Unmethylated CpG motif containing oligodeoxynucleotide(CpG ODN) can induce secretion of dendritic cells,and thus presents antigens efficiently,increases expression of costimulatory molecules,causes innate immunity response and adaptive immunity response,then plays anti-tumor roles.

Objective To establish the reference intervals of thyroid hormone levels fro the women in early ,middle and late preg‐nancy in the local area .Methods Through the strict screening for routine diagnosis of pregnant women who went to the hospital , we choose 420 women with 6-37 weeks of pregnancy and 120 non pregnancy women(control group) .Chemiluminescence was used to detect thyroid stimulating hormone(TSH) ,free thyroxine(FT4 ) ,total thyroxine(TT4 ) ,free triiodothyronine(FT3) ,total triiodo‐thyronine(TT3 ) for the pregnant women in early pregnancy(0-12 weeks) ,mid‐pregnancy(13-27 weeks) ,and late pregnancy(28-40 weeks) and women in control group .Using those data ,we established reference intervals of thyroid hormone and use bilateral limits P2 .5 - P97 .5 to represent .Results The differences of serum TSH ,FT4 ,TT4 ,TT3 concentration between pregnant group and control group were statistically significant(P<0 .05);while the difference of serum FT3 concentration between pregnant group and control group was not statistically significant(P=0 .79) .The concentration of TSH was not statistically significantly different in the mid‐pregnancy compared with late pregnancy(P=0 .933) ,the reference interval can be merged as 0 .56-3 .94μIU/mL .Conclu‐sion The establishment of normal reference interval of thyroid hormone concentration in different period of pregnancy respectively is conducive to the diagnosis and treatment of thyroid disease in pregnancy .

Oligodeoxynucleotides containing CpG motifs have shown powerful immunoregulation effects, which are able to activate a variety of immune cells such as natural killer (NK) cells, monocytes,macrophages, dendritic cells(DC), B and T lymphocytes. CpG oligodeoxynucleotides (CpG ODN) can not only induce and enhance nonspecific and specific immune responses, but also regulate the type of immune responses through inducing Th1-type immune response and suppressing Th2-type immune response. DC are known to be the most powerful special antigen-presenting cells (APC) at present. It plays an important role in antigen recognition,antigen processing presenting, and T cell activation, and can kill various kinds of tumor cells. Recently, the immunotherapy based on tumor vaccines of dendritic cell has become more and more important. The research progress on dendritic cells for cancer treatment is reviewed in this article.

BACKGROUND: The application of biological response modifiers (BRMs) has become the fourth cancer treatment following surgery, chemotherapy and radiotherapy. Polysaccharide-peptide (PSP) is a biological response modifier substance playing a special role in cancer treatment, which has been used in the treatment of gastric cancer, lung cancer, esophagual can cer, etc. With good results. OBJECTIVE: To observe the effect of PSP on the immune function and quality of life (QOL) in patients receiving chemotherapy for gynecological malignancies. DESIGN: A comparative observation. SETTING: Department of Obstetrics and Gynecology, Shanghai Jiaotong University Affiliated First People's Hospital. PARTICIPANTS: Forty patients who had been operated on for gyneco logical malignancies were selected from the Department of Obstetrics and Gynecology, Shanghai Jiaotong University Affiliated First People's Hospital from June 1999 to December 2002, including 36 cases of ovarian cancer with an average age of 52 years and 4 cases of endometrial cancer with an average age of 54 years. Patients with ovarian or endometrial cancer suffered from no contraindication in chemotherapy postoperatively were enrolled, otherwise were excluded.METHODS: The 40 patients were divided into study group (n=20) and control group (n=20) by matching according to stage and age. In the study group, the patients were treated with PSP capsules in addition to chemotherapy, whereas those in the control group were treated with chemotherapy only. PAC (phenacetin, spirin, caffeine) and MFP (melphalan, 5-fluorouracil, medroxyprogesterone acetate) regimens were adopted for the ovarian cancers and endometrial cancer respectively. The experiment lasted for two months from the beginning of the second course of chemotherapy to the beginning of the fourth one.MAIN OUTCOME MEASURES: The indexes of blood immune function and QOL were observed before and after treatment.RESULTS: All the 40 patients were involved in the analysis of results. ①The average Karnofkky's scores before and after treatment were (84±9.6)and (90±8.6) in the study group (P ＜ 0.05), and (83±9.2), (80±12.8) in the control group (P ＞ 0.05). The average Karnofkky's score was 10 credits higher in the study group than in the control group (P ＜ 0.05). ② The CD4+,CD4+/CD8+ and natural killer cells after treatment in the study group were all obviously higher than those in the control group (28.14±4.10, 0.96±0.17,23.80±4.90; 23.12±3.97, 0.75±0.21, 5.32±3.90) (P ＜ 0.05, 0.01).CONCLUSION: PSP can improve the cellular immunity and QOL of patients with ovarian or endometrial cancers.

0.05).FVC increased significantly in week 6 and 12(t=2.762,P =0.008;t=2.255,P =0.029,respectively).There was no statistical significance in FEV_1/FVC between two groups.IC increased significantly in week 6 and 12(t =3.204,P =0.002;t =3.109,P=0.003,respectively).Conclusion Inhaling tiotropium increases IC in patients with stable COPD.As lung function targets judging limit extent of airflow in patients with COPD,FEV_1/FVC and FEV_1(%)are not sensitive in evaluating therapeutic effect of bronchodilators.IC is more reliable than other targets in evaluating therapeutic effect of bronchodilators.

Objective: To study the antioxidant activity of Clematis chinensis Osbeck polysaccharide(CCP).Methods:① To assay the scavenging activity of CCP on hydroxyl radical and superoxide anion in vitro.② To analyze the effect of CCP on red blood cell autoxidation hemolysis induced by H2O2 by colorimetry.③ To study the effect of CCP on acute hepatic injury of the mice induced by carbon tetrachloride.Results: CCP could eliminate the hydroxyl free radical and the superoxide anion free radical,reduce red blood cell autoxidation hemolysis induced by H2O2,raise the activities of SOD and GSH-Px in hepatic injury mice's serum and liver apparently,reduce the content of MDA and liver index notably.Conclusion: CCP has the significantly action of anti oxidation activity in vitro and in vivo which relates to removing oxygen free radical.

Adult ; Aged ; Female ; Fistula ; surgery ; Humans ; Male ; Middle Aged ; Pectoralis Muscles ; transplantation ; Pharyngeal Diseases ; surgery ; Reconstructive Surgical Procedures ; methods ; Surgical Flaps

Lots of studies implicate that pathological high-frequency oscillation (HFO) was associated with epilepsy. In recent years, new recording technologies have advanced such that, at high temporal and spatial resolutions, high-frequency oscillation can be recorded in partial epilepsy by intracranial electrodes. More and more researchers focus on the study of high frequency oscillation. The techniques, which were used to record, automatic mine, analysis and quantify high-frequency oscillation, are develop quickly. As a new biomarker of epilepsy, high-frequency oscillation can reflect the epileptic form activity. The HFO has a great value in localization of human epileptic brain and epilepsy surgery.

OBJECTIVE: To investigate the relationship between syndrome differentiation of traditional Chinese medicine (TCM) and characteristic changes of vascular endothelial function in patients with diabetic arterial occlusion (DAO) of lower extremities. METHODS: Forty patients with DAO were selected as trial group. Twenty patients among them were attributed to blood stasis syndrome (group A1), and the others were attributed to syndrome of pathogenic dampness-heat attacking the lower limb (group A2) according to syndrome differentiation type of TCM. Patients with diabetes (group B), arteriosclerosis obliterans (group C) and healthy people (group D) were observed as the control groups, respectively. There were 20 cases in each group. Endothelium-dependent dilation (EDD) and endothelium-independent dilation (EID) were measured by high resolution ultrasound in the 100 subjects and the changes of vascular tension factors were also studied. RESULTS: The results showed that EDD in group A was reduced significantly as compared with that in the groups B, C and D. The levels of vascular contractile factors such as endothelin-1 (ET-1) and thromboxane B2 (TXB2) in group A were higher than those in the groups B, C and D, while the levels of vascular dilatory factors such as nitric oxide (NO) and 6-keto-prostaglandin F1alpha(6-Keto-PGF1alpha) were declined significantly as compared with those in the groups B and D. Linear correlation analysis showed that EDD was correlated positively with the levels of NO and 6-Keto-PGF1alpha, while the levels of ET-1 and TXB2 had negative correlation with EDD. EDD and EID in group A2 were declined significantly as compared with those in group A1. CONCLUSION: Our findings indicate that endothelial dysfunction may play an important role in the pathogenesis of DAO and may be associated with syndrome differentiation of TCM.

ObjectiveTo compare the health-related quality of life (HRQOL) of patients with or without coronary artery disease (CAD) and also to determine the factors affecting their quality of life.MethodsOne hundred patients with CAD and 100 patients without CAD were included in this cross-sectional study. The patients with CAD and the patients without CAD were matched by age, sex, marital status, and income. HRQOL was measured with the generic HRQOL instrument.ResultsHRQOL scores of the patients with CAD differed significantly from the patients without CAD. Compared to the patients without CAD, the HRQOL of the patients with CAD was lower in the dimensions of mobility, hearing, breathing, elimination, usual activities, mental function, discomfort and symptoms, vitality, and sexual activity. The patients with CAD had lower HRQOLscores than the patients without CAD. In the patients with CAD, age and sex independently affected the HRQOL. Older patients and women had worse HRQOL.ConclusionCoronary artery disease negatively affects HRQOL. It is thought that interventions to increase quality of life the patients with CAD may improve their HRQOL.