Objective To observe the retinal toxicity of repeated intravitreal injection with bevacizumab(Avastin)in diabetic rats.Methods Forty male Sprague Dawley(SD)rats were randomly divided into normal group(Group A,10 rats)and diabetes mellitus group(30 rats).The rats in diabetes mellitus group were induced with streptozotocin injection for diabetic retinopathy model.And then randomly divided into diabetic retinopathy(DR)group(Group B,10 rats),the rats were not intervened;the left eyes of the other 20 rats were intravitreal injected with bevaeizumab 3 μ1(25 mg/m1)for 3 times as experimental group(Group C);the right eyes of the 20 rats were not intervened as experimental control group(Group D),20 days after last intravitreal injection,retinal function was measured by Flicker Electroretinogram (F-ERG);retinal vascular pattern was determined by fluorescence microscopy of ethidium bromide(EB)stained retinal flat mounts;retinal morphological changes were determined by light microscope on hematoxylin-eosin (HE) stained sections;Thy-1 and VEGF expression was measured by immunohistochemistry staining.Results F-ERG showed that-the differences of a-and b-waves-the b-wave amplitude and the Ops-wave amplitude in the implicit time between group A,B,C and D were significant (F=33.165,36.162,19.955,23.243;P=0.000);the differences of a-wave amplitude between group A,B,C and D was not significant(F=0.097,P=0v961).Retinal blood vessel pattern was normalin Group A;retinal vascular vessels were tortuous and irregularly expanded in Group B:retinal vascular vessels of Group C were regular and thinner than Group A;microaneurysm were showed in Group D.Light microscope displayed that the layers of the rat retina of Group A were regular,the retinal architectures of Group B were irregular,the retinal layers were regular in Group C,the retinal layers were irregular in Group D.Immunohistochemistry staining discovered that Thy-1 and VEGF were mainly expressed in ganglion cell layer(GCL).Conclusion Repeated intravitreal injection of bevacizumab iS toxic tO retina of diabetes mellitus rats.

[Objective]To disclose the mechanism of Leweiyin treating functional dyspepsia(FD) through experimental research. [Methods]Wistar rats are randomly divided into 7 groups: normal control, natural recovery, Madinlin, Shenling Baizhu pill, low dosage of Leweiyin, mediate dosage of Leweiyin, high dosage of Leweiyin, 6 in each group. Respectively administer to them with distilled water, Madinlin, Shenling Baizhu pill liquid, low, mediate and high dosage of Leweiyin at 2ml/100g, once every day, successively administer for 14 days one day after self model-making. Observe the gastric mucosa,measure gastro-intestinal hormones SP and VIP. [Results] Leweiyin can effectively increase SP and decrease VIP in experimental rats gastric antrum. [Conclusion] Leweiyin can increase gastro-intestinal tract excitement, regulate the contractility of gastro-intestinal smooth muscle, which may be the main mechanism for Leweiyin to treat FD.

Objective To compare the long-term prognosis of patients with stage Ⅰ endometrial cancer (EMC) after laparoscopic surgery or open laparotomy. Methods Totally 83 patients with stage Ⅰ EMC who underwent laparoscopic surgery or open laparotomy between 1993 and 2008 were enrolled in this study. The clinical data were analyzed retrospectively to compare the survival time during long-term follow-up between the two groups. Results The total survival rate was 95.2% (79/83) in the 83 cases. The median survival time was 77,51,and 31 months respectively in stages Ⅰa,Ⅰb,and Ⅰc EMC patients. No significant difference was found in the survival rate between open and laparoscopy groups [94.2% (49/52) vs 96.8% (30/31),Z=0.028,P=0.978]. For the stage Ⅰa,stages Ⅰb and Ⅰc,and G2 and G3 patients,the median survival time was similar between the open and laparoscopy groups (80 month vs 63 months,P=0.483; 49 months vs 48 months,P=0.781; and 51 months vs 49 months,P=0.635). However,in the G1 patients,significantly difference was detected in the median survival time between the two groups (77 vs 51 months,P=0.037). Conclusion Laparoscopic surgery should be used as a routine surgical approach for patients with stage Ⅰ EMC considering its minimal invasiveness and promising prognosis.

Objective To evaluate the effect of CO2 pneumoperitoneum with various pressures on the ultrastructure of the implanted lesions in rats model of endometriosis. Methods Endometriosis model was established in 30 SD rats by implanting self-endometria onto the peritoneum to form ectopic endometrial tissues. Then the animals were divided randomly into three groups (ten in each):control,10 mm Hg,and 20 mm Hg CO2 pneumoperitoneum groups (CO2 inflation lasted for 1 hour). One week after the procedure,the ectopic endometrial tissues were sampled for electron microscopy to determine the ultrastructure of cell organelle in the endometriosis glands and matrix cells. Results The electron microscopy showed that in the control group,the matrix cells lined up in order and the structure of the microvilli (MV) was normal; while in the 10 mm Hg and 20 mm Hg groups,the chromatin margination of glandular cells and mitochondria swelling were observed with reduced or vanished mitochondrial crista,the ultrastructural damages to the microvilli (reduced or vanished) were more apparent than the control,the cell junctions were widened or collapsed,and the matrix cells lined up disorderly. Compared to the 10 mm Hg group,ultrastructural damage to the glandular cells in the 20 mm Hg group was more severe. Conclusions CO2 pneumoperitoneum intervention can induce ultrastructural damage to endometriosis lesions. 20 mm Hg pressure of CO2 pneumoperitoneum can induce severer damage to the ectopic endometriosis tissues than 10 mm Hg.

Aged ; Female ; Foreign Bodies ; pathology ; Humans ; Laryngeal Diseases ; Larynx ; pathology ; Vestibule, Labyrinth

Objective To know the feasibiliy and the effects of shorten preoperative fasting time for patients with digital cerebral angiography. Methods Divided 110 patients with digital cerebral angiogra-phy into the observation group and the control group according to their sequence enter the hospital. Routine fasting method was used in the control group, shorten fasting time was used in the observation group, com-pared the incidence rate of fasting-related complications between the two groups. Results The incidence rote of belly discomfort, thirstily, strvation and hypoglyeemic during the digital cerebral angiogrsphy in the con-trol group was significant higher than those of in the observation group, while there were no significant difference about the incidence rate of postoperative complication between the two groups. Conclusions For patients with digital cerebral angiography can shorten the preoperative fasting time properly, which can avoid certain complieaitons.

Objective　To assay serum cortisol concentration in type 2 diabetic patients and evaluate the relationship between serum cortisol concentration and insulin resistance. Methods　Serum cortisol concentrations were measured at 8 am, 4 pm and during oral glucose tolerance test(OGTT) in 60 newly-onset type 2 diabetic patients and 40 healthy subjects using RIA. Results　① Sernm cortisol concentrations were elevated in obese type 2 diabetic patients at 8 am and 4 pm as compared with non-obese patients and normal controls. ② Changes of serum cortisol concentration during OGTT were different between obese type 2 diabetic and non-obese patients: serum cortisol concentrations were decreased after glucose administration in non-obese patients but not in obese patients. ③ In type 2 diabetic patients, serum cortisol concentrations were negatively correlated with insulin sensitivity index (r=-0.34, P<0.01). Conclusion　There is some degree of abnormal in cortisol secretion in obese type 2 diabetic patients.

The molecular targeted therapy method using microRNAs(miRNAs)is gradually stepping into people′s vision. miRNAs affect colorectal cancer progress via abnormal expression in tumor cells or micro-environment. The high or low expressions of miRNAs in specific tissues probably have an impact on the expressions of oncogenes,tumor suppressor genes or other aspects including the surrounding environments,the metastases and the drug tolerance of tumors,thus contributing to curing the colorectal cancer. Nowadays, miRNA has entered the stage of animal experiments.