Objective To investigate the effect of triiodothyronine on the expression of alpha subunit of C protein Co (Coa) gene in cultured rat cerebral cortex neurons. Methods Primary cultured neurons of rat were prepared from the cerebral cortex at embryonic day 19. The neurons were cultured in DMEM supplemented with 15% fetal calf serum. After 7 days, the neurons were cultured in different media; DMEM supplemented with 15% fetal calf serum (group A), DMEM supplemented with 15% fetal calf serum stripped of thyroid hormone (group B), 0.5nmol/L T3-containing DMEM supplemented with 15% fetal calf serum stripped of thyroid hormone (group C) , 5nmol/L T3-containing DMEM supplemented with 15% fetal calf serum stripped of thyroid hormone (group D) and 50nmol/L T3-containing DMEM supplemented with 15% fetal calf serum stripped of thyroid hormone (group E). The neurons were cultured for another 7 days and total RNA was extracted. Goa mRNA leves were measured by competitive RT-PCR. Results As compared with group A, Coa mRNA leves in group B, D and E were low (P 0.05). Conclusion T, has dual effects on the expression of Coa gene in cultured rat cerebral cortex neurons. T3 down-regulated Coa mRNA in cultured neuron. However, in the absence of T3, Goa mRNA was also very low. Thyroid hormone may exert their action on brain development by regulating the the expression of Goa gene.

Objective To assess the feasibility of low voltage with CARE dose 4D computed tomography pul-monary angiography(CTPA)by using image quality and radiation dosage.Methods 92 patients with clinically sus-pected pulmonary embolism(BMI:20 ~30kg/m2)were randomly divided into two groups(group A:n =46,80kV;groups B:n =46,100kV),the male and female of group A were equal to the number.Image quality score(given blind-ly by two senior radiologists ranged from 1 to 5 points).The CT value and standard deviation(SD)were measured and recorded in common pulmonary artery trunk,the main right pulmonary arteries,right low lobar arteries,and erector muscle of spine,calculated the SNR of ROI.Average CT value,signal noise ratio(SNR),CTDIvol,DLP were com-pared between the two groups.Used the CT dose index(CTDIvol)to compare radiation dosage between group A female and male.Results The agent average CT value and SD in arteries in group A [(510.27 ±115.45)HU,(50.06 ± 11.67)HU respectively]were higher than those in group B[(413.32 ±100.38)HU,(35.12 ±11.94)HU respective-ly],there were significant differences between the two groups(t =10.367,8.892,all P =0.000),but the SNR of ROI was (12.36 ±3.90),which was lower than (14.03 ±4.46)in group B,there was significant difference between the two groups(t =-5.238,P =0.001 ).Image quality score of transverse ection(1mm)in group A was (4.20 ± 0.29),which was lower than (4.91 ±0.36)in group B,there was significant difference(t =-20.23,P =0.000), but there was no significant difference in coronal section(5mm),coronal maximum intensity project(MIP)image and the capability of displaying the pulmonary artery branches(all P >0.05 ).The CTDIvol in group A was (2.03 ± 0.39)mGy,which was greatly lower than (5.04 ±1.02)mGy in group B,there was significant difference (P <0.05);but there was no significant difference between female and male in group A[male:(2.05 ±0.39)mGy;female:(2.01 ±0.38)mGy,t =-0.300,P >0.05].Conclusion Radiation dose can be decreased greatly(above 50%)in 80kV CTPA compared 100kV without compressing obviously the image quality for patients BMI 20 -30kg/m2 ,and no significant difference between the group A(80kV)different gender radiation dosage.

Objective To evaluate the advantage of single -source spectrum CT in differentiating of calcifi-cation and tophus.Methods 146 cases of clinical suspected patients with gout pain were scaned by single -source spectrum CT,observed the adjacent to the bone and joint hyperplasia and showing characteristics of gout stone,coun-ted quantity,size and position of the sedimentary tophus.Diagnosed by American rheumatism association standard as the gold standard,computed the sensitivity,specific degrees and accuracy of showing tophus of single -source spec-trum CT and conventional CT,and evaluated the advantage of differentiating adjacent to the bone and joint hyperplasia and gout stone of single -source spectrum CT.Results Diagnosis of gout in 105 cases.Scaned 349 joints,positive in 341 of them,The first plantar toe joint involvement was obvious[118,34.60%(118/341)];The result showed that the sensitivities of the tophus,specific and accurate rate of the conventional CT were 63.80%(67 /105),65.85%(27 /41),64.38%(94 /146),which of single -source spectrum CT were 96.19%(101 /105),87.80%(36 /41), 93.84%(137/146),the differences of sensitivity,specificity and accuracy between the two methods were statistical significance(χ2 =34.42,555,38.34,all P <0.05).Conclusion Single -source spectrum CT in gout stone adjacent to the bone and joint has higher sensitivity and accuracy,specific degree,it is higher than conventional CT and provide a new noninvasive imaging technique for the identification of the peripheral bone and joint of the bone.

Objective To identify the correlativity between SCN4A V781I mutation and hypokalemic periodic paralysis(HOKPP).Methods The SCN4A V781I mutation was screened on the members of a hypokalemic periodic paralysis family(including 3 patients and 14 healthy relatives), 71 sporadical hypokalemic periodic paralysis patients and 100 healthy adults with the PCR sequencing and the incision enzyme techniques.Results The mutation existed not only in all the patients but also in 4 healthy relatives, including 1 male and 3 females, in the hypokalemic periodic paralysis family.Seven of 71 sporadieal hypokalemic periodic paralysis patients and 7 of 100 health adults indicated the SCN4A V781I mutation.There was no significant difference in the SCN4A V781I mutation rate between the hypokalemic periodic paralysis patients and the healthy adults(X~2=0.452, P=0.502).Conclusions SCN4A V781I mutation maybe is an innocent polymorphism.There is little correlativity between SCN4A V781I and hypokalemic periodic paralysis.

0.05).Meanwhile,the cardiac function and the quality of life of the rehabilitation exercise group was improved to a significantly larger extend than those of the control group (P

OBJECTIVE:To investigate clinical efficacy and safety of goserelin in the treatment of adenomyosis complicated with severe dysmenorrheal before placing levonorgestrel intrauterine system (LIS). METHODS:A total of 140 adenomyosis pa-tients with severe dysmenorrheal were selected from Sanya Family Planning Technology Service Center during Jan. 2014-Jan. 2016, and then divided into control group and observation group according to lottery method,with 70 cases in each group. Both groups re-ceived LIS within first 7 d of menstruation,and observation group was given Goserelin acetate sustained-release implant 10.8 mg subcutaneously via anterior abdominal wall one week before placing. Therapeutic efficacy was evaluated 6 months later. The im-provement of dysmenorrhea was compared between 2 groups. The uterine volume,COX dysmenorrhea score (CMSS),menstrual volume,hemoglobin (Hb) and CA125 content were compared between 2 groups before and after treatment. The occurrence of ADR was also compared. RESULTS:The rate of dysmenorrheal improvement in observation group was 95.71％,which was signifi-cantly higher than 81.43％of control group,with statistical significance(P<0.05). Before treatment,there was no statistical signif-icance in above indexes between 2 groups (P>0.05). Compare to before treatment,CMSS score,menstrual volume and CA125 content of 2 groups were decreased significantly after treatment,while Hb content was increased significantly;the observation group was significantly better than the control group,with statistical significance(P<0.05). There was no statistical significance in uterine volume between 2 groups before and after treatment (P>0.05). There was no statistical significance in the incidence of ADR(10.00％ vs. 14.29％)between 2 groups(P>0.05). CONCLUSIONS:Goserelin in the treatment of adenomyosis complicated with severe dysmenorrheal before placing LIS can effectively relieve dysmenorrheal,reduce menstrual volume and improve Hb and CA125 levels,but do not increase the risk of ADR.

Antigens, CD34 ; metabolism ; Cytokines ; metabolism ; Diagnosis, Differential ; Humans ; Immunohistochemistry ; Male ; Middle Aged ; Mucin-1 ; metabolism ; Perineum ; pathology ; Phosphopyruvate Hydratase ; metabolism ; Sarcoma ; metabolism ; pathology ; surgery ; Vimentin ; metabolism

Objective To investigate the differences of regional homogeneity(ReHo) in depressed patients with vary degrees of anxiety using resting-state function MRI,and the relationship vary degrees of anxiety symptoms.Methods 10 depressed patients with scores less than 1 in anxiety/somatization factor of Hamilton depression scale,10 depressed patients with scores above 2,and 10 age-,gender-,education-matched healthy controls were underwent resting-state fMRI scanning at 3.0 Tesla.Correlation analysis between ReHo with significant differences level of brain region and the total score of Hamilton depression scale,and anxiety/somatization factor were underwent respectively.Results The brain regions with significant difference level among three groups were the left posterior cerebellum(-39,-77,-29 ; K =15),the left angular gyrus (-56,-63,22 ; K =13) and the left anterior cingulate (-3,22,29 ; K =19) (P ＜ 0.05,Alphasim) ; compared with healthy group,the mild anxiety group showed significantly decreased ReHo in the left posterior cerebellum,and increased ReHo in the left anterior cingulate; the moderate and severe anxiety group showed significantly decreased ReHo in the left posterior cerebellum,and increased ReHo in the left anterior cingulate and the left angular gyrus; no significant differences were found in patient groups.In the mild anxiety group,the left anterior cingulate positively correlated with the anxiety severity(r=0.646,P=0.043),and in the severe anxiety group positively correlated with the somatic anxiety(r =0.693,P=0.038).Conclusion Depressed patients showed abnormal neural activity in resting state,and the degree of abnormality is associated with clinical anxiety/somatization symptoms.

Objective To evaluate the diagnostic and prognostic value of BRAF V600E mutation screening of ultrasound-guided fine-needle aspiration (FNA)specimens in patients with thyroid nodule. Methods The BRAF V600E mutation status were assessed in FNA specimens of 104 patients with thyroid nodules before operations.The BRAF mutation status,clinical,and pathology records of the patients were reviewed and the associations between these characteristics and papillary thyroid cancer (PTC ) were analyzed.Results Seventy-one PTC and 14 benign thyroid nodules were included in this study.BRAF V600E mutations were found in 57/71 (80%)PTC.All benign thyroid nodules had no BRAF V600E mutation.The sensitivity,specificity,positive predictive value and negative predictive value of BRAF V600E mutations in differentiation between PTC and benign thyroid nodules were 80%,100%,100% and 50%(P < 0.001 ).In 44 patients with PTC who underwent surgery,the central compartment lymph node metastases and extrathyroidal invasion were not significantly different between BRAF-positive and BRAF-negative PTC (P = 0.283 and 0.307 ).Conclusions BRAF V600E mutation may be a potential tool to facilitate ultrasound in diagnosis of PTC.In patients with PTC,the presence of the BRAFV600E mutation was not significantly associated with prognostic factors.

Objective To study the safety and efficacy of transhepatic arterial infusion embolization using microspheres combined with lipiodol for treatment of hepatocellular carcinoma(HCC).Methods Transhepatic arterial chemoembolization(TACE)was performed with 1-2 ml 300-500?m mierospheres and 10-20 ml lipiodol for treatment of HCC.Changes of liver function,AFP,responses of tumor and complications related to embolization were analyzed before and after TACE.Results Thirty six patients with HCC were enrolled into this study.After TACE,patients' liver function got worsen and tumor size decreased significantly(P＜0.05).Conclusion TACE with use of microspheres combined with lipiodol is an effective treatment for HCC,but should be taken carefully to avoid hepatic failure.