1.A study on synthesis and oxidation mechanism of mono-alkyl phosphate
Sheng WANG ; Shuyun YAN ; Qingguo YE
Journal of Pharmaceutical Analysis 2009;21(3):172-175,188
Oxidation of white phosphor, us by peroxides to produce mono-alkyl phosphate in the alcoholic solution has been studied under non-and catalytic conditions. In this paper, the mechanism of the oxidation process was analyzed. The content of mono-alkyl phosphalte in the product is affected by different types of alcoholic solution and peroxide solvent. The result shows the availabulity of the following order for the activity of the peroxide solvent and alcoholic solutions-tert-butyl hydroperoxide>(di) benzoyl peroxide>hydroperoxide; methanol> n-butyl alcohol> phenol. Under optimized reaction conditions: n (a white phosphorus) : n( tert-butyl hydroperoxide) : n (methanol)= 1:10:12, reaction temperature 80 ℃, reaction time 2.5 h, products of 80.0% mono-alkyl phosphonates can be provided when white phosphorus undergoes oxidation by tert-butyl hydroperoxide in the methanol solutions. When Cu (I), and Cn(Ⅱ) complexes are used as catalysts, it is possible to significantly enhance the oxidation of white phosphorus with the increase in the reaction rate. Th'e order for activity of catalysts is Cu(acac)2>Cu (CH3COO)2>Cu (C3 H7COO)2:>CuI>CuCI2. Noticeably, with Cu(acac)2 as catalyst, the selectivity for mono-alkyl phusphonates can reach 95% under adequate reaction conditions.
2.Molecular characterization of PrM/C and E genome of Japanese encephalitis virus isolate CQ11-66 from Chongqing
Lijuan XU ; Hua LING ; Sheng YE ; Yan FENG ; Chaomin ZHU
Chinese Journal of Microbiology and Immunology 2012;32(8):734-739
Objective To analyze the molecular characterization of PrM/C and E genome of Japanese encephalitis virus(JEV) strain,CQ11-66,a newly strain isolated from patients with epidemic encephalitis B Chongqing Municipal.Methods The samples were collected from Children's Hospital of Chongqing Medical University,and inoculating BHK-21 cells were used to detect and isolate the Japanese encephalitis virus(JEV) strain,computer analysis of the phylogenetic,nucleic acid data and deduced amino acid sequence was accomplished using the Clustal X(1.8) and MEGA5 programs.Results Only one JEV strain was isolated from patient's cerebrospinal fluid specimen,named CQ11-66.Comparison of the PrM/C genome sequence of strain CQ11-66 with other 31 JEV isolates showed a 74.8%-97.4% nucleotide sequence homology among them,which resulted in 85.6%-98.7% amino acid sequence homology; Meanwhile,comparison of the E genome sequence of strain CQ11-66 with other 35 JEV isolates showed a 81.6%-99.6% nucleotide sequence homology among them,which resulted in 94.8%-99.6% amino acid sequence homology.There were high homology between CQ11-66 and JEV isolates from Fujian province on nucleotide sequence and amino acid sequence.Phylogenetic analysis of PrM/C and E genome showed that the CQ11-66 belonged to genotype Ⅲ.Conclusion Only one JEV strain was isolated from patient's cerebrospinal fluid specimen.There were some differences between CQ11-66 strain and other JEV isolates,and CQ11-66 strain belonged to genotype Ⅲ.
3.Use of allograft vessels for portal venous reconstruction during radical resection for pancreatic cancer
Weilin WANG ; Song YE ; Yan SHEN ; Min ZHANG ; Sheng YAN ; Shusen ZHENG
Chinese Journal of General Surgery 2016;31(5):365-369
Objective To explore the clinical usage of allograft vessels for portal venous reconstruction during radical resection for pancreatic cancer.Methods We retrospectively analyzed clinical data of 14 patients who underwent pancreaticoduodenectomy with vascular resection (PDVR) and reconstruction by allograft vascular form June 2009 to May 2013.Results Patient's mean age was (59.1 ±10.4) years old.The mean diameter of tumors was (3.9 ± 1.3) cm,mean lcngth of resected vessels was (4.4 ±0.5)cm,and the R0 resection rate was 71.4%.The implanted vessels in 4 cases were allograft portal veins,and in the other 10 cases were allograft iliac arteries.In morphological classification,9 cases used straight vessels and 5 cases used Y shaped vessels.The overall median survival time of the group was (14.0 ± 3.0) months.The 1,2 year' s survival rate was 52.0% and 40.0%,respectively.Conclusions Allograft vessels for portal venous reconstruction during PDVR have biological and structural advantages.
4.Not Available.
Feng ZHANG ; Yan yan FAN ; Guang hua YE ; Xing biao LI ; Lin sheng YU ; Zhong ZHANG
Journal of Forensic Medicine 2021;37(5):727-728
5.Effects of Hypericum perforatum L. extract on anti-stress response. In normal mice and antioxidant activity in exhaustive exercise mice.
Ting-Ting YE ; Le LI ; Ying-Ying LIAO ; Ji-Zhong YAN ; Sheng-Qiang TONG
Chinese Journal of Applied Physiology 2014;30(4):332-334
Animals
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Antioxidants
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metabolism
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Fatigue
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blood
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Female
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Hypericum
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chemistry
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Male
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Mice
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Mice, Inbred ICR
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Physical Conditioning, Animal
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Plant Extracts
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pharmacology
6.The experimental study on infant rabbit lung injury induced by ischemia-reperfusion
Wanshan QIU ; Bing JIA ; Ming YE ; Xiangang YAN ; Gang CHEN ; Qilin TAO ; Sheng SHEN ; Zhanggen CHEN
Chinese Journal of Thoracic and Cardiovascular Surgery 2012;(12):729-731
Objective To explore the characteristics of ischemia-reperfusion induced infant lung damage and the potential mechanisms of the injuried.Methods Both infant (15-21 days old) and adult (5-6 months old) rabbits were subjected to either ischemia-reperfusion or sham operation.Ischemia-reperfusion was induced by clamping the right pulmonary hilum for 1 hour and then removal of the clamp for 4 hours under anesthesia.The lung tissue were sampled for histological examination by light and electron microcopies and for biological evaluation of mitochondrial alterations.Production and expression of free radical species-hydroxyl radical (ROS-HR),malondialdehyde (MDA),superoxide dismutase (SOD),glutathione peroxidase (GSH-PX),myeloid differentiation factor-88 (MyD-88),and nuclear factor-κB (NF-κB) in the lung tissue were also examined.In addition,circulating levels of interleukin-β and tumor necrosis factor-α were measured during the ischemia-reperfusion process.Results In comparison to adult lungs,the infant lungs had more increased neutrophil infiltration,edema,swelled alveolar epithelial and endothelial cells,and severer mitochondrial impairment reflected by damage of the inner membrane as well as decrease in the membrane potential after ischemia-reperfusion.The lungs in infant animals subjected to sham operation displayed higher levels of ROS-HR and MDA and lower levels of SOD and GSH-PX than those in adult controls.The lungs in infants with ischemia-reperfusion were found to further produce more ROS-HR,and MDA,and less SOD and GSH-PX than the ischemia-reperfused adult lungs.Moreover,the circulating levels of interleukin-1β and tumor necrosis factor-α were elevated during the period of ischemia-reperfusion,particularly in the infant animals,which appeared to be associated with the expression of MyD-88 and NF-κB in the lungs.Conclusion Lung ischemia-reperfusion causes more severe lung damage in infants than in adults,probably due to combination of low antioxidant capacity and overproduction of ROS in infants.
7.Study on the killer cell immunoglobulin-like receptor gene polymorphisms in leukemia patients and their siblings who have same HLA-A/B/DR typing
Yan ZHANG ; Sheng LIU ; Mengli LIU ; Shihui YE ; Bo WANG ; Chunmei SHEN ; Jun QI
Chinese Journal of Microbiology and Immunology 2008;28(10):895-898
Objective To investigate the relationship between killer cell immunoglobulin-like receptors(KIR)and HLA by distribution of KIR gene in leukemia patients and their siblings who have same HLA-A/B/DR typing.Methods KIR genotypes were detected by PCR-SSP on 78 patients and their siblings who have same HLA-A/B/DR typing.Results There were 48.72%in 78 patients who had same KIR genotypes with their siblings while the 44.87%patients had different KIR genotypes with their siblings.There was no difference in frequency between patients and their siblings(P>0.05).There were no differences in frequency among chronic myelocytic leukemia(CML),non acute lymphoblagtic leukemia(NALL)and acute lymphoblagtie leukemia(ALL)but the frequency of KIR2DS4 in CML was higher than others.Condusion The KIR gene and HLAⅠ antigen are heredity independently and relatively stable.The factor of disease has little effect on KIR gene.
8.Chemical constituents from twigs of Euonymus alatus.
Lei ZHANG ; Yan ZOU ; Xian-sheng YE ; Jia ZHANG ; Wei-ku ZHANG ; Ping LI
China Journal of Chinese Materia Medica 2015;40(13):2612-2616
To investigate the chemical compounds from the twigs of Euonymus alatus, nine compounds were isolated and identified as(+)-delta(2,11)-enaminousnic acid(1), 11-keto-beta-boswellic acid(2), acetyl 11-keto-beta-boswellic acid(3), camaldulenic acid(4), betulinic acid(5), 6beta-hydroxy-stigmast-4-en-3-one(6), 5-hydroxy-6,7-dimethoxyflavone(7), ethyl 2,4-dihydroxy-6-methylbenzoate(8), 4,4'-dimethoxy-1,1'-biphenyl(9). Their structures were elucidated by extensive spectroscopic analysis. Among them, compound 1 was a new natural product. Compounds 2-4 and 7-9 were obtained from the Euonymus genus for the first time. In vitro study showed that compounds 2 and 3 showed significant anti-tumor activities to BEL-7402 and HCT-8 at the concentration of 10 mg x L(-1). The inhibition rate of compound 2 was 61.78% and 68.29%, whereas the inhibition rate of compound 3 had reached to 70.91% and 84.07%.
Antineoplastic Agents, Phytogenic
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chemistry
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isolation & purification
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pharmacology
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Cell Line, Tumor
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Euonymus
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chemistry
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Humans
9.Chondrosarcoma of kidney: report of a case.
Xiao-ye ZHANG ; Yan WANG ; Geng-yin ZHOU ; Jing GAO ; Wei-sheng XU
Chinese Journal of Pathology 2010;39(9):637-637
Aged
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Carcinoma, Renal Cell
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pathology
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Carcinosarcoma
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pathology
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Chondroma
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pathology
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Chondrosarcoma
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complications
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metabolism
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pathology
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surgery
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Diagnosis, Differential
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Female
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Humans
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Kidney Neoplasms
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complications
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metabolism
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pathology
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secondary
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surgery
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Lung Neoplasms
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secondary
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Nephrectomy
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Pleural Effusion, Malignant
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etiology
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S100 Proteins
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metabolism
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Soft Tissue Neoplasms
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pathology
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Vimentin
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metabolism
10.The effect of reduced glutathione and venous systemic oxygen perfusion on apoptosis and ultrastructure of rat steatotic liver grafts
Sheng YE ; Benli HAN ; Jiahong DONG ; Jin ZHU ; Kun LI ; Yan XIONG
Chinese Journal of General Surgery 1993;0(02):-
Objective To explore the effect of reduced glutathione and venous systemic oxygen perfusion on apoptosis and ultrastructure of hepatocytes in rat steatotic liver grafts. Methods Before liver transplantation grade Ⅱ steatotic liver model was established by a diet consisting of 79% standard diet,20% lard and 1% cholesterol for 6 weeks. In pretreatment group, the donor received intraperitoneal injection of reduced glutathione at a dosage of 500 mg/kg/body weight 3 times a day for 2 days, and intrahepatic venous oxygen perfusion for 6 hours while kept in cold preservation. Results Preconditioning measures in steatotic liver grafts significantly decreased the hepatocytes necrosis (38?10)% vs (17?6)%, P