Objective: To investigate the characteristics of lower respiratory tract infection caused by Chryseobacterium and its antimicrobial susceptibility in vitro . Methods: The clinical data of 52 cases of lower respiratory tract infection caused by Chryseobacterium were analyzed, and the antimicrobial susceptibility of commonly used agents was determined with the method of agar dilution. Also the 50% lethal dosage (LD 50 ) (as the marker of virulence) of 20 randomly selected Chryseobacterium strains for mice were determined. Results: (1) Thirty six was over 60 years old;all of 52 cases had underlying diseases, mainly were chronic obstruction pulmonary disease and malignant tumors. Seventeen cases had the history of incubation or tracheotomy for mechanical ventilation, and 35 had history of broad antibiotics treatment. The mean hospitalization time before infection were 35.6 d, and 38.5% of the cases had mixed infection with other bacteria. No specific clinical manifestations and chest X ray appearance revealed. (2) The in vitro activity of 25 agents showed that these strains were highly resistant. (3) The range of the LD 50 of tested strains was 4.11?10 6 5.68?10 8/mouse, suggesting low virulence of this kind of bacteria. Conclusion: The lower respiratory tract infection caused by Chryseobacterium has no unique features; the incidence of the infection increases in immunosuppressed old patients with various underlying diseases, although the virulence is relatively low. Because the clinical isolates are highly resistant, the antibiotics should be selected according to the results of bacterial sensitivity test.

Objective:To investigate the preventive and therapeutic effects of intraperitoneal injectoin of IFN? on bronchial asthma in mice and the relevant mechanism. Methods: Thirty-six BALB/c mice were randomly equalized into 3 groups:group A (normal control group),group B (asthmatic model group) and group C (IFN? treated group). The asthmatic model was established in group B and C by immunization with ovalbumin (OVA) absorbed to aluminum hydroxide. Mice of group B and C received 0.25 ml PBS and 5 ?g IFN? intraperitoneally on days 23 to 30 once daily prior to ovalbumin challenge,respectively. Bronchoalveolar lavage fluid (BALF) was collected on day 31 for determining the cellular composition and the concentrations of IL-4 and IL-5. Meanwhile,IgE in serum was determined. The pathological changes and the expression of GATA-3 were investigated in the lungs of mice. Results: (1) BALF eosinophils was significantly decreased in group C compared with those in group B ( vs ,P

Objective To investigate the role of IFN-? in prevention and treatment of bronchial asthma and the mechanism of its effect. Methods BALB/C mice were randomly divided into three groups: group A (control group, n=12); group B (asthma model group, n=12); group C (IFN-? intraperitoneal treatment group, n=12). The asthma model was reproduced in group B and C with ovalbumin (OVA) adsorbed to aluminum hydroxide. PBS (0.25ml) and IFN-? 5?g was respectively injected intraperitoneally in group B and C on days 23 to 30 once daily prior to ovalbumin challenge. Bronchoalveolar lavage fluid (BALF) was collected on day 31 and its cellular composition was analyzed. The levels of IL-4, IL-5 and the IgE in serum were determined. The pathological changes in the lung and the expression of GATA-3 were observed. Results A notable decrease of eosinophils (0.3?0.2) in BALF was found in group C comparing with the group B (21.1?6.7) (P

To study the treatment of metastasis of experimental lung cancer with a recombinant adenovirus carrying IL 2 gene, concentrations of IL 2 and associated cytokines in murine lung lavage and blood were determined by ELISA at different times after intratracheal injection of the recombinant adenovirus. The lung metastasis nodes, survival time, survival rate were investigated in a C57BL/6 mouse model by intratracheal administration, and the NK activity and CTL activity were also determined by 51 Cr 4 hour release method. The results showed that IL 2 could be detected in lung tissue as early as 6 hours after administration of IL 2 gene, its concentration was higher in lung lavage than in peripheral blood, and was higher in experimental group than that in the control group; intratracheal use of the IL 2 carring recombinant adenovirus had significant therapeutic effect on metastasis of experimental lung cancer. Increased CTL and NK activities, longer survival time, and higher survival rate were observed in the experimental group compared with the control group. It is suggested that intratracheal usage of adenovirus vector carrying IL 2 gene might play therapeutic role on the lung cancer metastasis, indicating that gene therapy of lung tumors could be done through airway directly with recombinant adenovirus.

Objective To analyze the etiology of diabetes ketoacidosis(DKA) in children with type 1 diabetes.Methods Totally 850 person-time of type 1 diabetes children in recent 20 years in our hospital were selected as studied subjects. Two hundred and twenty-five person-time of them were hospitalized because of DKA.Fifty-six cases (131 person-time) were due to recurrent DKA.These patients were classified into 2 groups according to onset time: group 1(diagnosed from 1982 to 1991) and group 2(diagnosed from 1992 to 2001).Results The analysis of recurrent DKA suggested that 71.8 % of them was due to infection, 20.4 % of them did not obey diabetic diet and 9.2 % of them discontinued insulin injection. The etiology of DKA showed no difference in two groups. The number of recurrent DKA in two groups was significantly different (P

Equipment Failure ; Female ; Heart Ventricles ; pathology ; surgery ; Humans ; Middle Aged ; Pacemaker, Artificial

Objective 　To investigate the effect of risperidone on regional cerebral blood flow (rCBF) and the relationship between efficacy and rCBF ratio. Methods　Twenty four naive schizophrenic patients (diagnosed according to the ICD 10) completed 8 weeks treatment with risperidone. Ten patients were male and 14 were female. Twenty six healthy controls were enrolled as control group. The treatment dose of risperidone was 3～6 mg/d. After 8 weeks treatment, brain imaging was conducted again. Results　Before treatment with risperidone, compared to the control group, the baseline rCBF ratios of left and right inferior posterior temporal of patients were higher and the cognitive activated rCBF ratios of left mid-lateral frontal was lower. After treatment, the baseline state rCBF ratios of right lateral temporal, left and right superior posterior temporal were significantly decreased. The cognitive activated rCBF ratios of left and right inferior medial frontal, left inferior lateral frontal, left superior fronto temporal and left superior lateral fronal significantly increased. The efficacy was correlated with changes of the baseline rCBF ratio in some RIOs. Conclusions　Risperidone could change the blood perfusion in some ROIs. It suggested that the perfusion in these ROIs could be useful for predicting treatment efficacy.

Objective: To investigate the effect of CpG motif oligodeoxynucleotides (CpG ODN) on the antigen induced allergic airway reaction in mice. Methods: The asthma model was set up in the C57BL/6 mice with OVA, the CpG ODN in the dose of 30 ?g was co administered intraperitoneally with the antigen in sensitization stage to study its effect on the airway allergenic reactions. Results: (1)Compared with the control, coadministration of CpG ODN in sensitization phase significantly inhibited airway eosinophilia after antigen challenge( P

Objective To explore the nursing intervention on the pregnant women with syphilis during pregnancy so as to reduce the harm to maternal and baby.Methods Many information such as age,occupation,dwelling environment,marriage and sexual life,curing during pregnancy,pregnant and perinatal infant outcome from medical records were reviewed. Gestational syphilis women of 847 cases were randomly divided into nursing intervention group with 427 cases and control group with 420 cases.Nursing intervention group received one-by-one psychological care and health education,got cooperation from families,and received routine blood test and cure cooperation,while control group only received routine blood test and curing cooperation.Patients were followed up for seven days.Results The rate of adherence to treatment in nursing intervention group was 98.1％ which was significantly higher than 61.7％ in control group ( x2 =176.2,P ＜ 0.01 ),and the rate of accepting standard treatment between early and late pregnancy,middle and late pregnancy were significantly higher than that in control group ( x2 =17.8,P ＜ 0.01 ).The week was shorter for receiving nursing intervention and the patients' compliance was better ( P ＜ 0.01 ). Only 23 syphilis infection babies in the nursing intervention group were born,but 149 babies in control group were infected by syphilis,and the difference was significantly different( x2 =123.2,P ＜ 0.01 ).Conclusions Nursing intervention can effectively increase the compliance of pregnant women with syphilis and improve the outcome of pregnancy and perinatal infant.

Objective To study the effect of fluorine on the bone histomorphometry of humbar in rats.Methods Ninety 2-month-old SPF Sparague-Dawley rats,half male and female,were randomly divided into 9 groups:control[(childhood(CS),adult(AS),long-time(NS)]group and drug group[childhood high-fluoride and low-fluoride group(CHS,CLS),adult high-fluoride and low-fluoride(AHS,ALS),long-term high-fluoride and low-fluoride(CLHS,CLLS)].The control group was administered orally with solution of 0.9%NaCl,while the drug group was given orally with different dose of NaF at the same time. Sections of the fifth lumbar were made which was undecalicified for bone histomorphometric analysis, including the percentage of trabecular bone area (% Tb.Ar),trabecular thickness(Tb.Th), trabecular number(Tb.N), trabecular separation(Th.Sp) ; broken trabecular bone area cells (Oc.N), osteoclast perimeter percentage (% Oc.Pm), the percentage of labeled perimeter (% L.Pm), bone mineral apposition rate(MAR), osteoblast perimeter(Ob.PM), trabecular bone perimeter formation rate (BFR/BS),trabecular bone area formation rate (BFR/BV), the total area of bone formation rate (BFR/TV). Results [1]The percentage of Tb.Ar, Tb.Th, Tb.N,%L.Pm, MAR, BFR/BS, BFR/BV and BFR/TV of CHS group [(50.63 ±7.44)%, (150.26 ± 27.51 )μm, (3.44 ± 0.47)N/mm, (50.63 ± 7.44)%, (0.85 ± 0.03)μm/d, (8.45 ± 2.36)μm/d ×100, (381.16 ± 41.62)%/year, (75.07 ± 4.81)%/year] was higher than that of CS group [(29.71 + 9.32)%,(110.93 ± 28.19)μm, (2.68 ± 0.34)N/mm, (24.00 ± 1.22)%, (0.65 ± 0.03)μm/d, (5.43 ± 0.18)μm/d × 100,(141.32 ± 9.29)%/year, (58.14 ± 2.3)%/year, all P < 0.05)]. The %Tb.Ar, Tb.Th, %L.Pm, MAR, BFR/BS,BFR/BV, BFR/TV and Ob.PM of CLS group [(40.76 ± 6.43)%, (164.25 ± 45.65)μm, (42.02 ± 6.12)%, (0.85 ±0.04)μm/d, (8.95 ± 3.73)μm/d × 100, (378.73 ± 35.39)%/year, (73.52 ± 8.71)%/year, (1.41 ± 0.05)μm] were increased (all P < 0.05). [2]Compared with AS group, the %Tb.Ar,Oc.N, %Oc.Pm, %L.Pm, MAR, BFR/BS,BFR/BV and BFR/TV of AHS group[ (50.62 ± 5.76)%, (0.51 ± 0.05)N/mm, (1.13 ± 0.05)%, (42.3 ± 7.02)%,(1.28 ± 0.09)μm/d, (12.91 ± 1.52)μm/d × 100, (390.12 ± 43.56)%/year, (65.21 ± 22.13)%/year] was higher than that of AS group[ (42.73 ± 5.22)%, (0.41 ± 0.17)N/ram, (0.77 ± 0.52)%, (28.43 ± 6.93)%, (0.80 ± 0.03)μm/d, (9.83 ± 1.44)μm/d × 100, (324.43±53.44)%/year and(48.35 ± 9.36)%/year, all P < 0.05)] . The %Tb.At, Oc.N, %Oc.Pm, %L.Pm, MAR, BFR/BS, BFR/BV and BFR/TV of ALS group [(51.14 ± 6.22)%, (0.49 ±0.61)N/mm, (1.17 ± 0.11)%, (45.06 ± 6.92)%, (1.39 ± 0.08)μm/d, (12.87 ± 1.35)μm/d × 100, (394.6 ±50.23)%/year and(66.31 ± 18.93)%/year] were higher than that of AS group(P < 0.05) .[3] The Ob.PM ,Oc.N and %Oc.Pm of CLHS group[ (1.47 ± 0.27)μm, (0.58 ± 0.13)N/mm, (1.14 ± 0.07)%] were obviously increased(P <0.05), as compared with NS group [ (0.82 ± 1.20)μm, (0.42 ± 0.25)N/mm and (0.75 ± 0.64)%, all P < 0.05].Conclusions The short-term administration of NaF on rats in the growing period increases the bone formation and osteoblast activities of young rats and adult rats. The long-term administration of NaF on rats does not increase the bone formation of rats in growth period. The osteoblast activities as well as the bone absorption of lumbar vertebra were strengthened. The likelihood of bone fracture became larger. The negative effects on bone metabolism and bone quality of rats were gradually displayed along with the prolongation of sodium fluoride usage.