1.Study on the Interface of Ti-porcelain Fused Under Different Nanomateria
Journal of Medical Research 2006;0(06):-
Objective This study was made to observe the effect of different surface treatment pure titanium on bond strengt of pure titanium and porcelain, and find the mechanism as basisi of clinic use.Methods Dental pure titanium(TA2) was choosen as material,before applied for porcelain the rest groups were treat as followings:the metal substrate surface was smeared with Nano-scale material(nano-SiO2,nano-gold and nano-palladium particles), compared with control group ,and find the change of bond strengt. The change of bond strengt in the Ti-porcelain interface area were observed with scanning electron microscopy (SEM) and electron probe microanalysis(EPMA) respectively.Results Before applied for porcelain, the metal substrate surface was smeared with nano-SiO2 and nano-gold particles,we acquired a positive effect to increase the bonding strength,while using nano-palladium particles has a negativee ffect. Conclusion The bonding strength was enhanced when the metal substrate surface was smeared with nano-SiO2 before applied for porcelain,so we adviced that it should be used in practice as a try.
2.Use of literature novelty assessment reports in medical researchers of Guizhou Province
Chinese Journal of Medical Library and Information Science 2015;(7):66-71
The use of literature novelty assessment reports in medical researchers of Guizhou Province and its in-fluencing factors were investigated with questionnaires combined with interview, and measures were put forward for improving the use and level of literature novelty assessment reports, and service for scientific research.
3.Clinical study on prevention of postpartum hemorrhage of cesarean section using hemabat in high risk pregnant women
Lian-Fang WU ; Yan LIU ; Yan RUAN ;
Chinese Journal of Obstetrics and Gynecology 2000;0(09):-
Objective To observe the effect and safety of hemabat(H)on prevention of postpartum hemorrhage in cesarean section and after cesarean section in high risk pregnant women.Methods Four hundred and sixty-nine pregnant women with high hemorrhagic risk factors including twin pregnancy, polyhydramnios,fetal macrosomia,placenta previa were planned cesarean section.A total of 457 pregnant women were divided into 3 groups by operation indications.There were 239 cases of fetal macrosomia,145 cases of twin pregnancy and polyhydramnios,and 73 cases of placenta previa.Three kinds of hysterotonics were used randomly in each group.Group oxytocin(O):20 U oxytocin injected into the uterine plus 20 U oxytocin intravascularly,152 women;Group oxytocin+hemabate(O+H):20 U oxytocin and 250?g hemabat injected into the uterine,192 women;group H:250 p,g hemabat,injected into the uterine,125 women.The amount of bleeding during the operation and within 2-hour after delivery were measured.The side effect of each group was observed.Results The amount of bleeding during cesarean section in group O was(445?262)ml,in group O+H(33?218)ml,and in group H(375?265)ml.There was an extremely significant difference between group O and group O+H(P
5.Fluid therapy in critically ill patients.
Chinese Journal of Surgery 2009;47(6):432-434
6.Effect of Nerve Growth Factor on Expression of Caspase-3 in Hippocampus of Rats with Seizures Induced by Kainic Acid
Journal of Applied Clinical Pediatrics 1994;0(04):-
Objective To observe the effect of nerve growth factor(NGF) on Caspase-3 expression in hippocampus of epileptic rats induced by kainic acid(KA) and explore the protective effect and mechanisms of NGF on the epileptic neurocytes.Methods Sixty healthy rats aged 21 to 30 days were randomly divided into 3 groups,each of which included 20 rats.Rats in group A were treated with 9 g?L-1 sodium,rats in group B were treated with KA,rats in group C were treated with KA and NGF.Concentration of KA was 2 g?L-1 diluted by 9 g?L-1 sodium;KA was injected into rats in group B and group C,intraperitoneally.Then state of seizure was recorded,and EEG and pathological section were performed.Five rats in each group were sacrificed by perfusion with paraform respectively at 6 hour,on the 1st day,on the 3rd day after injection of KA.Specimen was sampled and made into frozen section.Expression of Caspase-3 in hippocampus of epileptic rats was analyzed by immunohistochemistry.And microscope image measuring and analysis instrument was used to analyze image of expression.Results Symptoms of seizure appeared 30 min after injection of KA.Seizure was proved by detection of EEG and pathological section.Expressions of Caspase-3 detected by immunohistochemistry in group C decreased at every time spot were higher than those in group B,and the defferences between 2 groups had statistically significant(Pa
8.Contrast analysis of corneal flap thickness using Moria M290 and 110 microkeratome
yan, CHEN ; xin, SUN ; jing-cai, LIAN
Journal of Shanghai Jiaotong University(Medical Science) 2006;0(02):-
Objective To analyze the corneal flap thickness in laser in situ keratomileusis(LASIK) using Moria M2 microkeratome and to identify the related factors. Methods Sixty patients with LASIK were divided into two groups: M2 90 group,using the Moria M2 90 microkeratome,n=30;M2 110 group,using the Moria M2 110 microkeratome,n=30.All were performed on both eyes with the right one treated first.Subtraction pachymetry was used to measure corneal flap thickness which was analyzed statistically with the data including age,preoperative corneal diameter,curvature,corneal thickness and refraction. Results In the 30 patients of M2 90 group,the mean corneal flap thickness of right eye and left eyes were(128.03?12.03)?m(105~156 ?m) and(123.40?12.38) ?m(92~147 ?m),respectively,and the corneal flap thickness were statistically different between the right and left eyes(P
9.Study on Method of Expanding and Culturing Rat Mesenchymal Stem Cells in vitro and Its Biological Characteristics
Qin YU ; Junlan LIAN ; Yan WANG
Journal of Zhejiang Chinese Medical University 2006;0(02):-
[Objective] To establish a method to seperate rat mesenchymal stem cells(rMSCs) from rat marrow, culture rMSCs in vitro, and study its biological characteristics. [Methods] rMSCs were seperated from rat marrow with wall-sticking method and expanding cultured in vitro. To draw the growth curves of cells at 1th, 3th, 5th passage, detect the surface antigens with flow cytometry. To evaluate the effect of fetal calf serum with different concentrations to rMSCs. [Results] There were approximately (5~6)?105 cells after primary culture and over (2~3)?108 rMSCs at 5th passage. The appearance of rMSCs is like shape of spear and its growth curves are like shape of S. The rMSCs were positive for CD90 and negative for CD45. [Conclusions] Cells seperated and cultured by the method established in this experiment were rMSCs. They have the biological characteristics of rMSCs, and are good resources for study in tissue engineering.
10.Current Situation of Medical Ethics Education in Institute of Medicine and Nursing and Strategies to Improve the Effectiveness of Teaching
Xuejun HE ; Yan WU ; Lian DONG
Chinese Medical Ethics 2017;30(7):897-900
A scientific and appropriate construction of curriculum of medical ethics such as teaching hours,teaching content,teaching methods and examination & evaluation in Institute of Medicine and Nursing is to improve the effectiveness of the teaching of medical ethics,to fulfil its goal of fostering applied skilled medical talents,and to meet the needs for the talents in the development of time so that students can better access and understand medical ethics for the sake of facing and dealing properly with problems related to medical ethics work in their future medical work.