Objective To evaluate the value of immunoblotting in the detection of serum thymidine kinase 1 (TK1) in breast tumor patients. Methods TK1 in sera from breast tumor patients, non tumor patients and healthy volunteers were determined with immunoblotting assay.Results The levels of TK1 in breast cancer patients before operation, patients with benign breast tumor, non tumor patients, and healthy volunteers were (39.3? 33.9), (3.7?3.9), (1.7?0.5) and (1.4?0.5) pmol/L respectively. The value of TK1 was higher in malignant and benign breast neoplasms patients than in healthy volunteers. However, no significant difference in the TK1 level was found between breast cancer patients without metastasis after operation, non tumor patients, and healthy volunteers.Conclusions The immunoblotting of TK1 by anti TK1 antibody is a sensitive and specific assay. It can be used to monitor and predict the efficiency of the therapy as well as the breast neoplasms progression during clinical course.

Objective To investigate the clinical relationship between villus cells chromosome abnormality and spontaneous a-bortion in early pregnancy.Methods Villus cells from 112 women who just had spontaneous abortion in early pregnancy were cul-tured and prepared for karyotype analysis.Results 105 specimens were anaIyzed successfully which accounted for 93.75%(105/112).Among them 50 specimens were normal karyotype which accounted for 47.62%(50/105),55 cases were abnormal karyotype accounted for 52.38%(55/105).Abnormal number was the major type of abnormal karyotype.Among them there were 33 cases of trisomy which were most common,6 cases of triploid,1 case of tetraploid and 1 case of compound,2 cases of chimera,1 case of sex-ual chromatids and 6 cases of structural abnormalities.In the specimens with normal karyotype,20 cases were from male,30 cases were from female.Conclusion Embryo chromosome abnormality is one of the factors causing early pregnancy spontaneous abor-tion.Conduct villus chromosome examination in clinical treatment is useful to determine the reasons of abortion and to guide the next pregnancy.

A 44-year-old male complained recurrent swelling and pain in the left pre-auricular mass over 4 years, which aggravated for 4 months. Ultrasonography revealed a mixed mass echo in the left parotid gland. Computed tomography showed an enlarged parotid gland and a 1.5 cm x 0.9 cm low density shadow in the superficial lobe area with strip high-density shadow in the edge. Although the facial nerves of the patient were found adhered to the foreign body during the operation, the foreign body in parotid gland was removed surgically without any injure to them due to facial nerves protection. In conclusion, facial nerves dissection is necessary to avoid the postsurgical facial paralysis in the surgical removal of foreign bodies in parotid gland.
Adult ; Facial Nerve ; Foreign Bodies ; Humans ; Male ; Parotid Gland

Bone morphogenetic proteins (BMPs) are members of the transforming growth factor-β (TGF-β) superfamily, acting as important regulators in embryogenesis and bone and cartilage formation and repair.The complexity of BMPs action has early been unveiled by generous cell and molecular biology approaches and transgenic animal studies.Genetic engineering allows for the mass production of BMPs for clinical use of bone repair, but the desirable scaffold material is also needed to ensure controlled release and maximum biological activity of BMPs at the surgical site.Currently, BMP-2 and BMP-7 have been approved by United States Food and Drug Administration for clinical therapy in long bone fracture, spinal fusion and oral and maxillofacial surgery.The results are satisfactory, but the side effects have been also widely concerned.The functions and clinical applications of BMPs in bon repair are reviewed.

Background:Upon inhibition of STAT3 signaling pathway,cucurbitacin-I elicits anticancer effect in various malignancies. However,the anticancer effect and underlying mechanism of cucurbitacin-I in gastric cancer is still elusive. Aims:To explore the effect of low nanomolar cucurbitacin-I on cell proliferation,cell cycle and apoptosis in human gastric cancer cells and the underlying mechanism in vitro. Methods:Human gastric adenocarcinoma cell lines AGS and HGC-27 were treated with cucurbitacin-I at low nanomolar concentration. The anti-proliferative effect of cucurbitacin-I was detected by CCK-8 assay and colony formation assay. Flow cytometry was used to assess the cell cycle and apoptosis. Expressions of cell cycle-related proteins,as well as activation of related pathways such as caspase-3 / PARP apoptotic pathway,STAT3, GADD45α and JNK/ p38 MAPK signaling pathways were determined by Western blotting. Results:Cucurbitacin-I markedly inhibited the growth of gastric cancer cells at low nanomolar concentration by inducing G2 / M phase arrest and apoptosis via a STAT3-independent manner. Furthermore,it was revealed that the anticancer effect of cucurbitacin-I was associated with up-regulation of GADD45α,activation of JNK/ p38 MAPK signaling pathway and the subsequent apoptotic events. Conclusions:The present study provides new insights into the mechanism of anticancer effect of cucurbitacin-I, supporting cucurbitacin-I as an attractive therapeutic drug in gastric cancer.

OBJECTIVE: To analyze the therapeutic drug monitoring results of valproic acid( anti- epileptic drug) . METH-ODS: The concentrations of valproic acid in 226 cases in our hospital were determined by fluorescence polarization immunoassay. RESULTS: The results showed that 47. 79% were within the normal referenced concentration range, with average concentration at( 66. 19? 12. 13) ? g? mL- 1, 46. 90% were below the lower limit and 5. 31% were above the upper limit. CONCLUS-ION: The monitoring results are far from satisfactory. It is advisable to adopt individualized administration so as to achieve the goal of treatment.

Objective To analyze the clinical features and causes of benign paroxysmal positional vertigo(BPPV).Methods The clinical data of 521 patients with BPPV were collected.The percentage of patients with BPPV in all the outpatients with dizziness and vertigo,the clinical characteristics of BPPV,and the prevalence of comorbidity with migraine were analyzed.Results BPPV accounted for 35.7% of patients with vertigo and 12.1% of patients with dizziness.Among the 521 patients with BPPV,158(30.3%)were male and 363(69.7%)were female,with an average age of(57.5?12.8)years old(20 to 93 years old).Right semicircular canals were involved in 323 cases(62.0%),and left semicircular canals in 187 cases(35.9%).The latency period of nystagmus of vertical canals was(3.22?2.37)s,and time of persistence was(8.31?7.98)s.The latency period of nystagmus of horizontal canals was(2.33?1.50)s,and the time of persistence was(14.77?11.40)s.There were significant differences in the latency period of nystagmus and time of persistence between the vertical and horizontal canals(P=0.001 and P=0.000).Fifteen patients(2.9%)had a history of head trauma and 39(7.5%)were complicated with migraine.Conclusion BPPV is prevalent in patients with dizziness and vertigo,and misdiagnosis and missed diagnosis should be avoided.

Objective To assess the effectiveness and safety of hyperthermia combined with chemotherapy(HCT)compared with chemotherapy (CT)for ovarian cancer. Methods Related literatures were searched by two independent investigators from the following electronic databases:PubMed,Cochrane Library,EMbase,VIP,WanFang Data,CNKI and CBM.Then the meta?analysis was performed by using RevMan 5.3 software. Results A total of 10 RCTs involving 546 patients were included. The results of Meta?analysis showed that the HCT group was superior to the CT group in the effective rate,tumor control rate,effective rate of CA125,ascites control rate and PD,with significant differences(P<0.05). There was no significant difference in the improvement rate of life quality and the incidences of myelosuppression,severe myelosuppression,severe nausea and vomiting,severe constipation and diarrhea,liver and renal damage(P>0.05). Conclusion Compared with CT,HCT can significantly increase short?term curative effect,ameliorate the quality of life,and it does not increase the incidence of adverse reactions. HCT is worthy of clinical applica?tion.

Objective To study the changes of intestinal transit rate, quantity of enterochromaffin cells and expressions of 5-hydroxytryptamine receptor 3 (5-HT3R) in SD rats at different ages, and to investigate the possible mechanism of gastrointestinal dysfunction in aged rats. Methods Eighty healthy SD rats were divided into five groups: three months age, nine months age, eighteen months age, twenty-four months age and thirty months age,and there were sixteen rats in each group. The intestinal transit rate was detected. Immunohistochemistry staining was used to test the quality of chromaffin cells in mucosa and submucosa of jejunum, ileum and colon and to detect the expressions of 5-HT3R in intestinal myenteric plexus. Results The intestinal transit rate was significantly lower in thirty months age group than in three months age group [(52.1±9.8)% vs. (67.2±13.5)%, t=7.013, P=0.001]. In thirty months age group, the quality of chromaffin cells in mucosa and submueosa of jejunum, ileum and colon were 11.1±3.0, 10.6±1.9, 10.2±4.3, respectively, which were reduced compared with three months age group (22.9±6.2, 25.8±7.1, 23.0±5.7, t=3. 640,3. 384,4. 154, all P<0.01). The expressions of 5-HT3R in myenteric plexus of jejunum and ileum were reduced in thirty months age group than in nine months age group [4.8±1.4, 9.3±4.2 vs. 8.9±1.5, 14.5±5.3;t=3.464, 3.003,all P<0.01]. The expression of 5-HT3R in colon myenteric plexus were 5.0±1.3 and 9.0±1.7 in thirty months age group and three months age group, respectively (t=4.549,P<0.001). Conclusions In aged rats, the intestinal transit rate, quantity of enterochromaffin cells and expressions of 5-HT3R are decreased with ageing, and the gastrointestinal dysfunction may be associated with the changing of the quantity of enterochromaffin cells and expressions of 5-HT3R in myenteric plexus.

Objective:To compare the pharmacokinetics and relative bioavailability between matrine sustained release tablet,matrine conventional capsule and matrine injection. Methods: Dogs were given single oral dose and multiple doses of matrine sustained release tablet, capsule or injection in a randomized crossover way. Matrine concentrations in dog plasma were determined by RP-HPLC method. Results: The results showed that the t max and c max were 300 min and (5.088?0.490) ?g/ml for matrine sustained release tablet, (85?12) min and (6.360?0.215) ?g/ml for the conventional capsule, and 10 min and (6.500?0.404) ?g/ml for the injection. The relative bioavailability of the sustained release tablet was (153.7?9.4)% compared with that of the conventional capsule; the absolute bioavailability of the sustained release tablet was (73.5?14.2)% compared with that of the injecetion. The in vivo absorption rate of the sustained release tablet was significantly correlated with the in vitro release rate(r=0.981 2,P