OBJECTIVE: To develop a method for determination of the content of Dexamethasone acetate in Compound tretinoin ointment by RP-HPLC. METHODS: The separation was performed on C18 column with methanol: water (70∶30) as a mobile phase, wavelength of UV detector at 240nm. RESULTS: The linear range of Dexamethasone acetate was within 0.028 3～0.849 0?g(r=0.999 9). The average recovery of Dexamethasone acetate was 101.13%, with RSD at 1.28%. CONCLUSION: The method is simple, rapid and capable of producing good separation results. It can be applied to the quality control of Compound tretinoin ointment.

OBJECTIVE:To investigate situation and trend of proton pump inhibitors in 19 hospital of Hangzhou area and to provide reference for the research,production,sales and clinical application of this kind of drugs. METHODS:Utilization of proton pump inhibitors in 19 hospital of Hangzhou area during the period of 2004～2008 were analyzed statistically in respect of consumption sum,DDDs and DDC. RESULTS:Both consumption sum and DDDs of proton pump inhibitors increased quickly in recent five years. Amount of oral omeprazole(capsule and tablet)was the greatest among those categories and increased steadily. Amount of rabeprazole and esomeprazole as new type of proton pump inhibitors increased rapidly. CONCLUSION:Proton pump inhibitors have great potential for development. Omeprazole are widely used in the clinic as mature proton pump inhibitors.

Echocardiography, Three-Dimensional ; Heart Defects, Congenital ; diagnostic imaging ; therapy ; Humans

Electron Transport ; Electron Transport Complex III ; deficiency ; Humans ; Mitochondria ; metabolism

BACKGROUND:There are few reports about the effect of serum-free culture on the differentiation of rat adipose-derived stem cel s into vascular endothelial cel s.
OBJECTIVE:To investigate the isolation, serum-free culture of rat adipose-derived stem cel s differentiating into vascular endothelial cel s.
METHODS:The rat adipose-derived stem cel s were isolated from male Sprague-Dawley rats and expanded to the third passage by enzymatic digestion-adherent explants method. In the experimental group, rat adipose-derived stem cel s were cultured in serum-free medium for 24 hours. In the control group, rat adipose-derived stem cel s were cultured in low-glucose Dulbecco’s modified Eagle’s medium containing 10%fetal bovine serum. After that, the cel s were cultured in inducing medium for 3 weeks to differentiate into vascular endothelial cel s.
RESULTS AND CONCLUSION:The rat adipose-derived stem cel s grew as polygonal or fusiform-shaped adherent cel s when cultured in vitro, which could stably proliferate and passage. The rat adipose-derived stem cel s showed very low expression of CD31, a cel surface marker, after passages. After directional differentiations into vascular endothelial cel s, the cel s were pebble-shaped under the inverted microscope. Expression of CD31 was up-regulated, which was much higher in the experimental group than the control group. The induced cel s in the experimental group had stronger abilities than those in the control group to swal ow Dil-labeled acetylated low-density lipoprotein and form tube-like structures on the matrigel after differentiation into vascular endothelial cel s. So, rat adipose-derived stem cel s could be highly successful y induced to differentiate into vascular endothelial cel s in vitro after serum-free culture.

BACKGROUND:Titanium zirconium niobium tin al oy (Ti-Zr-NB-Sn al oy) has good tissue structure, mechanical properties, corrosion resistance and biocompatibility, which is an ideal dental material. OBJECTIVE:To explore the efficacy and safety of Ti-Zr-NB-Sn al oy in dental restoration. METHODS:A retrospective analysis was performed on clinical data of 87 cases of dental defects, including 43 males and 44 females, aged 22-41 years. Of the 87 cases, 43 cases were repaired with Ti-Zr-NB-Sn al oy as observation group, and the other 44 cases were treated with nichrome as control group. After 36 months of fol ow-up, the therapeutic efficacy, dental restoration conditions, dental prosthesis conditions and patient satisfaction were observed and compared. RESULTS AND CONCLUSION:Al the 87 patients were fol owed up for 36 months. After the fol ow-up, the total effective rate and patient satisfaction were higher in the observation group than the control group (P<0.05). In the control group, there were four cases of swol en gums, four cases of bleeding gums, four cases of al ergies, three cases of gum gray line, and six cases of mucosal pain;in the observation group, there was only one case of swol en gums, and the dental restoration was better in the observation group than the control group (P<0.05). For the dental prosthesis, there were 10 cases of dental stent damage and 20 cases of dental stent loosening in the control group;and only one case of dental stent damage and one case of dental stent loosening in the observation, and the observation group was superior to the control group in the dental prosthesis (P<0.05). Experimental findings suggest that Ti-Zr-NB-Sn al oy for repair of dental defects has good biocompatibility and security.

OBJECTIVE:To compare the licensed pharmacist system in China and the USA,and to provide reference for im-proving pharmaceutical care and promoting public safety of drug use in China. METHODS:The relevant literatures in recent 10 years were retrieved from CJFD and Wanfang database. The differences of legal status,access qualification,legal obligations and responsibilities of licensed pharmacists in the USA and China were compared,and suggestions on improving the licensed pharma-cists system in China were put forward. RESULTS & CONCLUSIONS:At present,the problems of licensed pharmacist system in China are mainly that the legal status is not high,the access threshold is low,the legal obligations and responsibilities are not clear. However,the licensed pharmacist system in the USA has been developed for a century and formed a relatively strict legal sys-tem. Model State Pharmacy Act promulgated by National Association of Boards of Pharmacy in USA has clearly defined the access qualifications,legal obligations and responsibilities of licensed pharmacists. The pharmacy acts of the states were enacted on its blueprint. In view of current problems in China,it is necessary to learn from the above experience of the USA to improve the legal status,access qualification,legal obligations and responsibilities of licensed pharmacists and the quality of pharmaceutical care.

Objective To explore the influence of recombinant human erythropoietin(rhEPO) injection about nitric oxide(NO) and nitric oxide synthase(NOS) level in the brain and endothelin(ET) level in the blood after hypoxia-ischemia brain damage(HIBD) in neonatal rats,and elucidate the possible mechanism of the neuroprotective effect of rhEPO administered to the rat pup prior to cerebral hypoxia-ischemia.Methods Seven days-old SD rats were divided into control group,hypoxia-ischemia group(HI) and EPO group.NO activity,NOS level in the brain and ET in the blood were determined at 6 h after hypoxia-ischemia.Results NO and NOS levels in the brain and ET level in the blood were increased after HIBD in neonatal rats(P

Objective To investigate and improve the methods of nasal feeding to reduce the occurrence of complication such as diarrhea and vomiting.Methods We separated 140 patients with heavy craniocerebral injury into the control group (50 patients) and the experimental group (90 patients) according to different nasal feeding methods. For the control group traditional method was used. For the experimental group infusion pump was used to control infusion rate. The incidence of complication was compared between two groups. And within experimental group, the same nutrient solutions in respect of type and volume were infused during 10 hours, 16 hours, and 18 hours respectively. The relationship between infusion rate and the occurrence of complication was observed, analyzed and compared to seek for the most suitable enteral nutrition route which incurred the least complication.Results The incidence of diarrhea and vomiting in the experimental group was lower than that of the control group. And there were no significant differences in the occurrence of complication by using infusion pump at three different infusion rates within the experimental group. Conclusions The application of infusion pump in nasal feeding can reduce the incidence of complication and infusing nutrient solution at a slow rate was not necessary.