for its treatment.

OBJECTIVETo explore the effect of Chinese drugs for activating blood circulation and removing blood stasis (CDABCRBS) on carotid atherosclerotic plaque and long-term ischemic cerebrovascular events.

METHODSBy using open and control method, effect of 4 groups of platelet antagonists, platelet antagonists + CDABCRBS, platelet antagonists +atorvastatin, platelet antagonists +atorvastatin +CDABCRBS on carotid atherosclerotic plaque and long-term ischemic cerebrovascular events of 90 cerebral infarction patients were analyzed.

RESULTSThrough survival analysis, there was no statistical difference in the effect of the 4 interventions on the variation of carotid stenosis rates or ischemic cerebrovascular events (P > 0.05). The occurrence of ischemic cerebrovascular events could be postponed by about 4 months in those treated with platelet antagonists + CDABCRBS and platelet antagonists + atorvastatin +CDABCRBS. By multivariate Logistic analysis, age, hypertension, and clopidogrel were associated with stenosis of extracranial carotid arteries (P <0.05). Age, diabetes, aspirin, clopidogrel, CDABCRBS were correlated with cerebrovascular accidents (P < 0.05).

CONCLUSIONSWhether or not accompanied with hypertension is an influential factor for carotid stenosis, but it does not affect the occurrence of ischemic cerebrovascular events. CDABCRBS could effectively prolong the occurrence time of ischemic cerebrovascular events.

Adult ; Aged ; Brain Ischemia ; etiology ; Carotid Artery Diseases ; complications ; drug therapy ; Drugs, Chinese Herbal ; therapeutic use ; Female ; Humans ; Male ; Middle Aged ; Phytotherapy ; Stroke ; etiology

The causes of transient ischemic attack (TIA) are diverse and complex.It often attacks repeatedly and its early risk of stroke is high.Identifying etiology and pathogenesis and start the correct treatment in time are the important measures for the prevention of cerebral infarction.However,the conventional CT and MRI reveal no abnormalities in patients with TIA,and it brings difficulties to the clinical diagnosis and treatment.CT perfusion imaging can reflect abnormal cerebral blood flow perfusion in patients with TIA.CT perfusion imaging in combination with CT angiography may visually display its corresponding abnormal changes in the feeding artery and provide the valuable diagnostic information for the location of vascular stenosis,severity,plaque characteristics and collateral circulation.It has very an important significance for guiding clinicians to choose the best treatment options,improve clinic efficiency and observe efficacy.

Objective To investigate the correlation between epicardial fat thickness (EAT) and the risk factors of coronary artery disease (CAD) as well as the severity of coronary lesions with echocardiography,Methods According to the results of coronary angiography,80 patients underwent coronary angiography were divided into coronary heart disease group (CHD group,4 6 cases) and without coronary heart disease group (control group,34cases),and according to coronary artorrythe severity,CHD goup was divided into subgroups of single-vessel disease (24 cases) and multivessel disease subgroup(22 cases).Tho epioardial fat thickness on the free wall of the right ventricle was measured at end-systole from the para-sterna,long-axis vlews.The relationship between EAT and the risk factors of CAD (such as age,gender,hypercholesterolemia,hypertenston,diabetes,smoking,family CAD history) as well as the severity of coronary lesions were analyzed.Results EAT in multivessel disease CAD group [(8.85 ± 1.44)mm] was significantly higher than that in single vessel CAD group[(6.86 ± 1.09) mm] and control group [(4.58 ± 1.12) mm] (all P ＜ 0.01).EAT was significantly related with the risk factors of CAD.Although the risk factors of CAD such as age,blood glucose,blood pressure were adjusted,EAT was still independently associated with CAD (OR:5.394,95 ％ CI:1.700 ～ 17.1 12,P =0.004).Concluslon The level of EAT is significantly correlated with CAD and the severity of coronary lesions.

10.3969/j.issn.1000-8179.2013.12.007