1.Research on the Method of Quantitative Assessment for Walking Function
Chinese Journal of Rehabilitation Theory and Practice 1996;2(4):154-158
In this paper a series of gait quantitative assessment indexes:phase symmetry index,verti-cal force symmetry index,equilibrium function index,and brake and drive function indexes are obtainedwith VICON 3-dimensinal movement analysis system according to the results that 40 amputees,50 cerebralpalsy patients, 31 hemiplegia patients and 19 paraplegia are evaluated and the differences between the nor-mal and the abnormal(p<0.001)found with these indexes. The quantitative assessment methed of walk-ing function not only provides rehabilitation level of patients and for checking therapeutical effects,but alsolaies the foundation of gait analysis to standardization and practicalization further.
2.Comparative analysis of Han and Uygur college students emmetropia biology measurement
Meng, LIU ; Yan, WANG ; Ning, GUO
International Eye Science 2014;(10):1846-1848
AIM: To observe the differences in central cornea thickness, anterior chamber depth, corneal anterior curvature and corneal posterior curvature between the Uygur and the Han nationality college emmetropia students, and analyze the relationship of corneal thickness and corneal curvature.
METHODS: More than 500 students in grade one in Xinjiang Medical University were underwent screening, 56 emmetropia eyes in Han nationality students and 51 in Uygur students were selected. Sirius corneal topography was applied to the measurement.
RESULTS: Uygur and the Han nationality college emmetropia students central cornea thickness ( Uygur:0.53±0. 03mm, Han: 0. 54±0. 03mm), anterior chamber depth ( Uygur: 2. 91±0. 26mm, Han: 3. 14±0. 25mm), corneal posterior curvature K2 ( Uygur: -6. 30±0. 23D, Han: -6. 38±0. 28D) had significant difference (P<0. 05). Corneal anterior curvature ( K1, K2 ) before and corneal posterior curvature K1 there was no significant difference (P>0. 05). Han had no significant relationship in corneal thickness and corneal anterior curvature ( r<0 ) , or in corneal posterior curvature ( r < 1 ) . Uygur has no significant relationship in corneal thickness and corneal anterior curvature (r<0), or in corneal posterior curvature (r<1).
CONCLUSION: There are significant differences between the Uygur and the Han nationality college emmetropia students in cornea thickness, anterior chamber depth and corneal posterior curvature K2. There is no significant relationship between corneal thickness and corneal curvature in Uygur and the Han nationality college students.
4.Analysis of related risk factors and control measures of senium patients with type 2 diabetes
Huawei TIAN ; Shengyuan LIU ; Nan ZHENG ; Yan SUN ; Ning MA
Chinese Journal of Primary Medicine and Pharmacy 2013;20(19):2951-2952
Objective To explore the related risk factors and control measures for senium patients with type 2 diabetes and to provide basis for making control and prevention measures.Methods A retrospective case-control study was conducted.548 cases of type 2 diabetes patients in our hospital were selected as case group and other 640 cases of normal people who accepted physical examination in our hospital were selected as control group.Results Exposure rates of smoking,drinking,obesity,hypertension,hypedipidemia and family history in case group were obviously higher than those of control group(P <0.05).Conclusion Smoking,drinking,obesity,hypertension,hyperlipidemia and family history were risk factors of type 2 diabetes.Comprehensive intervention measures related to the risk factors,such as maintaining a good way life,were importantto prevent and control type 2 diabetes.
5.The effects of 17-β estradiol on hepatocyte apoptosis and expression of Bcl-2 and Bax in the liver-resected-rats with ischemia reperfusion injury
Fusheng LIN ; Shiqiang SHEN ; Ruicheng YAN ; Ning LI ; Jianchao LIU
Chinese Journal of Hepatobiliary Surgery 2012;18(3):215-219
Objective To investigate the effects of 17-β estradiol on hepatocyte apoptosis and the expression of Bcl-2 and Bax in hepatic tissue after reduced-size ischemia reperfusion injury and its mechanism in liver protection.Methods A rat model of reduced-size hepatic ischemia-reperfusion injury was established in 75 male Sprague-Dawley rats.They were randomly allocated into three groups:Sham group,ischemia-reperfusion(IR)group,and 17-β estradiol(E2 + IR)group.Liver functions,liver histology and hepatocellular apoptosis rates were observed after reperfusion.Hepatocellular ap optosis was determined by terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL)method and the expression of Bcl-2 and Bax were determined by Western blotting.Results The levels of ALT and AST were higher and peaked after 12 h of reperfusion in the IR group compared with the sham group.The histological changes in the liver of the IR group consisted of hepatocyte swelling,hepatic sinusoids narrowing,inflammatory cell infiltration and hepatocyte necrosis in some areas of the livers.The IR group also exhibited an increased rate of hepatocellular apoptosis at 12 h after reperfusion.The protein expression of Bcl-2 decreased while the expression of Bax increased.In the 17-β estradiol group,the levels of ALT and AST were lower,the pathological changes were milder and the rate of hepatocellular apoptosis was lower at 12 h in comparison to those of the IR group.The expression of Bcl-2 was higher and the expression of Bax was lower in the 17-β estradiol group in comparison to those of the IR group.Conclusions 17-β estradiol can relieve the hepatic ischemia reperfusion injury in rat livers.17-β estradiol may inhibit apoptosis in hepatic tissue by up regulating Bcl-2 and down-regulating Bax,thus producing a protective effect on hepatic ischemia-reperfusion injury.
6.Interventional treatment of arteriovenous malformation of hands
Jianguo YIN ; Xiaohong LIU ; Yan YANG ; Jijun NING ; Xiong JIA
Journal of Interventional Radiology 1994;0(04):-
Objective To review and summarize the effects of interventional treatment of hand arteriovenous malformation in 5 cases.Methods Through catheterization of brachial artery ipsilaterally the blood-supply artery of arteriovenous malformation was embolized,using high-temperaure managed gelatin sponge,silk thread,pingyangmycine and spring coil.Results Total 13 blood-supply arteries in 5 cases were embolized with successful rate of 100%,including 1 case of complex malformation under effectively controlled,3 cases cured clinically and 1 case still awaiting observation.Conclusions Transcatheter arterial embolization for the treatment of hand arteriovenous malformation is safe,effective and high successful.(J Intervent Radiol,2007,16:277-279)
7.Clinical Manifestations and Imaging Diagnostic Value of Tuberous Sclerosis Accompanied by Multiple Organs Injury
Jianguo YIN ; Xiaohong LIU ; Jijun NING ; Yan YANG ; Xiong JIA
Journal of Practical Radiology 1996;0(04):-
Objective To discuss the clinical manifestations and imaging diagnostic value of tuberous sclerosis accompanied by multi-organ injuries.Methods 2 cases in our hospital and 27 cases of tuberous sclerosis from the published reports were collected.The clinical manifestations and imaging features as well as the extent and type of multi-organ lesion were analyzed.Results Among the 29 patients,17 were male and 12 were female.The averaged age was 16.8.The clinical manifestations included:with family history in 6 , epilepsies in 25 (15 with mental retardation),symmetric angiofibroma in the face in 22 (3 with similar changes in the else body surface),fibromas beneath toenail or fingernail in 4, hyperdactylia of thumb in 2 and abdominal masses in 7 (4 with hematuria), tuberous scleroses beneath ependymal layer in 27,17 patients with kidney diseases,9 involved liver,5 involved spleen.Conclusion Multiple organs can be involved by tuberous sclerosis.The calcification of ependymal lyer and harmatoma of kidney are the most valuable in imaging diagnosis of the disease.
8.Effect of Al₂O₃ sandblasting on the bond strength between 3mol% yttrium-stabilized tetragonal zirconium polycrystal zirconia framework and veneering porcelain.
Qiang ZENG ; Ning LI ; Yanan ZHOU ; Jiazhen YAN ; Wenbo LIU
West China Journal of Stomatology 2015;33(6):638-641
OBJECTIVEThe effect of sandblasting on the bond strength between 3mol% yttrium-stabilized tetragonal zirconium polycrystal (3Y-TZP) zirconia framework and veneering porcelain was evaluated.
METHODSA total of 21 specimens [(25 ± 1) mm x (3 ± 0.1) mmx (0.5 ± 0.05) mm] were prepared according to ISO 9693. The specimens were then randomly divided into 3 groups. Sandblasting was performed on 2 meshes of Al₂O₃ particles: group A with mesh 110 and group B with mesh 80. Group C, which was not sandblasted, was the control group. The surface roughness of the zirconia framework, as well as the bond strength between 3Y-TZP zirconia framework and veneering porcelain, was measured. The interface microstructure was observed by scanning electron microscope (SEM), and elemental distribution was detected by energy dispersive spectroscopy (EDS).
RESULTSSurface roughness values were (1.272 ± 0.149) μm for group A, (0.622 ± 0.113) μm for group B, and (0.221 ± 0.065) μm for group C. Statistical significance were found among groups (P < 0.05). The bond strength values were (28.21 ± 1.52) MPa for group A, (27.71 ± 1.27) MPa for group B, and (24.87 ± 3.84) MPa for group C. Statistical significance was found between group A and group C (P < 0.05), whereas the other groups had no statistical significance (P > 0.05). Interface adhesion failure was the primary performance. SEM images showed the close interface bonding, and EDS showed that the interface had no obvious element penetration.
CONCLUSIONAl₂O₃ sandblasting can slightly enhance the bond strength between zirconia framework and veneering porcelain.
Aluminum Oxide ; chemistry ; Dental Bonding ; Dental Porcelain ; chemistry ; Dental Stress Analysis ; Dental Veneers ; Materials Testing ; Microscopy, Electron, Scanning ; Shear Strength ; Surface Properties ; Yttrium ; chemistry ; Zirconium ; chemistry
10.Cloning,expression and purification of fragile X mental retardation protein
Jian LIU ; Ke ZOU ; Ning ZHU ; Yan SHEN
Basic & Clinical Medicine 2006;0(07):-
Objective Explore the conditions of the cloning,expression and purification of FMRP.Methods The plasmid pET22b(+)-FMR1,constructed by molecular cloning,was transformed into E.coli BL21(DE3) competent cells and induced to express FMRP by IPTG.Recombinant FMRP was purified by affinity chromatography,verified by Western-blot,and tested for its RNA binding ability.Results FMR1 cDNA was successfully cloned into pET22b(+) vector and expressed in E.coli BL21(DE3).A protein with Mr 79 000 was purified and confirmed to be FMRP.This protein retained the RNA binding ability of FMRP.Conclusion We successfully expressed recombinant hFMRP with high purity and activity in E.coli,which provided a reliable material to study the function of FMRP.