Objective To investigate the relationship between hOGG1 and XPD gene polymorphism and genetic susceptibility of gastric cancer.Methods This study was carried out in Dongying Shengli Petroleum Administration Bureau Hospital.Under the narrow band imaging(NBI) mode,the blood samples of a total of 98 gastric cancers and 80 controls without cancers were collected.Genetic polymorphisms of DNA repair genes were identified by polymerase chain reaction restriction fragment length polymorphism(PCR-RFLP),then its relationship with cancers was analyzed.Results Carrying 326Cys allele with the wine increased the risk of gastric cancer; Lys 751Gln genotype increased the susceptibility of the gastric cancers (OR =1.486,0.73 ～ 3.025).Conclusion Lys751 Gln genotype increases the risk of gastric cancer.

Objective To investigate the feasibility of application of plasma anticoagulant heparin and hepa-rin lithium instead of serum in clinical biochemistry.Methods The clinical data 50 healthy persons in our hospital were statistically analyzed.Results The Potassium (K),sodium (Na),calcium (Ca),low -density lipoprotein (LDL),lactate dehydrogenase(LDH),cytokines(CK),blood urea nitrogen(BUN),serum uric acid(UA),alkaline phosphatase(ALP),magnesium(Mg),the standard value of total cholesterol(CHO),serum total bilirubin(TBil), direct bilirubin(DBil),triglyceride(TG)and other items of anticoagulant heparin plasma were (4.42 ±0.27)mmol/L, (137.00 ±1.70)mmol/L,(2.56 ±0.41)mmol/L,(115.53 ±88.93)mmol/L,(5.06 ±1.18)mmol/L,(281.56 ± 67.36)mmol/L,(84.41 ±74.02)μmol/L,(0.99 ±0.18)U /L,(4.62 ±1.26)μg/L,(17.18 ±15.66)mmol/L, (2.22 ±2.66)μmol/L,(2.28 ±7.70)mmol/L,which were significantly lower than those in serum(t =-3.415,-2.017,-4.739,-3.571,-3.155,-2.778,-4.117,-7.65,-10.799,-5.677,-3.192,-8.625,-5.401,-3.483,all P <0.05.);The K,Na,TCa,BUN,UA,ALP,DBil,TP,Mg,CK,HDL -C,LDL,LDH and other items of lithium heparin anticoagulant plasma were (4.51 ±0.30)mmol/L,(137.06 ±1.56)mmol/L,(2.60 ±0.08)mmol/L, (5.08 ±1.22)mmol/L,(281.86 ±67.78)μmol/L,(84.83 ±74.82)U /L,(2.22 ±2.56)μmol/L,(74.15 ± 10.94)mmol/L,(0.99 ±0.17)μg/L,(118.47 ±92.77)U /L,(1.68 ±0.72)mmol/L,(2.55 ±0.66)mmol/L, (129.82 ±30.34)U /L,which were significantly lower than those in serum(t =-3.517,-3.273,-4.128,-3.101,4.749,-2.271,-4.586,-6.706,-10.095,-5.837,2.234,-7.309,-7.175,-14.875,all P <0.05.);The HDL -C,LDL,BUN,Cr,TBil,DBil,Na,Cl,UA,GGT,ALP,ALT,AST,TP,ALB,TBA,Mg,CHO,LDH, CK,CK -MB,AMS,TG,Glu 24 and other items of heparin lithium heparin anticoagulant plasma and serum had good correlation(r >0.800).Conclusion Plasma anticoagulant heparin and heparin lithium instead of serum is feasible in clinical biochemistry.

Objective:To understand the relationship between child malnutrition and social determi-nants among children under the age of 5 in China,and to provide evidence and useful information to help policy makers develop social policies to improve child nutritional status.Methods:Information of 2 434 children aged 0 -5 was extracted from year 1 991 to 201 1 longitudinal survey data in the China Health and Nutrition Survey (CHNS)was extracted for analysis.Child underweight,child stunting,and child wasting were defined using World Health Organization Child Growth Standards for weight-for-age,height-for-age,and weight-for-height.Weight-for-age values,height-for-age values or weight-for-height values below 2 standard deviations were considered as underweight,stunting and wasting.World Health Organi-zation igrowup software was used to calculate the prevalence of child underweight,child stunting,and child wasting.Multivariate Logistic regression model was used to analyze the relationship between child malnutrition and social determinants (household income,parents’educational level,living regions,and communities’urbanization level).Results:The prevalence of child underweight and child stunting were decreased by 64.8% and 67.8%,respectively from 1 991 to 201 1 ,while the prevalence of child wasting had remained at a relatively low level (below 5%).The problem of child underweight and stunting had been significantly resolved in China.Female children had better outcomes than male children on impro-ving nutritional status.Among all the non-socio-economic determinants of child malnutrition,children with low height mother and children had inadequate protein intake were both risk factors of malnutrition. The social determinants significantly associated to child malnutrition included:living in the western re-gions and central regions,living in low level urbanization communities,with low household incomes,and low maternal educational levels.Conclusion:In order to further decrease the prevalence of child malnu-trition and alleviate the inequity of child health,we should pay more attention to the social determinants behind child malnutrition.Besides,social policies beneficial to child nutrition promotion need to be taken urgently,the important social policies suggested by the researchers included focusing on maternal and child dietary intake,improving household economic situation,improving maternal educational level, and balancing economic development and resource distribution between different regions in China.

BACKGROUND:Recent studies have shown that osteoclast differentiation factor is closely related to osteoclast differentiation, formation and function in bone remodeling during orthodontic tooth movement. OBJECTIVE:To observe the effects of three kinds of orthodontic appliances on the expression of osteoclast differentiation factor at the pressure side of rat periodontal tissue during remodeling process and to explore the biocompatibility of the orthodontic appliances with the host tissues during orthodontic treatment. METHODS:Eighty healthy Wistar rats were selected to establish animal models of orthodontic tooth movement, and then randomly divided into four groups: control group, MBT group, Begg group, Damon Ⅲ appliance group. Four animal from each group were sacrificed at 3, 7, 14 days after wearing orthodontic appliances. The tartrate-resistant acid phosphatase staining was used to count the osteoclasts at the pressure side of alveolar bone tissue; real-time quantitative PCR detection to detect mRNA expression of osteoclast differentiation factor at the pressure side of periodontal tissue and time distribution characteristics. RESULTS AND CONCLUSION:Compared with the control group, the number of positive osteoclasts and mRNA expression of osteoclast differentiation factor at the pressure side of the alveolar bone tissue were increased with orthodontic time, reached the peaked at 7 days and then gradualy decreased. The number of positive osteoclastsand mRNA expression of osteoclast differentiation factor at the pressure side of the alveolar bone tissue were significantly higher in the Damon Ⅲ group than the other three groups at 7 days after orthodontic treatment (P < 0.05). These findings indicate that, during the bone remodeling, the number of positive osteoclasts changed in accordance with the mRNA expression of osteoclast differentiation factor, and at 7 days, the number of positive osteoclasts and mRNA expression of osteoclast differentiation factor were highest in the Damon Ⅲ group.

Selective serotonin reuptake inhibitors (SSRIs),a new generation of anti-depressant agent,are mainly used to treat emotional disorders in clinical practice now.Recent studies have found that SSRIs can increase neurogenesis after cerebral ischemia,promote neurovascular reconstruction,and are conducive to the recovery of neurological function.Furthermore,SSRIs can improve the tolerance of ischemic tissue,exert anti-inflammation and anti-oxidation properties,reduce the blood-brain-barrier injury,and inhibit excitatory amino acid toxicity in order to exert neuroprotective effect.This article reviews the related research progress in SSRIs and ischemic stroke.

BACKGROUND:There is no design that can completely rule out the intermittent impact damage to implants, therefore, a new bal attachment-retained implant system is constantly updated and developed. OBJECTIVE:To evaluate the clinical effectiveness of an implant-supported mandibular overdenture retained with a bal attachment. METHODS: We searched the Cochrane Library, PubMed, Medline, EM-base, WanFang Data, CNKI, VIP and other databases by computer to colect randomized controled trials addressing the implant-supported mandibular overdenture retained with a bal attachment and other control methods for dentures. The time limit was from database creation to February 2014. Two researchers independently completed literature screening according to inclusion and exclusion criteria, data extraction and quality assessment. RevMan 5.1 software was used for meta-analysis. RESULTS AND CONCLUSION: There were 10 studies included in result analysis, including 7 from China and 3 from other countries. Analysis results showed that statistical heterogeneity was remarkable in included studies, and there was no significant difference in patient’s satisfaction, clinical and objective indicators, and complications, suggesting that this approach continues to be explored in clinic. The implant-supported bal attachment-retained mandibular overdenture is relatively expensive, which is identical with the current research progress that is in the exploration stage worldwide. Due to the limited quantity and quality of included studies, the conclusions of this systematic review only provide references for clinical practice and research. The implant-supported bal attachment-retained mandibular overdenture stil needs further exploration and improvement.

Objective To discuss the importance of early di agnosis and treatment of primary hy-perparathyroidism in multiple bone resorptions.Methods From April 1997to April 2001,5patie nts were regarded as having multiple bone resorptions.There were 4females and 1male.The age ranged from 25to 46years(average 37years).The mean interval between symptom o nset and final diagnosis was 19.5mon ths(from8to 36months).All patients were suffering from se vere bone pain in need of strong antalgic,and the symptoms including fatigue,depression,loss of appetite and malaise,e tc.Abnormality on bone scan indicated multiple areas of skeletal involvem ent,bone radiographs showed multip le bone resorptions in skull,rib,pelvis and limbs.The range of serum calcium level was 2.27-3.22mmol /L and that o f PTH was 168-214ng /ml.Palpable neck mass was found i n three patients.Results All patients had undergone resectio n of the abnormal parathyroid.Three were parathyroid adenomas and two defined a s hyperplastic glands.All of them had pain relief after operation.The average follow-up was 15months(3-27months);the bone lesions have been repaired partially or totally s hown in X -ray examination during follow-up.Conclusion Clinically,in younger patients with multiple bone resorptions,hyperparathyroidism should be highly suspected,so that timely parathyroid surgery can be ca rried out for the treatment.[

Objective To evaluate method of CT simulation (CT sim) in the treatment planning setup of esophageal carcinoma. Methods From Dec.1999 to Jun.2002, 49 patients with pathologically proved esophageal carcinoma were analyzed. All patients underwent CT sim and treatment planning was configured with three symmetrical fields in the isocenter mode. A few comparisons have been made between CT sim and conventional simulation (Con sim) in the treatment planning setup. Results The difference in isocenter position were observed between CT sim and Con sim with 3～18 mm.When the esophageal lumen was taken as portal center, the 90% isodose curve in 42.8%(21/49) of patients were able to cover the whole lesion. When taking the tumor as the portal center, the whole lesion of all patients was totally covered by the 90% isodose curve. Conclusion The CT sim has an advantage in the delineation of lateral extension of esophageal tumor than the Con sim .Selection of irradiation portal should be done according to the size the silhouette of the tumor.

Objective To analyze the CT features of large adrenal adenoma and to compare the pathological features of large adenoma and small adenoma in order to improve the diagnosis of large adenoma.Methods The unenhanced and enhanced CT images of 14 patients with large adrenal adenoma,proven surgically and pathologically and pathological features of small adrenal adenomas in 30 cases were retrospectively reviewed.The pathological features were evaluated in the cell composition,the incidence of cystic degeneration and hemorrhage of large adenoma and small adenoma.Results 14 large adenomas were unilateral and single,3 had distinct margin and 11 had indistinct margin,14 showed regular contour of round or ellipse,the size of tumors were 5 cm?5.5 cm～18 cm?20 cm.14 large adenomas presened heterogenous density in unenhanced CT study with CT value of-36～51 HU,and 3 showed calcification and 6 showed cystic degeneration.Most of the 14 adenomas presented heterogenenous enhancement after contrast administration.Pathologic examination indicated that the clear cells were predominant in the cell composition of small adenoma,while in the case of large adenoma,the proportion of compact cell predominance was equivalent to that of the clear cell and compact cell concomitance.3 small adenomas and 6 large adenomas had cystic degeneration,5 small adenomas and 4 large adenomas had hemorrhage respectively.Statistical analysis suggested that large adenoma had higher incidence of degeneration than that in small adenoma,while no difference was found in the cell composition and incidence of hemorrhage between large adenoma and small adenoma.Conclusion The large adrenal adenomas have higher incidence of degeneration than that in small adenoma,while no difference is found in cell composition and incidence of hemorrhage between large adenoma and small adenoma.

OBJECTIVE:To detect and analyze the drug resistance of the AmpC and the extended-spectrum ?-lactamases(ESBLs)in Escherichia coli(E coli)from urinary infections.METHODS:ESBLs was detected by Phenotypic Confirmatory Test and AmpC was detected by improved three dimensional test.RESULTS:The isolating rates of ESBLs(45 strains),AmpC(8 strains)and AmpC+ESBLs in E.coli(5 strains)were 34.6%,6.2% and 3.8%,respectively.The drug resistance rates of ESBLs and AmpC-producing E.coli was higher than those of the strains without producing ESBLs or AmpC.CONCLUSION:Production of AmpC and ESBLs in ?-lactam antibiotics was the main resistance mechanism against E.coli.Carbopenems should be regarded as the first choice in clinical empirical medication for enzyme-producing strains.