Cytological characteristics of a case of adult T-cell leukemia were studied by detection of morphologic, cytochemical and immunological methods. The leukemic cells found in the peripheral blood belonged to mature T-lymphocytes and presented the changes of polymorphous nucleus which had diagnostic significance in association with clinical features and positive ATLA antibody. The mechanism of formation of cytological characteristics of adult T-cell leukemia is also discussed.

Inhibition of proliferation and induction of differentiation by 1, 25-dihydroxyvitamm D3 of human promyelocytic leukemic cell line (HL-60)are reported. At the concentration range of 109 to 106 mol/ L, 1, 25-dihydroxyvitamin D3 had effect in inhibiting cell proliferation with time-dependent and dose-dependent manner. Strong inhibition of colony cell growth (74.8?11.1%)was observed at 107 mol/L concentration. It was also found that HL-60 cells could be induced into monocytes by 1, 25-dihydroxyvitamin D3at the same concentrations,which were demonstrated by cytochemistry and specific antigen of cell surface and NBT reduction test. In addition, the relationship between proliferation and differentiation is discussed according to the result of flow cytometry.

HSP70 Heat-Shock Proteins ; analysis ; Hemorrhagic Fever with Renal Syndrome ; metabolism ; pathology ; Hepatitis Antigens ; analysis ; Hepatitis, Viral, Human ; immunology ; pathology ; Humans ; SARS Virus ; isolation & purification ; Severe Acute Respiratory Syndrome ; pathology ; virology

Antibodies ; immunology ; metabolism ; China ; Drug Industry ; history ; History, 20th Century ; History, 21st Century ; Immunohistochemistry ; history ; methods

In order to probe into the mechanism of low-dose chemotherapeutic agents in the treatment of leukemia, we studied the effects of low concentrations (l-40ng/ml) of daunorubicin (DNR) on proliferation and differentiation of human promyelocytic leukemia cell line (HL-60). 1 ng/ml DNR could inhibit the proliferation of the cells, but no induction of differentiation was found. DNR at more than 5 ng/ml had both cytotoxicity and induction of differentiation. Through the count of survival cells, [3H]TdR and [3H]UR incorporation, cloning efficiency, cell morphology and NBT reduction test, it is concluded that low-dose DNR could have inhibitory and cytotoxic effects on the cells, and its inducing differentiation was not important.

The effects of 1, 25 (OH)2D3 in combination with low-dose chemotherapeutant on the human promyelocytic leukemic cells (HL-60) were investigated. In 4-day-cultured cells, 1, 25 (OH)2D3 and low-dose harringtonine (HH) or daunorubicine (DNR) had synergic effect in inducing differentiation of HL-60 cells, and HH or DNR significantly potentiated the effect of 1, 25 (OH)2D3 in inhibiting the cell proliferation, which were demonstrated by NBT reduction test and [3H]-TdR incorporation, respectively. Cell cycle analysis by flow cytometery revealed that the cells in S phase decreased remarkably and most cells stopped at G0/G1 phase. The results provide a new way for the inducers in treatment of leukemia.

Objective To study treatment method that improve the cronic perapical periodonitis. Methods To fill the tooth channal of chronic perapical periodonitis with a paste of doxycycline and tinidazol, or gutta percha point. Results Among 206 cases of teeth those succeesful rates of two methods were 97.09 % and 98.06 % after 2 years clinical observe. Conclusion To fill the tooth channal of cronic perapical periodonitis with a paste of doxycycline and tinidazol to treat the chronic perapical periodonitis,it shortens the treatment,simplifies operation,makes application more extensive and its nearly period effect is the same as that of using gutta percha point to fill the tooth.

Insulin resistance and islet ? cell dysfunction are well-identified pathogenesis of type 2 diabetes. The generation of a great quantity of mitochondrial reactive oxygen species (ROS) impairs the function of mitochondria and induces oxidative stress during metabolic process of hyperglycaemia and hyperlipemia. Oxidative stress blocks insulin action pathway and induces insulin resistance; inhibit expression of insulin gene; decrease insulin secretion and impair ? cell function. In this paper, the mechanism of the ROS generation, insulin resistance and ? cell dysfunction induced by oxidative stress are reviewed.

Müller cells, as the special radial glial cells in the retina, span the entire retina, contact with neurons, microvessels and processes in the retina and play a significant role in protecting retinal structure and function.Diabetic retinopathy (DR) is a major ocular complication of diabetic patients.In the progression of DR, diabetic macular edema (DME) is the main cause of vision loss.During the occurrence of DME, the morphological and structural changes of Müller cells including severer swelling and vacuolation of cell bodies, increased apoptosis, and abnormal secretion of cytokines, etc.damage the blood-retinal barrier (BRB), increase the permeability of BRB and accelerate the exudation of subretinal fluid.In addition, Müller cells can disrupt the regulation of K + and water transportation, obstruct the fluid absorption in the subretinal space and promote the formation of DME.However, in the early stage of DR, neurotrophic factors secreted by Müller cells can protect the retina by alleviating retinal edema and protecting optic ganglion cells, suggesting that Müller cells can be used as targets for DME treatment.Therefore, new strategies can be provided by fully exploring the role and mechanism of Müller cells in the formation of DME.In this paper, the mechanism of Müller cells in DME and its protective role in the progression of DME were reviewed.