1.Construction of mcpr1gene vector and expression of mcpr1 in escherichia coli
Dongying XUAN ; Yan JIN ; Ming JIN ; Kun XUAN ; Rong ZHANG
Journal of Practical Stomatology 2000;0(05):-
Objective: To construct mcpr1prokaryoti c expression vector and to express MCPR1 protein.Methods:PCR was used to obtain coding region of mcpr1. Construction of a high-level fusion protein expression vector pGEX-4T-mcpr1 was conducted by inserting the fra gment of coding region of mcpr1into a fusion protein expression vector pGEX -4T-1. Then the recombinant plasmid was transferred into E. colito prepar e the MCPR1/GST fusion protein. DNA sequencing and endonucleases digesting were used to check the coding region. Results:pGEX-4T- mcpr1 wa s constructed successfully and the coding region was inserted into the vector co rrectly. A new protein band of 36 000 was observed by SDS-PAGE analysis after i nduction by IPTG. The 36 000 protein amounted to 39 percent of the total prote in and existed mostly in precipitation of broken bacteria. Conclusion: MCPR1 protein can be expressed in E. coliexpression system and purif ied initially.
2.Acute renal failure induced by primary hyperuricemia in children: a case report.
Yan LIU ; Bi-li ZHANG ; Xuan ZHANG
Chinese Journal of Pediatrics 2005;43(7):525-525
Acute Kidney Injury
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blood
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diagnosis
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etiology
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Child, Preschool
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Humans
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Hyperuricemia
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blood
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complications
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Kidney
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pathology
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Magnetic Resonance Imaging
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Male
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Uric Acid
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blood
3.A comparison between laparoscopic and open resection for large intramural hysteromyoma
Yan WANG ; Xuan WANG ; Lihua ZHANG
Chinese Journal of Minimally Invasive Surgery 2001;0(06):-
Objective To evaluate the clinical significance of laparoscopic resection for large intramural hysteromyoma. Methods A retrospective analysis was made concerning 42 cases of single intramural hysteromyoma as large as 6~10 cm in diameter: 24 cases underwent laparoscopic hysteromyomectomy and 18 cases received open resection. Intra- and post-operative parameters between the two groups were compared. Results All operations in the two groups were successfully completed without complications. The operative time was significantly longer in the laparoscopic group (89.0?26.9 min) than that in the open group (63.3?20.1 min) ( t=3.400,P =0.002). No statistical difference was observed in the intraoperative blood loss between the laparoscopic group (93.6?65.9 ml) and the open group (100.0?48.7 ml) ( t=-0.347, P=0.731) . The analgesic requirement was less in the laparoscopic group (2 out of 24 cases ) than that in the open group (9 out of 18 cases) ( ? 2 =7.208, P =0.007). The time to first flatus was shorter in the laparoscopic group (23.5?11.3 h) than that in the open group (32.0?13.6 h) ( t=-2.211, P =0.033). The postoperative pyrexia rate was significantly lower in the laparoscopic group (2/24) than that in the open group (7/18) ( ? 2=4.033, P =0.045). Conclusions Laparoscopic resection for larger intramural hysteromyoma is safe and reliable. As compared with open hysteromyomectomy, it offers more rapid recovery and lower postoperative pyrexia rate, as well as the same amount of blood loss. Its prolonged operative time may be associated with the relatively large size of the hysteromyoma.
4.Effect of inducible nitric oxide synthase on the occurrence of ischemic cerebral stroke in passive smoking female
Hongmei YUE ; Xuan ZHANG ; Zhongrui YAN
Journal of Clinical Neurology 1993;0(03):-
Objective To study the effect of inducible nitric oxide synthase(iNOS)on the occurrence of ischemic cerebral stroke in passive smoking female.Methods 100 passive smoking female patients with ischemic cerebral stroke(passive smoking group) according to the passive smoking index were divided into sub-groups index 900;25 cases in each group.The level of serum iNOS and the situation of carotid atherosclerosis were detected.The resules were compared with healthy female who without smoking and passive smoking(normal control group).Results Compared with the normal control group,the levels of serum iNOS,the intimal media thickness(IMT)s of carotid artery and the positive rates of carotid atherosclerosis plaque in each passive smoking index sub-group were significantly increased(all P
5.Intervention effect of leukotriene receptor antagonist on adriamycin-induced nephropathy rats
Yan LIU ; Bili ZHANG ; Wenhong WANG ; Xuan ZHANG ; Juan WANG
Chinese Journal of Applied Clinical Pediatrics 2014;29(17):1305-1309
Objective To explore the effect of leukotriene receptor antagonist (LTRAs,montelukast) on serum indicators,urine indicators,renal pathology,intercellular adhesion molecule-1 (ICAM-1),nephrin and podocalyxin on renal tissue in adriamycin(ADR)-induced nephropathy(ADN) rats.Methods ADN was induced through a tail intravenons injection of ADR.The 40 healthy male Wistar rats were randomly divided into 5 groups:group A (control group),group B(ADN group),group C(prednisone-treated group),group D(montelukast-treated group) and group E (prednisone and montelukast-treated group).Twenty-four-hour urinary protein (24 h-up) and serum index were measured serially in the 4th,8th week and the values of creatinine clearance rate(Ccr) were calculated.At the end of the 8th week,all rats were sacrificed to collect kidney tissues for microscopy observation of renal pathological changes.The expression of nephrin,podocalyxin and ICAM-1 in renal tissues were detected through immunohistochemistry method.Results Compared with group A,in the 4th week,24 h-up and albumin reached the level of ADR nephropathy model.Compared with group B,in the 8th week,24 h-up,cholesterol,serum creatinine and renal pathology changes in the 3 treated groups were significantly reduced (P < 0.05),but serum total protein,albumin and Ccr significantly increased (P < 0.05),while extracellular matrix/glomerulararea and renal pathology score significantly reduced (P < 0.01),and ICAM-1 significantly decreased(P <0.01).Compared with group C and group D,expressions of nephrin and podocalyxin in group E were the highest.Besides,the curative effect of integrated treatment was better than other treatments.Conclusions Maybe through inhibition of ICAM-1,LTRAs had a protective effect on renal tissue.The integrated treatment of LTRAs with prednisone has a synergistic effect and drug effect is superior to any drug alone in ADN.
6.Study of drug distribution in tissue during treatment with targeting and continuous interstitial chemotherapy in the oral squamous cell carcinoma
Yunze XUAN ; Lei ZHENG ; Jianguo ZHANG ; Jie ZHANG ; Yan GAO
Journal of Practical Stomatology 2000;0(05):-
Objective:To study the drug distribution in the local tissues and lymph nodes treated with targeting and continuous interstitial chemotherapy for the oral squamous cell carcinoma before operation. Methods:8 patients with oral squamous cell carcinoma were treated by implanting the sustain-released cisplatin around and into the primary lesion. Drug distribution in tissue were studied by the graphite furnace atomic absorption spectrometry. Results:The drug concentration in the center of the tumor,1 cm and 2 cm from the tumor fringe were (34.877 1?15.720 8),(20.690 0 ?15.688 6) and (6.635 7?3.862 3) ?g/g, respectively. The drug concentration within lymph nodes of the sub-mental and submandibular area, deep cervical lymph nodes for the upper,middle and lower were(2.991 4?3.055 7),(4.026 0 ?3.692 0), (7.192 0 ? 8.958 7) and (5.028 5?3.484 3)?g/g, respectively. Conclusion:Targeting and continuous interstitial chemotherapy for the oral squamous cell carcinoma by implanting sustain-released cisplatin can increase drug concentration in the local tissues and the lymph nodes, to attain good efficacy of targeting chemotherapy.
7.Mechanism of protective effect of sulforaphane against radiation-induced lung injury in mice
Wei XUAN ; Shuai LI ; Xiuyan WU ; Yan GENG ; Zhuo ZHANG
Chinese Journal of Radiological Medicine and Protection 2017;37(4):251-258
Objective To investigate the radioprotective function and its mechanism of Sulforaphane (SF) in mice acute radiation-induced lung injury.Methods Totally 40 female C57BL/6J mice were equally divided into 5 groups randomly.Group A,treated by SF 3 mg/kg plus radiation;group B,treated by SF 5 mg/kg plus radiation;group C,treated by SF 10 mg/kg plus radiation;radiation group with a single dose of 12 Gy in 6 MV X-ray by a linear accelerator,and control group with sham radiation.The mice in drug group were administered intraperitoneally with different concentration of SF every other day from 7 d before irradiation to 7 d after irradiation,while the same volume of DMSO plus physiological saline solvent was given in the control and radiation groups.After being sacrificed at 14 d of SF administration,the pathomorphological changes of mice were observed in trauma lung tissue,the positioning and expression of NLRP3 was observed by immunohistochemical staining,the levels of IL-6,TNF-α and TGF-β1 in bronchoalveolar lavage fluid (BALF) were measured by ELISA,the expressions of NLRP3 and IL-1β mRNA in lung tissue were assayed by qRT-PCR,the expressions of NF-κB p65,NLRP3 and IL-1β proteins in lung tissue were assayed by Western blot,the activity of NF-κB was detected by EMSA.Results In comparison with radiation group,there was an obvious amelioration in pathological injury of lung tissue in the treatment groups:the expression of NLRP3 in lung tissue decreased;the concentration of NLRP3 in the drug intervention group (SF 10 mg / kg) markedly decreased (F =42.750,P < 0.05).the IL-6,TNF-a and TGF-β1 levels in BALF decreased (tIL-6 =-62.65-21.00;tTNF-α =-32.18-16.57;tTGF-β1 =-58.22-46.11,P < 0.05);the expressions of NLRP3 and IL-1β mRNA markedly decreased (tNLRP3 =-6.56-5.68;tIL-1β =-29.75--21.20,P < 0.05),and the expressions of NF-κB p65,NLRP3 and IL-1β proteins decreased (tNF-κB p65 =-34.00--1.71,tNLRP3 =-25.01--16.91,tIL-1β =-73.70--55.14,P < 0.05);the relative expressions of NF-κB p65 and NLRP3 were reduced in a dose-dependent manner (r =0.945,0.926);and the activity of NF-κB were obviously reduced (tNF-κB =-38.68,-614.82,-2 831.40,P < 0.05).Conclusions Sulforaphane effectively alleviates the RILI in lung of mice by downregulating the expressions of inflammatory factor NLRP3.
8.The spectra and the change of diseases in AIDS patients after antiretroviral treatment in Henan province
Xuan YANG ; Xue ZHANG ; Chaofeng LI ; Yan SUN ; Qingxia ZHAO
Chinese Journal of Primary Medicine and Pharmacy 2015;(14):2118-2121
Objective To explore the change and the spectra of opportunistic infections in AIDS patients after antiretroviral treatment in He′nan province.Methods Inpatients of post treatment with disease in accordance with the criteria were selected from the Sixth People′s Hospital of Zhengzhou.A retrospective study was carried out, including the content such as gender,age,infection route,treatment time,the date of complication,and CD4+T lympho-cyte counts when the patient with complications.Disease were divided into four group:opportunistic infections,adverse drug reactions,tumor,cardio-cerebrovascular disease and diabetes mellitus.Statistic case number and incidence of every kinds of the disease in the different stages of HAART.Results (1)1 001 cases of patients were involved in the result analysis.People of 41-60 years old,the peasants and peasant workers,accounted for 57.4%and 78.9%.The infection route was mainly through blood transmission and blood collection, accounting for 83.45%.Most of the patients come from Zhumadian, Xuchang, Zhoukou, Nanyang, which were high -incidence area in He′nan.( 2 ) In 1 001 patients of post treatment, the rate of opportunistic infections was 58.4%, while adverse drug reactions was 30.5%.Bacterial pneumonia,extrapulmonary tuberculosis,oral cavity fungal infection and tuberculosis were common in opportunistic infections of patients of post treatment.The main adverse reactions were allergic dermatitis,liver injury and myelosuppression.The liver cancer case was the highest ( 8/29 ) .( 3 ) 1 001 cases were divided into 6 groups according to different count of treatment time,during 3 months of treatment,the incidence rate of opportunistic infec-tions and adverse drug reactions was the highest,accounted for 75.2% and 41.6%.After 5 years of treatment,the incidence rate of cardio-cerebrovascular disease and diabetes mellitus was 6.39%.With the prolonging of HAART time,incidence of opportunistic infections and adverse drug reactions remarkably reduced,but the occurence rate car-dio-cerebrovascular disease and diabetes mellitus increased.Conclusion In AIDS patients after HAART in He′nan at present,opportunistic infections and adverse drug reactions are main diseases and used to occur during three months after HAART.Attention will be focused on tumor, cardio -cerebrovascular disease and diabetes mellitus in AIDS patients of long-term survival.
10.Evaluation of the efficacy of immunosuppressive therapy with rabbit-antithymocyte globulin combined with Cyclosporine in children with severe aplastic anemia
Yan SU ; Runhui WU ; Jie MA ; Xuan ZHOU ; Rui ZHANG
Chinese Journal of Applied Clinical Pediatrics 2015;30(3):189-193
Objective To evaluate the efficacy and adverse reaction of the first line therapy with rabbit-antithymocyte globulin (ATG) combined with Cyclosporine (CsA) in children with acquired severe aplastic anemia.Methods A retrospective analysis was performed.The clinical data included 44 children with acquired severe aplastic anemia treated with ATG combined with CsA in Beijing Children's Hospital Affiliated to Capital Medical University between June 2006 and December 2013.The SPSS 19.0 statistical package was used for data analysis.Results Sixteen cases were severe aplastic anemia,and 28 cases were very severe aplastic anemia.The median age of treatment with ATG was 8 years old and 10 months.The median follow-up period was 24 months.The hematologic response rate was 66% (29/44 cases) in 6 months after ATG treatment,and the overall response rate was 77% (34/44 cases) at the endpoint of follow-up.The median time of partial remission (PR) was 3 months,of complete remission (CR) was 12 months.Eighty-five percent (29/34 cases)effective cases achieved PR during 6 months,while 56% (15/27 cases)cases achieved CR in 12 months.There was no significant difference between the response rate with ATG treatment and gender,severity of disease,age,interval from clinical onset to ATG treatment,and ratio of CD4 +/CD8 +.The response rate in children without serum sickness was superior to that with serum disease(P =0.006).Thirty-four cases (77%) manifested immediate side-effects with ATG infusion.The side-effects included fever,chill,rash,gastrointestinal distress and irritable cough.The overall 2-year survival rate was 87.3% (39/44 cases) and the 2-year transplantation-free survival rate was 82.5% (37/44 cases) (x2 =0.34,P =0.56).Conclusions The response rate with rabbit-ATG combined with CsA in children with acquired severe aplastic anemia was 77%.The adverse reactions were minor.The immunosuppressive therapy should begin as soon as possible in children without chance for hematopoietic stem cell transplantation.