Objective To study immunologic mechanism of tiger's urine on patients of rheumatoid arthritis. Method We used ELISA method to detect TNF values of serum; we also used MTT colorimetric method to detect the inhibition of tiger's urine on T－ cell. Result TNF values of RA patient's serums at both active stage and stationary phase before the therapy were higher than those of healthy group; TNF values of patient's serums after the therapy were lower than those before the therapy; tiger's urine had obvious inhibitive action on proliferation of peripheral blood's T－ cell. Conclusion Tiger's urine could inhibit T－ cell's proliferation and TNF's secretion, which may be the important mechanism of curing RA.

OBJECTIVE:To provide the basis for the formulation of the health intervention strategy for medical and health insti-tutions'pharmacists and provide reference for other related research. METHODS:A total of 180 medical and health institutions' pharmacists in Heilongjiang province were investigated and evaluated by work satisfaction MMSS scale(modified)and quality of life(SF-36)scale,and the relevant data were analyzed statistically. RESULTS:A total of 180 questionnaires were distributed and 134 valid questionnaires were collected. The effective recovery rate was 74.44%. The average score of work satisfaction degree was (3.44±0.79),which was at the middle level. The highest score was the relationship with colleagues and the lowest score was wel-fare. The physical function,the physiological function and the social function dimension of the respondents were higher thanChi-nese norm. The scores of physical pain,general health,vitality,emotional function and mental health were lower thanChinese norm,and there was statistical significance between the scores of the other dimensions andChinese norm(P<0.05),except for the physiological function and vitality dimension. Multivariate analysis showed that gender,duties,education,length of service and work satisfaction score had an effect on quality of life(P<0.05),among these factors,work satisfaction score was positively correlated with quality of life. CONCLUSIONS:The quality of life of medical and health institutions'pharmacists still needs to be improved,which is related to many influential factors,and job satisfaction is an important influential factor.

Aim: To investigate the functional influences of saponins from Anemarrhena asphdeloids Bge (SAaB) on lipopolysaccharide (LPS)-induced RAW264. 7 cells and the regulation of SAaB to NF-κB- iNOS-NO signaling pathway. Methods: The inflammatory cell model in vitro was established using LPS- induced RAW264. 7 cells, and the levels of NO, iN- OS, TNF-α and IL-6 in inflammatory RAW264. 7 cells effected by SAaB were analyzed using Griess and ELISA method respectively. The protein expression levels of NF-κB p65 were measured by Western blot. Results: Compared with control group, LPS (10 mg L-1) -induced RAW264. 7 cells expressed a significant increase in the levels of NO, iNOS, TNF-α, IL-6 and NF-κB p65 protein. SAaB (0.3, 3, 30 mg L-1) obviously reduced the contents of these inflammatory factors (P <0. 01) and the expression of NF-κB p65 protein (P < 0. 01) in LPS-induced RAW264. 7 cells. Conclusion: SAaB can inhibit the functions of LPS-induced RAW264. 7 cells by modulating NF-KB- iNOS-NO signaling pathway.

Objective To analyze the long-term prognosis and prognostic factors of idiopathic collapsing focal segmental glomerulosclerosis (FSGS) and not otherwise specified FSGS in children. Methods The clinical, pathology and follow-up data of patients with idiopathic collapsing FSGS and not otherwise specified FSGS were analyzed retrospectively by Kaplan-Meier method, univariate and multivariate Cox regression analysis. Results A total of 64 patients (29 idiopathic collapsing FSGS and 35 not otherwise specified FSGS) were diagnosed by renal biopsy. The 4-year renal survival rate of idiopathic collapsing FSGS and not otherwise specified FSGS were 48.3%, 74.3% respectively. Univariate analysis revealed that the renal survival time were 25.41±3.28 months in idiopathic collapsing patients, and 35.53±2.73 months in not otherwise specified patients. The different is significant (χ2=4.07,P=0.044). Multivariate Cox regression analysis showed that poor treatment response (HR=5.92, P<0.05) and renal insufficiency at early stage (HR=2.45, P<0.05) were independent risk factors of prognosis. Conclusions Compared with patients with not otherwise specified FSGS, the renal survival time is shorter in idiopathic collapsing FSGS patients. Patients with renal insufficiency and poor response to treatment have poorer prognosis.

To study the effect of miR-490 on Coxsackievirus B3 (CVB3) replication, HeLa cells were trans- fected with miR-490 in vitro, and infected with a Renilla luciferase (RLuc)-expressing CVB3 variant (RLuc-CVB3). The activities of RLuc in these cells were measured at 8h intervals from 0 to 40 h post-infection (p.i.), and the effects of miR-490 on RLuc-CVB3 replication were observed. In a further study, HeLa cells were transfected with either miR-490 or antisense miR-490 (AMO-miR-490), and were then infected with an enhanced green fluorescence protein (EGFP)-expressing CVB3 variant (EGFP-CVB3). The replication of EGFP-CVB3 was then determined by detecting the expression of EGFP. We observed that miR-490 could significantly inhibit the expression of RLuc in infected cells at 32 h p. i. Furthermore, in HeLa cells infected with EGFP-CVB3 at 32 h p.i., EGFP expression was also significantly inhibited by the presence of mniR-490. The inhibitory effect of miR-490 on EGFP expression in EGFP-CVB3-infected cells could be reversed by tranfection with AMQ-miR-490. These results indicated that miR-490 significantly inhibits the replication and expression of QVB3.
Enterovirus B, Human ; genetics ; physiology ; Enterovirus Infections ; genetics ; metabolism ; virology ; HeLa Cells ; Humans ; MicroRNAs ; genetics ; metabolism ; Virus Replication

Point "Siguan" is seen in the Miraculous Pivot: Jiuzhen Shieryuan for the first time. From then on the doctors of every dynasties cited point "Siguan" and explained its content. But the authors hold that their explanations do not conform with the meaning in the Huangdi's Internal Classic. Therefore, the authors put forward their explanations for point "Siguan" and indicate that correct understanding of point "Siguan" is very important for treatment of diseases of five-zang organs.
Acupuncture Points ; Acupuncture Therapy ; Humans ; Medicine, Chinese Traditional ; Meridians

High price and poor stability of both crocin-1 and crocin-2 reference substance have become obstacles to HPLC assay of Croci Stigma. A new method based on reference extract was proposed. In this study, the reference extract was prepared from gardenia yellow which is cheap and easy to get The content of crocin-1 and crocin-2 in reference extract was determined and factors affecting stability of reference extract were investigated. Twelve batches of Croci Stigma were analyzed with reference extract and reference substance respectively. The results showed no difference. The presented method is feasible for quality control of Croci Stigma and reference extract is suitable to replace reference substances in assay.
Carotenoids ; analysis ; Chromatography, High Pressure Liquid ; standards ; Crocus ; chemistry ; Drugs, Chinese Herbal ; analysis ; Quality Control ; Reference Standards

Adult ; Diagnosis, Differential ; Endometriosis ; pathology ; surgery ; Female ; Follow-Up Studies ; Humans ; Intervertebral Disc Displacement ; pathology ; Lumbar Vertebrae ; pathology ; Magnetic Resonance Imaging ; Spinal Canal ; pathology ; surgery ; Spinal Diseases ; pathology ; surgery

OBJECTIVECongenital esophageal stenosis owing to tracheobronchial remnants (TBR) is a rare condition. This study was conducted to understand the clinical features of TBR.

METHODThe data of the four cases with TBR admitted to our hospital and 76 patients identified from the literature were reviewed. The clinical manifestation, X-ray, endoscopy, biopsy and treatment were studied retrospectively.

RESULTOf the total of 80 cases, 45 were male, 33 were female, and for 2 cases the gender was unknown. Symptoms of dysphagia and regurgitation developed at the age of 1-day to 12-month. Definitive treatment was carried out at the age of 1-month to 16-year. Twenty-seven patients had associated anomalies with esophageal atresia being the most prevalent. X-ray esophagography showed segmental stenosis at the distal third of the esophagus in all patients except three. An abrupt narrow segment at the lower esophagus with marked proximal dilatation was found in 32 cases. Esophagography of 12 cases showed distal esophageal stenosis with tapered narrowing. Esophagography of 20 cases showed flask-shaped shadow of distal esophageal stenosis and one patient showed linear projection of barium at the level of stenosis. Endoscopy found almost complete obstruction of the lower esophageal lumen without signs of the esophagitis or reflux. Esophagoscopic dilatation of the stenosis was attempted in 24 cases, but was ineffective, and 3 patients suffered esophageal perforation. Seventy-nine patients underwent resection of the stenotic segment. Histologic examination of the resected specimen showed cartilage, mucus glands, resembling bronchal tissue. Post-operative complication included anastomotic stenosis, anastomotic leakage, hiatal hernia, and gastroesophageal reflux.

CONCLUSIONTBR should be suspected in patients who present with a typical history of dysphagia after ingestion of solid food. Esophagography and esophagoscopy are the essential means for diagnosis. TBR should be different from achalasia and should be diagnosed by biopsy. Operation is the only choice of treatment.

Anastomosis, Surgical ; Barium Sulfate ; Biopsy ; Child, Preschool ; Choristoma ; complications ; Dilatation ; Esophageal Atresia ; complications ; Esophageal Perforation ; etiology ; Esophageal Stenosis ; congenital ; diagnosis ; pathology ; surgery ; Esophagoscopy ; Female ; Follow-Up Studies ; Humans ; Infant ; Male ; Postoperative Complications ; epidemiology ; Retrospective Studies ; Tracheoesophageal Fistula ; etiology

The aims of microbiology experiment teaching are not only to cultivate the students’ capacity of basic operation,but also to expanse their knowledge scope.We applied the bacterial diversity on the teach-ing class to make the students understand the progress of microorganism genomics research.It is helpful to cultivate the students’ innovative spirit and ability.Easy to work,clear result and low cost facilitated the spread of this experiment in the university.