1.Evaluation on white cell count in peripheral blood and plasma C-reactive protein for risk of coronary heart disease
Caixia TIAN ; Yan LI ; Zun'En XIA ;
Chinese Journal of Clinical Laboratory Science 2006;0(06):-
Objective To evaluate CRP,peripheral WBC and neutrophil for the risk of coronary heart disease.Methods Plasma CRP levels,peripheral WBC and neutrophil count of 68 acute coronary syndrome(ACS)patients,28 stable angina(SA)patients and 33 controls were measured.Results There were significant differences of CRP levels,peripheral WBC and neutrophil count between the ACS group and the SA group or control group;CRP levels,peripheral WBC and neutrophil count in acute myocardial infarction(AMI)patients were much higher than those in unstable angina(UA)patients,SA patients and controls(P
2.Changes of TNF-alpha and C(3) complements in patients with silicosis.
Li-yan TIAN ; Jun JI ; Chun-xia YANG
Chinese Journal of Industrial Hygiene and Occupational Diseases 2007;25(9):572-572
Aged
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Aged, 80 and over
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Complement C3
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metabolism
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Humans
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Immunoglobulin A
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blood
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Male
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Silicosis
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blood
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Tumor Necrosis Factor-alpha
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blood
3.Impact of community healthcare service center in emergency response to natural disaster
Tian XIA ; Hongjun CAO ; Zhaoxi ZHOU ; Yan DAI ; Quan GONG
Chinese Journal of General Practitioners 2008;7(12):839-840
The Medical Healthcare Service Center,located at the Youxian District,Mianyang City of Sichuan Province,successfully rescued the people affected by the Wenchuan earthquake and the Tangjiashan barrier lake with great help from the senior hospitals.We suggest that the community healthcare service center could play an important role in emergency response system.
4.Research advances in neurobiological mechanism of acupuncture for amblyopia
Chong-Bing MA ; Tian-Tian ZHU ; An-Guo LIU ; Zhao-Xia CAO ; Xing-Ke YAN
Journal of Acupuncture and Tuina Science 2019;17(4):278-283
Amblyopia greatly affects the physical and mental development of children. Acupuncture is effective for amblyopia, though its mechanism remains unclear. This article summarized the mechanism of acupuncture treatment of amblyopia from the perspectives of morphology of neurons in visual cortex, visual electrophysiology, and molecular biology, etc. It was found that acupuncture may treat amblyopia through repairing the morphological and ultrastructural damages of neurons in visual cortex, promoting the electrical activities in visual pathway and visual cortical neurons, and modulating the synthesis and expression levels of factors involved in visual system. Nevertheless, further studies are required to unveil the mechanism of acupuncture treatment of amblyopia.
5.Construction and identification of adenovirus vector containing mouse CD40Ig gene
Tian XIA ; Xinghua GOU ; Lnan YAN ; Dehua LI ; Haiyang HU ; Juan YAN ; Bo LI ; Zhongrong LIU
Chinese Journal of General Surgery 1994;0(05):-
Objective To construct recombinant adenovirus vector containing mouse CD40Ig fusion gene for the study of induction of donor-specific tolerance. Methods CD40Ig fusion gene was constructed by PCR overlapping technique, and was cloned into the shuttle plasmid pAdTtrack-CMV. The linearized shuttle plasmid was co-transfected into the E.coli strain BJ5183 with bone plasmid pAdeasy1, then the recombinant adenovirus plasmid was generated. The adenovirus was packaged and amplified in Cells 293. Results The recombinant virus AdCD40Ig was successfully constructed and its titer was 5?109 efu/ml. Conclusion AdCD40Ig can be used as an agent in experiment to induce donor-specific tolerance.
6.Analysis of microvessel density in pterygium tissue with corneal laser confocal microscopyc in vivo and immunohistochemistry in vitro
Chun-yan, XUE ; Ting, ZHU ; Yuan, XIA ; Yan, WU ; Zhen-ping, HUANG ; Nong, TIAN
Chinese Journal of Experimental Ophthalmology 2012;30(1):46-49
BackgroundPterygium is an ocular surface disease of abnormal cell proliferative kind and angiogenesis plays an important role in its development and recurrence.Several anti-angiogenic therapies have been used to treat pterygium,but there very few studtes for the in vivo observation of the microvessles in pterygium.ObjectiveThis study was to observe angiogenesis in pterygium with a high-resolution confocal microscope in vivo and to perform immunohistochemical study in vitro.MethodsA prospective case-controlled study was designed.Twenty eyes of 20 consecutive patients with primary pterygia and 20 age- and sex-matched patients with inner eye diseases and strabismus with normal conjunctiva were enrolled in this study.An in vivo confocal microscopy imaging system (Heidelberg Retina Tomograph Ⅱ Rostock Cornea Module) was used to collect microvascular pictures from the anterior part of pterygia and normal nasal conjunctiva of controls,and then immunochemistry was performed to examine the expression of CD31 in microvessel in vitro.The vascular density values were compared between these two groups.The correlation of vascular density values between in vivo Heidelberg Retina Tomograph and in vitro immunohistochemistry was calculated.Written informed consent was obtained from pationts before any examination and surgery.ResultsUnder the in vivo confocal microscope,the microvessel density was (8929±2993) μm/mm2 and (4202 ±692)μm/mm2,respectively,in pterygium and the normal conjunctiva group with a statistically significant difference between them (t =6.881,P<0.01 ).Immunochemistry revealed that the expression of CD31 to measure vascular density was ( 21.00 ± 4.06/400 × field ) and ( 6.07 ± 1.75/400 × field ) in pterygium and the normal conjunctiva group,showing significant difference (t =12.312,P<0.01 ).Positive correlations were found in the vascular density values between in vivo corneal laser confocal microscopy examination and in vitro immunochemistry examination in both the pterygium group and normal conjunctiva group (pterygium group:r=0.649,P<0.01 ;normal conjunctiva group: r=0.572,P<0.01 ) ConclusionsIn vivo confocal microscopy imaging is superior to in vitro immunochemistry in evaluating the microvessel of pterygium.The results of this study offer a new way index for further investigation of the biological behavior of pterygium and its mechanism.
7.Effect of thymosin β4 on transforming growth factor-β/connective tissue growth factor of renal tubular interstitial fibrosis rats
Jing YUAN ; Yan SHEN ; Ying XIE ; Xia YANG ; Xin LIN ; Maolu TIAN ; Yan ZHA
Chinese Journal of Nephrology 2016;32(3):206-211
Objective To investigate the influence of thymosin beta 4 (Tβ4) with two different dosages on the expression of transforming growth factor beta (TGF-β) and connective tissue growth factor (CTGF) in rats with renal tubular interstitial fibrosis,and to further estimate the changes of renal tubular interstitial lesions.Methods Rat models of renal tubular interstitial fibrosis were established by unilateral ureteral occlusion (UUO).The male SD rats were randomly divided into 4 groups and 15 rats in each group:sham group,model group,treatment group with 1 mg/L Tβ4 and treatment group with 5 mg/L T34.Rats in sham group and model group were poured into the same amount of saline.The renal function and renal pathological changes were observed after the second week.The mRNA and protein expression of TGF-β and CTGF in renal tissues was tested by in-situ hybridization and Western blotting.Results Compared with that in sham group,the expression of TGF-β mRNA and its protein,CTGF mRNA and its protein was significantly higher in model group (all P < 0.01).Compared with rats of model group,Tβ4 treatment rats had lower mRNA and protein expression of TGF-β and CTGF (all P < 0.01),and the expression in treatment group with 5 mg/L Tβ4 was lower than that in treatment group with 1 mg/L Tβ4 (P < 0.05).And the expression of TGF-β mRNA was positively correlated with CTGF mRNA expression (r=0.697,P < 0.01).The 24 h total urinary protein and the area of renal tubular interstitial lesion in model group were significantly more than those in sham group,and also more than those in Tβ4 treatment group (all P < 0.05).Tβ4 treatment attenuated kidney damage,and the effects in treatment group with 5 mg/L Tβ4 were better than those in treatment group with 1 mg/L Tβ4.No difference in serum creatinine and blood urea nitrogen was observed among 4 groups (all P > 0.05).Conclusions Tβ4 treatment can inhibit the renal TGF-β and CTGF expression of rats with tubular interstitial fibrosis in a dose-dependent manner,and play a protective role in kidney.
8.Rosai-Dorfman disease of lung: report of a case.
Dong TIAN ; Da-Hua ZHAO ; Shu-Hua WU ; Xia WANG ; Yan ZHANG
Chinese Journal of Pathology 2009;38(10):705-706
Adult
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Antigens, CD
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metabolism
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Antigens, Differentiation, Myelomonocytic
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metabolism
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Carcinoma, Giant Cell
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metabolism
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pathology
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Diagnosis, Differential
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Granuloma, Plasma Cell
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metabolism
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pathology
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Histiocytosis, Sinus
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metabolism
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pathology
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surgery
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Humans
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Immunohistochemistry
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Lung Diseases
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metabolism
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pathology
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surgery
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Lung Neoplasms
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metabolism
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pathology
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Male
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Pneumonectomy
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methods
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S100 Proteins
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metabolism
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Vimentin
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metabolism
9.Differential analysis of two-dimensional gel electrophoresis profile of pancreatic ductal adenocarcinoma and adjacent tissue
Rui TIAN ; Liming WEI ; Yan LI ; Renyi QIN ; Zhiyong DU ; Wei XIA ; Hong JIN
Chinese Journal of General Surgery 1994;0(05):-
Objective To study the difference of expression of proteins in pancreatic cancer and adjacent fissue.Methods Proteomes of eight pairs of pancreatic ductal adenocarcinoma tissue samples and adjacent tissue samples were obtained by high resolution two-dimensional gel electrophoresis(2-DE).Comprehensive analyses of proteins were focused on total protein spots exhibiting statistical alternations between the two groups.Protein identification was done by peptide mass fingerprinting with tandem mass spectrometry(MS/MS).In addition,Western blotting and immunohistochemistry were performed to verify the expression of certain candidate protein.Results A total of 28 protein spot-features were found to be significantly increased and 17 significantly decreased in tumor tissues.Thirty of these protein spots were identified,which included enzymes,antioxidant proteins,signal transduction proteins,calcium-binding protein,structural proteins,chaperones and others.Western blotting and IHC further validated up-regulated expressions of one candidate protein(annexin II)in tumor tissues.Conclusions The analysis of proteomics with 2-DE on human tissue is a useful method for discovering valuable cancer marker candidates.These differential expressed proteins may serve as biomarkers for early detection and therapeutic targets to pancreatic cancer.
10.Study on Measuring Cord Blood Leptin Level and the Ralatioship between Leptin and Insulin in Premature Infants
xue-ai, FAN ; yan-min, LI ; xiao-mei, HAN ; zhao-xia, TIAN
Journal of Applied Clinical Pediatrics 1986;0(02):-
0.05).2.Neonates umbilical serum leptin concentration was positively correlated with body mass index(r=0.520 P